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Reviewed, UniProtKB/Swiss-Prot Q12913 (PTPRJ_HUMAN)

Last modified November 3, 2009. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Receptor-type tyrosine-protein phosphatase eta
      Short name=Protein-tyrosine phosphatase eta
      Short name=R-PTP-eta
    EC=3.1.3.48
Alternative name(s):
    HPTP eta
    Protein-tyrosine phosphatase receptor type J
    Density-enhanced phosphatase 1
      Short name=DEP-1
    CD_antigen=CD148
Gene names
Name: PTPRJ
Synonyms: DEP1
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1337 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

May contribute to the mechanism of contact inhibition of cell growth.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subcellular location

Membrane; Single-pass type I membrane protein.

Post-translational modification

N- and O-glycosylated. Ref.5 Ref.6

Involvement in disease

Defects in PTPRJ are found in cancers of colon, lung, and breast.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.

Contains 9 fibronectin type-III domains.

Contains 1 tyrosine-protein phosphatase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3535 Potential
Chain36 – 13371302Receptor-type tyrosine-protein phosphatase eta
PRO_0000025444

Regions

Topological domain36 – 975940Extracellular Potential
Transmembrane976 – 99621 Potential
Topological domain997 – 1337341Cytoplasmic Potential
Domain119 – 20587Fibronectin type-III 1
Domain207 – 29185Fibronectin type-III 2
Domain271 – 36494Fibronectin type-III 3
Domain365 – 45288Fibronectin type-III 4
Domain453 – 53886Fibronectin type-III 5
Domain540 – 62081Fibronectin type-III 6
Domain622 – 71796Fibronectin type-III 7
Domain720 – 81192Fibronectin type-III 8
Domain816 – 90287Fibronectin type-III 9
Domain1041 – 1298258Tyrosine-protein phosphatase

Sites

Active site12391Phosphocysteine intermediate By similarity

Amino acid modifications

Modified residue10111Phosphoserine By similarity
Glycosylation721N-linked (GlcNAc...) Potential
Glycosylation821N-linked (GlcNAc...) Potential
Glycosylation931N-linked (GlcNAc...) Potential
Glycosylation1041N-linked (GlcNAc...) Potential
Glycosylation1421N-linked (GlcNAc...) Potential
Glycosylation1721N-linked (GlcNAc...) Potential
Glycosylation1921N-linked (GlcNAc...) Potential
Glycosylation2311N-linked (GlcNAc...) Potential
Glycosylation2581N-linked (GlcNAc...) Potential
Glycosylation2781N-linked (GlcNAc...) Potential
Glycosylation3421N-linked (GlcNAc...) Ref.5
Glycosylation3511N-linked (GlcNAc...) Ref.5
Glycosylation3761N-linked (GlcNAc...) Potential
Glycosylation3911N-linked (GlcNAc...) Ref.5
Glycosylation3961N-linked (GlcNAc...) Ref.5
Glycosylation4131N-linked (GlcNAc...) Ref.6
Glycosylation4311N-linked (GlcNAc...) Potential
Glycosylation5011N-linked (GlcNAc...) Potential
Glycosylation5251N-linked (GlcNAc...) Potential
Glycosylation5361N-linked (GlcNAc...) Potential
Glycosylation5821N-linked (GlcNAc...) Potential
Glycosylation6031N-linked (GlcNAc...) Potential
Glycosylation6181N-linked (GlcNAc...) Potential
Glycosylation6281N-linked (GlcNAc...) Potential
Glycosylation6371N-linked (GlcNAc...) Potential
Glycosylation6661N-linked (GlcNAc...) Potential
Glycosylation6691N-linked (GlcNAc...) Potential
Glycosylation7611N-linked (GlcNAc...) Potential
Glycosylation7721N-linked (GlcNAc...) Potential
Glycosylation7841N-linked (GlcNAc...) Potential
Glycosylation7901N-linked (GlcNAc...) Potential
Glycosylation8241N-linked (GlcNAc...) Potential
Glycosylation9101N-linked (GlcNAc...) Potential
Glycosylation9371N-linked (GlcNAc...) Ref.6

Natural variations

Natural variant2141R → C in colon cancer; somatic mutation. Ref.4
VAR_015905
Natural variant2761Q → P in colon cancer; somatic mutation. dbSNP rs1566734. Ref.4
VAR_015906
Natural variant2931A → T: dbSNP rs2229701.
VAR_038414
Natural variant3261R → Q: dbSNP rs1503185.
VAR_024582
Natural variant3721V → I: dbSNP rs2229703.
VAR_038415
Natural variant8721E → D: dbSNP rs4752904. Ref.4 Ref.1 Ref.2
VAR_038416
Natural variant12351I → T: dbSNP rs11039554.
VAR_038417

Experimental info

Sequence conflict2611G → D in AAB36687. Ref.1
Sequence conflict918 – 92912YNGKL…LGSYR → LQWEAGTSGLLP in BAA07035. Ref.2

Secondary structure

........................................... 1337
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q12913-1 [UniParc].

