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Protein

Alpha-globin transcription factor CP2

Gene

TFCP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with UBP1 (By similarity). Functions as part of the SSP (stage selector protein) complex. Facilitates the interaction of the gamma-globin genes with enhancer elements contained in the locus control region in fetal erythroid cells. Interacts by binding to the stage selector element (SSE) in the proximal gamma-globin promoter.By similarity4 Publications

GO - Molecular functioni

  • DNA binding Source: ProtInc
  • protein C-terminus binding Source: UniProtKB
  • sequence-specific DNA binding Source: GO_Central
  • transcription factor activity, sequence-specific DNA binding Source: ProtInc

GO - Biological processi

  • regulation of transcription from RNA polymerase II promoter Source: ProtInc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

SIGNORiQ12800.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-globin transcription factor CP2
Alternative name(s):
SAA3 enhancer factor
Transcription factor LSF
Gene namesi
Name:TFCP2
Synonyms:LSF, SEF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:11748. TFCP2.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi211V → E: Does not affect DNA-binding activity. 1 Publication1
Mutagenesisi213I → R: Does not affect DNA-binding activity. 1 Publication1
Mutagenesisi234Q → L: Significant reduction of DNA-binding activity. 1 Publication1
Mutagenesisi236K → E: Significant reduction of DNA-binding activity. 1 Publication1

Organism-specific databases

DisGeNETi7024.
OpenTargetsiENSG00000135457.
PharmGKBiPA36463.

Polymorphism and mutation databases

BioMutaiTFCP2.
DMDMi90101767.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002280011 – 502Alpha-globin transcription factor CP2Add BLAST502

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei353PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ12800.
MaxQBiQ12800.
PaxDbiQ12800.
PeptideAtlasiQ12800.
PRIDEiQ12800.

PTM databases

iPTMnetiQ12800.
PhosphoSitePlusiQ12800.

Expressioni

Tissue specificityi

Ubiquitous. Expressed in brain, ovary, kidney, thymus, spleen, liver, adrenal, heart and lung (at protein level).3 Publications

Developmental stagei

Expressed in fetal erythroid tissue.2 Publications

Gene expression databases

BgeeiENSG00000135457.
CleanExiHS_TFCP2.
ExpressionAtlasiQ12800. baseline and differential.
GenevisibleiQ12800. HS.

Interactioni

Subunit structurei

Binds to DNA as a dimer, isoform 3 does not bind to DNA or affect the binding of isoform 1 to DNA. Interacts with UBP1 and PIAS1, and is probably part of a complex containing TFCP2, UBP1 and PIAS1 (By similarity). Component of the SSP (stage selector protein) complex, which appears to be a heteromer of TFCP2 and 2 copies of NFE4.By similarity2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein C-terminus binding Source: UniProtKB

Protein-protein interaction databases

BioGridi112882. 88 interactors.
IntActiQ12800. 100 interactors.
MINTiMINT-1388673.
STRINGi9606.ENSP00000257915.

Structurei

3D structure databases

ProteinModelPortaliQ12800.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni133 – 395DNA-bindingAdd BLAST263

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi314 – 319Poly-Pro6
Compositional biasi396 – 413Gln-richAdd BLAST18

Sequence similaritiesi

Belongs to the grh/CP2 family. CP2 subfamily.Curated

Phylogenomic databases

eggNOGiKOG4091. Eukaryota.
ENOG410XNZ6. LUCA.
GeneTreeiENSGT00760000119235.
HOGENOMiHOG000230625.
HOVERGENiHBG053805.
InParanoidiQ12800.
KOiK09275.
OMAiCQESQQA.
OrthoDBiEOG091G0YV2.
PhylomeDBiQ12800.
TreeFamiTF314132.

Family and domain databases

InterProiIPR007604. CP2.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF04516. CP2. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q12800-1) [UniParc]FASTAAdd to basket
Also known as: LBP-1c

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAWALKLPLA DEVIESGLVQ DFDASLSGIG QELGAGAYSM SDVLALPIFK
60 70 80 90 100
QEESSLPPDN ENKILPFQYV LCAATSPAVK LHDETLTYLN QGQSYEIRML
110 120 130 140 150
DNRKLGELPE INGKLVKSIF RVVFHDRRLQ YTEHQQLEGW RWNRPGDRIL
160 170 180 190 200
DIDIPMSVGI IDPRANPTQL NTVEFLWDPA KRTSVFIQVH CISTEFTMRK
210 220 230 240 250
HGGEKGVPFR VQIDTFKENE NGEYTEHLHS ASCQIKVFKP KGADRKQKTD
260 270 280 290 300
REKMEKRTPH EKEKYQPSYE TTILTECSPW PEITYVNNSP SPGFNSSHSS
310 320 330 340 350
FSLGEGNGSP NHQPEPPPPV TDNLLPTTTP QEAQQWLHRN RFSTFTRLFT
360 370 380 390 400
NFSGADLLKL TRDDVIQICG PADGIRLFNA LKGRMVRPRL TIYVCQESLQ
410 420 430 440 450
LREQQQQQQQ QQQKHEDGDS NGTFFVYHAI YLEELTAVEL TEKIAQLFSI
460 470 480 490 500
SPCQISQIYK QGPTGIHVLI SDEMIQNFQE EACFILDTMK AETNDSYHII

LK
Length:502
Mass (Da):57,256
Last modified:March 21, 2006 - v2
Checksum:iB93825C2687F89FF
GO
Isoform 2 (identifier: Q12800-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     189-239: Missing.
     491-491: Missing.

Show »
Length:450
Mass (Da):51,308
Checksum:i5004E9DF3925E852
GO
Isoform 3 (identifier: Q12800-3) [UniParc]FASTAAdd to basket
Also known as: LBP-1d

The sequence of this isoform differs from the canonical sequence as follows:
     189-239: Missing.

Show »
Length:451
Mass (Da):51,379
Checksum:i04DAF489CA939B17
GO
Isoform 4 (identifier: Q12800-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     491-491: Missing.

Show »
Length:501
Mass (Da):57,185
Checksum:i22D5A27DD537009D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92G → A in M84810 (PubMed:1732747).Curated1
Sequence conflicti92G → A AA sequence (PubMed:1732747).Curated1
Sequence conflicti249T → M in M84810 (PubMed:1732747).Curated1
Sequence conflicti249T → M AA sequence (PubMed:1732747).Curated1
Sequence conflicti491A → Q in AAA21324 (PubMed:8035790).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017647189 – 239Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST51
Alternative sequenceiVSP_017648491Missing in isoform 2 and isoform 4. 3 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84810 mRNA. No translation available.
U03494 mRNA. Translation: AAA21324.1.
U03495 mRNA. Translation: AAA21325.1.
AK291264 mRNA. Translation: BAF83953.1.
CH471111 Genomic DNA. Translation: EAW58178.1.
BC003634 mRNA. Translation: AAH03634.1.
CCDSiCCDS55827.1. [Q12800-2]
CCDS8808.1. [Q12800-1]
PIRiA42030.
A53771.
C56205.
RefSeqiNP_001166923.1. NM_001173452.1. [Q12800-4]
NP_001166924.1. NM_001173453.1. [Q12800-2]
NP_005644.2. NM_005653.4. [Q12800-1]
UniGeneiHs.48849.

Genome annotation databases

EnsembliENST00000257915; ENSP00000257915; ENSG00000135457. [Q12800-1]
ENST00000548115; ENSP00000447991; ENSG00000135457. [Q12800-2]
GeneIDi7024.
KEGGihsa:7024.
UCSCiuc001rxw.4. human. [Q12800-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84810 mRNA. No translation available.
U03494 mRNA. Translation: AAA21324.1.
U03495 mRNA. Translation: AAA21325.1.
AK291264 mRNA. Translation: BAF83953.1.
CH471111 Genomic DNA. Translation: EAW58178.1.
BC003634 mRNA. Translation: AAH03634.1.
CCDSiCCDS55827.1. [Q12800-2]
CCDS8808.1. [Q12800-1]
PIRiA42030.
A53771.
C56205.
RefSeqiNP_001166923.1. NM_001173452.1. [Q12800-4]
NP_001166924.1. NM_001173453.1. [Q12800-2]
NP_005644.2. NM_005653.4. [Q12800-1]
UniGeneiHs.48849.

3D structure databases

ProteinModelPortaliQ12800.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112882. 88 interactors.
IntActiQ12800. 100 interactors.
MINTiMINT-1388673.
STRINGi9606.ENSP00000257915.

PTM databases

iPTMnetiQ12800.
PhosphoSitePlusiQ12800.

Polymorphism and mutation databases

BioMutaiTFCP2.
DMDMi90101767.

Proteomic databases

EPDiQ12800.
MaxQBiQ12800.
PaxDbiQ12800.
PeptideAtlasiQ12800.
PRIDEiQ12800.

Protocols and materials databases

DNASUi7024.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000257915; ENSP00000257915; ENSG00000135457. [Q12800-1]
ENST00000548115; ENSP00000447991; ENSG00000135457. [Q12800-2]
GeneIDi7024.
KEGGihsa:7024.
UCSCiuc001rxw.4. human. [Q12800-1]

Organism-specific databases

CTDi7024.
DisGeNETi7024.
GeneCardsiTFCP2.
HGNCiHGNC:11748. TFCP2.
MIMi189889. gene.
neXtProtiNX_Q12800.
OpenTargetsiENSG00000135457.
PharmGKBiPA36463.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4091. Eukaryota.
ENOG410XNZ6. LUCA.
GeneTreeiENSGT00760000119235.
HOGENOMiHOG000230625.
HOVERGENiHBG053805.
InParanoidiQ12800.
KOiK09275.
OMAiCQESQQA.
OrthoDBiEOG091G0YV2.
PhylomeDBiQ12800.
TreeFamiTF314132.

Enzyme and pathway databases

SIGNORiQ12800.

Miscellaneous databases

ChiTaRSiTFCP2. human.
GeneWikiiTFCP2.
GenomeRNAii7024.
PROiQ12800.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000135457.
CleanExiHS_TFCP2.
ExpressionAtlasiQ12800. baseline and differential.
GenevisibleiQ12800. HS.

Family and domain databases

InterProiIPR007604. CP2.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF04516. CP2. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTFCP2_HUMAN
AccessioniPrimary (citable) accession number: Q12800
Secondary accession number(s): A8K5E9
, Q12801, Q9UD75, Q9UD77
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: March 21, 2006
Last modified: November 30, 2016
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

In PubMed:8114710 authors noted that a 10-fold molar excess of isoform 3 over isoform 1 inhibited DNA-binding.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.