Multisynthetase complex auxiliary component p43

Names and origin

Protein names

Recommended name:
    Multisynthetase complex auxiliary component p43
Cleaved into the following chain:
    1- Recommended name:
            Endothelial monocyte-activating polypeptide 2
        Alternative name(s):
            EMAP-II
            Small inducible cytokine subfamily E member 1

Gene names

Name:	SCYE1
Synonyms:	EMAP2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Seems to have both an inflammatory cytokine activity and tRNA-binding domain. Ref.7
Subunit structure	Monomer. Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43.
Sequence similarities	Contains 1 tRNA-binding domain.

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Ontologies

Keywords
Biological process	Protein biosynthesis
Coding sequence diversity	Polymorphism
Ligand	RNA-binding tRNA-binding
Molecular function	Cytokine
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cell-cell signaling Inferred from direct assay. Source: HGNC chemotaxis Traceable author statement. Source: ProtInc inflammatory response Traceable author statement. Source: ProtInc leukocyte migration Inferred from direct assay. Source: HGNC negative regulation of endothelial cell proliferation Inferred from direct assay. Source: HGNC signal transduction Non-traceable author statement. Source: ProtInc tRNA aminoacylation for protein translation Traceable author statement. Source: ProtInc
Cellular component	aminoacyl-tRNA synthetase multienzyme complex Inferred from direct assay. Source: HGNC extracellular space Traceable author statement. Source: ProtInc
Molecular function	cell surface binding Inferred from direct assay. Source: HGNC cytokine activity Inferred from sequence or structural similarity. Source: HGNC protein homodimerization activity Inferred from direct assay. Source: HGNC tRNA binding Inferred from direct assay. Source: HGNC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 312

312

Multisynthetase complex auxiliary component p43

PRO_0000223394

Chain

147 – 312

166

Endothelial monocyte-activating polypeptide 2

PRO_0000019242

Regions

Domain

151 – 252

102

tRNA-binding

Natural variations

Natural variant

79

1

P → A: dbSNP rs1134648. Ref.1

VAR_025212

Natural variant

104

1

T → A: dbSNP rs2230254.

VAR_029156

Natural variant

117

1

T → A: dbSNP rs2230255.

VAR_050124

Secondary structure

1

.

312

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

Q12904-1 [UniParc].

Last modified January 24, 2006. Version 2.
Checksum: 5F0BF73E58810C60
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FASTA

312

34,353

        10         20         30         40         50         60 
MANNDAVLKR LEQKGAEADQ IIEYLKQQVS LLKEKAILQA TLREEKKLRV ENAKLKKEIE 

        70         80         90        100        110        120 
ELKQELIQAE IQNGVKQIPF PSGTPLHANS MVSENVIQST AVTTVSSGTK EQIKGGTGDE 

       130        140        150        160        170        180 
KKAKEKIEKK GEKKEKKQQS IAGSADSKPI DVSRLDLRIG CIITARKHPD ADSLYVEEVD 

       190        200        210        220        230        240 
VGEIAPRTVV SGLVNHVPLE QMQNRMVILL CNLKPAKMRG VLSQAMVMCA SSPEKIEILA 

       250        260        270        280        290        300 
PPNGSVPGDR ITFDAFPGEP DKELNPKKKI WEQIQPDLHT NDECVATYKG VPFEVKGKGV 

       310 
CRAQTMSNSG IK

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a novel tumor-derived cytokine. Endothelial-monocyte activating polypeptide II." Kao J., Houck K., Fan Y., Haehnel I., Libutti S.K., Kayton M.L., Grikscheit T., Chabot J., Nowygrod R., Greenberg S., Kuang W.J., Leung D.W., Hayward J.R., Kisiel W., Heath M., Brett J., Stern D.M. J. Biol. Chem. 269:25106-25119(1994) [PubMed: 7929199] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ALA-79.
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
[5]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[6]	"A novel anti-tumor cytokine contains an RNA binding motif present in aminoacyl-tRNA synthetases." Kim Y., Shin J., Li R., Cheong C., Kim K., Kim S. J. Biol. Chem. 275:27062-27068(2000) [PubMed: 10852899] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 147-312.
[7]	"Structure of the EMAPII domain of human aminoacyl-tRNA synthetase complex reveals evolutionary dimer mimicry." Renault L., Kerjan P., Pasqualato S., Menetrey J., Robinson J.-C., Kawaguchi S., Vassylyev D.G., Yokoyama S., Mirande M., Cherfils J. EMBO J. 20:570-578(2001) [PubMed: 11157763] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 148-312, FUNCTION.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

U10117 mRNA. Translation: AAA62202.1.
CR542281 mRNA. Translation: CAG47076.1.
AK095951 mRNA. Translation: BAG53174.1.
BC014051 mRNA. Translation: AAH14051.1.

IPI

IPI00006252.

PIR

B55053.

RefSeq

NP_001135887.1.
NP_004748.2.

UniGene

Hs.591680

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1E7Z	X-ray	2.05	A	148-312	[»]
1EUJ	X-ray	1.80	A/B	147-312	[»]
1FL0	X-ray	1.50	A	150-312	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q12904. 5 interactions.

Proteomic databases

PRIDE

Q12904.

Genome annotation databases

Ensembl

ENSG00000164022. Homo sapiens. [Contig view]

GeneID

9255.

KEGG

hsa:9255.

UCSC

uc003hyg.1. human.

Organism-specific databases

GeneCards

GC04P107456.

H-InvDB

HIX0004426.

HGNC

HGNC:10648. SCYE1.

HPA

CAB017618.
HPA018476.

MIM

603605. gene.

PharmGKB

PA35578.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q12904.

HOVERGEN

Q12904.

Enzyme and pathway databases

Reactome

REACT_71. Gene Expression.

Gene expression databases

ArrayExpress

Q12904.

Bgee

Q12904.

CleanEx

HS_SCYE1.

GermOnline

ENSG00000164022. Homo sapiens.

Family and domain databases

InterPro

IPR012340. NA-bd_OB-fold.
IPR002547. tRNA_bd.
[Graphical view]

Gene3D

G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.

Pfam

PF01588. tRNA_bind. 1 hit.
[Graphical view]

PROSITE

PS50886. TRBD. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

34695.

SOURCE

Search...

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Entry information

Entry name

MCA1_HUMAN

Accession

Primary (citable) accession number: Q12904
Secondary accession number(s): B3KTR2, Q6FG28, Q96CQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	January 24, 2006
Last modified:	July 7, 2009
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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