Q12918 (KLRB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Killer cell lectin-like receptor subfamily B member 1

Alternative name(s):
C-type lectin domain family 5 member B
HNKR-P1a
Short name=NKR-P1A
Natural killer cell surface protein P1A
CD_antigen=CD161

Gene names

Name:	KLRB1
Synonyms:	CLEC5B, NKRP1A

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. Ref.1 Ref.7 Ref.8
Subunit structure	Homodimer; disulfide-linked. Interacts with acid sphingomyelinase/SMPD1. Ref.1 Ref.5 Ref.6 Ref.7 Ref.9
Subcellular location	Membrane; Single-pass type II membrane protein Potential.
Tissue specificity	Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype. Ref.1 Ref.4
Induction	By IL12/interleukin-12 in NK cells. Ref.3
Post-translational modification	N-glycosylated. Contains sialic acid residues. Ref.1
Sequence similarities	Contains 1 C-type lectin domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Lectin
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell surface receptor signaling pathway Traceable author statement Ref.1. Source: ProtInc
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	carbohydrate binding Traceable author statement Ref.1. Source: ProtInc transmembrane signaling receptor activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	Killer cell lectin-like receptor subfamily B member 1		PRO_0000260000
Regions
	Topological domain	1 – 45	45	Cytoplasmic Potential
	Transmembrane	46 – 66	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	67 – 225	159	Extracellular Potential
	Domain	101 – 211	111	C-type lectin
Amino acid modifications
	Glycosylation	157	1	N-linked (GlcNAc...) Potential
	Disulfide bond	94 ↔ 105		By similarity
	Disulfide bond	122 ↔ 210		By similarity
	Disulfide bond	189 ↔ 202		By similarity
Natural variations
	Natural variant	168	1	I → T. Ref.2 Corresponds to variant rs1135816 [ dbSNP | Ensembl ].		VAR_028981

Sequences

	Sequence		Length	Mass (Da)
	Q12918 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 01BFA925445B83B0	FASTA	225	25,415
	10 20 30 40 50 60 MDQQAIYAEL NLPTDSGPES SSPSSLPRDV CQGSPWHQFA LKLSCAGIIL LVLVVTGLSV 70 80 90 100 110 120 SVTSLIQKSS IEKCSVDIQQ SRNKTTERPG LLNCPIYWQQ LREKCLLFSH TVNPWNNSLA 130 140 150 160 170 180 DCSTKESSLL LIRDKDELIH TQNLIRDKAI LFWIGLNFSL SEKNWKWING SFLNSNDLEI 190 200 210 220 RGDAKENSCI SISQTSVYSE YCSTEIRWIC QKELTPVRNK VYPDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human NKR-P1A: a disulfide-linked homodimer of the C-type lectin superfamily expressed by a subset of NK and T lymphocytes." Lanier L.L., Chang C., Phillips J.H. J. Immunol. 153:2417-2428(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GLYCOSYLATION, HOMODIMERIZATION, TISSUE SPECIFICITY, FUNCTION.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-168.
[3]	"IL-12-induced up-regulation of NKRP1A expression in human NK cells and consequent NKRP1A-mediated down-regulation of NK cell activation." Poggi A., Costa P., Tomasello E., Moretta L. Eur. J. Immunol. 28:1611-1616(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY IL12.
[4]	"CD161+ T (NT) cells exist predominantly in human intestinal epithelium as well as in liver." Iiai T., Watanabe H., Suda T., Okamoto H., Abo T., Hatakeyama K. Clin. Exp. Immunol. 129:92-98(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Lectin-like transcript 1 is a ligand for the CD161 receptor." Aldemir H., Prod'homme V., Dumaurier M.-J., Retiere C., Poupon G., Cazareth J., Bihl F., Braud V.M. J. Immunol. 175:7791-7795(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CLEC2D.
[6]	"Lectin-like transcript-1 is a ligand for the inhibitory human NKR-P1A receptor." Rosen D.B., Bettadapura J., Alsharifi M., Mathew P.A., Warren H.S., Lanier L.L. J. Immunol. 175:7796-7799(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CLEC2D.
[7]	"CD161 (human NKR-P1A) signaling in NK cells involves the activation of acid sphingomyelinase." Pozo D., Vales-Gomez M., Mavaddat N., Williamson S.C., Chisholm S.E., Reyburn H. J. Immunol. 176:2397-2406(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SMPD1.
[8]	"Recognition of a carbohydrate xenoepitope by human NKRP1A (CD161)." Christiansen D., Mouhtouris E., Milland J., Zingoni A., Santoni A., Sandrin M.S. Xenotransplantation 13:440-446(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A LECTIN.
[9]	"Characterization of alternatively spliced transcript variants of CLEC2D gene." Germain C., Bihl F., Zahn S., Poupon G., Dumaurier M.J., Rampanarivo H.H., Padkjaer S.B., Spee P., Braud V.M. J. Biol. Chem. 285:36207-36215(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CLEC2D.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

NKRP1

Cross-references

Sequence databases
EMBL GenBank DDBJ	U11276 mRNA. Translation: AAA21605.1. BC113997 mRNA. Translation: AAI13998.1. BC114516 mRNA. Translation: AAI14517.1.
IPI	IPI00032471.
PIR	I38700.
RefSeq	NP_002249.1. NM_002258.2.
UniGene	Hs.169824.
3D structure databases
HSSP	HSSP built from PDB template 1HQ8 based on UniProtKB O54709.
ProteinModelPortal	Q12918.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q12918. 1 interaction.
STRING	9606.ENSP00000229402.
Polymorphism databases
DMDM	74722301.
Proteomic databases
PaxDb	Q12918.
PRIDE	Q12918.
Protocols and materials databases
DNASU	3820.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000229402; ENSP00000229402; ENSG00000111796.
GeneID	3820.
KEGG	hsa:3820.
UCSC	uc010sgt.2. human.
Organism-specific databases
CTD	3820.
GeneCards	GC12M009837.
HGNC	HGNC:6373. KLRB1.
HPA	HPA039113.
MIM	602890. gene.
neXtProt	NX_Q12918.
PharmGKB	PA30162.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG307083.
HOGENOM	HOG000074586.
HOVERGEN	HBG052267.
InParanoid	Q12918.
KO	K06543.
OMA	NNSLADC.
OrthoDB	EOG4NP74P.
PhylomeDB	Q12918.
Gene expression databases
Bgee	Q12918.
CleanEx	HS_KLRB1.
Genevestigator	Q12918.
GermOnline	ENSG00000111796. Homo sapiens.
Family and domain databases
Gene3D	3.10.100.10. 1 hit.
InterPro	IPR001304. C-type_lectin. IPR016186. C-type_lectin-like. IPR016187. C-type_lectin_fold. [Graphical view]
Pfam	PF00059. Lectin_C. 1 hit. [Graphical view]
SMART	SM00034. CLECT. 1 hit. [Graphical view]
SUPFAM	SSF56436. C-type_lectin_fold. 1 hit.
PROSITE	PS00615. C_TYPE_LECTIN_1. False negative. PS50041. C_TYPE_LECTIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	3820.
NextBio	15015.
SOURCE	Search...

Entry information

Entry name

KLRB1_HUMAN

Accession

Primary (citable) accession number: Q12918
Secondary accession number(s): Q24K24

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2006
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.