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Reviewed, UniProtKB/Swiss-Prot Q12834 (CDC20_HUMAN)

Last modified November 25, 2008. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Cell division cycle protein 20 homolog
Alternative name(s):
    p55CDC
Gene names
Name: CDC20
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with SPATC1 By similarity. Interacts with MAD2L1. The phosphorylated form interacts with APC/C.

Developmental stage

Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.

Post-translational modification

Phosphorylated during mitosis, probably by maturation promoting factor (MPF).

Sequence similarities

Belongs to the WD repeat CDC20/Fizzy family.

Contains 7 WD repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 499499Cell division cycle protein 20 homolog
PRO_0000050900

Regions

Repeat182 – 22140WD 1
Repeat224 – 26340WD 2
Repeat266 – 30338WD 3
Repeat307 – 34640WD 4
Repeat353 – 39543WD 5
Repeat397 – 43842WD 6
Repeat441 – 48040WD 7

Amino acid modifications

Modified residue411Phosphoserine
Modified residue701Phosphothreonine
Modified residue1061Phosphothreonine

Natural variations

Natural variant4021V → M
VAR_030368
Natural variant4791R → Q
VAR_030369

Experimental info

Sequence conflict1011P → S in AAA19017. Ref.1
Sequence conflict1171A → V in AAH00624. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q12834-1 [UniParc].

Last modified October 3, 2003. Version 2.
Checksum: FD5C967AF84089E8

FASTA49954,723
        10         20         30         40         50         60 
MAQFAFESDL HSLLQLDAPI PNAPPARWQR KAKEAAGPAP SPMRAANRSH SAGRTPGRTP 

        70         80         90        100        110        120 
GKSSSKVQTT PSKPGGDRYI PHRSAAQMEV ASFLLSKENQ PENSQTPTKK EHQKAWALNL 

       130        140        150        160        170        180 
NGFDVEEAKI LRLSGKPQNA PEGYQNRLKV LYSQKATPGS SRKTCRYIPS LPDRILDAPE 

       190        200        210        220        230        240 
IRNDYYLNLV DWSSGNVLAV ALDNSVYLWS ASSGDILQLL QMEQPGEYIS SVAWIKEGNY 

       250        260        270        280        290        300 
LAVGTSSAEV QLWDVQQQKR LRNMTSHSAR VGSLSWNSYI LSSGSRSGHI HHHDVRVAEH 

       310        320        330        340        350        360 
HVATLSGHSQ EVCGLRWAPD GRHLASGGND NLVNVWPSAP GEGGWVPLQT FTQHQGAVKA 

       370        380        390        400        410        420 
VAWCPWQSNV LATGGGTSDR HIRIWNVCSG ACLSAVDAHS QVCSILWSPH YKELISGHGF 

       430        440        450        460        470        480 
AQNQLVIWKY PTMAKVAELK GHTSRVLSLT MSPDGATVAS AAADETLRLW RCFELDPARR 

       490 
REREKASAAK SSLIHQGIR 

« Hide

References

« Hide 'large scale' references
[1]"A novel mammalian protein, p55CDC, present in dividing cells is associated with protein kinase activity and has homology to the Saccharomyces cerevisiae cell division cycle proteins Cdc20 and Cdc4."
Weinstein J., Jacobsen F.W., Hsu-Chen J., Wu T., Baum L.G.
Mol. Cell. Biol. 14:3350-3363(1994) [PubMed: 7513050] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Activation of the human anaphase-promoting complex by proteins of the CDC20/Fizzy family."
Kramer E.R., Gieffers C., Hoelzl G., Hengstschlaeger M., Peters J.-M.
Curr. Biol. 8:1207-1210(1998) [PubMed: 9811605] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH MAD2L1 AND APC/C.
Tissue: Liver and Spleen.
[3]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu)."
Livingston R.J., Rieder M.J., Shaffer T., Bertucci C., Baier C.N., Rajkumar N., Willa H.T., Daniels M., Downing T.K., Stanaway I.B., Nguyen C.P., Gildersleeve H., Cassidy C.M., Johnson E.J., Swanson J.E., McFarland I., Yool B., Park C., Nickerson D.A.
Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS MET-402 AND GLN-479.
[5]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed: 16710414] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon, Colon adenocarcinoma, Lymph, Muscle, Ovary and Skin.
[7]"Direct binding of CDC20 protein family members activates the anaphase-promoting complex in mitosis and G1."
Fang G., Yu H., Kirschner M.W.
Mol. Cell 2:163-171(1998) [PubMed: 9734353] [Abstract]
Cited for: FUNCTION, INTERACTION WITH APC/C.
[8]"The checkpoint protein MAD2 and the mitotic regulator CDC20 form a ternary complex with the anaphase-promoting complex to control anaphase initiation."
Fang G., Yu H., Kirschner M.W.
Genes Dev. 12:1871-1883(1998) [PubMed: 9637688] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MAD2L1 AND APC/C.
[9]"Regulation of APC activity by phosphorylation and regulatory factors."
Kotani S., Tanaka H., Yasuda H., Todokoro K.
J. Cell Biol. 146:791-800(1999) [PubMed: 10459014] [Abstract]
Cited for: PHOSPHORYLATION.
[10]"Mitotic regulation of the human anaphase-promoting complex by phosphorylation."
Kraft C., Herzog F., Gieffers C., Mechtler K., Hagting A., Pines J., Peters J.-M.
EMBO J. 22:6598-6609(2003) [PubMed: 14657031] [Abstract]
Cited for: PHOSPHORYLATION AT THR-70 AND THR-106.
[11]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

U05340 mRNA. Translation: AAA19017.1.
AF099644 mRNA. Translation: AAD16405.1.
BT007388 mRNA. Translation: AAP36052.1.
DQ473545 Genomic DNA. Translation: ABE96834.1.
AL139289 Genomic DNA. Translation: CAB92757.1.
BC000624 mRNA. Translation: AAH00624.1.
BC001088 mRNA. Translation: AAH01088.1.
BC006272 mRNA. Translation: AAH06272.1.
BC009425 mRNA. Translation: AAH09425.1.
BC009426 mRNA. Translation: AAH09426.1.
BC010044 mRNA. Translation: AAH10044.1.
BC012803 mRNA. Translation: AAH12803.1.
BC012827 mRNA. Translation: AAH12827.1.
BC013303 mRNA. Translation: AAH13303.1.
BC015998 mRNA. Translation: AAH15998.1.
BC024257 mRNA. Translation: AAH24257.1.
BC031294 mRNA. Translation: AAH31294.1.
BC110321 mRNA. Translation: AAI10322.1.
PIRA56021.
RefSeqNP_001246.2.
UniGeneHs.524947

3D structure databases

HSSPHSSP built from PDB template 1ERJ based on UniProtKB P16649.
ModBaseSearch...

Protein-protein interaction databases

IntActQ12834.

PTM databases

PhosphoSiteQ12834.

Polymorphism databases

NIEHS-SNPsSearch...

Genome annotation databases

EnsemblENSG00000117399. Homo sapiens. [Contig view]
GeneID991.
KEGGhsa:991.

Organism-specific databases

HGNCHGNC:1723. CDC20.
HPACAB004525.
MIM603618. gene.
PharmGKBPA26257.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMQ12834.
HOVERGENQ12834.

Enzyme and pathway databases

ReactomeREACT_152. Cell Cycle, Mitotic.
REACT_1538. Cell Cycle Checkpoints.
REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_8017. APC-Cdc20 mediated degradation of Nek2A.

Gene expression databases

CleanExHS_CDC20.
GermOnlineENSG00000117399. Homo sapiens.

Family and domain databases

InterProIPR015943. WD40/YVTN_repeat-like.
IPR001680. WD40_repeat.
[Graphical view]
Gene3DG3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
PfamPF00400. WD40. 6 hits.
[Graphical view]
PRINTSPR00320. GPROTEINBRPT.
ProDomPD004563. Fizzy. 1 hit.
PD000018. WD40. 2 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00320. WD40. 7 hits.
[Graphical view]
PROSITEPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

LinkHubQ12834.
NextBio4160.
SOURCESearch...

Entry information

Entry nameCDC20_HUMAN
AccessionPrimary (citable) accession number: Q12834
Secondary accession number(s): Q5JUY4, Q9BW56, Q9UQI9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: October 3, 2003
Last modified: November 25, 2008
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents