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Reviewed, UniProtKB/Swiss-Prot Q12846 (STX4_HUMAN)

Last modified January 19, 2010. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Syntaxin-4
Alternative name(s):
    Renal carcinoma antigen NY-REN-31
Gene names
Name: STX4
Synonyms: STX4A
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length297 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes By similarity. May also play a role in docking of synaptic vesicles at presynaptic active zones.

Subunit structure

Binds STXBP3 and STXBP6. Binding to STXBP3 excludes binding with SNAP25. Interacts with SNAP23 and SNAP25BP. Detected in a complex with SNAP23 and STXBP4. Found in a complex with VAMP8 and SNAP23 in pancreas By similarity. Interacts with LLGL1, VAMP2, STXBP4 and STX4A By similarity. Interaction with STXBP4 excludes interaction with VAMP2 By similarity. Interacts (via C-terminus) with CENPF By similarity.

Subcellular location

Cell membrane; Single-pass type IV membrane protein Potential.

Tissue specificity

Expressed in neutrophils and neutrophil-differentiated HL-60 cells. Expression in neutrophils increases with differentiation. Ref.3

Sequence similarities

Belongs to the syntaxin family.

Contains 1 t-SNARE coiled-coil homology domain.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

NAPAP549201EBI-744942,EBI-749652

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 297297Syntaxin-4
PRO_0000210202

Regions

Topological domain1 – 275275Cytoplasmic Potential
Transmembrane276 – 29621Anchor for type IV membrane protein Potential
Topological domain2971Extracellular Potential
Domain200 – 26263t-SNARE coiled-coil homology
Region154 – 297144Interaction with CENPF By similarity
Coiled coil43 – 163121 Potential

Amino acid modifications

Modified residue141Phosphoserine Ref.12
Modified residue151Phosphoserine Ref.12
Modified residue1151Phosphotyrosine By similarity
Modified residue1171Phosphoserine Ref.12
Modified residue2511Phosphotyrosine Ref.14

Experimental info

Sequence conflict1741E → D in AAA20967. Ref.1
Sequence conflict2691A → V in AAA20967. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q12846-1 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: 5084FD1C49A86BAA

FASTA29734,180
        10         20         30         40         50         60 
MRDRTHELRQ GDDSSDEEDK ERVALVVHPG TARLGSPDEE FFHKVRTIRQ TIVKLGNKVQ 

        70         80         90        100        110        120 
ELEKQQVTIL ATPLPEESMK QELQNLRDEI KQLGREIRLQ LKAIEPQKEE ADENYNSVNT 

       130        140        150        160        170        180 
RMRKTQHGVL SQQFVELINK CNSMQSEYRE KNVERIRRQL KITNAGMVSD EELEQMLDSG 

       190        200        210        220        230        240 
QSEVFVSNIL KDTQVTRQAL NEISARHSEI QQLERSIREL HDIFTFLATE VEMQGEMINR 

       250        260        270        280        290 
IEKNILSSAD YVERGQEHVK TALENQKKAR KKKVLIAICV SITVVLLAVI IGVTVVG

« Hide

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References

« Hide 'large scale' references
[1]"Isolation and sequence analysis of the human syntaxin-encoding gene."
Li H., Hodge D.R., Pei G.K., Seth A.
Gene 143:303-304(1994) [PubMed: 8206394] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Placenta.
[2]"Insulin-responsive tissues contain the core complex protein SNAP-25 (synaptosomal-associated protein 25) A and B isoforms in addition to syntaxin 4 and synaptobrevins 1 and 2."
Jagadish M.N., Fernandez C.S., Hewish D.R., Macaulay S.L., Gough K.H., Grusovin J., Verkuylen A., Cosgrove L., Alafaci A., Frenkel M.J., Ward C.W.
Biochem. J. 317:945-954(1996) [PubMed: 8760387] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Skeletal muscle.
[3]"Co-expression of several human syntaxin genes in neutrophils and differentiating HL-60 cells: variant isoforms and detection of syntaxin 1."
Martin-Martin B., Nabokina S.M., Lazo P.A., Mollinedo F.
J. Leukoc. Biol. 65:397-406(1999) [PubMed: 10080545] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Peripheral blood neutrophil.
[4]Rae J.L., Shepard A.R.
Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lens epithelium.
[5]"Expression of syntaxins in kidney MDCK cells."
Zhang Z., Loh E., Low S.H., Li X., Miura M., An F., Weimbs T.
Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[7]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[9]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skin.
[11]"Antigens recognized by autologous antibody in patients with renal-cell carcinoma."
Scanlan M.J., Gordan J.D., Williamson B., Stockert E., Bander N.H., Jongeneel C.V., Gure A.O., Jaeger D., Jaeger E., Knuth A., Chen Y.-T., Old L.J.
Int. J. Cancer 83:456-464(1999) [PubMed: 10508479] [Abstract]
Cited for: IDENTIFICATION AS A RENAL CANCER ANTIGEN.
Tissue: Renal cell carcinoma.
[12]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14; SER-15 AND SER-117, MASS SPECTROMETRY.
[13]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[14]"An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells."
Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A., Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D., Wiley H.S., Qian W.-J.
J. Proteome Res. 8:3852-3861(2009) [PubMed: 19534553] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-251, MASS SPECTROMETRY.
Tissue: Mammary epithelium.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U07158 mRNA. Translation: AAA20967.1.
X85784 mRNA. Translation: CAA59769.1.
AJ000541 mRNA. Translation: CAA04174.1.
AF026007 mRNA. Translation: AAB88810.1.
AF318489 Transcribed RNA. Translation: AAG40313.1.
BT007326 mRNA. Translation: AAP35990.1.
CR541806 mRNA. Translation: CAG46605.1.
AK315716 mRNA. Translation: BAG38075.1.
CH471192 Genomic DNA. Translation: EAW52176.1.
BC002436 mRNA. Translation: AAH02436.1.
IPIIPI00029730.
PIRI38517.
S52726.
RefSeqNP_004595.2.
UniGeneHs.83734

3D structure databases

SMRQ12846. Positions 39-234, 200-255.
ModBaseSearch...

Protein-protein interaction databases

IntActQ12846. 5 interactions.
STRINGQ12846.

Protein family/group databases

TCDB1.F.1.1.1. synaptosomal vesicle fusion pore (SVF-Pore) family.

PTM databases

PhosphoSiteQ12846.

2-D gel databases

OGPQ12846.

Proteomic databases

PRIDEQ12846.

Genome annotation databases

EnsemblENST00000313843; ENSP00000317714; ENSG00000103496; Homo sapiens. [Genome view]
GeneID6810.
KEGGhsa:6810.
UCSCuc002eal.1. human.

Organism-specific databases

CTD6810.
GeneCardsGC16P030953.
H-InvDBHIX0017907.
HGNCHGNC:11439. STX4.
HPAHPA001330.
MIM186591. gene.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG19699.
HOGENOMHBG717663.
HOVERGENQ12846.
InParanoidQ12846.
OMAVNTRMRK.
PhylomeDBQ12846.

Enzyme and pathway databases

Pathway_Interaction_DBinsulin_glucose_pathway. Insulin-mediated glucose transport.
ReactomeREACT_11123. Membrane Trafficking.
REACT_11184. Botulinum neurotoxicity.
REACT_604. Hemostasis.

Gene expression databases

ArrayExpressQ12846.
BgeeQ12846.
CleanExHS_STX4.
GenevestigatorQ12846.
GermOnlineENSG00000103496. Homo sapiens.

Family and domain databases

InterProIPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
PROSITEPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio26573.
SOURCESearch...

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Entry information

Entry nameSTX4_HUMAN
AccessionPrimary (citable) accession number: Q12846
Secondary accession number(s): Q15525, Q6FHE8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1998
Last modified: January 19, 2010
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents