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Protein

Vesicular integral-membrane protein VIP36

Gene

LMAN2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role as an intracellular lectin in the early secretory pathway. Interacts with N-acetyl-D-galactosamine and high-mannose type glycans and may also bind to O-linked glycans. Involved in the transport and sorting of glycoproteins carrying high mannose-type glycans (By similarity).By similarity

Cofactori

Ca2+By similarityNote: Binds 2 calcium ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei96 – 961CarbohydratePROSITE-ProRule annotation
Binding sitei131 – 1311CarbohydratePROSITE-ProRule annotation
Metal bindingi162 – 1621CalciumPROSITE-ProRule annotation
Metal bindingi164 – 1641Calcium; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi166 – 1661CalciumPROSITE-ProRule annotation
Binding sitei190 – 1901CarbohydratePROSITE-ProRule annotation
Metal bindingi193 – 1931CalciumPROSITE-ProRule annotation

GO - Molecular functioni

  • carbohydrate binding Source: UniProtKB
  • glycoprotein binding Source: UniProtKB
  • heat shock protein binding Source: UniProtKB
  • mannose binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • positive regulation of phagocytosis Source: UniProtKB
  • protein transport Source: UniProtKB-KW
  • retrograde vesicle-mediated transport, Golgi to ER Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Calcium, Lectin, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-HSA-5694530. Cargo concentration in the ER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular integral-membrane protein VIP36
Alternative name(s):
Glycoprotein GP36b
Lectin mannose-binding 2
Vesicular integral-membrane protein 36
Short name:
VIP36
Gene namesi
Name:LMAN2
Synonyms:C5orf8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:16986. LMAN2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini45 – 322278LumenalSequence analysisAdd
BLAST
Transmembranei323 – 34523HelicalSequence analysisAdd
BLAST
Topological domaini346 – 35611CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell surface Source: UniProtKB
  • endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB
  • endoplasmic reticulum-Golgi intermediate compartment membrane Source: UniProtKB-SubCell
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA25919.

Polymorphism and mutation databases

BioMutaiLMAN2.
DMDMi21264108.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4444Sequence analysisCombined sourcesAdd
BLAST
Chaini45 – 356312Vesicular integral-membrane protein VIP36PRO_0000017666Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi183 – 1831N-linked (GlcNAc...)1 Publication
Disulfide bondi202 ↔ 239PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ12907.
MaxQBiQ12907.
PaxDbiQ12907.
PeptideAtlasiQ12907.
PRIDEiQ12907.
TopDownProteomicsiQ12907.

PTM databases

iPTMnetiQ12907.
PhosphoSiteiQ12907.
SwissPalmiQ12907.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiQ12907.
CleanExiHS_LMAN2.
ExpressionAtlasiQ12907. baseline and differential.
GenevisibleiQ12907. HS.

Organism-specific databases

HPAiHPA003927.

Interactioni

GO - Molecular functioni

  • glycoprotein binding Source: UniProtKB
  • heat shock protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi116159. 29 interactions.
IntActiQ12907. 7 interactions.
MINTiMINT-4999928.
STRINGi9606.ENSP00000303366.

Structurei

3D structure databases

ProteinModelPortaliQ12907.
SMRiQ12907. Positions 51-301.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini52 – 276225L-type lectin-likePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni164 – 1663Carbohydrate bindingPROSITE-ProRule annotation
Regioni260 – 2623Carbohydrate bindingPROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 L-type lectin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3839. Eukaryota.
ENOG410YH8V. LUCA.
GeneTreeiENSGT00530000062977.
HOGENOMiHOG000164540.
HOVERGENiHBG052334.
InParanoidiQ12907.
KOiK10082.
OMAiIWNRVPC.
OrthoDBiEOG718KCT.
PhylomeDBiQ12907.
TreeFamiTF313311.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR005052. Lectin_leg.
[Graphical view]
PfamiPF03388. Lectin_leg-like. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51328. L_LECTIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12907-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAEGWIWRW GWGRRCLGRP GLLGPGPGPT TPLFLLLLLG SVTADITDGN
60 70 80 90 100
SEHLKREHSL IKPYQGVGSS SMPLWDFQGS TMLTSQYVRL TPDERSKEGS
110 120 130 140 150
IWNHQPCFLK DWEMHVHFKV HGTGKKNLHG DGIALWYTRD RLVPGPVFGS
160 170 180 190 200
KDNFHGLAIF LDTYPNDETT ERVFPYISVM VNNGSLSYDH SKDGRWTELA
210 220 230 240 250
GCTADFRNRD HDTFLAVRYS RGRLTVMTDL EDKNEWKNCI DITGVRLPTG
260 270 280 290 300
YYFGASAGTG DLSDNHDIIS MKLFQLMVEH TPDEESIDWT KIEPSVNFLK
310 320 330 340 350
SPKDNVDDPT GNFRSGPLTG WRVFLLLLCA LLGIVVCAVV GAVVFQKRQE

