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Protein

Zona pellucida sperm-binding protein 4

Gene

ZP4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP4 may act as a sperm receptor.

GO - Molecular functioni

  • acrosin binding Source: UniProtKB
  • identical protein binding Source: IntAct

GO - Biological processi

  • acrosomal vesicle exocytosis Source: UniProtKB
  • binding of sperm to zona pellucida Source: Reactome
  • negative regulation of binding of sperm to zona pellucida Source: UniProtKB
  • positive regulation of acrosome reaction Source: UniProtKB
  • positive regulation of humoral immune response Source: UniProtKB
  • positive regulation of T cell proliferation Source: UniProtKB

Keywordsi

Molecular functionReceptor
Biological processFertilization

Enzyme and pathway databases

ReactomeiR-HSA-1300644 Interaction With The Zona Pellucida

Names & Taxonomyi

Protein namesi
Recommended name:
Zona pellucida sperm-binding protein 4
Alternative name(s):
Zona pellucida glycoprotein 4
Short name:
Zp-4
Zona pellucida protein B
Cleaved into the following chain:
Gene namesi
Name:ZP4
Synonyms:ZPB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000116996.9
HGNCiHGNC:15770 ZP4
MIMi613514 gene
neXtProtiNX_Q12836

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 505ExtracellularSequence analysisAdd BLAST487
Transmembranei506 – 526HelicalSequence analysisAdd BLAST21
Topological domaini527 – 540CytoplasmicSequence analysisAdd BLAST14

Keywords - Cellular componenti

Cell membrane, Extracellular matrix, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi57829
OpenTargetsiENSG00000116996
PharmGKBiPA38036

Polymorphism and mutation databases

BioMutaiZP4
DMDMi46397080

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000004172719 – 462Zona pellucida sperm-binding protein 4Add BLAST444
ChainiPRO_000030457819 – ?Processed zona pellucida sperm-binding protein 4
PropeptideiPRO_0000041728463 – 540Removed in mature formBy similarityAdd BLAST78

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi69N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi202N-linked (GlcNAc...) asparagineBy similarity1
Glycosylationi219N-linked (GlcNAc...) asparagineBy similarity1
Glycosylationi267N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi302O-linked (GalNAc...) threonineBy similarity1
Disulfide bondi367 ↔ 442PROSITE-ProRule annotation
Glycosylationi470N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi474N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ12836
PeptideAtlasiQ12836
PRIDEiQ12836
ProteomicsDBi58977

PTM databases

iPTMnetiQ12836
PhosphoSitePlusiQ12836

Expressioni

Tissue specificityi

Oocytes.

Gene expression databases

BgeeiENSG00000116996
CleanExiHS_ZP4
GenevisibleiQ12836 HS

Organism-specific databases

HPAiHPA008547

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself6EBI-11783805,EBI-11783805

GO - Molecular functioni

  • acrosin binding Source: UniProtKB
  • identical protein binding Source: IntAct

Protein-protein interaction databases

MINTiQ12836
STRINGi9606.ENSP00000355529

Structurei

3D structure databases

ProteinModelPortaliQ12836
SMRiQ12836
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini141 – 183P-typePROSITE-ProRule annotationAdd BLAST43
Domaini188 – 466ZPPROSITE-ProRule annotationAdd BLAST279

Domaini

The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

Sequence similaritiesi

Belongs to the ZP domain family. ZPB subfamily.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IE4J Eukaryota
ENOG4111H75 LUCA
GeneTreeiENSGT00530000063096
HOGENOMiHOG000059607
HOVERGENiHBG050410
InParanoidiQ12836
OMAiTRDSIFR
OrthoDBiEOG091G0UNU
PhylomeDBiQ12836
TreeFamiTF332794

Family and domain databases

CDDicd00111 Trefoil, 1 hit
InterProiView protein in InterPro
IPR017957 P_trefoil_CS
IPR000519 P_trefoil_dom
IPR001507 ZP_dom
IPR017977 ZP_dom_CS
PfamiView protein in Pfam
PF00088 Trefoil, 1 hit
PF00100 Zona_pellucida, 1 hit
PRINTSiPR00023 ZPELLUCIDA
SMARTiView protein in SMART
SM00018 PD, 1 hit
SM00241 ZP, 1 hit
PROSITEiView protein in PROSITE
PS00025 P_TREFOIL_1, 1 hit
PS51448 P_TREFOIL_2, 1 hit
PS00682 ZP_1, 1 hit
PS51034 ZP_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12836-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLLRCVLLC VSLSLAVSGQ HKPEAPDYSS VLHCGPWSFQ FAVNLNQEAT
60 70 80 90 100
SPPVLIAWDN QGLLHELQND SDCGTWIRKG PGSSVVLEAT YSSCYVTEWD
110 120 130 140 150
SHYIMPVGVE GAGAAEHKVV TERKLLKCPM DLLARDAPDT DWCDSIPARD
160 170 180 190 200
RLPCAPSPIS RGDCEGLGCC YSSEEVNSCY YGNTVTLHCT REGHFSIAVS
210 220 230 240 250
RNVTSPPLLL DSVRLALRND SACNPVMATQ AFVLFQFPFT SCGTTRQITG
260 270 280 290 300
DRAVYENELV ATRDVKNGSR GSVTRDSIFR LHVSCSYSVS SNSLPINVQV
310 320 330 340 350
FTLPPPFPET QPGPLTLELQ IAKDKNYGSY YGVGDYPVVK LLRDPIYVEV
360 370 380 390 400
SILHRTDPYL GLLLQQCWAT PSTDPLSQPQ WPILVKGCPY IGDNYQTQLI
410 420 430 440 450
PVQKALDLPF PSHHQRFSIF TFSFVNPTVE KQALRGPVHL HCSVSVCQPA
460 470 480 490 500
ETPSCVVTCP DLSRRRNFDN SSQNTTASVS SKGPMILLQA TKDPPEKLRV
510 520 530 540
PVDSKVLWVA GLSGTLILGA LLVSYLAVKK QKSCPDQMCQ
Length:540
Mass (Da):59,400
Last modified:November 1, 1996 - v1
Checksum:i9DAC310D808E1AC3
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052997114A → V. Corresponds to variant dbSNP:rs34370253Ensembl.1
Natural variantiVAR_052998295P → S. Corresponds to variant dbSNP:rs34811980Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05781 Genomic DNA Translation: AAA74391.1
AK314151 mRNA Translation: BAG36838.1
AL359924 Genomic DNA No translation available.
CH471098 Genomic DNA Translation: EAW70072.1
BC069521 mRNA Translation: AAH69521.1
CCDSiCCDS1615.1
PIRiS70403
RefSeqiNP_067009.1, NM_021186.4
UniGeneiHs.136241

Genome annotation databases

EnsembliENST00000366570; ENSP00000355529; ENSG00000116996
ENST00000611898; ENSP00000482304; ENSG00000116996
GeneIDi57829
KEGGihsa:57829
UCSCiuc001hym.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiZP4_HUMAN
AccessioniPrimary (citable) accession number: Q12836
Secondary accession number(s): B2RAE1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: November 1, 1996
Last modified: June 20, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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