Q127N2 (Q127N2_POLSJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase HAMAP MF_00126
EC=6.1.1.18 HAMAP MF_00126

Alternative name(s):
Glutaminyl-tRNA synthetase HAMAP MF_00126

Gene names

Name:	glnS HAMAP MF_00126
Ordered Locus Names:	Bpro_3350

Organism

Polaromonas sp. (strain JS666 / ATCC BAA-500) [Complete proteome] [HAMAP]

Taxonomic identifier

296591 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Polaromonas

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126 SAAS SAAS020059
Subunit structure	Monomer By similarity. HAMAP MF_00126 SAAS SAAS020059
Subcellular location	Cytoplasm By similarity HAMAP MF_00126.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP MF_00126 RuleBase RU003489

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_00126 SAAS SAAS004514 RuleBase RU003489
Cellular component	Cytoplasm HAMAP MF_00126 SAAS SAAS020059
Ligand	ATP-binding HAMAP MF_00126 RuleBase RU003489 SAAS SAAS004514 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP MF_00126 RuleBase RU003489 SAAS SAAS004514 EMBL ABE45260.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	75 – 85	11	"HIGH" region By similarity HAMAP MF_00126
	Motif	322 – 326	5	"KMSKS" region By similarity HAMAP MF_00126
Sites
	Binding site	325	1	ATP By similarity HAMAP MF_00126

Sequences

	Sequence		Length	Mass (Da)
	Q127N2 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 4ED670F6D2AF31E8	FASTA	612	68,875
	10 20 30 40 50 60 MTSPAPSKAG PKGTAAAAPA DKENHKPSNF LRQIIEKDLA EGTYATRRWG GSPGDAAHHA 70 80 90 100 110 120 SGQPDPARVR LRFPPEPNGY LHIGHAKSIY LNFGLARDYG GVCHLRFDDT NPEKEDTEYV 130 140 150 160 170 180 SSIIEAVKWL GFDWENNGPS GNECNLYQAS DYFDFMYRAA EYLIESGNAY VDEQTAEEMR 190 200 210 220 230 240 LSRGDFGHPG TNSPFRSRTP EQNLARFREM RDGLHEDGSM VLRAKIDMAS PNINMRDPAI 250 260 270 280 290 300 YRIRRAHHHN TGDKWCIYPM YTFAHPIEDA LEQITHSICT LEFEDQRPFY DWLLDRLCEG 310 320 330 340 350 360 GLVATPHPHQ YEFARLNLTY VLTSKRKLAQ LVYDHKVTGW DDPRMPTIVG LRRRGYTPEA 370 380 390 400 410 420 LHLFAERIGV TKSDSWIDYS TLEGALRDTL DPIAPRAMAV LDPVKLVLTN WDTVMGAGKL 430 440 450 460 470 480 DDCSAPVHPH HPELGKRSFK IGKEVWIERT DFEETPPKGF FRLFPGNKVR LKYGHVIECT 490 500 510 520 530 540 GATRDANGQV TEVQATLIPD TKSGTPGSDA IKVKGVITWV GVNDAVQAEV RLYDRLFKEA 550 560 570 580 590 600 HPGTGEKDFM EELNPDSLKV VTAYVEPSLA SAPAGQRFQF ERHGYFVTDI VDHKPGKTVF 610 NRVTGMKDSW GK

References

[1]

"Complete sequence of chromosome of Polaromonas sp. JS666." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Munk A.C., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JS666 / ATCC BAA-500.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000316 Genomic DNA. Translation: ABE45260.1.
RefSeq	YP_550158.1. NC_007948.1.
3D structure databases
ProteinModelPortal	Q127N2.
SMR	Q127N2. Positions 28-609.
ModBase	Search...
Protein-protein interaction databases
STRING	Q127N2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4013964.
GenomeReviews	Gene locus Bpro_3350 in contig CP000316_GR.
KEGG	pol:Bpro_3350.
NMPDR	fig|296591.1.peg.1290.
PATRIC	22959924. VBIPolSp102244_3416.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG334108.
OMA	RMPTIAG.
PhylomeDB	Q127N2.
ProtClustDB	PRK05347.
Enzyme and pathway databases
BioCyc	PSP296591:BPRO_3350-MONOMER.
Family and domain databases
HAMAP	MF_00126. Gln_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ib. IPR022861. Gln_tRNA_synth_bac. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 2 hits. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01886.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. GlnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q127N2_POLSJ

Accession

Primary (citable) accession number: Q127N2

Entry history

Integrated into UniProtKB/TrEMBL:	August 22, 2006
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)