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Protein

Splicing factor 3A subunit 3

Gene

SF3A3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri406 – 437Matrin-typePROSITE-ProRule annotationAdd BLAST32

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • RNA binding Source: GO_Central
  • zinc ion binding Source: InterPro

GO - Biological processi

  • mRNA 3'-splice site recognition Source: HGNC
  • mRNA processing Source: HGNC
  • mRNA splicing, via spliceosome Source: UniProtKB
  • RNA splicing Source: ProtInc
  • RNA splicing, via transesterification reactions Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:G66-33362-MONOMER.
ReactomeiR-HSA-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 3A subunit 3
Alternative name(s):
SF3a60
Spliceosome-associated protein 61
Short name:
SAP 61
Gene namesi
Name:SF3A3
Synonyms:SAP61
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:10767. SF3A3.

Subcellular locationi

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: UniProtKB
  • nuclear speck Source: UniProtKB-SubCell
  • nucleoplasm Source: HPA
  • spliceosomal complex Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi10946.
OpenTargetsiENSG00000183431.
PharmGKBiPA35685.

Polymorphism and mutation databases

BioMutaiSF3A3.
DMDMi17380310.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001743181 – 501Splicing factor 3A subunit 3Add BLAST501

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1
Modified residuei54PhosphoserineCombined sources1
Glycosylationi119N-linked (GlcNAc...)1 Publication1
Modified residuei121PhosphoserineCombined sources1
Modified residuei295PhosphoserineCombined sources1
Modified residuei299PhosphoserineCombined sources1
Modified residuei365PhosphoserineCombined sources1
Modified residuei367PhosphoserineCombined sources1
Modified residuei369PhosphoserineCombined sources1
Modified residuei475PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ12874.
MaxQBiQ12874.
PaxDbiQ12874.
PeptideAtlasiQ12874.
PRIDEiQ12874.

PTM databases

iPTMnetiQ12874.
PhosphoSitePlusiQ12874.
SwissPalmiQ12874.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000183431.
CleanExiHS_SF3A3.
GenevisibleiQ12874. HS.

Organism-specific databases

HPAiHPA032054.
HPA032055.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interacts with SF3A1, through its N-terminal region.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HSPB1P047922EBI-1051880,EBI-352682
ORFQ9Q2G43EBI-1051880,EBI-6248094From a different organism.
SF3A1Q154592EBI-1051880,EBI-1054743
TADA2AO754783EBI-1051880,EBI-742268
TRIM69Q86WT63EBI-1051880,EBI-749955
ZNF212Q9UDV63EBI-1051880,EBI-1640204

Protein-protein interaction databases

BioGridi116146. 122 interactors.
DIPiDIP-882N.
IntActiQ12874. 26 interactors.
MINTiMINT-1467744.
STRINGi9606.ENSP00000362110.

Structurei

Secondary structure

1501
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi80 – 96Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DT7NMR-A71-107[»]
ProteinModelPortaliQ12874.
SMRiQ12874.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12874.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi354 – 362Poly-Glu9

Sequence similaritiesi

Belongs to the SF3A3 family.Curated
Contains 1 matrin-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri406 – 437Matrin-typePROSITE-ProRule annotationAdd BLAST32

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2636. Eukaryota.
COG5188. LUCA.
GeneTreeiENSGT00530000063402.
HOGENOMiHOG000160935.
HOVERGENiHBG054452.
InParanoidiQ12874.
KOiK12827.
OMAiPKKDTER.
OrthoDBiEOG091G059G.
PhylomeDBiQ12874.
TreeFamiTF315227.

Family and domain databases

InterProiIPR024598. DUF3449.
IPR031776. SF3A3.
IPR031774. SF3A3_dom.
IPR021966. SF3a60_bindingd.
IPR000690. Znf_C2H2_matrin.
[Graphical view]
PANTHERiPTHR12786:SF2. PTHR12786:SF2. 1 hit.
PfamiPF11931. DUF3449. 1 hit.
PF16837. SF3A3. 1 hit.
PF12108. SF3a60_bindingd. 1 hit.
[Graphical view]
PROSITEiPS50171. ZF_MATRIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12874-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METILEQQRR YHEEKERLMD VMAKEMLTKK STLRDQINSD HRTRAMQDRY
60 70 80 90 100
MEVSGNLRDL YDDKDGLRKE ELNAISGPNE FAEFYNRLKQ IKEFHRKHPN
110 120 130 140 150
EICVPMSVEF EELLKARENP SEEAQNLVEF TDEEGYGRYL DLHDCYLKYI
160 170 180 190 200
NLKASEKLDY ITYLSIFDQL FDIPKERKNA EYKRYLEMLL EYLQDYTDRV
210 220 230 240 250
KPLQDQNELF GKIQAEFEKK WENGTFPGWP KETSSALTHA GAHLDLSAFS
260 270 280 290 300
SWEELASLGL DRLKSALLAL GLKCGGTLEE RAQRLFSTKG KSLESLDTSL
310 320 330 340 350
FAKNPKSKGT KRDTERNKDI AFLEAQIYEY VEILGEQRHL THENVQRKQA
360 370 380 390 400
RTGEEREEEE EEQISESESE DEENEIIYNP KNLPLGWDGK PIPYWLYKLH
410 420 430 440 450
GLNINYNCEI CGNYTYRGPK AFQRHFAEWR HAHGMRCLGI PNTAHFANVT
460 470 480 490 500
QIEDAVSLWA KLKLQKASER WQPDTEEEYE DSSGNVVNKK TYEDLKRQGL

L
Length:501
Mass (Da):58,849
Last modified:November 1, 1996 - v1
Checksum:i6E6F6EA17777E1E2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti176E → G in CAA57388 (PubMed:7816610).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08815 mRNA. Translation: AAA19625.1.
X81789 mRNA. Translation: CAA57388.1.
AL603790 Genomic DNA. Translation: CAH69930.1.
CH471059 Genomic DNA. Translation: EAX07304.1.
CH471059 Genomic DNA. Translation: EAX07305.1.
BC002395 mRNA. Translation: AAH02395.1.
BC011523 mRNA. Translation: AAH11523.1.
CCDSiCCDS428.1.
PIRiA55749.
RefSeqiNP_006793.1. NM_006802.3.
UniGeneiHs.77897.

Genome annotation databases

EnsembliENST00000373019; ENSP00000362110; ENSG00000183431.
GeneIDi10946.
KEGGihsa:10946.
UCSCiuc001cci.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08815 mRNA. Translation: AAA19625.1.
X81789 mRNA. Translation: CAA57388.1.
AL603790 Genomic DNA. Translation: CAH69930.1.
CH471059 Genomic DNA. Translation: EAX07304.1.
CH471059 Genomic DNA. Translation: EAX07305.1.
BC002395 mRNA. Translation: AAH02395.1.
BC011523 mRNA. Translation: AAH11523.1.
CCDSiCCDS428.1.
PIRiA55749.
RefSeqiNP_006793.1. NM_006802.3.
UniGeneiHs.77897.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DT7NMR-A71-107[»]
ProteinModelPortaliQ12874.
SMRiQ12874.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116146. 122 interactors.
DIPiDIP-882N.
IntActiQ12874. 26 interactors.
MINTiMINT-1467744.
STRINGi9606.ENSP00000362110.

PTM databases

iPTMnetiQ12874.
PhosphoSitePlusiQ12874.
SwissPalmiQ12874.

Polymorphism and mutation databases

BioMutaiSF3A3.
DMDMi17380310.

Proteomic databases

EPDiQ12874.
MaxQBiQ12874.
PaxDbiQ12874.
PeptideAtlasiQ12874.
PRIDEiQ12874.

Protocols and materials databases

DNASUi10946.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373019; ENSP00000362110; ENSG00000183431.
GeneIDi10946.
KEGGihsa:10946.
UCSCiuc001cci.4. human.

Organism-specific databases

CTDi10946.
DisGeNETi10946.
GeneCardsiSF3A3.
HGNCiHGNC:10767. SF3A3.
HPAiHPA032054.
HPA032055.
MIMi605596. gene.
neXtProtiNX_Q12874.
OpenTargetsiENSG00000183431.
PharmGKBiPA35685.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2636. Eukaryota.
COG5188. LUCA.
GeneTreeiENSGT00530000063402.
HOGENOMiHOG000160935.
HOVERGENiHBG054452.
InParanoidiQ12874.
KOiK12827.
OMAiPKKDTER.
OrthoDBiEOG091G059G.
PhylomeDBiQ12874.
TreeFamiTF315227.

Enzyme and pathway databases

BioCyciZFISH:G66-33362-MONOMER.
ReactomeiR-HSA-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSiSF3A3. human.
EvolutionaryTraceiQ12874.
GeneWikiiSF3A3.
GenomeRNAii10946.
PROiQ12874.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000183431.
CleanExiHS_SF3A3.
GenevisibleiQ12874. HS.

Family and domain databases

InterProiIPR024598. DUF3449.
IPR031776. SF3A3.
IPR031774. SF3A3_dom.
IPR021966. SF3a60_bindingd.
IPR000690. Znf_C2H2_matrin.
[Graphical view]
PANTHERiPTHR12786:SF2. PTHR12786:SF2. 1 hit.
PfamiPF11931. DUF3449. 1 hit.
PF16837. SF3A3. 1 hit.
PF12108. SF3a60_bindingd. 1 hit.
[Graphical view]
PROSITEiPS50171. ZF_MATRIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSF3A3_HUMAN
AccessioniPrimary (citable) accession number: Q12874
Secondary accession number(s): D3DPT5, Q15460, Q5VT87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 171 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.