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Q127W8

- PNP_POLSJ

UniProt

Q127W8 - PNP_POLSJ

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Protein
Polyribonucleotide nucleotidyltransferase
Gene
pnp, Bpro_3260
Organism
Polaromonas sp. (strain JS666 / ATCC BAA-500)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi488 – 4881Magnesium By similarity
Metal bindingi494 – 4941Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. RNA processing Source: InterPro
  2. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciPSP296591:GHI4-3981-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:Bpro_3260
OrganismiPolaromonas sp. (strain JS666 / ATCC BAA-500)
Taxonomic identifieri296591 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaePolaromonas
ProteomesiUP000001983: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 707707Polyribonucleotide nucleotidyltransferaseUniRule annotation
PRO_0000329760Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi296591.Bpro_3260.

Structurei

3D structure databases

ProteinModelPortaliQ127W8.
SMRiQ127W8. Positions 619-692.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini555 – 61460KH
Add
BLAST
Domaini624 – 69269S1 motif
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiEYIINPD.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q127W8-1 [UniParc]FASTAAdd to Basket

« Hide

MSIFNKVTKS FQWGQHKVTM ETGEVARQAS GAVVVDMDGT VVLATVVAKT    50
DAKPGQDFFP LTVDYLEKTY AAGRIPGSFF KREGRPSEFE TLTSRLIDRP 100
IRPLFPEGFF NEVQVVIHVL SLNPEVEGDI PALIASSAAL SISGIPFNGP 150
IGAARVAYVD GQYVLNPGKT QLKDSKMDLV VAGTEAAVLM VESEAQQLSE 200
EIMLGAVVFG HEQANIAINA IHELVRDAGK PVWDWQAPAK DEPLIAKVNE 250
LAGAKLQAAY QIRSKQARTQ ACRVAYADVM AALKADGVAF DGVTVEGMLF 300
DIEAKIVRSQ ILAGEPRIDG RDTRTVRAIE IRNSVLPRTH GSALFTRGET 350
QALVVTTLGT ERDAQRIDAL SGDYEDRFML HYNMPPFATG ETGRVGSPKR 400
REIGHGRLAK RALIAVLPTK EEFPYTMRVV SEITESNGSS SMASVCGGCL 450
SLMDAGVPMK AHVAGIAMGL IKEENRFAVL TDILGDEDHL GDMDFKVAGT 500
TFGITALQMD IKIQGITKEI MQVALAQAKE ARMHILGKMQ EAMGEAKAEV 550
SDFAPRLYVM KINPEKIRDV IGKGGAVIRA LTEETGTQIN IEEDGTITIA 600
SNDSAKADEA KRRIAEITAE VEIGKVYEGA ITKILDFGAL VNLLPGKDGL 650
LHISQIAHER VEKVTDYLSE GQIVKVKVLE TDEKGRVKLS MKALLDRPAQ 700
NQDQDRG 707
Length:707
Mass (Da):76,722
Last modified:August 22, 2006 - v1
Checksum:i09994DE7BF681774
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000316 Genomic DNA. Translation: ABE45174.1.
RefSeqiWP_011484169.1. NC_007948.1.
YP_550072.1. NC_007948.1.

Genome annotation databases

EnsemblBacteriaiABE45174; ABE45174; Bpro_3260.
GeneIDi4014069.
KEGGipol:Bpro_3260.
PATRICi22959742. VBIPolSp102244_3325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000316 Genomic DNA. Translation: ABE45174.1 .
RefSeqi WP_011484169.1. NC_007948.1.
YP_550072.1. NC_007948.1.

3D structure databases

ProteinModelPortali Q127W8.
SMRi Q127W8. Positions 619-692.
ModBasei Search...

Protein-protein interaction databases

STRINGi 296591.Bpro_3260.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABE45174 ; ABE45174 ; Bpro_3260 .
GeneIDi 4014069.
KEGGi pol:Bpro_3260.
PATRICi 22959742. VBIPolSp102244_3325.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218327.
KOi K00962.
OMAi EYIINPD.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci PSP296591:GHI4-3981-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JS666 / ATCC BAA-500.

Entry informationi

Entry nameiPNP_POLSJ
AccessioniPrimary (citable) accession number: Q127W8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: August 22, 2006
Last modified: September 3, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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