RNKRFY
Length:356
Mass (Da):40,229
Last modified:November 1, 1996 - v1
Checksum:i196193C8E655141C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti266 – 2661H → R in BAD96329 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10362 mRNA. Translation: AAA19572.1.
AK222609 mRNA. Translation: BAD96329.1.
BC017263 mRNA. Translation: AAH17263.1.
CCDSiCCDS4417.1.
PIRiG01447.
RefSeqiNP_006807.1. NM_006816.2.
UniGeneiHs.75864.

Genome annotation databases

EnsembliENST00000303127; ENSP00000303366; ENSG00000169223.
GeneIDi10960.
KEGGihsa:10960.
UCSCiuc003mge.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10362 mRNA. Translation: AAA19572.1.
AK222609 mRNA. Translation: BAD96329.1.
BC017263 mRNA. Translation: AAH17263.1.
CCDSiCCDS4417.1.
PIRiG01447.
RefSeqiNP_006807.1. NM_006816.2.
UniGeneiHs.75864.

3D structure databases

ProteinModelPortaliQ12907.
SMRiQ12907. Positions 51-301.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116159. 29 interactions.
IntActiQ12907. 7 interactions.
MINTiMINT-4999928.
STRINGi9606.ENSP00000303366.

PTM databases

iPTMnetiQ12907.
PhosphoSiteiQ12907.
SwissPalmiQ12907.

Polymorphism and mutation databases

BioMutaiLMAN2.
DMDMi21264108.

Proteomic databases

EPDiQ12907.
MaxQBiQ12907.
PaxDbiQ12907.
PeptideAtlasiQ12907.
PRIDEiQ12907.
TopDownProteomicsiQ12907.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000303127; ENSP00000303366; ENSG00000169223.
GeneIDi10960.
KEGGihsa:10960.
UCSCiuc003mge.4. human.

Organism-specific databases

CTDi10960.
GeneCardsiLMAN2.
HGNCiHGNC:16986. LMAN2.
HPAiHPA003927.
MIMi609551. gene.
neXtProtiNX_Q12907.
PharmGKBiPA25919.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3839. Eukaryota.
ENOG410YH8V. LUCA.
GeneTreeiENSGT00530000062977.
HOGENOMiHOG000164540.
HOVERGENiHBG052334.
InParanoidiQ12907.
KOiK10082.
OMAiIWNRVPC.
OrthoDBiEOG718KCT.
PhylomeDBiQ12907.
TreeFamiTF313311.

Enzyme and pathway databases

ReactomeiR-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-HSA-5694530. Cargo concentration in the ER.

Miscellaneous databases

ChiTaRSiLMAN2. human.
GeneWikiiLMAN2.
GenomeRNAii10960.
PROiQ12907.
SOURCEiSearch...

Gene expression databases

BgeeiQ12907.
CleanExiHS_LMAN2.
ExpressionAtlasiQ12907. baseline and differential.
GenevisibleiQ12907. HS.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR005052. Lectin_leg.
[Graphical view]
PfamiPF03388. Lectin_leg-like. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51328. L_LECTIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human GP36b glycoprotein of the endoplasmic reticulum."
    Hartmann E., Reimann B., Goerlich D., Rapoport T.A., Prehn S.
    Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Smooth muscle.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "VIP36 localisation to the early secretory pathway."
    Fullekrug J., Scheiffele P., Simons K.
    J. Cell Sci. 112:2813-2821(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry."
    Zhang H., Li X.-J., Martin D.B., Aebersold R.
    Nat. Biotechnol. 21:660-666(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT ASN-183.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. Cited for: CLEAVAGE OF SIGNAL PEPTIDE [LARGE SCALE ANALYSIS] AFTER ALA-44, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLMAN2_HUMAN
AccessioniPrimary (citable) accession number: Q12907
Secondary accession number(s): Q53HH1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.