Q12857 (NFIA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear factor 1 A-type
Short name=NF1-A
Short name=Nuclear factor 1/A

Alternative name(s):
CCAAT-box-binding transcription factor
Short name=CTF
Nuclear factor I/A
Short name=NF-I/A
Short name=NFI-A
TGGCA-binding protein

Gene names

Name:	NFIA
Synonyms:	KIAA1439

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Subunit structure	Binds DNA as a homodimer.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the CTF/NF-I family. Contains 1 CTF/NF-I DNA-binding domain.
Sequence caution	The sequence BAA92677.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	DNA replication Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Non-traceable author statement Ref.5. Source: UniProtKB
Cellular_component	cell junction Inferred from direct assay. Source: HPA nucleus Inferred from direct assay PubMed 15684392. Source: UniProtKB
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Non-traceable author statement Ref.5. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q12857-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q12857-2)

The sequence of this isoform differs from the canonical sequence as follows:
     474-509: TYSTPSTSPANRFVSVGPRDPSFVNIPQQTQSWYLG → ILVPGIKVAASHHPPDRPPDPFSTL

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	Nuclear factor 1 A-type		PRO_0000100191
Regions
	DNA binding	1 – 194	194	CTF/NF-I
Amino acid modifications
	Modified residue	258	1	Phosphoserine Ref.8
	Modified residue	265	1	Phosphoserine Ref.8 Ref.10
	Modified residue	280	1	Phosphoserine Ref.8 Ref.9 Ref.10
	Modified residue	287	1	Phosphoserine Ref.7 Ref.8 Ref.9 Ref.10
	Modified residue	300	1	Phosphoserine Ref.8 Ref.10
	Modified residue	319	1	Phosphoserine Ref.8
	Modified residue	360	1	Phosphoserine Ref.10
Natural variations
	Alternative sequence	474 – 509	36	TYSTP…SWYLG → ILVPGIKVAASHHPPDRPPD PFSTL in isoform 2.		VSP_036620
Experimental info
	Sequence conflict	186	1	A → G in AAA93124. Ref.5
	Sequence conflict	240 – 243	4	TGPN → PAPT in AAA93124. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 2. Checksum: 42090C6B8B229F87	FASTA	509	55,944
	10 20 30 40 50 60 MYSPLCLTQD EFHPFIEALL PHVRAFAYTW FNLQARKRKY FKKHEKRMSK EEERAVKDEL 70 80 90 100 110 120 LSEKPEVKQK WASRLLAKLR KDIRPEYRED FVLTVTGKKP PCCVLSNPDQ KGKMRRIDCL 130 140 150 160 170 180 RQADKVWRLD LVMVILFKGI PLESTDGERL VKSPQCSNPG LCVQPHHIGV SVKELDLYLA 190 200 210 220 230 240 YFVHAADSSQ SESPSQPSDA DIKDQPENGH LGFQDSFVTS GVFSVTELVR VSQTPIAAGT 250 260 270 280 290 300 GPNFSLSDLE SSSYYSMSPG AMRRSLPSTS STSSTKRLKS VEDEMDSPGE EPFYTGQGRS 310 320 330 340 350 360 PGSGSQSSGW HEVEPGMPSP TTLKKSEKSG FSSPSPSQTS SLGTAFTQHH RPVITGPRAS 370 380 390 400 410 420 PHATPSTLHF PTSPIIQQPG PYFSHPAIRY HPQETLKEFV QLVCPDAGQQ AGQVGFLNPN 430 440 450 460 470 480 GSSQGKVHNP FLPTPMLPPP PPPPMARPVP LPVPDTKPPT TSTEGGAASP TSPTYSTPST 490 500 SPANRFVSVG PRDPSFVNIP QQTQSWYLG
	Isoform 2 [UniParc]. Checksum: 0C47EA46EC78030C	FASTA	498	54,620
	10 20 30 40 50 60 MYSPLCLTQD EFHPFIEALL PHVRAFAYTW FNLQARKRKY FKKHEKRMSK EEERAVKDEL 70 80 90 100 110 120 LSEKPEVKQK WASRLLAKLR KDIRPEYRED FVLTVTGKKP PCCVLSNPDQ KGKMRRIDCL 130 140 150 160 170 180 RQADKVWRLD LVMVILFKGI PLESTDGERL VKSPQCSNPG LCVQPHHIGV SVKELDLYLA 190 200 210 220 230 240 YFVHAADSSQ SESPSQPSDA DIKDQPENGH LGFQDSFVTS GVFSVTELVR VSQTPIAAGT 250 260 270 280 290 300 GPNFSLSDLE SSSYYSMSPG AMRRSLPSTS STSSTKRLKS VEDEMDSPGE EPFYTGQGRS 310 320 330 340 350 360 PGSGSQSSGW HEVEPGMPSP TTLKKSEKSG FSSPSPSQTS SLGTAFTQHH RPVITGPRAS 370 380 390 400 410 420 PHATPSTLHF PTSPIIQQPG PYFSHPAIRY HPQETLKEFV QLVCPDAGQQ AGQVGFLNPN 430 440 450 460 470 480 GSSQGKVHNP FLPTPMLPPP PPPPMARPVP LPVPDTKPPT TSTEGGAASP TSPILVPGIK 490 VAASHHPPDR PPDPFSTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Skeletal muscle.
[5]	"Chromosomal localization of the four genes (NFIA, B, C, and X) for the human transcription factor nuclear factor I by FISH." Qian F., Kruse U., Lichter P., Sippel A.E. Genomics 28:66-73(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 19-243.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[7]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-287, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-258; SER-265; SER-280; SER-287; SER-300 AND SER-319, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-280 AND SER-287, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-265; SER-280; SER-287; SER-300 AND SER-360, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB037860 mRNA. Translation: BAA92677.1. Different initiation. AL445198, AC096534, AL096888 Genomic DNA. Translation: CAI13080.1. AL445198, AC096534, AL096888 Genomic DNA. Translation: CAI13081.1. AL096888, AC096534, AL445198 Genomic DNA. Translation: CAI23240.1. AL096888, AC096534, AL445198 Genomic DNA. Translation: CAI23241.1. CH471059 Genomic DNA. Translation: EAX06601.1. BC022264 mRNA. Translation: AAH22264.1. U07809 mRNA. Translation: AAA93124.1.
IPI	IPI00029745. IPI00923394.
RefSeq	NP_001128145.1. NM_001134673.3. NP_001138983.1. NM_001145511.1. NP_001138984.1. NM_001145512.1. NP_005586.1. NM_005595.4.
UniGene	Hs.710546. Hs.740757.
3D structure databases
ProteinModelPortal	Q12857.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000384523.
PTM databases
PhosphoSite	Q12857.
Polymorphism databases
DMDM	14194959.
Proteomic databases
PaxDb	Q12857.
PRIDE	Q12857.
Protocols and materials databases
DNASU	4774.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000403491; ENSP00000384523; ENSG00000162599. ENST00000485903; ENSP00000419785; ENSG00000162599.
GeneID	4774.
KEGG	hsa:4774.
UCSC	uc001czv.3. human. uc001czw.3. human.
Organism-specific databases
CTD	4774.
GeneCards	GC01P061260.
HGNC	HGNC:7784. NFIA.
HPA	HPA006111. HPA008884.
MIM	600727. gene.
neXtProt	NX_Q12857.
PharmGKB	PA31590.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG252172.
HOGENOM	HOG000013028.
HOVERGEN	HBG006561.
KO	K09168.
OrthoDB	EOG4ZKJM9.
Enzyme and pathway databases
Pathway_Interaction_DB	hnf3apathway. FOXA1 transcription factor network.
Gene expression databases
ArrayExpress	Q12857.
Bgee	Q12857.
CleanEx	HS_NFIA.
Genevestigator	Q12857.
Family and domain databases
InterPro	IPR000647. CTF/NFI. IPR020604. CTF/NFI_DNA-bd-dom. IPR019739. CTF/NFI_DNA-bd_CS. IPR019548. CTF/NFI_DNA-bd_N. IPR003619. MAD_homology1_Dwarfin-type. [Graphical view]
PANTHER	PTHR11492. PTHR11492. 1 hit.
Pfam	PF00859. CTF_NFI. 1 hit. PF03165. MH1. 1 hit. PF10524. NfI_DNAbd_pre-N. 1 hit. [Graphical view]
SMART	SM00523. DWA. 1 hit. [Graphical view]
PROSITE	PS00349. CTF_NFI_1. 1 hit. PS51080. CTF_NFI_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NFIA. human.
GenomeRNAi	4774.
NextBio	18406.
SOURCE	Search...

Entry information

Entry name

NFIA_HUMAN

Accession

Primary (citable) accession number: Q12857
Secondary accession number(s): Q8TA97, Q9H3X9, Q9P2A9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.