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Protein

Syntaxin-4

Gene

STX4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes (By similarity). May also play a role in docking of synaptic vesicles at presynaptic active zones.By similarity

GO - Molecular functioni

  • myosin binding Source: GO_Central
  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: GO_Central
  • sphingomyelin phosphodiesterase activator activity Source: UniProtKB

GO - Biological processi

  • intracellular protein transport Source: GO_Central
  • long-term synaptic potentiation Source: UniProtKB
  • organelle fusion Source: UniProtKB
  • positive regulation of catalytic activity Source: UniProtKB
  • positive regulation of cell adhesion Source: UniProtKB
  • positive regulation of cell migration Source: UniProtKB
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of chemotaxis Source: UniProtKB
  • positive regulation of eosinophil degranulation Source: UniProtKB
  • positive regulation of establishment of protein localization to plasma membrane Source: UniProtKB
  • positive regulation of immunoglobulin secretion Source: UniProtKB
  • positive regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
  • positive regulation of protein localization to cell surface Source: UniProtKB
  • positive regulation of protein localization to plasma membrane Source: UniProtKB
  • post-Golgi vesicle-mediated transport Source: Reactome
  • regulation of exocytosis Source: UniProtKB
  • regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
  • response to hydroperoxide Source: UniProtKB
  • SNARE complex assembly Source: Ensembl
  • synaptic vesicle fusion to presynaptic active zone membrane Source: GO_Central
  • vesicle docking Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000103496-MONOMER.
ReactomeiR-HSA-114516. Disinhibition of SNARE formation.
R-HSA-1236974. ER-Phagosome pathway.
R-HSA-1445148. Translocation of GLUT4 to the plasma membrane.
R-HSA-421837. Clathrin derived vesicle budding.
R-HSA-449147. Signaling by Interleukins.

Protein family/group databases

TCDBi1.F.1.1.1. the synaptosomal vesicle fusion pore (svf-pore) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-4
Alternative name(s):
Renal carcinoma antigen NY-REN-31
Gene namesi
Name:STX4
Synonyms:STX4A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:11439. STX4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 275CytoplasmicSequence analysisAdd BLAST275
Transmembranei276 – 296Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini297ExtracellularSequence analysis1

GO - Cellular componenti

  • basolateral plasma membrane Source: HGNC
  • cell surface Source: UniProtKB
  • cytosol Source: Reactome
  • dendritic spine Source: UniProtKB
  • endosome Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: UniProtKB
  • integral component of membrane Source: GO_Central
  • intracellular Source: UniProtKB
  • lamellipodium Source: UniProtKB
  • lateral loop Source: Ensembl
  • membrane Source: UniProtKB
  • myelin sheath adaxonal region Source: Ensembl
  • perinuclear region of cytoplasm Source: Ensembl
  • plasma membrane Source: UniProtKB
  • SNARE complex Source: UniProtKB
  • somatodendritic compartment Source: UniProtKB
  • specific granule Source: UniProtKB
  • storage vacuole Source: Ensembl
  • synapse Source: UniProtKB
  • synaptic vesicle Source: GO_Central
  • trans-Golgi network Source: Ensembl
  • vacuole Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi6810.
OpenTargetsiENSG00000103496.
PharmGKBiPA36236.

Polymorphism and mutation databases

DMDMi3041737.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002102021 – 297Syntaxin-4Add BLAST297

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineCombined sources1
Modified residuei15PhosphoserineCombined sources1
Modified residuei31PhosphothreonineCombined sources1
Modified residuei36PhosphoserineCombined sources1
Modified residuei117PhosphoserineCombined sources1
Modified residuei208PhosphoserineBy similarity1
Modified residuei248PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ12846.
MaxQBiQ12846.
PaxDbiQ12846.
PeptideAtlasiQ12846.
PRIDEiQ12846.

2D gel databases

OGPiQ12846.

PTM databases

iPTMnetiQ12846.
PhosphoSitePlusiQ12846.
SwissPalmiQ12846.

Expressioni

Tissue specificityi

Expressed in neutrophils and neutrophil-differentiated HL-60 cells. Expression in neutrophils increases with differentiation.1 Publication

Gene expression databases

BgeeiENSG00000103496.
CleanExiHS_STX4.
ExpressionAtlasiQ12846. baseline and differential.
GenevisibleiQ12846. HS.

Organism-specific databases

HPAiHPA001330.

Interactioni

Subunit structurei

Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that interacts with SYT7 during lysosomal exocytosis. Found in a complex with VAMP8 and SNAP23. Detected in a complex with SNAP23 and STXBP4. Interacts with VAMP2. Interacts with SNAP23 and SNAPIN. Interacts with LLGL1. Interacts (via C-terminus) with CENPF. Interacts with DOC2B. Interacts with STXBP6. Interacts with STXBP3; excludes interaction with DOC2B and SNAP25. Interacts with STXBP4; excludes interaction with VAMP2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
BET1O151555EBI-744942,EBI-749204
CYBRD1Q53TN44EBI-744942,EBI-8637742
GIMAP5Q96F153EBI-744942,EBI-6166686
GOSR2O146535EBI-744942,EBI-4401517
NAPAP549202EBI-744942,EBI-749652
NAPBQ9H1155EBI-744942,EBI-3921185
PSMA3P257883EBI-744942,EBI-348380
SEC22AQ96IW75EBI-744942,EBI-8652744
SEC22BO753963EBI-744942,EBI-1058865
SNAP47Q5SQN13EBI-744942,EBI-10244848
STX12Q86Y825EBI-744942,EBI-2691717
STX16O146623EBI-744942,EBI-2853548
STX1AQ166234EBI-744942,EBI-712466
STX3Q132775EBI-744942,EBI-1394295
STX5Q131905EBI-744942,EBI-714206
STX6O437526EBI-744942,EBI-2695795
STX7O154006EBI-744942,EBI-3221827
STX8Q9UNK05EBI-744942,EBI-727240
VAMP1P237633EBI-744942,EBI-10201335
VAMP2P630275EBI-744942,EBI-520113
VAMP3Q158368EBI-744942,EBI-722343
VAMP4O753796EBI-744942,EBI-744953
VAMP4O75379-23EBI-744942,EBI-10187996
VAMP5O951837EBI-744942,EBI-10191195
VTI1BQ9UEU05EBI-744942,EBI-723716

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112679. 64 interactors.
IntActiQ12846. 46 interactors.
MINTiMINT-1454763.
STRINGi9606.ENSP00000317714.

Structurei

3D structure databases

ProteinModelPortaliQ12846.
SMRiQ12846.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini200 – 262t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni154 – 297Interaction with CENPFBy similarityAdd BLAST144

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili43 – 163Sequence analysisAdd BLAST121

Sequence similaritiesi

Belongs to the syntaxin family.Curated
Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0810. Eukaryota.
COG5074. LUCA.
GeneTreeiENSGT00760000119200.
HOGENOMiHOG000286023.
HOVERGENiHBG000497.
InParanoidiQ12846.
KOiK13502.
OMAiYEMSNIN.
OrthoDBiEOG091G0EUK.
PhylomeDBiQ12846.
TreeFamiTF313763.

Family and domain databases

InterProiIPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q12846-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRDRTHELRQ GDDSSDEEDK ERVALVVHPG TARLGSPDEE FFHKVRTIRQ
60 70 80 90 100
TIVKLGNKVQ ELEKQQVTIL ATPLPEESMK QELQNLRDEI KQLGREIRLQ
110 120 130 140 150
LKAIEPQKEE ADENYNSVNT RMRKTQHGVL SQQFVELINK CNSMQSEYRE
160 170 180 190 200
KNVERIRRQL KITNAGMVSD EELEQMLDSG QSEVFVSNIL KDTQVTRQAL
210 220 230 240 250
NEISARHSEI QQLERSIREL HDIFTFLATE VEMQGEMINR IEKNILSSAD
260 270 280 290
YVERGQEHVK TALENQKKAR KKKVLIAICV SITVVLLAVI IGVTVVG
Length:297
Mass (Da):34,180
Last modified:July 15, 1998 - v2
Checksum:i5084FD1C49A86BAA
GO
Isoform 2 (identifier: Q12846-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: MRDRTHELRQ...GSPDEEFFHK → MGMTARTKRT...STSPLGHPPQ

Note: No experimental confirmation available.
Show »
Length:295
Mass (Da):33,841
Checksum:i0E0B617DAB04A56C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti174E → D in AAA20967 (PubMed:8206394).Curated1
Sequence conflicti269A → V in AAA20967 (PubMed:8206394).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0546031 – 44MRDRT…EFFHK → MGMTARTKRTRSGSRWWCTR ARHGWGARTRSSSTSPLGHP PQ in isoform 2. 1 PublicationAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07158 mRNA. Translation: AAA20967.1.
X85784 mRNA. Translation: CAA59769.1.
AJ000541 mRNA. Translation: CAA04174.1.
AF026007 mRNA. Translation: AAB88810.1.
AF318489 Transcribed RNA. Translation: AAG40313.1.
BT007326 mRNA. Translation: AAP35990.1.
AK091833 mRNA. Translation: BAG52424.1.
AK315716 mRNA. Translation: BAG38075.1.
CR541806 mRNA. Translation: CAG46605.1.
AC135050 Genomic DNA. No translation available.
CH471192 Genomic DNA. Translation: EAW52176.1.
CH471192 Genomic DNA. Translation: EAW52177.1.
BC002436 mRNA. Translation: AAH02436.1.
CCDSiCCDS10700.1. [Q12846-1]
CCDS61916.1. [Q12846-2]
PIRiI38517.
S52726.
RefSeqiNP_001259025.1. NM_001272096.1. [Q12846-2]
NP_004595.2. NM_004604.4. [Q12846-1]
UniGeneiHs.83734.

Genome annotation databases

EnsembliENST00000313843; ENSP00000317714; ENSG00000103496. [Q12846-1]
ENST00000394998; ENSP00000378447; ENSG00000103496. [Q12846-2]
GeneIDi6810.
KEGGihsa:6810.
UCSCiuc002eak.5. human. [Q12846-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07158 mRNA. Translation: AAA20967.1.
X85784 mRNA. Translation: CAA59769.1.
AJ000541 mRNA. Translation: CAA04174.1.
AF026007 mRNA. Translation: AAB88810.1.
AF318489 Transcribed RNA. Translation: AAG40313.1.
BT007326 mRNA. Translation: AAP35990.1.
AK091833 mRNA. Translation: BAG52424.1.
AK315716 mRNA. Translation: BAG38075.1.
CR541806 mRNA. Translation: CAG46605.1.
AC135050 Genomic DNA. No translation available.
CH471192 Genomic DNA. Translation: EAW52176.1.
CH471192 Genomic DNA. Translation: EAW52177.1.
BC002436 mRNA. Translation: AAH02436.1.
CCDSiCCDS10700.1. [Q12846-1]
CCDS61916.1. [Q12846-2]
PIRiI38517.
S52726.
RefSeqiNP_001259025.1. NM_001272096.1. [Q12846-2]
NP_004595.2. NM_004604.4. [Q12846-1]
UniGeneiHs.83734.

3D structure databases

ProteinModelPortaliQ12846.
SMRiQ12846.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112679. 64 interactors.
IntActiQ12846. 46 interactors.
MINTiMINT-1454763.
STRINGi9606.ENSP00000317714.

Protein family/group databases

TCDBi1.F.1.1.1. the synaptosomal vesicle fusion pore (svf-pore) family.

PTM databases

iPTMnetiQ12846.
PhosphoSitePlusiQ12846.
SwissPalmiQ12846.

Polymorphism and mutation databases

DMDMi3041737.

2D gel databases

OGPiQ12846.

Proteomic databases

EPDiQ12846.
MaxQBiQ12846.
PaxDbiQ12846.
PeptideAtlasiQ12846.
PRIDEiQ12846.

Protocols and materials databases

DNASUi6810.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000313843; ENSP00000317714; ENSG00000103496. [Q12846-1]
ENST00000394998; ENSP00000378447; ENSG00000103496. [Q12846-2]
GeneIDi6810.
KEGGihsa:6810.
UCSCiuc002eak.5. human. [Q12846-1]

Organism-specific databases

CTDi6810.
DisGeNETi6810.
GeneCardsiSTX4.
HGNCiHGNC:11439. STX4.
HPAiHPA001330.
MIMi186591. gene.
neXtProtiNX_Q12846.
OpenTargetsiENSG00000103496.
PharmGKBiPA36236.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0810. Eukaryota.
COG5074. LUCA.
GeneTreeiENSGT00760000119200.
HOGENOMiHOG000286023.
HOVERGENiHBG000497.
InParanoidiQ12846.
KOiK13502.
OMAiYEMSNIN.
OrthoDBiEOG091G0EUK.
PhylomeDBiQ12846.
TreeFamiTF313763.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000103496-MONOMER.
ReactomeiR-HSA-114516. Disinhibition of SNARE formation.
R-HSA-1236974. ER-Phagosome pathway.
R-HSA-1445148. Translocation of GLUT4 to the plasma membrane.
R-HSA-421837. Clathrin derived vesicle budding.
R-HSA-449147. Signaling by Interleukins.

Miscellaneous databases

ChiTaRSiSTX4. human.
GeneWikiiSTX4.
GenomeRNAii6810.
PROiQ12846.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000103496.
CleanExiHS_STX4.
ExpressionAtlasiQ12846. baseline and differential.
GenevisibleiQ12846. HS.

Family and domain databases

InterProiIPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTX4_HUMAN
AccessioniPrimary (citable) accession number: Q12846
Secondary accession number(s): A8MXY0, Q15525, Q6FHE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1998
Last modified: November 30, 2016
This is version 162 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.