Reviewed, UniProtKB/Swiss-Prot Q12931 (TRAP1_HUMAN)

Names and origin

Protein names

Recommended name:
    Heat shock protein 75 kDa, mitochondrial
      Short name=HSP 75
Alternative name(s):
    Tumor necrosis factor type 1 receptor-associated protein
      Short name=TRAP-1
    TNFR-associated protein 1

Gene names

Name:	TRAP1
Synonyms:	HSP75

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	704 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Chaperone that expresses an ATPase activity.
Subunit structure	Binds to the intracellular domain of tumor necrosis factor type 1 receptor. Binds to RB1.
Subcellular location	Mitochondrion.
Tissue specificity	Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.
Sequence similarities	Belongs to the heat shock protein 90 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Polymorphism
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tumor necrosis factor receptor binding Ref.3 Non-traceable author statement. Source: UniProtKB unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 59	59	Mitochondrion By similarity
	Chain	60 – 704	645	Heat shock protein 75 kDa, mitochondrial		PRO_0000013604
Sites
	Binding site	119	1	ATP By similarity
	Binding site	158	1	ATP By similarity
	Binding site	171	1	ATP By similarity
	Binding site	205	1	ATP; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	87	1	N6-acetyllysine By similarity
	Modified residue	366	1	Phosphotyrosine Ref.7
	Modified residue	494	1	Phosphothreonine Ref.8
Natural variations
	Natural variant	307	1	R → G: dbSNP rs13926. Ref.1 Ref.3		VAR_016108
	Natural variant	395	1	D → E: dbSNP rs1136948. Ref.5		VAR_049625
	Natural variant	692	1	R → H: dbSNP rs2791.		VAR_049626
Experimental info
	Sequence conflict	17 – 19	3	PLL → ALR in AAA87704. Ref.3
	Sequence conflict	53	1	L → M in AAC02679. Ref.5
	Sequence conflict	475 – 476	2	Missing in AAC02679. Ref.5
	Sequence conflict	488 – 491	4	SRMR → AHW in AAC02679. Ref.5
	Sequence conflict	656 – 704	49	QLRAS…ALERH → HCAQASLAWLSCWWIRYTRT P Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q12931-1 [UniParc]. Last modified June 7, 2005. Version 3. Checksum: 4B16DE3D2B9E0285	FASTA	704	80,110
	10 20 30 40 50 60 MARELRALLL WGRRLRPLLR APALAAVPGG KPILCPRRTT AQLGPRRNPA WSLQAGRLFS 70 80 90 100 110 120 TQTAEDKEEP LHSIISSTES VQGSTSKHEF QAETKKLLDI VARSLYSEKE VFIRELISNA 130 140 150 160 170 180 SDALEKLRHK LVSDGQALPE MEIHLQTNAE KGTITIQDTG IGMTQEELVS NLGTIARSGS 190 200 210 220 230 240 KAFLDALQNQ AEASSKIIGQ FGVGFYSAFM VADRVEVYSR SAAPGSLGYQ WLSDGSGVFE 250 260 270 280 290 300 IAEASGVRTG TKIIIHLKSD CKEFSSEARV RDVVTKYSNF VSFPLYLNGR RMNTLQAIWM 310 320 330 340 350 360 MDPKDVREWQ HEEFYRYVAQ AHDKPRYTLH YKTDAPLNIR SIFYVPDMKP SMFDVSRELG 370 380 390 400 410 420 SSVALYSRKV LIQTKATDIL PKWLRFIRGV VDSEDIPLNL SRELLQESAL IRKLRDVLQQ 430 440 450 460 470 480 RLIKFFIDQS KKDAEKYAKF FEDYGLFMRE GIVTATEQEV KEDIAKLLRY ESSALPSGQL 490 500 510 520 530 540 TSLSEYASRM RAGTRNIYYL CAPNRHLAEH SPYYEAMKKK DTEVLFCFEQ FDELTLLHLR 550 560 570 580 590 600 EFDKKKLISV ETDIVVDHYK EEKFEDRSPA AECLSEKETE ELMAWMRNVL GSRVTNVKVT 610 620 630 640 650 660 LRLDTHPAMV TVLEMGAARH FLRMQQLAKT QEERAQLLQP TLEINPRHAL IKKLNQLRAS 670 680 690 700 EPGLAQLLVD QIYENAMIAA GLVDDPRAMV GRLNELLVKA LERH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A direct interaction between EXT proteins and glycosyltransferases is defective in hereditary multiple exostoses." Simmons A.D., Musy M.M., Lopes C.S., Hwang L.-Y., Yang Y.-P., Lovett M. Hum. Mol. Genet. 8:2155-2164(1999) [PubMed: 10545594] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLY-307.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Eye.
[3]	"Identification of a protein with homology to hsp90 that binds the type 1 tumor necrosis factor receptor." Song H.Y., Dunbar J.D., Zhang Y.X., Guo D., Donner D.B. J. Biol. Chem. 270:3574-3581(1995) [PubMed: 7876093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-704, VARIANT GLY-307.
[4]	Ricke D.O., Bruce D., Mundt M., Doggett N., Munk C., Saunders E., Robinson D., Jones M., Buckingham J., Chasteen L., Thompson S., Goodwin L., Bryant J., Tesmer J., Meincke L., Longmire J., White S., Ueng S. , Tatum O., Campbell C., Fawcett J., Maltbie M., Misra M., Deaven L. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 32-677.
[5]	"A new member of the hsp90 family of molecular chaperones interacts with the retinoblastoma protein during mitosis and after heat shock." Chen C.-F., Chen Y., Dai K., Chen P.-L., Riley D.J., Lee W.-H. Mol. Cell. Biol. 16:4691-4699(1996) [PubMed: 8756626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 53-704, VARIANT GLU-395.
[6]	"The hsp90-related protein TRAP1 is a mitochondrial protein with distinct functional properties." Felts S.J., Owen B.A.L., Nguyen P., Trepel J., Donner D.B., Toft D.O. J. Biol. Chem. 275:3305-3312(2000) [PubMed: 10652318] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-366, MASS SPECTROMETRY. Tissue: Epithelium.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-494, MASS SPECTROMETRY.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF154108 mRNA. Translation: AAF15314.1. BC018950 mRNA. Translation: AAH18950.1. BC023585 mRNA. Translation: AAH23585.1. U12595 mRNA. Translation: AAA87704.1. AC005203 Genomic DNA. Translation: AAC24722.1. AF043254 mRNA. Translation: AAC02679.1.
IPI	IPI00030275.
RefSeq	NP_057376.2.
UniGene	Hs.30345
3D structure databases
HSSP	HSSP built from PDB template 1AMW based on UniProtKB P02829.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6250N.
IntAct	Q12931. 2 interactions.
PTM databases
PhosphoSite	Q12931.
2-D gel databases
REPRODUCTION-2DPAGE	IPI00030275.
Proteomic databases
PeptideAtlas	Q12931.
PRIDE	Q12931.
Genome annotation databases
Ensembl	ENSG00000126602. Homo sapiens. [Contig view]
GeneID	10131.
KEGG	hsa:10131.
NMPDR	fig|9606.3.peg.11646.
Organism-specific databases
GeneCards	GC16M003648.
H-InvDB	HIX0012774. HIX0012776.
HGNC	HGNC:16264. TRAP1.
HPA	CAB004281.
MIM	606219. gene.
PharmGKB	PA36781.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q12931.
HOVERGEN	Q12931.
OMA	Q12931. TEVLFCY.
Gene expression databases
Bgee	Q12931.
CleanEx	HS_TRAP1.
GermOnline	ENSG00000126602. Homo sapiens.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR019805. Heat_shock_protein_90_CS. IPR001404. Hsp90. [Graphical view]
PANTHER	PTHR11528. Hsp90. 1 hit.
Pfam	PF02518. HATPase_c. 1 hit. PF00183. HSP90. 4 hits. [Graphical view]
PIRSF	PIRSF002583. Hsp90. 1 hit.
PRINTS	PR00775. HEATSHOCK90.
SMART	SM00387. HATPase_c. 1 hit. [Graphical view]
PROSITE	PS00298. HSP90. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	38323.
SOURCE	Search...

Entry information

Entry name

TRAP1_HUMAN

Accession

Primary (citable) accession number: Q12931
Secondary accession number(s): O43642, O75235, Q9UHL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	June 7, 2005
Last modified:	June 16, 2009
This is version 93 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)