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Reviewed, UniProtKB/Swiss-Prot Q12802 (AKP13_HUMAN)

Last modified July 22, 2008. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    A-kinase anchor protein 13
      Short name=AKAP 13
Alternative name(s):
    Protein kinase A-anchoring protein 13
    Breast cancer nuclear receptor-binding auxiliary protein
    Human thyroid-anchoring protein 31
    Guanine nucleotide exchange factor Lbc
    AKAP-Lbc
    P47
    Lymphoid blast crisis oncogene
      Short name=LBC oncogene
    Non-oncogenic Rho GTPase-specific GTP exchange factor
Gene names
Name: AKAP13
Synonyms: BRX, HT31, LBC
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length2813 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Anchors cAMP-dependent protein kinase (PKA) and acts as an adapter protein to selectively couple G alpha-13 and Rho. Augments gene activation by the estrogen receptor in an element-specific and ligand-dependent manner. Activates estrogen receptor beta by a p38 MAPK-dependent pathway. Isoform 6 stimulates exchange activity on Rho proteins in vitro, but not on CDC42, Ras or Rac and may bind calcium ions.

Subunit structure

Binds cAMP-dependent protein kinase (PKA) and to the RII-alpha regulatory subunit of PKA. Interacts with ESR1, ESR2, THRA, PPARA, RHOA and NME2.

Subcellular location

Isoform 3: Cytoplasm. Nucleus.

Isoform 2: Cytoplasm.

Isoform 6: Cytoplasm.

Isoform 7: Membrane.

Tissue specificity

Isoform 3 and isoform 6 are found in hematopoietic cells, skeletal muscle, lung, heart, estrogen-responsive reproductive tissues, including breast ductal epithelium. Also found in testis and breast cancer cell lines. Isoform 6 is not found in brain, placenta, liver, pancreas or kidney. Isoform 7 is expressed in myeloid and lymphoid lineages, a variety of epithelial tissues and skeletal muscle. Isoform 2 is predominantly found in the heart and at lower levels in the lung, placenta, kidney, pancreas, skeletal muscle and liver.

Domain

Both the DH and PH domains are required for transforming activity.

Sequence similarities

Contains 1 DH (DBL-homology) domain.

Contains 1 PH domain.

Contains 1 phorbol-ester/DAG-type zinc finger.

Sequence caution

The sequence AAD21311.1 differs from that shown. Reason: Frameshift at positions 2614 and 2647.

The sequence AAL40923.1 differs from that shown. Reason: Frameshift at positions 2614 and 2647.

Ontologies

Binary interactions

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q12802-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q12802-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1583-1598: SMRVLGDVVRRPPIHR → MSWCPSGVQYSAGLSADFNY
Isoform 3 (identifier: Q12802-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1384: Missing.
     1385-1387: TQA → MLY
Isoform 4 (identifier: Q12802-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1581-1598: Missing.
     1950-1951: Missing.
Isoform 5 (identifier: Q12802-5)

The sequence of this isoform differs from the canonical sequence as follows:
     160-179: DAGPRETLMHFAVRLGLLRL → GENLYDLQTHFKFVIFLLFF
     180-2813: Missing.
Isoform 6 (identifier: Q12802-6)

Also known as: LBC;

The sequence of this isoform differs from the canonical sequence as follows:
     1-1918: Missing.
     1950-1951: Missing.
     2336-2344: NRDEDEGIP → SGNGWRCFN
     2345-2813: Missing.
Isoform 7 (identifier: Q12802-7)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1918: Missing.
     1950-1951: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 28132813A-kinase anchor protein 13

Regions

Domain1994 – 2191198DH
Domain2231 – 2333103PH
Zinc finger1791 – 183848Phorbol-ester/DAG-type
Region494 – 51623RII-binding
Region1919 – 2813895Interaction with ESR1
Coiled coil1758 – 179033 Potential
Coiled coil2345 – 238137 Potential
Coiled coil2568 – 2683116 Potential
Compositional bias1471 – 14744Poly-Ser
Compositional bias1534 – 15374Poly-Glu
Compositional bias2778 – 279013Poly-Lys

Amino acid modifications

Modified residue9831Phosphoserine
Modified residue19291Phosphoserine
Modified residue19321Phosphoserine
Modified residue27281Phosphoserine

Natural variations

Alternative sequence1 – 19181918Missing in isoform 6 and isoform 7.
Alternative sequence1 – 13841384Missing in isoform 3.
Alternative sequence160 – 17920DAGPR…GLLRL → GENLYDLQTHFKFVIFLLFF in isoform 5.
Alternative sequence180 – 28132634Missing in isoform 5.
Alternative sequence1385 – 13873TQA → MLY in isoform 3.
Alternative sequence1581 – 159818Missing in isoform 4.
Alternative sequence1583 – 159816SMRVL…PPIHR → MSWCPSGVQYSAGLSADFNY in isoform 2.
Alternative sequence1950 – 19512Missing in isoform 4, isoform 6 and isoform 7.
Alternative sequence2336 – 23449NRDEDEGIP → SGNGWRCFN in isoform 6.
Alternative sequence2345 – 2813469Missing in isoform 6.
Natural variant4521M → T: dbSNP rs2061821.
Natural variant4941W → R: dbSNP rs2061822.
Natural variant5741R → C: dbSNP rs2061824.
Natural variant6241G → V: dbSNP rs745191.
Natural variant6891E → K: dbSNP rs7177107.
Natural variant8451V → A: dbSNP rs4075256.
Natural variant8971V → M: dbSNP rs4075254.
Natural variant10621P → A: dbSNP rs4843074.
Natural variant10861D → N: dbSNP rs4843075.
Natural variant12161M → T: dbSNP rs7162168.
Natural variant24571G → S: dbSNP rs2241268.
Natural variant28011A → T: dbSNP rs2614668.

Experimental info

Mutagenesis12511A → P: Loss of PKA anchoring; when associated with P-1260
Mutagenesis12601I → P: Loss of PKA anchoring; when associated with P-1251
Mutagenesis12651A → P: Abolishes RII-binding
Mutagenesis21531Y → F: Loss of interaction with RHOA
Sequence conflict1911G → R in AAL40923. Ref.5
Sequence conflict3541C → R in AAL40923. Ref.5
Sequence conflict6091A → V in AAL40923. Ref.5
Sequence conflict6141A → T in AAL40923. Ref.5
Sequence conflict6181S → P in AAL40923. Ref.5
Sequence conflict6191D → A in AAL11723. Ref.6
Sequence conflict7551M → S in AAA58670. Ref.9
Sequence conflict15471N → H in BAD92651. Ref.10
Sequence conflict20351V → D in AAD21311. Ref.2
Sequence conflict24881D → G in AAD21311. Ref.2
Sequence conflict2667 – 26682QL → HV in AAD21311. Ref.2
Sequence conflict2667 – 26682QL → HV in BAB62913. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 6, 2007. Version 2.
Checksum: 6A8FDAC9A3D3B1F2

FASTA2,813307,550
        10         20         30         40         50         60 
MKLNPQQAPL YGDCVVTVLL AEEDKAEDDV VFYLVFLGST LRHCTSTRKV SSDTLETIAP 

        70         80         90        100        110        120 
GHDCCETVKV QLCASKEGLP VFVVAEEDFH FVQDEAYDAA QFLATSAGNQ QALNFTRFLD 

       130        140        150        160        170        180 
QSGPPSGDVN SLDKKLVLAF RHLKLPTEWN VLGTDQSLHD AGPRETLMHF AVRLGLLRLT 

       190        200        210        220        230        240 
WFLLQKPGGR GALSIHNQEG ATPVSLALER GYHKLHQLLT EENAGEPDSW SSLSYEIPYG 

       250        260        270        280        290        300 
DCSVRHHREL DIYTLTSESD SHHEHPFPGD GCTGPIFKLM NIQQQLMKTN LKQMDSLMPL 

       310        320        330        340        350        360 
MMTAQDPSSA PETDGQFLPC APEPTDPQRL SSSEETESTQ CCPGSPVAQT ESPCDLSSIV 

       370        380        390        400        410        420 
EEENTDRSCR KKNKGVERKG EEVEPAPIVD SGTVSDQDSC LQSLPDCGVK GTEGLSSCGN 

       430        440        450        460        470        480 
RNEETGTKSS GMPTDQESLS SGDAVLQRDL VMEPGTAQYS SGGELGGIST TNVSTPDTAG 

       490        500        510        520        530        540 
EMEHGLMNPD ATVWKNVLQG GESTKERFEN SNIGTAGASD VHVTSKPVDK ISVPNCAPAA 

       550        560        570        580        590        600 
SSLDGNKPAE SSLAFSNEET STEKTAETET SRSREESADA PVDQNSVVIP AAAKDKISDG 

       610        620        630        640        650        660 
LEPYTLLAAG IGEAMSPSDL ALLGLEEDVM PHQNSETNSS HAQSQKGKSS PICSTTGDDK 

       670        680        690        700        710        720 
LCADSACQQN TVTSSGDLVA KLCDNIVSES ESTTARQPSS QDPPDASHCE DPQAHTVTSD 

       730        740        750        760        770        780 
PVRDTQERAD FCPFKVVDNK GQRKDVKLDK PLTNMLEVVS HPHPVVPKME KELVPDQAVI 

       790        800        810        820        830        840 
SDSTFSLANS PGSESVTKDD ALSFVPSQKE KGTATPELHT ATDYRDGPDG NSNEPDTRPL 

       850        860        870        880        890        900 
EDRAVGLSTS STAAELQHGM GNTSLTGLGG EHEGPAPPAI PEALNIKGNT DSSLQSVGKA 

       910        920        930        940        950        960 
TLALDSVLTE EGKLLVVSES SAAQEQDKDK AVTCSSIKEN ALSSGTLQEE QRTPPPGQDT 

       970        980        990       1000       1010       1020 
QQFHEKSISA DCAKDKALQL SNSPGASSAF LKAETEHNKE VAPQVSLLTQ GGAAQSLVPP 

      1030       1040       1050       1060       1070       1080 
GASLATESRQ EALGAEHNSS ALLPCLLPDG SDGSDALNCS QPSPLDVGVK NTQSQGKTSA 

      1090       1100       1110       1120       1130       1140 
CEVSGDVTVD VTGVNALQGM AEPRRENISH NTQDILIPNV LLSQEKNAVL GLPVALQDKA 

      1150       1160       1170       1180       1190       1200 
VTDPQGVGTP EMIPLDWEKG KLEGADHSCT MGDAEEAQID DEAHPVLLQP VAKELPTDME 

      1210       1220       1230       1240       1250       1260 
LSAHDDGAPA GVREVMRAPP SGRERSTPSL PCMVSAQDAP LPKGADLIEE AASRIVDAVI 

      1270       1280       1290       1300       1310       1320 
EQVKAAGALL TEGEACHMSL SSPELGPLTK GLESAFTEKV STFPPGESLP MGSTPEEATG 

      1330       1340       1350       1360       1370       1380 
SLAGCFAGRE EPEKIILPVQ GPEPAAEMPD VKAEDEVDFR ASSISEEVAV GSIAATLKMK 

      1390       1400       1410       1420       1430       1440 
QGPMTQAINR ENWCTIEPCP DAASLLASKQ SPECENFLDV GLGRECTSKQ GVLKRESGSD 

      1450       1460       1470       1480       1490       1500 
SDLFHSPSDD MDSIIFPKPE EEHLACDITG SSSSTDDTAS LDRHSSHGSD VSLSQILKPN 

      1510       1520       1530       1540       1550       1560 
RSRDRQSLDG FYSHGMGAEG RESESEPADP GDVEEEEMDS ITEVPANCSV LRSSMRSLSP 

      1570       1580       1590       1600       1610       1620 
FRRHSWGPGK NAASDAEMNH RSSMRVLGDV VRRPPIHRRS FSLEGLTGGA GVGNKPSSSL 

      1630       1640       1650       1660       1670       1680 
EVSSANAEEL RHPFSGEERV DSLVSLSEED LESDQREHRM FDQQICHRSK QQGFNYCTSA 

      1690       1700       1710       1720       1730       1740 
ISSPLTKSIS LMTISHPGLD NSRPFHSTFH NTSANLTESI TEENYNFLPH SPSKKDSEWK 

      1750       1760       1770       1780       1790       1800 
SGTKVSRTFS YIKNKMSSSK KSKEKEKEKD KIKEKEKDSK DKEKDKKTVN GHTFSSIPVV 

      1810       1820       1830       1840       1850       1860 
GPISCSQCMK PFTNKDAYTC ANCSAFVHKG CRESLASCAK VKMKQPKGSL QAHDTSSLPT 

      1870       1880       1890       1900       1910       1920 
VIMRNKPSQP KERPRSAVLL VDETATTPIF ANRRSQQSVS LSKSVSIQNI TGVGNDENMS 

      1930       1940       1950       1960       1970       1980 
NTWKFLSHST DSLNKISKVN ESTESLTDEG VGTDMNEGQL LGDFEIESKQ LEAESWSRII 

      1990       2000       2010       2020       2030       2040 
DSKFLKQQKK DVVKRQEVIY ELMQTEFHHV RTLKIMSGVY SQGMMADLLF EQQMVEKLFP 

      2050       2060       2070       2080       2090       2100 
CLDELISIHS QFFQRILERK KESLVDKSEK NFLIKRIGDV LVNQFSGENA ERLKKTYGKF 

      2110       2120       2130       2140       2150       2160 
CGQHNQSVNY FKDLYAKDKR FQAFVKKKMS SSVVRRLGIP ECILLVTQRI TKYPVLFQRI 

      2170       2180       2190       2200       2210       2220 
LQCTKDNEVE QEDLAQSLSL VKDVIGAVDS KVASYEKKVR LNEIYTKTDS KSIMRMKSGQ 

      2230       2240       2250       2260       2270       2280 
MFAKEDLKRK KLVRDGSVFL KNAAGRLKEV QAVLLTDILV FLQEKDQKYI FASLDQKSTV 

      2290       2300       2310       2320       2330       2340 
ISLKKLIVRE VAHEEKGLFL ISMGMTDPEM VEVHASSKEE RNSWIQIIQD TINTLNRDED 

      2350       2360       2370       2380       2390       2400 
EGIPSENEEE KKMLDTRARE LKEQLHQKDQ KILLLLEEKE MIFRDMAECS TPLPEDCSPT 

      2410       2420       2430       2440       2450       2460 
HSPRVLFRSN TEEALKGGPL MKSAINEVEI LQGLVSGNLG GTLGPTVSSP IEQDVVGPVS 

      2470       2480       2490       2500       2510       2520 
LPRRAETFGG FDSHQMNASK GGEKEEGDDG QDLRRTESDS GLKKGGNANL VFMLKRNSEQ 

      2530       2540       2550       2560       2570       2580 
VVQSVVHLYE LLSALQGVVL QQDSYIEDQK LVLSERALTR SLSRPSSLIE QEKQRSLEKQ 

      2590       2600       2610       2620       2630       2640 
RQDLANLQKQ QAQYLEEKRR REREWEARER ELREREALLA QREEEVQQGQ QDLEKEREEL 

      2650       2660       2670       2680       2690       2700 
QQKKGTYQYD LERLRAAQKQ LEREQEQLRR EAERLSQRQT ERDLCQVSHP HTKLMRIPSF 

      2710       2720       2730       2740       2750       2760 
FPSPEEPPSP SAPSIAKSGS LDSELSVSPK RNSISRTHKD KGPFHILSST SQTNKGPEGQ 

      2770       2780       2790       2800       2810 
SQAPASTSAS TRLFGLTKPK EKKEKKKKNK TSRSQPGDGP ASEVSAEGEE IFC 

« Hide

Isoform 2 [UniParc].

Checksum: 4EA29B253C76698E
Show »

2,817307,845
Isoform 3 [UniParc].

Checksum: 2E29FC699A72A6C5
Show »

1,429161,184