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Protein

Kinesin heavy chain isoform 5A

Gene

KIF5A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. The ZFYVE27-KIF5A complex contributes to the vesicular transport of VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 proteins in neurons.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi86 – 93ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent microtubule motor activity, plus-end-directed Source: GO_Central
  • motor activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000155980-MONOMER.
ReactomeiR-HSA-2132295. MHC class II antigen presentation.
R-HSA-264876. Insulin processing.
R-HSA-5625970. RHO GTPases activate KTN1.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-HSA-983189. Kinesins.
SignaLinkiQ12840.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin heavy chain isoform 5A
Alternative name(s):
Kinesin heavy chain neuron-specific 1
Neuronal kinesin heavy chain
Short name:
NKHC
Gene namesi
Name:KIF5A
Synonyms:NKHC1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:6323. KIF5A.

Subcellular locationi

GO - Cellular componenti

  • ciliary rootlet Source: Ensembl
  • cytosol Source: Reactome
  • kinesin complex Source: ProtInc
  • membrane Source: UniProtKB
  • microtubule Source: UniProtKB-KW
  • neuronal cell body Source: Ensembl
  • neuron projection Source: Ensembl
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Involvement in diseasei

Spastic paraplegia 10, autosomal dominant (SPG10)7 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
See also OMIM:604187
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05874163Y → C in SPG10; complicated form. 1 Publication1
Natural variantiVAR_058742198M → T in SPG10; complicated form. 1 Publication1
Natural variantiVAR_066616203S → C in SPG10. 1 Publication1
Natural variantiVAR_058743204R → Q in SPG10; complicated form. 1 PublicationCorresponds to variant rs387907287dbSNPEnsembl.1
Natural variantiVAR_058744251E → K in SPG10; complicated form. 1 PublicationCorresponds to variant rs387907285dbSNPEnsembl.1
Natural variantiVAR_046744253K → N in SPG10; decreases microtubule affinity; reduces gliding velocity; reduces microtubule-dependent ATP turnover. 2 Publications1
Natural variantiVAR_032842256N → S in SPG10; slightly decreases microtubule affinity; reduces gliding velocity; reduces microtubule-dependent ATP turnover. 2 PublicationsCorresponds to variant rs121434441dbSNPEnsembl.1
Natural variantiVAR_058745256Missing in SPG10. 1 Publication1
Natural variantiVAR_058746257K → N in SPG10; complicated form. 1 Publication1
Natural variantiVAR_033108276Y → C in SPG10. 1 PublicationCorresponds to variant rs121434443dbSNPEnsembl.1
Natural variantiVAR_032843280R → C in SPG10. 2 PublicationsCorresponds to variant rs121434442dbSNPEnsembl.1
Natural variantiVAR_058747280R → H in SPG10; complicated form. 1 PublicationCorresponds to variant rs387907288dbSNPEnsembl.1
Natural variantiVAR_058748280R → L in SPG10; pure form. 1 Publication1
Natural variantiVAR_032844361A → V in SPG10; does not affect microtubule affinity; does not affect gliding velocity; does not affect microtubule-dependent ATP turnover. 2 PublicationsCorresponds to variant rs121434444dbSNPEnsembl.1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi280R → S: Strongly reduces microtubule affinity; slightly reduces gliding velocity. 1 Publication1

Keywords - Diseasei

Disease mutation, Hereditary spastic paraplegia, Neurodegeneration

Organism-specific databases

DisGeNETi3798.
MalaCardsiKIF5A.
MIMi604187. phenotype.
OpenTargetsiENSG00000155980.
Orphaneti324611. Autosomal dominant Charcot-Marie-Tooth disease type 2 due to KIF5A mutation.
100991. Autosomal dominant spastic paraplegia type 10.
PharmGKBiPA30107.

Chemistry databases

ChEMBLiCHEMBL5295.

Polymorphism and mutation databases

BioMutaiKIF5A.
DMDMi143811412.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001253532 – 1032Kinesin heavy chain isoform 5AAdd BLAST1031

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei397PhosphothreonineBy similarity1
Modified residuei931PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ12840.
MaxQBiQ12840.
PaxDbiQ12840.
PeptideAtlasiQ12840.
PRIDEiQ12840.

PTM databases

iPTMnetiQ12840.
PhosphoSitePlusiQ12840.
SwissPalmiQ12840.

Expressioni

Tissue specificityi

Distributed throughout the CNS but is highly enriched in subsets of neurons.

Gene expression databases

BgeeiENSG00000155980.
CleanExiHS_KIF5A.
ExpressionAtlasiQ12840. baseline and differential.
GenevisibleiQ12840. HS.

Organism-specific databases

HPAiCAB015157.
HPA004469.

Interactioni

Subunit structurei

Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1. Interacts with FMR1 (via C-terminus); this interaction is increased in a mGluR-dependent manner (By similarity). Interacts with BORCS5 (PubMed:25898167). Interacts with ZFYVE27. Interacts with VAPA, VAPB, SURF4, RAB11A (GDP-bound form), RAB11B (GDP-bound form) and RTN3 in a ZFYVE27-dependent manner (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ANKRD27Q96NW44EBI-713468,EBI-6125599
Trak2Q8R2H72EBI-713468,EBI-1396483From a different organism.

Protein-protein interaction databases

BioGridi109999. 28 interactors.
DIPiDIP-37584N.
IntActiQ12840. 21 interactors.
MINTiMINT-1370533.
STRINGi9606.ENSP00000408979.

Chemistry databases

BindingDBiQ12840.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4UXTelectron microscopy7.40C1-340[»]
4UXYelectron microscopy6.50C1-340[»]
4UY0electron microscopy7.70C1-340[»]
ProteinModelPortaliQ12840.
SMRiQ12840.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 327Kinesin motorPROSITE-ProRule annotationAdd BLAST319

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni174 – 315Microtubule-bindingAdd BLAST142
Regioni271 – 361Necessary for interaction with ZFYVE27By similarityAdd BLAST91
Regioni907 – 1032GlobularAdd BLAST126

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili331 – 906Add BLAST576

Domaini

Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0240. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00860000133684.
HOGENOMiHOG000216718.
HOVERGENiHBG006210.
InParanoidiQ12840.
KOiK10396.
OMAiMENHREA.
OrthoDBiEOG091G02A2.
PhylomeDBiQ12840.
TreeFamiTF105225.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12840-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAETNNECSI KVLCRFRPLN QAEILRGDKF IPIFQGDDSV VIGGKPYVFD
60 70 80 90 100
RVFPPNTTQE QVYHACAMQI VKDVLAGYNG TIFAYGQTSS GKTHTMEGKL
110 120 130 140 150
HDPQLMGIIP RIARDIFNHI YSMDENLEFH IKVSYFEIYL DKIRDLLDVT
160 170 180 190 200
KTNLSVHEDK NRVPFVKGCT ERFVSSPEEI LDVIDEGKSN RHVAVTNMNE
210 220 230 240 250
HSSRSHSIFL INIKQENMET EQKLSGKLYL VDLAGSEKVS KTGAEGAVLD
260 270 280 290 300
EAKNINKSLS ALGNVISALA EGTKSYVPYR DSKMTRILQD SLGGNCRTTM
310 320 330 340 350
FICCSPSSYN DAETKSTLMF GQRAKTIKNT ASVNLELTAE QWKKKYEKEK
360 370 380 390 400
EKTKAQKETI AKLEAELSRW RNGENVPETE RLAGEEAALG AELCEETPVN
410 420 430 440 450
DNSSIVVRIA PEERQKYEEE IRRLYKQLDD KDDEINQQSQ LIEKLKQQML
460 470 480 490 500
DQEELLVSTR GDNEKVQREL SHLQSENDAA KDEVKEVLQA LEELAVNYDQ
510 520 530 540 550
KSQEVEEKSQ QNQLLVDELS QKVATMLSLE SELQRLQEVS GHQRKRIAEV
560 570 580 590 600
LNGLMKDLSE FSVIVGNGEI KLPVEISGAI EEEFTVARLY ISKIKSEVKS
610 620 630 640 650
VVKRCRQLEN LQVECHRKME VTGRELSSCQ LLISQHEAKI RSLTEYMQSV
660 670 680 690 700
ELKKRHLEES YDSLSDELAK LQAQETVHEV ALKDKEPDTQ DADEVKKALE
710 720 730 740 750
LQMESHREAH HRQLARLRDE INEKQKTIDE LKDLNQKLQL ELEKLQADYE
760 770 780 790 800
KLKSEEHEKS TKLQELTFLY ERHEQSKQDL KGLEETVARE LQTLHNLRKL
810 820 830 840 850
FVQDVTTRVK KSAEMEPEDS GGIHSQKQKI SFLENNLEQL TKVHKQLVRD
860 870 880 890 900
NADLRCELPK LEKRLRATAE RVKALEGALK EAKEGAMKDK RRYQQEVDRI
910 920 930 940 950
KEAVRYKSSG KRGHSAQIAK PVRPGHYPAS SPTNPYGTRS PECISYTNSL
960 970 980 990 1000
FQNYQNLYLQ ATPSSTSDMY FANSCTSSGA TSSGGPLASY QKANMDNGNA
1010 1020 1030
TDINDNRSDL PCGYEAEDQA KLFPLHQETA AS
Length:1,032
Mass (Da):117,378
Last modified:April 3, 2007 - v2
Checksum:iC4C6C12342040796
GO

Sequence cautioni

The sequence BAE06127 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti490A → V in BAE06127 (Ref. 2) Curated1
Sequence conflicti913G → A in AAA20231 (PubMed:7514426).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05874163Y → C in SPG10; complicated form. 1 Publication1
Natural variantiVAR_058742198M → T in SPG10; complicated form. 1 Publication1
Natural variantiVAR_066616203S → C in SPG10. 1 Publication1
Natural variantiVAR_058743204R → Q in SPG10; complicated form. 1 PublicationCorresponds to variant rs387907287dbSNPEnsembl.1
Natural variantiVAR_058744251E → K in SPG10; complicated form. 1 PublicationCorresponds to variant rs387907285dbSNPEnsembl.1
Natural variantiVAR_046744253K → N in SPG10; decreases microtubule affinity; reduces gliding velocity; reduces microtubule-dependent ATP turnover. 2 Publications1
Natural variantiVAR_032842256N → S in SPG10; slightly decreases microtubule affinity; reduces gliding velocity; reduces microtubule-dependent ATP turnover. 2 PublicationsCorresponds to variant rs121434441dbSNPEnsembl.1
Natural variantiVAR_058745256Missing in SPG10. 1 Publication1
Natural variantiVAR_058746257K → N in SPG10; complicated form. 1 Publication1
Natural variantiVAR_033108276Y → C in SPG10. 1 PublicationCorresponds to variant rs121434443dbSNPEnsembl.1
Natural variantiVAR_032843280R → C in SPG10. 2 PublicationsCorresponds to variant rs121434442dbSNPEnsembl.1
Natural variantiVAR_058747280R → H in SPG10; complicated form. 1 PublicationCorresponds to variant rs387907288dbSNPEnsembl.1
Natural variantiVAR_058748280R → L in SPG10; pure form. 1 Publication1
Natural variantiVAR_032844361A → V in SPG10; does not affect microtubule affinity; does not affect gliding velocity; does not affect microtubule-dependent ATP turnover. 2 PublicationsCorresponds to variant rs121434444dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06698 mRNA. Translation: AAA20231.1.
AB210045 mRNA. Translation: BAE06127.1. Different initiation.
CH471054 Genomic DNA. Translation: EAW97030.1.
BC146670 mRNA. Translation: AAI46671.1.
BC150208 mRNA. Translation: AAI50209.1.
CCDSiCCDS8945.1.
PIRiI38510.
RefSeqiNP_004975.2. NM_004984.2.
UniGeneiHs.151219.

Genome annotation databases

EnsembliENST00000455537; ENSP00000408979; ENSG00000155980.
GeneIDi3798.
KEGGihsa:3798.
UCSCiuc001sor.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06698 mRNA. Translation: AAA20231.1.
AB210045 mRNA. Translation: BAE06127.1. Different initiation.
CH471054 Genomic DNA. Translation: EAW97030.1.
BC146670 mRNA. Translation: AAI46671.1.
BC150208 mRNA. Translation: AAI50209.1.
CCDSiCCDS8945.1.
PIRiI38510.
RefSeqiNP_004975.2. NM_004984.2.
UniGeneiHs.151219.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4UXTelectron microscopy7.40C1-340[»]
4UXYelectron microscopy6.50C1-340[»]
4UY0electron microscopy7.70C1-340[»]
ProteinModelPortaliQ12840.
SMRiQ12840.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109999. 28 interactors.
DIPiDIP-37584N.
IntActiQ12840. 21 interactors.
MINTiMINT-1370533.
STRINGi9606.ENSP00000408979.

Chemistry databases

BindingDBiQ12840.
ChEMBLiCHEMBL5295.

PTM databases

iPTMnetiQ12840.
PhosphoSitePlusiQ12840.
SwissPalmiQ12840.

Polymorphism and mutation databases

BioMutaiKIF5A.
DMDMi143811412.

Proteomic databases

EPDiQ12840.
MaxQBiQ12840.
PaxDbiQ12840.
PeptideAtlasiQ12840.
PRIDEiQ12840.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000455537; ENSP00000408979; ENSG00000155980.
GeneIDi3798.
KEGGihsa:3798.
UCSCiuc001sor.2. human.

Organism-specific databases

CTDi3798.
DisGeNETi3798.
GeneCardsiKIF5A.
H-InvDBHIX0036852.
HGNCiHGNC:6323. KIF5A.
HPAiCAB015157.
HPA004469.
MalaCardsiKIF5A.
MIMi602821. gene.
604187. phenotype.
neXtProtiNX_Q12840.
OpenTargetsiENSG00000155980.
Orphaneti324611. Autosomal dominant Charcot-Marie-Tooth disease type 2 due to KIF5A mutation.
100991. Autosomal dominant spastic paraplegia type 10.
PharmGKBiPA30107.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0240. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00860000133684.
HOGENOMiHOG000216718.
HOVERGENiHBG006210.
InParanoidiQ12840.
KOiK10396.
OMAiMENHREA.
OrthoDBiEOG091G02A2.
PhylomeDBiQ12840.
TreeFamiTF105225.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000155980-MONOMER.
ReactomeiR-HSA-2132295. MHC class II antigen presentation.
R-HSA-264876. Insulin processing.
R-HSA-5625970. RHO GTPases activate KTN1.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-HSA-983189. Kinesins.
SignaLinkiQ12840.

Miscellaneous databases

ChiTaRSiKIF5A. human.
GeneWikiiKIF5A.
GenomeRNAii3798.
PROiQ12840.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000155980.
CleanExiHS_KIF5A.
ExpressionAtlasiQ12840. baseline and differential.
GenevisibleiQ12840. HS.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKIF5A_HUMAN
AccessioniPrimary (citable) accession number: Q12840
Secondary accession number(s): A6H8M5, Q4LE26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 3, 2007
Last modified: November 30, 2016
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.