Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q12800

- TFCP2_HUMAN

UniProt

Q12800 - TFCP2_HUMAN

Protein

Alpha-globin transcription factor CP2

Gene

TFCP2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (21 Mar 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with UBP1 By similarity. Functions as part of the SSP (stage selector protein) complex. Facilitates the interaction of the gamma-globin genes with enhancer elements contained in the locus control region in fetal erythroid cells. Interacts by binding to the stage selector element (SSE) in the proximal gamma-globin promoter.By similarity4 Publications

    GO - Molecular functioni

    1. DNA binding Source: ProtInc
    2. sequence-specific DNA binding transcription factor activity Source: ProtInc

    GO - Biological processi

    1. regulation of transcription from RNA polymerase II promoter Source: ProtInc
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-globin transcription factor CP2
    Alternative name(s):
    SAA3 enhancer factor
    Transcription factor LSF
    Gene namesi
    Name:TFCP2
    Synonyms:LSF, SEF
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:11748. TFCP2.

    Subcellular locationi

    Nucleus 3 Publications

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi211 – 2111V → E: Does not affect DNA-binding activity. 1 Publication
    Mutagenesisi213 – 2131I → R: Does not affect DNA-binding activity. 1 Publication
    Mutagenesisi234 – 2341Q → L: Significant reduction of DNA-binding activity. 1 Publication
    Mutagenesisi236 – 2361K → E: Significant reduction of DNA-binding activity. 1 Publication

    Organism-specific databases

    PharmGKBiPA36463.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 502502Alpha-globin transcription factor CP2PRO_0000228001Add
    BLAST

    Proteomic databases

    MaxQBiQ12800.
    PaxDbiQ12800.
    PRIDEiQ12800.

    PTM databases

    PhosphoSiteiQ12800.

    Expressioni

    Tissue specificityi

    Ubiquitous. Expressed in brain, ovary, kidney, thymus, spleen, liver, adrenal, heart and lung (at protein level).3 Publications

    Developmental stagei

    Expressed in fetal erythroid tissue.2 Publications

    Gene expression databases

    ArrayExpressiQ12800.
    BgeeiQ12800.
    CleanExiHS_TFCP2.
    GenevestigatoriQ12800.

    Interactioni

    Subunit structurei

    Binds to DNA as a dimer, isoform 3 does not bind to DNA or affect the binding of isoform 1 to DNA. Interacts with UBP1 and PIAS1, and is probably part of a complex containing TFCP2, UBP1 and PIAS1 By similarity. Component of the SSP (stage selector protein) complex, which appears to be a heteromer of TFCP2 and 2 copies of NFE4.By similarity2 Publications

    Protein-protein interaction databases

    BioGridi112882. 19 interactions.
    IntActiQ12800. 38 interactions.
    MINTiMINT-1388673.
    STRINGi9606.ENSP00000257915.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12800.
    SMRiQ12800. Positions 322-382.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni133 – 395263DNA-bindingAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi314 – 3196Poly-Pro
    Compositional biasi396 – 41318Gln-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the grh/CP2 family. CP2 subfamily.Curated

    Phylogenomic databases

    eggNOGiNOG275458.
    HOGENOMiHOG000230625.
    HOVERGENiHBG053805.
    InParanoidiQ12800.
    KOiK09275.
    OMAiDEVCFVL.
    OrthoDBiEOG7FBRHB.
    PhylomeDBiQ12800.
    TreeFamiTF314132.

    Family and domain databases

    InterProiIPR007604. CP2.
    IPR013761. SAM/pointed.
    [Graphical view]
    PfamiPF04516. CP2. 1 hit.
    [Graphical view]
    SUPFAMiSSF47769. SSF47769. 1 hit.

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q12800-1) [UniParc]FASTAAdd to Basket

    Also known as: LBP-1c

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAWALKLPLA DEVIESGLVQ DFDASLSGIG QELGAGAYSM SDVLALPIFK    50
    QEESSLPPDN ENKILPFQYV LCAATSPAVK LHDETLTYLN QGQSYEIRML 100
    DNRKLGELPE INGKLVKSIF RVVFHDRRLQ YTEHQQLEGW RWNRPGDRIL 150
    DIDIPMSVGI IDPRANPTQL NTVEFLWDPA KRTSVFIQVH CISTEFTMRK 200
    HGGEKGVPFR VQIDTFKENE NGEYTEHLHS ASCQIKVFKP KGADRKQKTD 250
    REKMEKRTPH EKEKYQPSYE TTILTECSPW PEITYVNNSP SPGFNSSHSS 300
    FSLGEGNGSP NHQPEPPPPV TDNLLPTTTP QEAQQWLHRN RFSTFTRLFT 350
    NFSGADLLKL TRDDVIQICG PADGIRLFNA LKGRMVRPRL TIYVCQESLQ 400
    LREQQQQQQQ QQQKHEDGDS NGTFFVYHAI YLEELTAVEL TEKIAQLFSI 450
    SPCQISQIYK QGPTGIHVLI SDEMIQNFQE EACFILDTMK AETNDSYHII 500
    LK 502
    Length:502
    Mass (Da):57,256
    Last modified:March 21, 2006 - v2
    Checksum:iB93825C2687F89FF
    GO
    Isoform 2 (identifier: Q12800-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         189-239: Missing.
         491-491: Missing.

    Show »
    Length:450
    Mass (Da):51,308
    Checksum:i5004E9DF3925E852
    GO
    Isoform 3 (identifier: Q12800-3) [UniParc]FASTAAdd to Basket

    Also known as: LBP-1d

    The sequence of this isoform differs from the canonical sequence as follows:
         189-239: Missing.

    Show »
    Length:451
    Mass (Da):51,379
    Checksum:i04DAF489CA939B17
    GO
    Isoform 4 (identifier: Q12800-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         491-491: Missing.

    Show »
    Length:501
    Mass (Da):57,185
    Checksum:i22D5A27DD537009D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti92 – 921G → A in M84810. (PubMed:1732747)Curated
    Sequence conflicti92 – 921G → A AA sequence (PubMed:1732747)Curated
    Sequence conflicti249 – 2491T → M in M84810. (PubMed:1732747)Curated
    Sequence conflicti249 – 2491T → M AA sequence (PubMed:1732747)Curated
    Sequence conflicti491 – 4911A → Q in AAA21324. (PubMed:8035790)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei189 – 23951Missing in isoform 2 and isoform 3. 2 PublicationsVSP_017647Add
    BLAST
    Alternative sequencei491 – 4911Missing in isoform 2 and isoform 4. 3 PublicationsVSP_017648

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84810 mRNA. No translation available.
    U03494 mRNA. Translation: AAA21324.1.
    U03495 mRNA. Translation: AAA21325.1.
    AK291264 mRNA. Translation: BAF83953.1.
    CH471111 Genomic DNA. Translation: EAW58178.1.
    BC003634 mRNA. Translation: AAH03634.1.
    CCDSiCCDS55827.1. [Q12800-2]
    CCDS8808.1. [Q12800-1]
    PIRiA42030.
    A53771.
    C56205.
    RefSeqiNP_001166923.1. NM_001173452.1. [Q12800-4]
    NP_001166924.1. NM_001173453.1. [Q12800-2]
    NP_005644.2. NM_005653.4. [Q12800-1]
    UniGeneiHs.48849.

    Genome annotation databases

    EnsembliENST00000257915; ENSP00000257915; ENSG00000135457. [Q12800-1]
    ENST00000548115; ENSP00000447991; ENSG00000135457. [Q12800-2]
    GeneIDi7024.
    KEGGihsa:7024.
    UCSCiuc001rxv.2. human. [Q12800-4]
    uc001rxw.3. human. [Q12800-1]
    uc009zlx.2. human. [Q12800-2]

    Polymorphism databases

    DMDMi90101767.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84810 mRNA. No translation available.
    U03494 mRNA. Translation: AAA21324.1 .
    U03495 mRNA. Translation: AAA21325.1 .
    AK291264 mRNA. Translation: BAF83953.1 .
    CH471111 Genomic DNA. Translation: EAW58178.1 .
    BC003634 mRNA. Translation: AAH03634.1 .
    CCDSi CCDS55827.1. [Q12800-2 ]
    CCDS8808.1. [Q12800-1 ]
    PIRi A42030.
    A53771.
    C56205.
    RefSeqi NP_001166923.1. NM_001173452.1. [Q12800-4 ]
    NP_001166924.1. NM_001173453.1. [Q12800-2 ]
    NP_005644.2. NM_005653.4. [Q12800-1 ]
    UniGenei Hs.48849.

    3D structure databases

    ProteinModelPortali Q12800.
    SMRi Q12800. Positions 322-382.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112882. 19 interactions.
    IntActi Q12800. 38 interactions.
    MINTi MINT-1388673.
    STRINGi 9606.ENSP00000257915.

    PTM databases

    PhosphoSitei Q12800.

    Polymorphism databases

    DMDMi 90101767.

    Proteomic databases

    MaxQBi Q12800.
    PaxDbi Q12800.
    PRIDEi Q12800.

    Protocols and materials databases

    DNASUi 7024.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000257915 ; ENSP00000257915 ; ENSG00000135457 . [Q12800-1 ]
    ENST00000548115 ; ENSP00000447991 ; ENSG00000135457 . [Q12800-2 ]
    GeneIDi 7024.
    KEGGi hsa:7024.
    UCSCi uc001rxv.2. human. [Q12800-4 ]
    uc001rxw.3. human. [Q12800-1 ]
    uc009zlx.2. human. [Q12800-2 ]

    Organism-specific databases

    CTDi 7024.
    GeneCardsi GC12M051488.
    HGNCi HGNC:11748. TFCP2.
    MIMi 189889. gene.
    neXtProti NX_Q12800.
    PharmGKBi PA36463.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG275458.
    HOGENOMi HOG000230625.
    HOVERGENi HBG053805.
    InParanoidi Q12800.
    KOi K09275.
    OMAi DEVCFVL.
    OrthoDBi EOG7FBRHB.
    PhylomeDBi Q12800.
    TreeFami TF314132.

    Miscellaneous databases

    GeneWikii TFCP2.
    GenomeRNAii 7024.
    NextBioi 27443.
    PROi Q12800.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q12800.
    Bgeei Q12800.
    CleanExi HS_TFCP2.
    Genevestigatori Q12800.

    Family and domain databases

    InterProi IPR007604. CP2.
    IPR013761. SAM/pointed.
    [Graphical view ]
    Pfami PF04516. CP2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47769. SSF47769. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the alpha-globin transcription factor CP2."
      Lim L.C., Swendeman S.L., Sheffery M.
      Mol. Cell. Biol. 12:828-835(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), PROTEIN SEQUENCE OF 129-140; 165-176; 348-358 AND 390-397, FUNCTION, SUBCELLULAR LOCATION.
    2. "Characterization of a family of related cellular transcription factors which can modulate human immunodeficiency virus type 1 transcription in vitro."
      Yoon J.-B., Li G., Roeder R.G.
      Mol. Cell. Biol. 14:1776-1785(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [MRNA] OF 61-502 (ISOFORM 3), DNA-BINDING, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING.
    3. "One exon of the human LSF gene includes conserved regions involved in novel DNA-binding and dimerization motifs."
      Shirra M.K., Zhu Q., Huang H.-C., Pallas D., Hansen U.
      Mol. Cell. Biol. 14:5076-5087(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PROTEIN SEQUENCE OF 7-21; 81-98; 149-164; 165-179; 348-359 AND 363-376, FUNCTION, ALTERNATIVE SPLICING, MUTAGENESIS OF VAL-211; ILE-213; GLN-234 AND LYS-236.
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
      Tissue: Teratocarcinoma.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Muscle.
    7. "Characterization of the genomic structure, chromosomal location, promoter, and development expression of the alpha-globin transcription factor CP2."
      Swendeman S.L., Spielholz C., Jenkins N.A., Gilbert D.J., Copeland N.G., Sheffery M.
      J. Biol. Chem. 269:11663-11671(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    8. "Hemoglobin switching in man and chicken is mediated by a heteromeric complex between the ubiquitous transcription factor CP2 and a developmentally specific protein."
      Jane S.M., Nienhuis A.W., Cunningham J.M.
      EMBO J. 14:97-105(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE SSP COMPLEX, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    9. Erratum
      Jane S.M., Nienhuis A.W., Cunningham J.M.
      EMBO J. 15:854-855(1995)
    10. "Purification and characterization of the serum amyloid A3 enhancer factor."
      Bing Z., Reddy S.A., Ren Y., Qin J., Liao W.S.-L.
      J. Biol. Chem. 274:24649-24656(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    11. "Induction of human fetal globin gene expression by a novel erythroid factor, NF-E4."
      Zhou W., Clouston D.R., Wang X., Cerruti L., Cunningham J.M., Jane S.M.
      Mol. Cell. Biol. 20:7662-7672(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NFE4.
    12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTFCP2_HUMAN
    AccessioniPrimary (citable) accession number: Q12800
    Secondary accession number(s): A8K5E9
    , Q12801, Q9UD75, Q9UD77
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 21, 2006
    Last sequence update: March 21, 2006
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    In PubMed:8114710 authors noted that a 10-fold molar excess of isoform 3 over isoform 1 inhibited DNA-binding.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3