Receptor-type tyrosine-protein phosphatase eta precursor

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Reviewed, UniProtKB/Swiss-Prot Q12913 (PTPRJ_HUMAN)

Last modified November 3, 2009. Version 101. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Receptor-type tyrosine-protein phosphatase eta
      Short name=Protein-tyrosine phosphatase eta
      Short name=R-PTP-eta
    EC=3.1.3.48
Alternative name(s):
    HPTP eta
    Protein-tyrosine phosphatase receptor type J
    Density-enhanced phosphatase 1
      Short name=DEP-1
    CD_antigen=CD148

Gene names

Name:	PTPRJ
Synonyms:	DEP1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	1337 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	May contribute to the mechanism of contact inhibition of cell growth.
Catalytic activity	Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate.
Subcellular location	Membrane; Single-pass type I membrane protein.
Post-translational modification	N- and O-glycosylated. Ref.5 Ref.6
Involvement in disease	Defects in PTPRJ are found in cancers of colon, lung, and breast.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily. Contains 9 fibronectin type-III domains. Contains 1 tyrosine-protein phosphatase domain.

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Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Disease	Disease mutation
Domain	Repeat Signal Transmembrane
Molecular function	Hydrolase Protein phosphatase
PTM	Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cell-cell signaling Ref.1 Traceable author statement. Source: ProtInc protein amino acid dephosphorylation Traceable author statement. Source: ProtInc transmembrane receptor protein tyrosine kinase signaling pathway Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cell junction Inferred from direct assay. Source: HPA integral to plasma membrane Ref.1 Traceable author statement. Source: ProtInc nucleus Inferred from direct assay. Source: HPA
Molecular function	protein binding Inferred from physical interaction. Source: IntAct transmembrane receptor protein tyrosine phosphatase activity Traceable author statement. Source: ProtInc
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
ALK	Q9UM73	1	EBI-2264500,EBI-357361
CBL	P22681	1	EBI-2264500,EBI-518228
CDH2	P19022	1	EBI-2264500,EBI-2256711
CSK	P41240	1	EBI-2264500,EBI-1380630
CTNNB1	P35222	1	EBI-2264500,EBI-491549
EGFR	P00533	1	EBI-2264500,EBI-297353
EPOR	P19235	1	EBI-2264500,EBI-617321
ERBB2	P04626	1	EBI-2264500,EBI-641062
GAB1	Q13480	1	EBI-2264500,EBI-517684
GHR	P10912	1	EBI-2264500,EBI-286316
GRIN2B	Q13224	1	EBI-2264500,EBI-2256942
INSR	P06213	1	EBI-2264500,EBI-475899
JAK2	O60674	1	EBI-2264500,EBI-518647
KIT	P10721	1	EBI-2264500,EBI-1379503
LCK	P06239	1	EBI-2264500,EBI-1348
LEPR	P48357	1	EBI-2264500,EBI-518596
Lepr	P48356	1	EBI-2264500,EBI-2257257	From a different organism.
MAPK3	P27361	1	EBI-2264500,EBI-73995
MET	P08581	1	EBI-2264500,EBI-1039152
NSF	P46459	1	EBI-2264500,EBI-712251
NTRK1	P04629	1	EBI-2264500,EBI-1028226
PDGFRB	P09619	1	EBI-2264500,EBI-641237
PDPK1	O15530	1	EBI-2264500,EBI-717097
PKP4	Q99569	1	EBI-2264500,EBI-726447
PPP1R12A	O14974	1	EBI-2264500,EBI-351726
PXN	P49023	1	EBI-2264500,EBI-702209
TEC	P42680	1	EBI-2264500,EBI-1383480
TEK	Q02763	1	EBI-2264500,EBI-2257090
TIE1	P35590	1	EBI-2264500,EBI-2256865
TNK2	Q07912	1	EBI-2264500,EBI-603457
WAS	P42768	1	EBI-2264500,EBI-346375

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 35

Potential

Chain

36 – 1337

1302

Receptor-type tyrosine-protein phosphatase eta

PRO_0000025444

Regions

Topological domain

36 – 975

940

Extracellular Potential

Transmembrane

976 – 996

Potential

Topological domain

997 – 1337

341

Cytoplasmic Potential

Domain

119 – 205

Fibronectin type-III 1

Domain

207 – 291

Fibronectin type-III 2

Domain

271 – 364

Fibronectin type-III 3

Domain

365 – 452

Fibronectin type-III 4

Domain

453 – 538

Fibronectin type-III 5

Domain

540 – 620

Fibronectin type-III 6

Domain

622 – 717

Fibronectin type-III 7

Domain

720 – 811

Fibronectin type-III 8

Domain

816 – 902

Fibronectin type-III 9

Domain

1041 – 1298

258

Tyrosine-protein phosphatase

Sites

Active site

1239

Phosphocysteine intermediate By similarity

Amino acid modifications

Modified residue

1011

Phosphoserine By similarity

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

104

N-linked (GlcNAc...) Potential

Glycosylation

142

N-linked (GlcNAc...) Potential

Glycosylation

172

N-linked (GlcNAc...) Potential

Glycosylation

192

N-linked (GlcNAc...) Potential

Glycosylation

231

N-linked (GlcNAc...) Potential

Glycosylation

258

N-linked (GlcNAc...) Potential

Glycosylation

278

N-linked (GlcNAc...) Potential

Glycosylation

342

N-linked (GlcNAc...) Ref.5

Glycosylation

351

N-linked (GlcNAc...) Ref.5

Glycosylation

376

N-linked (GlcNAc...) Potential

Glycosylation

391

N-linked (GlcNAc...) Ref.5

Glycosylation

396

N-linked (GlcNAc...) Ref.5

Glycosylation

413

N-linked (GlcNAc...) Ref.6

Glycosylation

431

N-linked (GlcNAc...) Potential

Glycosylation

501

N-linked (GlcNAc...) Potential

Glycosylation

525

N-linked (GlcNAc...) Potential

Glycosylation

536

N-linked (GlcNAc...) Potential

Glycosylation

582

N-linked (GlcNAc...) Potential

Glycosylation

603

N-linked (GlcNAc...) Potential

Glycosylation

618

N-linked (GlcNAc...) Potential

Glycosylation

628

N-linked (GlcNAc...) Potential

Glycosylation

637

N-linked (GlcNAc...) Potential

Glycosylation

666

N-linked (GlcNAc...) Potential

Glycosylation

669

N-linked (GlcNAc...) Potential

Glycosylation

761

N-linked (GlcNAc...) Potential

Glycosylation

772

N-linked (GlcNAc...) Potential

Glycosylation

784

N-linked (GlcNAc...) Potential

Glycosylation

790

N-linked (GlcNAc...) Potential

Glycosylation

824

N-linked (GlcNAc...) Potential

Glycosylation

910

N-linked (GlcNAc...) Potential

Glycosylation

937

N-linked (GlcNAc...) Ref.6

Natural variations

Natural variant

214

R → C in colon cancer; somatic mutation. Ref.4

VAR_015905

Natural variant

276

Q → P in colon cancer; somatic mutation. dbSNP rs1566734. Ref.4

VAR_015906

Natural variant

293

A → T: dbSNP rs2229701.

VAR_038414

Natural variant

326

R → Q: dbSNP rs1503185.

VAR_024582

Natural variant

372

V → I: dbSNP rs2229703.

VAR_038415

Natural variant

872

E → D: dbSNP rs4752904. Ref.4 Ref.1 Ref.2

VAR_038416

Natural variant

1235

I → T: dbSNP rs11039554.

VAR_038417

Experimental info

Sequence conflict

261

G → D in AAB36687. Ref.1

Sequence conflict

918 – 929

YNGKL…LGSYR → LQWEAGTSGLLP in BAA07035. Ref.2

Secondary structure

1337

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

Q12913-1 [UniParc].

Last modified February 5, 2008. Version 3.
Checksum: B44F4343FC8FD1B4
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FASTA

1,337

145,941

        10         20         30         40         50         60 
MKPAAREARL PPRSPGLRWA LPLLLLLLRL GQILCAGGTP SPIPDPSVAT VATGENGITQ 

        70         80         90        100        110        120 
ISSTAESFHK QNGTGTPQVE TNTSEDGESS GANDSLRTPE QGSNGTDGAS QKTPSSTGPS 

       130        140        150        160        170        180 
PVFDIKAVSI SPTNVILTWK SNDTAASEYK YVVKHKMENE KTITVVHQPW CNITGLRPAT 

       190        200        210        220        230        240 
SYVFSITPGI GNETWGDPRV IKVITEPIPV SDLRVALTGV RKAALSWSNG NGTASCRVLL 

       250        260        270        280        290        300 
ESIGSHEELT QDSRLQVNIS GLKPGVQYNI NPYLLQSNKT KGDPLGTEGG LDASNTERSR 

       310        320        330        340        350        360 
AGSPTAPVHD ESLVGPVDPS SGQQSRDTEV LLVGLEPGTR YNATVYSQAA NGTEGQPQAI 

       370        380        390        400        410        420 
EFRTNAIQVF DVTAVNISAT SLTLIWKVSD NESSSNYTYK IHVAGETDSS NLNVSEPRAV 

       430        440        450        460        470        480 
IPGLRSSTFY NITVCPVLGD IEGTPGFLQV HTPPVPVSDF RVTVVSTTEI GLAWSSHDAE 

       490        500        510        520        530        540 
SFQMHITQEG AGNSRVEITT NQSIIIGGLF PGTKYCFEIV PKGPNGTEGA SRTVCNRTVP 

       550        560        570        580        590        600 
SAVFDIHVVY VTTTEMWLDW KSPDGASEYV YHLVIESKHG SNHTSTYDKA ITLQGLIPGT 

       610        620        630        640        650        660 
LYNITISPEV DHVWGDPNST AQYTRPSNVS NIDVSTNTTA ATLSWQNFDD ASPTYSYCLL 

       670        680        690        700        710        720 
IEKAGNSSNA TQVVTDIGIT DATVTELIPG SSYTVEIFAQ VGDGIKSLEP GRKSFCTDPA 

       730        740        750        760        770        780 
SMASFDCEVV PKEPALVLKW TCPPGANAGF ELEVSSGAWN NATHLESCSS ENGTEYRTEV 

       790        800        810        820        830        840 
TYLNFSTSYN ISITTVSCGK MAAPTRNTCT TGITDPPPPD GSPNITSVSH NSVKVKFSGF 

       850        860        870        880        890        900 
EASHGPIKAY AVILTTGEAG HPSADVLKYT YEDFKKGASD TYVTYLIRTE EKGRSQSLSE 

       910        920        930        940        950        960 
VLKYEIDVGN ESTTLGYYNG KLEPLGSYRA CVAGFTNITF HPQNKGLIDG AESYVSFSRY 

       970        980        990       1000       1010       1020 
SDAVSLPQDP GVICGAVFGC IFGALVIVTV GGFIFWRKKR KDAKNNEVSF SQIKPKKSKL 

      1030       1040       1050       1060       1070       1080 
IRVENFEAYF KKQQADSNCG FAEEYEDLKL VGISQPKYAA ELAENRGKNR YNNVLPYDIS 

      1090       1100       1110       1120       1130       1140 
RVKLSVQTHS TDDYINANYM PGYHSKKDFI ATQGPLPNTL KDFWRMVWEK NVYAIIMLTK 

      1150       1160       1170       1180       1190       1200 
CVEQGRTKCE EYWPSKQAQD YGDITVAMTS EIVLPEWTIR DFTVKNIQTS ESHPLRQFHF 

      1210       1220       1230       1240       1250       1260 
TSWPDHGVPD TTDLLINFRY LVRDYMKQSP PESPILVHCS AGVGRTGTFI AIDRLIYQIE 

      1270       1280       1290       1300       1310       1320 
NENTVDVYGI VYDLRMHRPL MVQTEDQYVF LNQCVLDIVR SQKDSKVDLI YQNTTAMTIY 

      1330 
ENLAPVTTFG KTNGYIA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of DEP-1, a receptor-like protein-tyrosine-phosphatase, is enhanced with increasing cell density." Oestman A., Yang Q., Tonks N.K. Proc. Natl. Acad. Sci. U.S.A. 91:9680-9684(1994) [PubMed: 7937872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASP-872.
[2]	"Molecular cloning, characterization, and chromosomal localization of a novel protein-tyrosine phosphatase, HPTP eta." Honda H., Inazawa J., Nishida J., Yazaki Y., Hirai H. Blood 84:4186-4194(1994) [PubMed: 7994032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASP-872.
[3]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. Sakaki Y. Nature 440:497-500(2006) [PubMed: 16554811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Ptprj is a candidate for the mouse colon-cancer susceptibility locus Scc1 and is frequently deleted in human cancers." Ruivenkamp C.A.L., van Wezel T., Zanon C., Stassen A.P.M., Vlcek C., Csikos T., Klous A.M., Tripodis N., Perrakis A., Boerrigter L., Groot P.C., Lindeman J., Mooi W.J., Meijjer G.A., Scholten G., Dauwerse H., Paces V., van Zandwijk N., van Ommen G.J.B., Demant P. Nat. Genet. 31:295-300(2002) [PubMed: 12089527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 33-1337, VARIANTS COLON CANCER CYS-214 AND PRO-276, VARIANT ASP-872. Tissue: Colon.
[5]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed: 16335952] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-342; ASN-351; ASN-391 AND ASN-396, MASS SPECTROMETRY. Tissue: Plasma.
[6]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-413 AND ASN-937, MASS SPECTROMETRY. Tissue: Liver.
[7]	"Solution structure of the fourth FN3 domain of human receptor-type tyrosine-protein phosphatase eta." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 366-456.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

U10886 mRNA. Translation: AAB36687.1.
D37781 mRNA. Translation: BAA07035.1.
AC103828 Genomic DNA. No translation available.
AC026975 Genomic DNA. No translation available.
AF387844

AF387843 Genomic DNA. Translation: AAM69432.1.

IPI

IPI00290328.

PIR

I38670.

RefSeq

NP_002834.3.

UniGene

Hs.318547

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CFV	X-ray	2.50	A	1019-1311	[»]
2DLE	NMR	-	A	366-456	[»]
2NZ6	X-ray	2.30	A	1019-1311	[»]

SMR

Q12913. Positions 364-456.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q12913. 31 interactions.

STRING

Q12913.

PTM databases

PhosphoSite

Q12913.

Proteomic databases

PRIDE

Q12913.

Genome annotation databases

Ensembl

ENST00000278456; ENSP00000278456; ENSG00000149177; Homo sapiens. [Genome view]
ENST00000418331; ENSP00000400010; ENSG00000149177; Homo sapiens. [Genome view]
ENST00000440289; ENSP00000409733; ENSG00000149177; Homo sapiens. [Genome view]

GeneID

5795.

UCSC

uc001ngp.2. human.

Organism-specific databases

CTD

5795.

GeneCards

GC11P047958.

H-InvDB

HIX0035923.

HGNC

HGNC:9673. PTPRJ.

HPA

HPA006026.

MIM

600925. gene.

PharmGKB

PA34018.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q12913.

HOVERGEN

Q12913.

OMA

EYRTEVT.

Enzyme and pathway databases

BRENDA

3.1.3.48. 247.

Pathway_Interaction_DB

pdgfrbpathway. PDGFR-beta signaling pathway.
met_pathway. Signaling events activated by Hepatocyte Growth Factor Receptor (c-Met).

Gene expression databases

ArrayExpress

Q12913.

Bgee

Q12913.

CleanEx

HS_PTPRJ.

Genevestigator

Q12913.

GermOnline

ENSG00000149177. Homo sapiens.

Family and domain databases

InterPro

IPR000387. Dual-sp/Tyr_phosphatase.
IPR008957. Fibronectin_typ-III-like_fold.
IPR003961. FN_III.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]

Gene3D

G3DSA:2.60.40.30. FN_III-like. 4 hits.

Pfam

PF00041. fn3. 5 hits.
PF00102. Y_phosphatase. 1 hit.
[Graphical view]

PRINTS

PR00700. PRTYPHPHTASE.

SMART

SM00060. FN3. 8 hits.
SM00194. PTPc. 1 hit.
[Graphical view]

PROSITE

PS50853. FN3. 9 hits.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

22564.

SOURCE

Search...

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Entry information

Entry name

PTPRJ_HUMAN

Accession

Primary (citable) accession number: Q12913
Secondary accession number(s): Q15255, Q8NHM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 101 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

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CD nomenclature of surface proteins of human leucocytes and list of entries

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents