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Q12905

- ILF2_HUMAN

UniProt

Q12905 - ILF2_HUMAN

Protein

Interleukin enhancer-binding factor 2

Gene

ILF2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (12 Apr 2005)
      Previous versions | rss
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    Functioni

    Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. Essential for the efficient reshuttling of ILF3 (isoform 1 and isoform 2) into the nucleus.4 Publications

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. DNA binding Source: UniProtKB
    3. double-stranded RNA binding Source: UniProtKB
    4. poly(A) RNA binding Source: UniProtKB
    5. protein binding Source: UniProtKB
    6. transferase activity Source: InterPro

    GO - Biological processi

    1. immune response Source: InterPro
    2. positive regulation of transcription, DNA-templated Source: UniProtKB
    3. transcription, DNA-templated Source: UniProtKB

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Interleukin enhancer-binding factor 2
    Alternative name(s):
    Nuclear factor of activated T-cells 45 kDa
    Gene namesi
    Name:ILF2
    Synonyms:NF45
    ORF Names:PRO3063
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:6037. ILF2.

    Subcellular locationi

    Nucleusnucleolus. Cytoplasm. Nucleus
    Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB
    3. nucleolus Source: UniProtKB
    4. nucleus Source: UniProtKB
    5. ribonucleoprotein complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29852.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 390390Interleukin enhancer-binding factor 2PRO_0000126063Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei388 – 3881Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ12905.
    PaxDbiQ12905.
    PRIDEiQ12905.

    2D gel databases

    SWISS-2DPAGEQ12905.

    PTM databases

    PhosphoSiteiQ12905.

    Expressioni

    Gene expression databases

    ArrayExpressiQ12905.
    BgeeiQ12905.
    CleanExiHS_ILF2.
    GenevestigatoriQ12905.

    Organism-specific databases

    HPAiHPA007484.

    Interactioni

    Subunit structurei

    Forms heterodimers with ILF3. ILF2-ILF3 heterodimers may also bind to PRKDC/XRCC7: this may stabilize the interaction of PRKDC/XRCC7 and the heterodimeric complex of G22P1/KU70 and XRCC5/KU80. Forms a complex with ILF3, YLPM1, KHDRBS1, RBMX, NCOA5 and PPP1CA. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with IGF2BP1.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PPIDQ087524EBI-357925,EBI-716596

    Protein-protein interaction databases

    BioGridi109821. 191 interactions.
    IntActiQ12905. 61 interactions.
    MINTiMINT-4999942.
    STRINGi9606.ENSP00000355011.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12905.
    SMRiQ12905. Positions 29-361.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 371348DZFAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi366 – 3727Poly-Glu
    Compositional biasi381 – 3844Poly-Glu

    Sequence similaritiesi

    Contains 1 DZF domain.Curated

    Phylogenomic databases

    eggNOGiNOG247512.
    HOVERGENiHBG052120.
    InParanoidiQ12905.
    KOiK13089.
    OMAiFEENAHH.
    OrthoDBiEOG75XGM4.
    PhylomeDBiQ12905.
    TreeFamiTF320194.

    Family and domain databases

    InterProiIPR006116. 2-5-oligoadenylate_synth_N.
    IPR006561. DZF.
    [Graphical view]
    PfamiPF07528. DZF. 1 hit.
    [Graphical view]
    SMARTiSM00572. DZF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12905-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRGDRGRGRG GRFGSRGGPG GGFRPFVPHI PFDFYLCEMA FPRVKPAPDE    50
    TSFSEALLKR NQDLAPNSAE QASILSLVTK INNVIDNLIV APGTFEVQIE 100
    EVRQVGSYKK GTMTTGHNVA DLVVILKILP TLEAVAALGN KVVESLRAQD 150
    PSEVLTMLTN ETGFEISSSD ATVKILITTV PPNLRKLDPE LHLDIKVLQS 200
    ALAAIRHARW FEENASQSTV KVLIRLLKDL RIRFPGFEPL TPWILDLLGH 250
    YAVMNNPTRQ PLALNVAYRR CLQILAAGLF LPGSVGITDP CESGNFRVHT 300
    VMTLEQQDMV CYTAQTLVRI LSHGGFRKIL GQEGDASYLA SEISTWDGVI 350
    VTPSEKAYEK PPEKKEGEEE EENTEEPPQG EEEESMETQE 390
    Length:390
    Mass (Da):43,062
    Last modified:April 12, 2005 - v2
    Checksum:i75BAD022DCD4EE01
    GO

    Sequence cautioni

    The sequence AAF29591.1 differs from that shown. Reason: Chimeric cDNA.
    The sequence AAA20993.1 differs from that shown. Reason: Frameshift at position 376.
    The sequence CAI13611.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10323 mRNA. Translation: AAA20993.1. Frameshift.
    AY099265 Genomic DNA. Translation: AAM45141.1.
    AK313364 mRNA. Translation: BAG36164.1.
    AL713889 Genomic DNA. Translation: CAI13610.1.
    AL713889 Genomic DNA. Translation: CAI13611.1. Sequence problems.
    AL713889, AL592150 Genomic DNA. Translation: CAI13612.1.
    AL592150, AL713889 Genomic DNA. Translation: CAI18796.1.
    CH471121 Genomic DNA. Translation: EAW53286.1.
    BC000382 mRNA. Translation: AAH00382.1.
    AF113702 mRNA. Translation: AAF29591.1. Sequence problems.
    CCDSiCCDS1050.1.
    PIRiA54857.
    RefSeqiNP_001254738.1. NM_001267809.1.
    NP_004506.2. NM_004515.3.
    UniGeneiHs.75117.

    Genome annotation databases

    EnsembliENST00000361891; ENSP00000355011; ENSG00000143621.
    GeneIDi3608.
    KEGGihsa:3608.
    UCSCiuc001fcr.4. human.

    Polymorphism databases

    DMDMi62510764.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10323 mRNA. Translation: AAA20993.1 . Frameshift.
    AY099265 Genomic DNA. Translation: AAM45141.1 .
    AK313364 mRNA. Translation: BAG36164.1 .
    AL713889 Genomic DNA. Translation: CAI13610.1 .
    AL713889 Genomic DNA. Translation: CAI13611.1 . Sequence problems.
    AL713889 , AL592150 Genomic DNA. Translation: CAI13612.1 .
    AL592150 , AL713889 Genomic DNA. Translation: CAI18796.1 .
    CH471121 Genomic DNA. Translation: EAW53286.1 .
    BC000382 mRNA. Translation: AAH00382.1 .
    AF113702 mRNA. Translation: AAF29591.1 . Sequence problems.
    CCDSi CCDS1050.1.
    PIRi A54857.
    RefSeqi NP_001254738.1. NM_001267809.1.
    NP_004506.2. NM_004515.3.
    UniGenei Hs.75117.

    3D structure databases

    ProteinModelPortali Q12905.
    SMRi Q12905. Positions 29-361.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109821. 191 interactions.
    IntActi Q12905. 61 interactions.
    MINTi MINT-4999942.
    STRINGi 9606.ENSP00000355011.

    PTM databases

    PhosphoSitei Q12905.

    Polymorphism databases

    DMDMi 62510764.

    2D gel databases

    SWISS-2DPAGE Q12905.

    Proteomic databases

    MaxQBi Q12905.
    PaxDbi Q12905.
    PRIDEi Q12905.

    Protocols and materials databases

    DNASUi 3608.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000361891 ; ENSP00000355011 ; ENSG00000143621 .
    GeneIDi 3608.
    KEGGi hsa:3608.
    UCSCi uc001fcr.4. human.

    Organism-specific databases

    CTDi 3608.
    GeneCardsi GC01M153634.
    HGNCi HGNC:6037. ILF2.
    HPAi HPA007484.
    MIMi 603181. gene.
    neXtProti NX_Q12905.
    PharmGKBi PA29852.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG247512.
    HOVERGENi HBG052120.
    InParanoidi Q12905.
    KOi K13089.
    OMAi FEENAHH.
    OrthoDBi EOG75XGM4.
    PhylomeDBi Q12905.
    TreeFami TF320194.

    Miscellaneous databases

    ChiTaRSi ILF2. human.
    GeneWikii ILF2.
    GenomeRNAii 3608.
    NextBioi 14105.
    PROi Q12905.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q12905.
    Bgeei Q12905.
    CleanExi HS_ILF2.
    Genevestigatori Q12905.

    Family and domain databases

    InterProi IPR006116. 2-5-oligoadenylate_synth_N.
    IPR006561. DZF.
    [Graphical view ]
    Pfami PF07528. DZF. 1 hit.
    [Graphical view ]
    SMARTi SM00572. DZF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of cyclosporin A- and FK506-sensitive nuclear factor of activated T-cells: NF45 and NF90."
      Kao P.N., Chen L., Brock G., Ng J., Kenny J., Smith A.J., Corthesy B.
      J. Biol. Chem. 269:20691-20699(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 62-75; 82-102 AND 330-343, SUBCELLULAR LOCATION.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Thymus.
    3. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.
    6. "Functional prediction of the coding sequences of 32 new genes deduced by analysis of cDNA clones from human fetal liver."
      Zhang C., Yu Y., Zhang S., Ouyang S., Luo L., Wei H., Zhou G., Zhou W., Bi J., Zhang Y., Liu M., He F.
      Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 157-390.
      Tissue: Fetal liver.
    7. "DNA-dependent protein kinase interacts with antigen receptor response element binding proteins NF90 and NF45."
      Ting N.S.Y., Kao P.N., Chan D.W., Lintott L.G., Lees-Miller S.P.
      J. Biol. Chem. 273:2136-2145(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH G22P1; PRKDC AND XRCC5.
    8. "Autoantibodies define a family of proteins with conserved double-stranded RNA-binding domains as well as DNA binding activity."
      Satoh M., Shaheen V.M., Kao P.N., Okano T., Shaw M., Yoshida H., Richards H.B., Reeves W.H.
      J. Biol. Chem. 274:34598-34604(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ILF3.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
    10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. "The RNA binding protein nuclear factor 90 functions as both a positive and negative regulator of gene expression in mammalian cells."
      Reichman T.W., Muniz L.C., Mathews M.B.
      Mol. Cell. Biol. 22:343-356(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ILF3.
    12. "The nuclear PP1 interacting protein ZAP3 (ZAP) is a putative nucleoside kinase that complexes with SAM68, CIA, NF110/45, and HNRNP-G."
      Ulke-Lemee A., Trinkle-Mulcahy L., Chaulk S., Bernstein N.K., Morrice N., Glover M., Lamond A.I., Moorhead G.B.G.
      Biochim. Biophys. Acta 1774:1339-1350(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COMPLEX WITH ILF3; YLPM1; KHDRBS1; RBMX; NCOA5 AND PPP1CA.
    13. Cited for: IDENTIFICATION IN A MRNP GRANULE COMPLEX, INTERACTION WITH IGF2BP1, SUBCELLULAR LOCATION.
    14. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-388, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic kidney.
    15. "Phosphorylation of the NFAR proteins by the dsRNA-dependent protein kinase PKR constitutes a novel mechanism of translational regulation and cellular defense."
      Harashima A., Guettouche T., Barber G.N.
      Genes Dev. 24:2640-2653(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    16. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiILF2_HUMAN
    AccessioniPrimary (citable) accession number: Q12905
    Secondary accession number(s): A6NDB0
    , B2R8G7, Q5SR10, Q5SR11, Q7L7R3, Q9BWD4, Q9P1N0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 12, 2005
    Last sequence update: April 12, 2005
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3