Last modified February 5, 2008. Version 3.
Checksum: B44F4343FC8FD1B4

FASTA1,337145,941
        10         20         30         40         50         60 
MKPAAREARL PPRSPGLRWA LPLLLLLLRL GQILCAGGTP SPIPDPSVAT VATGENGITQ 

        70         80         90        100        110        120 
ISSTAESFHK QNGTGTPQVE TNTSEDGESS GANDSLRTPE QGSNGTDGAS QKTPSSTGPS 

       130        140        150        160        170        180 
PVFDIKAVSI SPTNVILTWK SNDTAASEYK YVVKHKMENE KTITVVHQPW CNITGLRPAT 

       190        200        210        220        230        240 
SYVFSITPGI GNETWGDPRV IKVITEPIPV SDLRVALTGV RKAALSWSNG NGTASCRVLL 

       250        260        270        280        290        300 
ESIGSHEELT QDSRLQVNIS GLKPGVQYNI NPYLLQSNKT KGDPLGTEGG LDASNTERSR 

       310        320        330        340        350        360 
AGSPTAPVHD ESLVGPVDPS SGQQSRDTEV LLVGLEPGTR YNATVYSQAA NGTEGQPQAI 

       370        380        390        400        410        420 
EFRTNAIQVF DVTAVNISAT SLTLIWKVSD NESSSNYTYK IHVAGETDSS NLNVSEPRAV 

       430        440        450        460        470        480 
IPGLRSSTFY NITVCPVLGD IEGTPGFLQV HTPPVPVSDF RVTVVSTTEI GLAWSSHDAE 

       490        500        510        520        530        540 
SFQMHITQEG AGNSRVEITT NQSIIIGGLF PGTKYCFEIV PKGPNGTEGA SRTVCNRTVP 

       550        560        570        580        590        600 
SAVFDIHVVY VTTTEMWLDW KSPDGASEYV YHLVIESKHG SNHTSTYDKA ITLQGLIPGT 

       610        620        630        640        650        660 
LYNITISPEV DHVWGDPNST AQYTRPSNVS NIDVSTNTTA ATLSWQNFDD ASPTYSYCLL 

       670        680        690        700        710        720 
IEKAGNSSNA TQVVTDIGIT DATVTELIPG SSYTVEIFAQ VGDGIKSLEP GRKSFCTDPA 

       730        740        750        760        770        780 
SMASFDCEVV PKEPALVLKW TCPPGANAGF ELEVSSGAWN NATHLESCSS ENGTEYRTEV 

       790        800        810        820        830        840 
TYLNFSTSYN ISITTVSCGK MAAPTRNTCT TGITDPPPPD GSPNITSVSH NSVKVKFSGF 

       850        860        870        880        890        900 
EASHGPIKAY AVILTTGEAG HPSADVLKYT YEDFKKGASD TYVTYLIRTE EKGRSQSLSE 

       910        920        930        940        950        960 
VLKYEIDVGN ESTTLGYYNG KLEPLGSYRA CVAGFTNITF HPQNKGLIDG AESYVSFSRY 

       970        980        990       1000       1010       1020 
SDAVSLPQDP GVICGAVFGC IFGALVIVTV GGFIFWRKKR KDAKNNEVSF SQIKPKKSKL 

      1030       1040       1050       1060       1070       1080 
IRVENFEAYF KKQQADSNCG FAEEYEDLKL VGISQPKYAA ELAENRGKNR YNNVLPYDIS 

      1090       1100       1110       1120       1130       1140 
RVKLSVQTHS TDDYINANYM PGYHSKKDFI ATQGPLPNTL KDFWRMVWEK NVYAIIMLTK 

      1150       1160       1170       1180       1190       1200 
CVEQGRTKCE EYWPSKQAQD YGDITVAMTS EIVLPEWTIR DFTVKNIQTS ESHPLRQFHF 

      1210       1220       1230       1240       1250       1260 
TSWPDHGVPD TTDLLINFRY LVRDYMKQSP PESPILVHCS AGVGRTGTFI AIDRLIYQIE 

      1270       1280       1290       1300       1310       1320 
NENTVDVYGI VYDLRMHRPL MVQTEDQYVF LNQCVLDIVR SQKDSKVDLI YQNTTAMTIY 

      1330 
ENLAPVTTFG KTNGYIA 

« Hide

References

« Hide 'large scale' references
[1]"Expression of DEP-1, a receptor-like protein-tyrosine-phosphatase, is enhanced with increasing cell density."
Oestman A., Yang Q., Tonks N.K.
Proc. Natl. Acad. Sci. U.S.A. 91:9680-9684(1994) [PubMed: 7937872] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASP-872.
[2]"Molecular cloning, characterization, and chromosomal localization of a novel protein-tyrosine phosphatase, HPTP eta."
Honda H., Inazawa J., Nishida J., Yazaki Y., Hirai H.
Blood 84:4186-4194(1994) [PubMed: 7994032] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASP-872.
[3]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed: 16554811] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Ptprj is a candidate for the mouse colon-cancer susceptibility locus Scc1 and is frequently deleted in human cancers."
Ruivenkamp C.A.L., van Wezel T., Zanon C., Stassen A.P.M., Vlcek C., Csikos T., Klous A.M., Tripodis N., Perrakis A., Boerrigter L., Groot P.C., Lindeman J., Mooi W.J., Meijjer G.A., Scholten G., Dauwerse H., Paces V., van Zandwijk N., van Ommen G.J.B., Demant P.
Nat. Genet. 31:295-300(2002) [PubMed: 12089527] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 33-1337, VARIANTS COLON CANCER CYS-214 AND PRO-276, VARIANT ASP-872.
Tissue: Colon.
[5]"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
J. Proteome Res. 4:2070-2080(2005) [PubMed: 16335952] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-342; ASN-351; ASN-391 AND ASN-396, MASS SPECTROMETRY.
Tissue: Plasma.
[6]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-413 AND ASN-937, MASS SPECTROMETRY.
Tissue: Liver.
[7]"Solution structure of the fourth FN3 domain of human receptor-type tyrosine-protein phosphatase eta."
RIKEN structural genomics initiative (RSGI)
Submitted (OCT-2006) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 366-456.
+Additional computationally mapped references.

Cross-references

Sequence databases

U10886 mRNA. Translation: AAB36687.1.
D37781 mRNA. Translation: BAA07035.1.
AC103828 Genomic DNA. No translation available.
AC026975 Genomic DNA. No translation available.
AF387844 expand/collapse EMBL AC list , AF387823, AF387824, AF387825, AF387826, AF387827, AF387828, AF387829, AF387830, AF387831, AF387832, AF387833, AF387834, AF387835, AF387836, AF387837, AF387838, AF387839, AF387840, AF387841, AF387842, AF387843 Genomic DNA. Translation: AAM69432.1.
IPIIPI00290328.
PIRI38670.
RefSeqNP_002834.3.
UniGeneHs.318547

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2CFVX-ray2.50A1019-1311[»]
2DLENMR-A366-456[»]
2NZ6X-ray2.30A1019-1311[»]
SMRQ12913. Positions 364-456.
ModBaseSearch...

Protein-protein interaction databases

IntActQ12913. 31 interactions.
STRINGQ12913.

PTM databases

PhosphoSiteQ12913.

Proteomic databases

PRIDEQ12913.

Genome annotation databases

EnsemblENST00000278456; ENSP00000278456; ENSG00000149177; Homo sapiens. [Genome view]
ENST00000418331; ENSP00000400010; ENSG00000149177; Homo sapiens. [Genome view]
ENST00000440289; ENSP00000409733; ENSG00000149177; Homo sapiens. [Genome view]
GeneID5795.
UCSCuc001ngp.2. human.

Organism-specific databases

CTD5795.
GeneCardsGC11P047958.
H-InvDBHIX0035923.
HGNCHGNC:9673. PTPRJ.
HPAHPA006026.
MIM600925. gene.
PharmGKBPA34018.
GenAtlasSearch...

Phylogenomic databases

HOGENOMQ12913.
HOVERGENQ12913.
OMAEYRTEVT.

Enzyme and pathway databases

BRENDA3.1.3.48. 247.
Pathway_Interaction_DBpdgfrbpathway. PDGFR-beta signaling pathway.
met_pathway. Signaling events activated by Hepatocyte Growth Factor Receptor (c-Met).

Gene expression databases

ArrayExpressQ12913.
BgeeQ12913.
CleanExHS_PTPRJ.
GenevestigatorQ12913.
GermOnlineENSG00000149177. Homo sapiens.

Family and domain databases

InterProIPR000387. Dual-sp/Tyr_phosphatase.
IPR008957. Fibronectin_typ-III-like_fold.
IPR003961. FN_III.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
Gene3DG3DSA:2.60.40.30. FN_III-like. 4 hits.
PfamPF00041. fn3. 5 hits.
PF00102. Y_phosphatase. 1 hit.
[Graphical view]
PRINTSPR00700. PRTYPHPHTASE.
SMARTSM00060. FN3. 8 hits.
SM00194. PTPc. 1 hit.
[Graphical view]
PROSITEPS50853. FN3. 9 hits.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio22564.
SOURCESearch...

Entry information

Entry namePTPRJ_HUMAN
AccessionPrimary (citable) accession number: Q12913
Secondary accession number(s): Q15255, Q8NHM2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 5, 2008
Last modified: November 3, 2009
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents