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Protein

Syntaxin-4

Gene

STX4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes (By similarity). May also play a role in docking of synaptic vesicles at presynaptic active zones.By similarity

GO - Molecular functioni

  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: GO_Central
  • sphingomyelin phosphodiesterase activator activity Source: UniProtKB

GO - Biological processi

  • cellular response to interferon-gamma Source: Ensembl
  • cytokine-mediated signaling pathway Source: Reactome
  • intracellular protein transport Source: GO_Central
  • long-term synaptic potentiation Source: UniProtKB
  • organelle fusion Source: UniProtKB
  • positive regulation of catalytic activity Source: UniProtKB
  • positive regulation of cell adhesion Source: UniProtKB
  • positive regulation of cell migration Source: UniProtKB
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of chemotaxis Source: UniProtKB
  • positive regulation of eosinophil degranulation Source: UniProtKB
  • positive regulation of immunoglobulin secretion Source: UniProtKB
  • positive regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
  • positive regulation of protein localization to cell surface Source: UniProtKB
  • positive regulation of protein localization to plasma membrane Source: UniProtKB
  • regulation of exocytosis Source: UniProtKB
  • regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
  • response to hydroperoxide Source: UniProtKB
  • SNARE complex assembly Source: Ensembl
  • synaptic vesicle fusion to presynaptic active zone membrane Source: GO_Central
  • vesicle docking Source: GO_Central
  • vesicle fusion with endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membrane Source: Reactome

Keywordsi

Biological processNeurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-114516 Disinhibition of SNARE formation
R-HSA-1236974 ER-Phagosome pathway
R-HSA-1445148 Translocation of GLUT4 to the plasma membrane
R-HSA-421837 Clathrin derived vesicle budding
R-HSA-449836 Other interleukin signaling

Protein family/group databases

TCDBi1.F.1.1.1 the synaptosomal vesicle fusion pore (svf-pore) family

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-4
Alternative name(s):
Renal carcinoma antigen NY-REN-31
Gene namesi
Name:STX4
Synonyms:STX4A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000103496.14
HGNCiHGNC:11439 STX4
MIMi186591 gene
neXtProtiNX_Q12846

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 275CytoplasmicSequence analysisAdd BLAST275
Transmembranei276 – 296Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini297ExtracellularSequence analysis1

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi6810
OpenTargetsiENSG00000103496
PharmGKBiPA36236

Polymorphism and mutation databases

DMDMi3041737

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002102021 – 297Syntaxin-4Add BLAST297

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineCombined sources1
Modified residuei15PhosphoserineCombined sources1
Modified residuei31PhosphothreonineCombined sources1
Modified residuei36PhosphoserineCombined sources1
Modified residuei117PhosphoserineCombined sources1
Modified residuei208PhosphoserineBy similarity1
Modified residuei248PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ12846
MaxQBiQ12846
PaxDbiQ12846
PeptideAtlasiQ12846
PRIDEiQ12846
ProteomicsDBi58981

2D gel databases

OGPiQ12846

PTM databases

iPTMnetiQ12846
PhosphoSitePlusiQ12846
SwissPalmiQ12846

Expressioni

Tissue specificityi

Expressed in neutrophils and neutrophil-differentiated HL-60 cells. Expression in neutrophils increases with differentiation.1 Publication

Gene expression databases

BgeeiENSG00000103496
CleanExiHS_STX4
ExpressionAtlasiQ12846 baseline and differential
GenevisibleiQ12846 HS

Organism-specific databases

HPAiHPA001330

Interactioni

Subunit structurei

Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that interacts with SYT7 during lysosomal exocytosis. Found in a complex with VAMP8 and SNAP23. Detected in a complex with SNAP23 and STXBP4. Interacts with VAMP2. Interacts with SNAP23 and SNAPIN. Interacts with LLGL1. Interacts (via C-terminus) with CENPF. Interacts with DOC2B. Interacts with STXBP6. Interacts with STXBP3; excludes interaction with DOC2B and SNAP25. Interacts with STXBP4; excludes interaction with VAMP2 (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112679, 72 interactors
CORUMiQ12846
IntActiQ12846, 49 interactors
MINTiQ12846
STRINGi9606.ENSP00000317714

Structurei

3D structure databases

ProteinModelPortaliQ12846
SMRiQ12846
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini200 – 262t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni154 – 297Interaction with CENPFBy similarityAdd BLAST144

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili43 – 163Sequence analysisAdd BLAST121

Sequence similaritiesi

Belongs to the syntaxin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0810 Eukaryota
COG5074 LUCA
GeneTreeiENSGT00760000119200
HOGENOMiHOG000286023
HOVERGENiHBG000497
InParanoidiQ12846
KOiK13502
OMAiQYLAMEV
OrthoDBiEOG091G0EUK
PhylomeDBiQ12846
TreeFamiTF313763

Family and domain databases

InterProiView protein in InterPro
IPR010989 SNARE
IPR006012 Syntaxin/epimorphin_CS
IPR006011 Syntaxin_N
IPR000727 T_SNARE_dom
PfamiView protein in Pfam
PF05739 SNARE, 1 hit
PF00804 Syntaxin, 1 hit
SMARTiView protein in SMART
SM00503 SynN, 1 hit
SM00397 t_SNARE, 1 hit
SUPFAMiSSF47661 SSF47661, 1 hit
PROSITEiView protein in PROSITE
PS00914 SYNTAXIN, 1 hit
PS50192 T_SNARE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q12846-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRDRTHELRQ GDDSSDEEDK ERVALVVHPG TARLGSPDEE FFHKVRTIRQ
60 70 80 90 100
TIVKLGNKVQ ELEKQQVTIL ATPLPEESMK QELQNLRDEI KQLGREIRLQ
110 120 130 140 150
LKAIEPQKEE ADENYNSVNT RMRKTQHGVL SQQFVELINK CNSMQSEYRE
160 170 180 190 200
KNVERIRRQL KITNAGMVSD EELEQMLDSG QSEVFVSNIL KDTQVTRQAL
210 220 230 240 250
NEISARHSEI QQLERSIREL HDIFTFLATE VEMQGEMINR IEKNILSSAD
260 270 280 290
YVERGQEHVK TALENQKKAR KKKVLIAICV SITVVLLAVI IGVTVVG
Length:297
Mass (Da):34,180
Last modified:July 15, 1998 - v2
Checksum:i5084FD1C49A86BAA
GO
Isoform 2 (identifier: Q12846-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: MRDRTHELRQ...GSPDEEFFHK → MGMTARTKRT...STSPLGHPPQ

Note: No experimental confirmation available.
Show »
Length:295
Mass (Da):33,841
Checksum:i0E0B617DAB04A56C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti174E → D in AAA20967 (PubMed:8206394).Curated1
Sequence conflicti269A → V in AAA20967 (PubMed:8206394).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0546031 – 44MRDRT…EFFHK → MGMTARTKRTRSGSRWWCTR ARHGWGARTRSSSTSPLGHP PQ in isoform 2. 1 PublicationAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07158 mRNA Translation: AAA20967.1
X85784 mRNA Translation: CAA59769.1
AJ000541 mRNA Translation: CAA04174.1
AF026007 mRNA Translation: AAB88810.1
AF318489 Transcribed RNA Translation: AAG40313.1
BT007326 mRNA Translation: AAP35990.1
AK091833 mRNA Translation: BAG52424.1
AK315716 mRNA Translation: BAG38075.1
CR541806 mRNA Translation: CAG46605.1
AC135050 Genomic DNA No translation available.
CH471192 Genomic DNA Translation: EAW52176.1
CH471192 Genomic DNA Translation: EAW52177.1
BC002436 mRNA Translation: AAH02436.1
CCDSiCCDS10700.1 [Q12846-1]
CCDS61916.1 [Q12846-2]
PIRiI38517
S52726
RefSeqiNP_001259025.1, NM_001272096.1 [Q12846-2]
NP_004595.2, NM_004604.4 [Q12846-1]
UniGeneiHs.83734

Genome annotation databases

EnsembliENST00000313843; ENSP00000317714; ENSG00000103496 [Q12846-1]
ENST00000394998; ENSP00000378447; ENSG00000103496 [Q12846-2]
GeneIDi6810
KEGGihsa:6810
UCSCiuc002eak.5 human [Q12846-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSTX4_HUMAN
AccessioniPrimary (citable) accession number: Q12846
Secondary accession number(s): A8MXY0, Q15525, Q6FHE8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1998
Last modified: June 20, 2018
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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