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Q12918 (KLRB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Killer cell lectin-like receptor subfamily B member 1
Alternative name(s):
C-type lectin domain family 5 member B
HNKR-P1a
Short name=NKR-P1A
Natural killer cell surface protein P1A
CD_antigen=CD161
Gene names
Name:KLRB1
Synonyms:CLEC5B, NKRP1A
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length225 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. Ref.1 Ref.7 Ref.8

Subunit structure

Homodimer; disulfide-linked. Interacts with acid sphingomyelinase/SMPD1. Ref.1 Ref.5 Ref.6 Ref.7 Ref.9

Subcellular location

Membrane; Single-pass type II membrane protein Potential.

Tissue specificity

Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype. Ref.1 Ref.4

Induction

By IL12/interleukin-12 in NK cells. Ref.3

Post-translational modification

N-glycosylated. Contains sialic acid residues. Ref.1

Sequence similarities

Contains 1 C-type lectin domain.

Ontologies

Keywords
   Cellular componentMembrane
   Coding sequence diversityPolymorphism
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   LigandLectin
   Molecular functionReceptor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell surface receptor signaling pathway

Traceable author statement Ref.1. Source: ProtInc

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Traceable author statement Ref.1. Source: ProtInc

   Molecular_functioncarbohydrate binding

Traceable author statement Ref.1. Source: ProtInc

transmembrane signaling receptor activity

Traceable author statement Ref.1. Source: ProtInc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 225225Killer cell lectin-like receptor subfamily B member 1
PRO_0000260000

Regions

Topological domain1 – 4545Cytoplasmic Potential
Transmembrane46 – 6621Helical; Signal-anchor for type II membrane protein; Potential
Topological domain67 – 225159Extracellular Potential
Domain101 – 211111C-type lectin

Amino acid modifications

Glycosylation1571N-linked (GlcNAc...) Potential
Disulfide bond94 ↔ 105 By similarity
Disulfide bond122 ↔ 210 By similarity
Disulfide bond189 ↔ 202 By similarity

Natural variations

Natural variant1681I → T. Ref.2
Corresponds to variant rs1135816 [ dbSNP | Ensembl ].
VAR_028981

Sequences

Sequence LengthMass (Da)Tools
Q12918 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 01BFA925445B83B0

FASTA22525,415
        10         20         30         40         50         60 
MDQQAIYAEL NLPTDSGPES SSPSSLPRDV CQGSPWHQFA LKLSCAGIIL LVLVVTGLSV 

        70         80         90        100        110        120 
SVTSLIQKSS IEKCSVDIQQ SRNKTTERPG LLNCPIYWQQ LREKCLLFSH TVNPWNNSLA 

       130        140        150        160        170        180 
DCSTKESSLL LIRDKDELIH TQNLIRDKAI LFWIGLNFSL SEKNWKWING SFLNSNDLEI 

       190        200        210        220 
RGDAKENSCI SISQTSVYSE YCSTEIRWIC QKELTPVRNK VYPDS 

« Hide

References

« Hide 'large scale' references
[1]"Human NKR-P1A: a disulfide-linked homodimer of the C-type lectin superfamily expressed by a subset of NK and T lymphocytes."
Lanier L.L., Chang C., Phillips J.H.
J. Immunol. 153:2417-2428(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GLYCOSYLATION, HOMODIMERIZATION, TISSUE SPECIFICITY, FUNCTION.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-168.
[3]"IL-12-induced up-regulation of NKRP1A expression in human NK cells and consequent NKRP1A-mediated down-regulation of NK cell activation."
Poggi A., Costa P., Tomasello E., Moretta L.
Eur. J. Immunol. 28:1611-1616(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY IL12.
[4]"CD161+ T (NT) cells exist predominantly in human intestinal epithelium as well as in liver."
Iiai T., Watanabe H., Suda T., Okamoto H., Abo T., Hatakeyama K.
Clin. Exp. Immunol. 129:92-98(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Lectin-like transcript 1 is a ligand for the CD161 receptor."
Aldemir H., Prod'homme V., Dumaurier M.-J., Retiere C., Poupon G., Cazareth J., Bihl F., Braud V.M.
J. Immunol. 175:7791-7795(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CLEC2D.
[6]"Lectin-like transcript-1 is a ligand for the inhibitory human NKR-P1A receptor."
Rosen D.B., Bettadapura J., Alsharifi M., Mathew P.A., Warren H.S., Lanier L.L.
J. Immunol. 175:7796-7799(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CLEC2D.
[7]"CD161 (human NKR-P1A) signaling in NK cells involves the activation of acid sphingomyelinase."
Pozo D., Vales-Gomez M., Mavaddat N., Williamson S.C., Chisholm S.E., Reyburn H.
J. Immunol. 176:2397-2406(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SMPD1.
[8]"Recognition of a carbohydrate xenoepitope by human NKRP1A (CD161)."
Christiansen D., Mouhtouris E., Milland J., Zingoni A., Santoni A., Sandrin M.S.
Xenotransplantation 13:440-446(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A LECTIN.
[9]"Characterization of alternatively spliced transcript variants of CLEC2D gene."
Germain C., Bihl F., Zahn S., Poupon G., Dumaurier M.J., Rampanarivo H.H., Padkjaer S.B., Spee P., Braud V.M.
J. Biol. Chem. 285:36207-36215(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CLEC2D.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U11276 mRNA. Translation: AAA21605.1.
BC113997 mRNA. Translation: AAI13998.1.
BC114516 mRNA. Translation: AAI14517.1.
CCDSCCDS8601.1.
PIRI38700.
RefSeqNP_002249.1. NM_002258.2.
UniGeneHs.169824.

3D structure databases

ProteinModelPortalQ12918.
SMRQ12918. Positions 92-214.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ12918. 1 interaction.
STRING9606.ENSP00000229402.

Polymorphism databases

DMDM74722301.

Proteomic databases

PaxDbQ12918.
PRIDEQ12918.

Protocols and materials databases

DNASU3820.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000229402; ENSP00000229402; ENSG00000111796.
GeneID3820.
KEGGhsa:3820.
UCSCuc010sgt.2. human.

Organism-specific databases

CTD3820.
GeneCardsGC12M010255.
HGNCHGNC:6373. KLRB1.
HPAHPA039113.
MIM602890. gene.
neXtProtNX_Q12918.
PharmGKBPA30162.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG307083.
HOGENOMHOG000074586.
HOVERGENHBG052267.
InParanoidQ12918.
KOK06543.
OMANNSLADC.
OrthoDBEOG7KH9M1.
PhylomeDBQ12918.
TreeFamTF337735.

Gene expression databases

BgeeQ12918.
CleanExHS_KLRB1.
GenevestigatorQ12918.

Family and domain databases

Gene3D3.10.100.10. 1 hit.
InterProIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
[Graphical view]
PfamPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMSSF56436. SSF56436. 1 hit.
PROSITEPS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiKLRB1.
GenomeRNAi3820.
NextBio15015.
PROQ12918.
SOURCESearch...

Entry information

Entry nameKLRB1_HUMAN
AccessionPrimary (citable) accession number: Q12918
Secondary accession number(s): Q24K24
Entry history
Integrated into UniProtKB/Swiss-Prot: November 14, 2006
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries