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Protein

Splicing factor 3A subunit 3

Gene

SF3A3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri406 – 437Matrin-typePROSITE-ProRule annotationAdd BLAST32

GO - Molecular functioni

GO - Biological processi

  • mRNA 3'-splice site recognition Source: HGNC
  • mRNA processing Source: HGNC
  • mRNA splicing, via spliceosome Source: Reactome
  • RNA splicing Source: ProtInc
  • RNA splicing, via transesterification reactions Source: UniProtKB

Keywordsi

Biological processmRNA processing, mRNA splicing
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 3A subunit 3
Alternative name(s):
SF3a60
Spliceosome-associated protein 61
Short name:
SAP 61
Gene namesi
Name:SF3A3
Synonyms:SAP61
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000183431.11
HGNCiHGNC:10767 SF3A3
MIMi605596 gene
neXtProtiNX_Q12874

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi10946
OpenTargetsiENSG00000183431
PharmGKBiPA35685

Polymorphism and mutation databases

BioMutaiSF3A3
DMDMi17380310

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001743181 – 501Splicing factor 3A subunit 3Add BLAST501

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1
Modified residuei54PhosphoserineCombined sources1
Glycosylationi119N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei121PhosphoserineCombined sources1
Modified residuei295PhosphoserineCombined sources1
Modified residuei299PhosphoserineCombined sources1
Modified residuei365PhosphoserineCombined sources1
Modified residuei367PhosphoserineCombined sources1
Modified residuei369PhosphoserineCombined sources1
Modified residuei475PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ12874
MaxQBiQ12874
PaxDbiQ12874
PeptideAtlasiQ12874
PRIDEiQ12874

PTM databases

iPTMnetiQ12874
PhosphoSitePlusiQ12874
SwissPalmiQ12874

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000183431
CleanExiHS_SF3A3
GenevisibleiQ12874 HS

Organism-specific databases

HPAiHPA032054
HPA032055

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interacts with SF3A1, through its N-terminal region.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi116146, 135 interactors
CORUMiQ12874
DIPiDIP-882N
IntActiQ12874, 27 interactors
MINTiQ12874
STRINGi9606.ENSP00000362110

Structurei

Secondary structure

1501
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi80 – 96Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DT7NMR-A71-107[»]
ProteinModelPortaliQ12874
SMRiQ12874
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12874

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi354 – 362Poly-Glu9

Sequence similaritiesi

Belongs to the SF3A3 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri406 – 437Matrin-typePROSITE-ProRule annotationAdd BLAST32

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2636 Eukaryota
COG5188 LUCA
GeneTreeiENSGT00530000063402
HOGENOMiHOG000160935
HOVERGENiHBG054452
InParanoidiQ12874
KOiK12827
OMAiEFPCEIC
OrthoDBiEOG091G059G
PhylomeDBiQ12874
TreeFamiTF315227

Family and domain databases

InterProiView protein in InterPro
IPR024598 DUF3449
IPR000690 Matrin/U1-C_Znf_C2H2
IPR031776 SF3A3
IPR031774 SF3A3_dom
IPR021966 SF3a60_bindingd
PANTHERiPTHR12786:SF2 PTHR12786:SF2, 1 hit
PfamiView protein in Pfam
PF11931 DUF3449, 1 hit
PF16837 SF3A3, 1 hit
PF12108 SF3a60_bindingd, 1 hit
PROSITEiView protein in PROSITE
PS50171 ZF_MATRIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q12874-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METILEQQRR YHEEKERLMD VMAKEMLTKK STLRDQINSD HRTRAMQDRY
60 70 80 90 100
MEVSGNLRDL YDDKDGLRKE ELNAISGPNE FAEFYNRLKQ IKEFHRKHPN
110 120 130 140 150
EICVPMSVEF EELLKARENP SEEAQNLVEF TDEEGYGRYL DLHDCYLKYI
160 170 180 190 200
NLKASEKLDY ITYLSIFDQL FDIPKERKNA EYKRYLEMLL EYLQDYTDRV
210 220 230 240 250
KPLQDQNELF GKIQAEFEKK WENGTFPGWP KETSSALTHA GAHLDLSAFS
260 270 280 290 300
SWEELASLGL DRLKSALLAL GLKCGGTLEE RAQRLFSTKG KSLESLDTSL
310 320 330 340 350
FAKNPKSKGT KRDTERNKDI AFLEAQIYEY VEILGEQRHL THENVQRKQA
360 370 380 390 400
RTGEEREEEE EEQISESESE DEENEIIYNP KNLPLGWDGK PIPYWLYKLH
410 420 430 440 450
GLNINYNCEI CGNYTYRGPK AFQRHFAEWR HAHGMRCLGI PNTAHFANVT
460 470 480 490 500
QIEDAVSLWA KLKLQKASER WQPDTEEEYE DSSGNVVNKK TYEDLKRQGL

L
Length:501
Mass (Da):58,849
Last modified:November 1, 1996 - v1
Checksum:i6E6F6EA17777E1E2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti176E → G in CAA57388 (PubMed:7816610).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08815 mRNA Translation: AAA19625.1
X81789 mRNA Translation: CAA57388.1
AL603790 Genomic DNA No translation available.
CH471059 Genomic DNA Translation: EAX07304.1
CH471059 Genomic DNA Translation: EAX07305.1
BC002395 mRNA Translation: AAH02395.1
BC011523 mRNA Translation: AAH11523.1
CCDSiCCDS428.1
PIRiA55749
RefSeqiNP_006793.1, NM_006802.3
UniGeneiHs.77897

Genome annotation databases

EnsembliENST00000373019; ENSP00000362110; ENSG00000183431
GeneIDi10946
KEGGihsa:10946
UCSCiuc001cci.4 human

Similar proteinsi

Entry informationi

Entry nameiSF3A3_HUMAN
AccessioniPrimary (citable) accession number: Q12874
Secondary accession number(s): D3DPT5, Q15460, Q5VT87
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 184 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families
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