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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Polaromonas sp. (strain JS666 / ATCC BAA-500)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi488 – 4881MagnesiumUniRule annotation
Metal bindingi494 – 4941MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciPSP296591:GHI4-3981-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Bpro_3260
OrganismiPolaromonas sp. (strain JS666 / ATCC BAA-500)
Taxonomic identifieri296591 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaePolaromonas
ProteomesiUP000001983 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 707707Polyribonucleotide nucleotidyltransferasePRO_0000329760Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi296591.Bpro_3260.

Structurei

3D structure databases

ProteinModelPortaliQ127W8.
SMRiQ127W8. Positions 619-692.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini555 – 61460KHUniRule annotationAdd
BLAST
Domaini624 – 69269S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q127W8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIFNKVTKS FQWGQHKVTM ETGEVARQAS GAVVVDMDGT VVLATVVAKT
60 70 80 90 100
DAKPGQDFFP LTVDYLEKTY AAGRIPGSFF KREGRPSEFE TLTSRLIDRP
110 120 130 140 150
IRPLFPEGFF NEVQVVIHVL SLNPEVEGDI PALIASSAAL SISGIPFNGP
160 170 180 190 200
IGAARVAYVD GQYVLNPGKT QLKDSKMDLV VAGTEAAVLM VESEAQQLSE
210 220 230 240 250
EIMLGAVVFG HEQANIAINA IHELVRDAGK PVWDWQAPAK DEPLIAKVNE
260 270 280 290 300
LAGAKLQAAY QIRSKQARTQ ACRVAYADVM AALKADGVAF DGVTVEGMLF
310 320 330 340 350
DIEAKIVRSQ ILAGEPRIDG RDTRTVRAIE IRNSVLPRTH GSALFTRGET
360 370 380 390 400
QALVVTTLGT ERDAQRIDAL SGDYEDRFML HYNMPPFATG ETGRVGSPKR
410 420 430 440 450
REIGHGRLAK RALIAVLPTK EEFPYTMRVV SEITESNGSS SMASVCGGCL
460 470 480 490 500
SLMDAGVPMK AHVAGIAMGL IKEENRFAVL TDILGDEDHL GDMDFKVAGT
510 520 530 540 550
TFGITALQMD IKIQGITKEI MQVALAQAKE ARMHILGKMQ EAMGEAKAEV
560 570 580 590 600
SDFAPRLYVM KINPEKIRDV IGKGGAVIRA LTEETGTQIN IEEDGTITIA
610 620 630 640 650
SNDSAKADEA KRRIAEITAE VEIGKVYEGA ITKILDFGAL VNLLPGKDGL
660 670 680 690 700
LHISQIAHER VEKVTDYLSE GQIVKVKVLE TDEKGRVKLS MKALLDRPAQ

NQDQDRG
Length:707
Mass (Da):76,722
Last modified:August 22, 2006 - v1
Checksum:i09994DE7BF681774
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000316 Genomic DNA. Translation: ABE45174.1.
RefSeqiWP_011484169.1. NC_007948.1.

Genome annotation databases

EnsemblBacteriaiABE45174; ABE45174; Bpro_3260.
KEGGipol:Bpro_3260.
PATRICi22959742. VBIPolSp102244_3325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000316 Genomic DNA. Translation: ABE45174.1.
RefSeqiWP_011484169.1. NC_007948.1.

3D structure databases

ProteinModelPortaliQ127W8.
SMRiQ127W8. Positions 619-692.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi296591.Bpro_3260.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABE45174; ABE45174; Bpro_3260.
KEGGipol:Bpro_3260.
PATRICi22959742. VBIPolSp102244_3325.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciPSP296591:GHI4-3981-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JS666 / ATCC BAA-500.

Entry informationi

Entry nameiPNP_POLSJ
AccessioniPrimary (citable) accession number: Q127W8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: August 22, 2006
Last modified: July 22, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.