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Q12800

- TFCP2_HUMAN

UniProt

Q12800 - TFCP2_HUMAN

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Protein
Alpha-globin transcription factor CP2
Gene
TFCP2, LSF, SEF
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with UBP1 By similarity. Functions as part of the SSP (stage selector protein) complex. Facilitates the interaction of the gamma-globin genes with enhancer elements contained in the locus control region in fetal erythroid cells. Interacts by binding to the stage selector element (SSE) in the proximal gamma-globin promoter.4 Publications

GO - Molecular functioni

  1. DNA binding Source: ProtInc
  2. sequence-specific DNA binding transcription factor activity Source: ProtInc
Complete GO annotation...

GO - Biological processi

  1. regulation of transcription from RNA polymerase II promoter Source: ProtInc
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-globin transcription factor CP2
Alternative name(s):
SAA3 enhancer factor
Transcription factor LSF
Gene namesi
Name:TFCP2
Synonyms:LSF, SEF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 12

Organism-specific databases

HGNCiHGNC:11748. TFCP2.

Subcellular locationi

Nucleus 3 Publications

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi211 – 2111V → E: Does not affect DNA-binding activity. 1 Publication
Mutagenesisi213 – 2131I → R: Does not affect DNA-binding activity. 1 Publication
Mutagenesisi234 – 2341Q → L: Significant reduction of DNA-binding activity. 1 Publication
Mutagenesisi236 – 2361K → E: Significant reduction of DNA-binding activity. 1 Publication

Organism-specific databases

PharmGKBiPA36463.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502Alpha-globin transcription factor CP2
PRO_0000228001Add
BLAST

Proteomic databases

MaxQBiQ12800.
PaxDbiQ12800.
PRIDEiQ12800.

PTM databases

PhosphoSiteiQ12800.

Expressioni

Tissue specificityi

Ubiquitous. Expressed in brain, ovary, kidney, thymus, spleen, liver, adrenal, heart and lung (at protein level).3 Publications

Developmental stagei

Expressed in fetal erythroid tissue.2 Publications

Gene expression databases

ArrayExpressiQ12800.
BgeeiQ12800.
CleanExiHS_TFCP2.
GenevestigatoriQ12800.

Interactioni

Subunit structurei

Binds to DNA as a dimer, isoform 3 does not bind to DNA or affect the binding of isoform 1 to DNA. Interacts with UBP1 and PIAS1, and is probably part of a complex containing TFCP2, UBP1 and PIAS1 By similarity. Component of the SSP (stage selector protein) complex, which appears to be a heteromer of TFCP2 and 2 copies of NFE4.2 Publications

Protein-protein interaction databases

BioGridi112882. 19 interactions.
IntActiQ12800. 38 interactions.
MINTiMINT-1388673.
STRINGi9606.ENSP00000257915.

Structurei

3D structure databases

ProteinModelPortaliQ12800.
SMRiQ12800. Positions 322-382.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni133 – 395263DNA-binding
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi314 – 3196Poly-Pro
Compositional biasi396 – 41318Gln-rich
Add
BLAST

Sequence similaritiesi

Belongs to the grh/CP2 family. CP2 subfamily.

Phylogenomic databases

eggNOGiNOG275458.
HOGENOMiHOG000230625.
HOVERGENiHBG053805.
InParanoidiQ12800.
KOiK09275.
OMAiDEVCFVL.
OrthoDBiEOG7FBRHB.
PhylomeDBiQ12800.
TreeFamiTF314132.

Family and domain databases

InterProiIPR007604. CP2.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF04516. CP2. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q12800-1) [UniParc]FASTAAdd to Basket

Also known as: LBP-1c

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAWALKLPLA DEVIESGLVQ DFDASLSGIG QELGAGAYSM SDVLALPIFK    50
QEESSLPPDN ENKILPFQYV LCAATSPAVK LHDETLTYLN QGQSYEIRML 100
DNRKLGELPE INGKLVKSIF RVVFHDRRLQ YTEHQQLEGW RWNRPGDRIL 150
DIDIPMSVGI IDPRANPTQL NTVEFLWDPA KRTSVFIQVH CISTEFTMRK 200
HGGEKGVPFR VQIDTFKENE NGEYTEHLHS ASCQIKVFKP KGADRKQKTD 250
REKMEKRTPH EKEKYQPSYE TTILTECSPW PEITYVNNSP SPGFNSSHSS 300
FSLGEGNGSP NHQPEPPPPV TDNLLPTTTP QEAQQWLHRN RFSTFTRLFT 350
NFSGADLLKL TRDDVIQICG PADGIRLFNA LKGRMVRPRL TIYVCQESLQ 400
LREQQQQQQQ QQQKHEDGDS NGTFFVYHAI YLEELTAVEL TEKIAQLFSI 450
SPCQISQIYK QGPTGIHVLI SDEMIQNFQE EACFILDTMK AETNDSYHII 500
LK 502
Length:502
Mass (Da):57,256
Last modified:March 21, 2006 - v2
Checksum:iB93825C2687F89FF
GO
Isoform 2 (identifier: Q12800-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     189-239: Missing.
     491-491: Missing.

Show »
Length:450
Mass (Da):51,308
Checksum:i5004E9DF3925E852
GO
Isoform 3 (identifier: Q12800-3) [UniParc]FASTAAdd to Basket

Also known as: LBP-1d

The sequence of this isoform differs from the canonical sequence as follows:
     189-239: Missing.

Show »
Length:451
Mass (Da):51,379
Checksum:i04DAF489CA939B17
GO
Isoform 4 (identifier: Q12800-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     491-491: Missing.

Show »
Length:501
Mass (Da):57,185
Checksum:i22D5A27DD537009D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei189 – 23951Missing in isoform 2 and isoform 3.
VSP_017647Add
BLAST
Alternative sequencei491 – 4911Missing in isoform 2 and isoform 4.
VSP_017648

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti92 – 921G → A in M84810. 1 Publication
Sequence conflicti92 – 921G → A AA sequence 1 Publication
Sequence conflicti249 – 2491T → M in M84810. 1 Publication
Sequence conflicti249 – 2491T → M AA sequence 1 Publication
Sequence conflicti491 – 4911A → Q in AAA21324. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84810 mRNA. No translation available.
U03494 mRNA. Translation: AAA21324.1.
U03495 mRNA. Translation: AAA21325.1.
AK291264 mRNA. Translation: BAF83953.1.
CH471111 Genomic DNA. Translation: EAW58178.1.
BC003634 mRNA. Translation: AAH03634.1.
CCDSiCCDS55827.1. [Q12800-2]
CCDS8808.1. [Q12800-1]
PIRiA42030.
A53771.
C56205.
RefSeqiNP_001166923.1. NM_001173452.1. [Q12800-4]
NP_001166924.1. NM_001173453.1. [Q12800-2]
NP_005644.2. NM_005653.4. [Q12800-1]
UniGeneiHs.48849.

Genome annotation databases

EnsembliENST00000257915; ENSP00000257915; ENSG00000135457. [Q12800-1]
ENST00000307660; ENSP00000304411; ENSG00000135457. [Q12800-3]
ENST00000548115; ENSP00000447991; ENSG00000135457. [Q12800-2]
GeneIDi7024.
KEGGihsa:7024.
UCSCiuc001rxv.2. human. [Q12800-4]
uc001rxw.3. human. [Q12800-1]
uc009zlx.2. human. [Q12800-2]

Polymorphism databases

DMDMi90101767.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84810 mRNA. No translation available.
U03494 mRNA. Translation: AAA21324.1 .
U03495 mRNA. Translation: AAA21325.1 .
AK291264 mRNA. Translation: BAF83953.1 .
CH471111 Genomic DNA. Translation: EAW58178.1 .
BC003634 mRNA. Translation: AAH03634.1 .
CCDSi CCDS55827.1. [Q12800-2 ]
CCDS8808.1. [Q12800-1 ]
PIRi A42030.
A53771.
C56205.
RefSeqi NP_001166923.1. NM_001173452.1. [Q12800-4 ]
NP_001166924.1. NM_001173453.1. [Q12800-2 ]
NP_005644.2. NM_005653.4. [Q12800-1 ]
UniGenei Hs.48849.

3D structure databases

ProteinModelPortali Q12800.
SMRi Q12800. Positions 322-382.
ModBasei Search...

Protein-protein interaction databases

BioGridi 112882. 19 interactions.
IntActi Q12800. 38 interactions.
MINTi MINT-1388673.
STRINGi 9606.ENSP00000257915.

PTM databases

PhosphoSitei Q12800.

Polymorphism databases

DMDMi 90101767.

Proteomic databases

MaxQBi Q12800.
PaxDbi Q12800.
PRIDEi Q12800.

Protocols and materials databases

DNASUi 7024.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000257915 ; ENSP00000257915 ; ENSG00000135457 . [Q12800-1 ]
ENST00000307660 ; ENSP00000304411 ; ENSG00000135457 . [Q12800-3 ]
ENST00000548115 ; ENSP00000447991 ; ENSG00000135457 . [Q12800-2 ]
GeneIDi 7024.
KEGGi hsa:7024.
UCSCi uc001rxv.2. human. [Q12800-4 ]
uc001rxw.3. human. [Q12800-1 ]
uc009zlx.2. human. [Q12800-2 ]

Organism-specific databases

CTDi 7024.
GeneCardsi GC12M051488.
HGNCi HGNC:11748. TFCP2.
MIMi 189889. gene.
neXtProti NX_Q12800.
PharmGKBi PA36463.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG275458.
HOGENOMi HOG000230625.
HOVERGENi HBG053805.
InParanoidi Q12800.
KOi K09275.
OMAi DEVCFVL.
OrthoDBi EOG7FBRHB.
PhylomeDBi Q12800.
TreeFami TF314132.

Miscellaneous databases

GeneWikii TFCP2.
GenomeRNAii 7024.
NextBioi 27443.
PROi Q12800.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q12800.
Bgeei Q12800.
CleanExi HS_TFCP2.
Genevestigatori Q12800.

Family and domain databases

InterProi IPR007604. CP2.
IPR013761. SAM/pointed.
[Graphical view ]
Pfami PF04516. CP2. 1 hit.
[Graphical view ]
SUPFAMi SSF47769. SSF47769. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the alpha-globin transcription factor CP2."
    Lim L.C., Swendeman S.L., Sheffery M.
    Mol. Cell. Biol. 12:828-835(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), PROTEIN SEQUENCE OF 129-140; 165-176; 348-358 AND 390-397, FUNCTION, SUBCELLULAR LOCATION.
  2. "Characterization of a family of related cellular transcription factors which can modulate human immunodeficiency virus type 1 transcription in vitro."
    Yoon J.-B., Li G., Roeder R.G.
    Mol. Cell. Biol. 14:1776-1785(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [MRNA] OF 61-502 (ISOFORM 3), DNA-BINDING, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING.
  3. "One exon of the human LSF gene includes conserved regions involved in novel DNA-binding and dimerization motifs."
    Shirra M.K., Zhu Q., Huang H.-C., Pallas D., Hansen U.
    Mol. Cell. Biol. 14:5076-5087(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PROTEIN SEQUENCE OF 7-21; 81-98; 149-164; 165-179; 348-359 AND 363-376, FUNCTION, ALTERNATIVE SPLICING, MUTAGENESIS OF VAL-211; ILE-213; GLN-234 AND LYS-236.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
    Tissue: Teratocarcinoma.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Muscle.
  7. "Characterization of the genomic structure, chromosomal location, promoter, and development expression of the alpha-globin transcription factor CP2."
    Swendeman S.L., Spielholz C., Jenkins N.A., Gilbert D.J., Copeland N.G., Sheffery M.
    J. Biol. Chem. 269:11663-11671(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  8. "Hemoglobin switching in man and chicken is mediated by a heteromeric complex between the ubiquitous transcription factor CP2 and a developmentally specific protein."
    Jane S.M., Nienhuis A.W., Cunningham J.M.
    EMBO J. 14:97-105(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE SSP COMPLEX, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  9. Erratum
    Jane S.M., Nienhuis A.W., Cunningham J.M.
    EMBO J. 15:854-855(1995)
  10. "Purification and characterization of the serum amyloid A3 enhancer factor."
    Bing Z., Reddy S.A., Ren Y., Qin J., Liao W.S.-L.
    J. Biol. Chem. 274:24649-24656(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  11. "Induction of human fetal globin gene expression by a novel erythroid factor, NF-E4."
    Zhou W., Clouston D.R., Wang X., Cerruti L., Cunningham J.M., Jane S.M.
    Mol. Cell. Biol. 20:7662-7672(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NFE4.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTFCP2_HUMAN
AccessioniPrimary (citable) accession number: Q12800
Secondary accession number(s): A8K5E9
, Q12801, Q9UD75, Q9UD77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: March 21, 2006
Last modified: July 9, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

In 1 Publication authors noted that a 10-fold molar excess of isoform 3 over isoform 1 inhibited DNA-binding.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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