ID   PNRC1_HUMAN             Reviewed;         327 AA.
AC   Q12796; B2R6Q0; E1P515; Q5T7J6; Q7Z5N0;
DT   06-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   24-JAN-2024, entry version 159.
DE   RecName: Full=Proline-rich nuclear receptor coactivator 1;
DE   AltName: Full=Proline-rich protein 2;
DE   AltName: Full=Protein B4-2;
GN   Name=PNRC1; Synonyms=PROL2;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX   PubMed=7578250; DOI=10.1016/0167-4781(95)00159-e;
RA   Chen J., Liu L., Pohajdak B.;
RT   "Cloning a cDNA from human NK/T cells which codes for a protein with high
RT   proline content.";
RL   Biochim. Biophys. Acta 1264:19-22(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA   Zhou G., Ke R., Li H., Cao L., Shen C., Zheng G., Yu R., Zhong G.,
RA   Huang F., Lin L., Yang S.;
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=14574404; DOI=10.1038/nature02055;
RA   Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA   Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA   Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA   Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA   Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA   Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA   Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA   Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA   Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA   Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA   Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA   French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA   Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA   Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA   Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA   Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA   Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA   Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA   Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA   Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA   Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA   Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA   Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA   Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA   Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA   Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA   Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA   West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA   Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA   Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA   Rogers J., Beck S.;
RT   "The DNA sequence and analysis of human chromosome 6.";
RL   Nature 425:805-811(2003).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Skin;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [7]
RP   FUNCTION, INTERACTION WITH NUCLEAR RECEPTORS, MUTAGENESIS OF PRO-287 AND
RP   PRO-290, NUCLEAR LOCALIZATION SIGNAL, AND SUBCELLULAR LOCATION.
RX   PubMed=10894149; DOI=10.1210/mend.14.7.0480;
RA   Zhou D., Quach K.M., Yang C., Lee S.Y., Pohajdak B., Chen S.;
RT   "PNRC: a proline-rich nuclear receptor coregulatory protein that modulates
RT   transcriptional activation of multiple nuclear receptors including orphan
RT   receptors SF1 (steroidogenic factor 1) and ERRalpha1 (estrogen related
RT   receptor alpha-1).";
RL   Mol. Endocrinol. 14:986-998(2000).
RN   [8]
RP   TISSUE SPECIFICITY, AND INTERACTION WITH GRB2.
RX   PubMed=11574675; DOI=10.1093/nar/29.19.3939;
RA   Zhou D., Chen S.;
RT   "PNRC2 is a 16 kDa coactivator that interacts with nuclear receptors
RT   through an SH3-binding motif.";
RL   Nucleic Acids Res. 29:3939-3948(2001).
CC   -!- FUNCTION: Nuclear receptor coactivator. May play a role in signal
CC       transduction. {ECO:0000269|PubMed:10894149}.
CC   -!- SUBUNIT: Interacts with many nuclear receptors including AR, ESR1,
CC       ESRRA, ESRRG, NR3C1/GR, NR5A1, PGR, TR, RAR and RXR. Interacts with
CC       GRB2. {ECO:0000269|PubMed:10894149, ECO:0000269|PubMed:11574675}.
CC   -!- INTERACTION:
CC       Q12796; Q9H1D9: POLR3F; NbExp=3; IntAct=EBI-2827376, EBI-710067;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q12796-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q12796-2; Sequence=VSP_055501;
CC   -!- TISSUE SPECIFICITY: Expressed in liver, lung, fat and NK/T cells.
CC       {ECO:0000269|PubMed:11574675}.
CC   -!- DOMAIN: The interaction between PNRC1 and nuclear receptors is
CC       dependent on the SH3 binding motif.
CC   -!- SIMILARITY: Belongs to the PNRC family. PNRC1 subfamily. {ECO:0000305}.
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DR   EMBL; U03105; AAA85576.1; -; mRNA.
DR   EMBL; AY303779; AAP76184.1; -; mRNA.
DR   EMBL; AK312665; BAG35547.1; -; mRNA.
DR   EMBL; AL353135; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471051; EAW48563.1; -; Genomic_DNA.
DR   EMBL; CH471051; EAW48564.1; -; Genomic_DNA.
DR   EMBL; BC018112; AAH18112.1; -; mRNA.
DR   CCDS; CCDS5018.1; -. [Q12796-1]
DR   PIR; S59960; S59960.
DR   RefSeq; NP_006804.1; NM_006813.2. [Q12796-1]
DR   AlphaFoldDB; Q12796; -.
DR   SMR; Q12796; -.
DR   BioGRID; 116157; 21.
DR   IntAct; Q12796; 11.
DR   MINT; Q12796; -.
DR   STRING; 9606.ENSP00000336931; -.
DR   GlyGen; Q12796; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; Q12796; -.
DR   PhosphoSitePlus; Q12796; -.
DR   BioMuta; PNRC1; -.
DR   DMDM; 21362754; -.
DR   EPD; Q12796; -.
DR   jPOST; Q12796; -.
DR   MassIVE; Q12796; -.
DR   PaxDb; 9606-ENSP00000336931; -.
DR   PeptideAtlas; Q12796; -.
DR   ProteomicsDB; 58943; -. [Q12796-1]
DR   Antibodypedia; 31849; 48 antibodies from 19 providers.
DR   DNASU; 10957; -.
DR   Ensembl; ENST00000336032.4; ENSP00000336931.3; ENSG00000146278.11. [Q12796-1]
DR   GeneID; 10957; -.
DR   KEGG; hsa:10957; -.
DR   MANE-Select; ENST00000336032.4; ENSP00000336931.3; NM_006813.3; NP_006804.1.
DR   UCSC; uc003pmv.4; human. [Q12796-1]
DR   AGR; HGNC:17278; -.
DR   CTD; 10957; -.
DR   DisGeNET; 10957; -.
DR   GeneCards; PNRC1; -.
DR   HGNC; HGNC:17278; PNRC1.
DR   HPA; ENSG00000146278; Low tissue specificity.
DR   MIM; 606714; gene.
DR   neXtProt; NX_Q12796; -.
DR   OpenTargets; ENSG00000146278; -.
DR   PharmGKB; PA33804; -.
DR   VEuPathDB; HostDB:ENSG00000146278; -.
DR   eggNOG; ENOG502RYBG; Eukaryota.
DR   GeneTree; ENSGT00530000063881; -.
DR   HOGENOM; CLU_086541_0_0_1; -.
DR   InParanoid; Q12796; -.
DR   OMA; WMGTTIE; -.
DR   OrthoDB; 5319967at2759; -.
DR   PhylomeDB; Q12796; -.
DR   TreeFam; TF333211; -.
DR   PathwayCommons; Q12796; -.
DR   SignaLink; Q12796; -.
DR   BioGRID-ORCS; 10957; 16 hits in 1170 CRISPR screens.
DR   ChiTaRS; PNRC1; human.
DR   GeneWiki; PNRC1; -.
DR   GenomeRNAi; 10957; -.
DR   Pharos; Q12796; Tbio.
DR   PRO; PR:Q12796; -.
DR   Proteomes; UP000005640; Chromosome 6.
DR   RNAct; Q12796; Protein.
DR   Bgee; ENSG00000146278; Expressed in calcaneal tendon and 203 other cell types or tissues.
DR   ExpressionAtlas; Q12796; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000932; C:P-body; IBA:GO_Central.
DR   GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IBA:GO_Central.
DR   InterPro; IPR026780; PNRC1/2.
DR   PANTHER; PTHR15405; PROLINE-RICH NUCLEAR RECEPTOR COACTIVATOR; 1.
DR   PANTHER; PTHR15405:SF4; PROLINE-RICH NUCLEAR RECEPTOR COACTIVATOR 1; 1.
DR   Pfam; PF15365; PNRC; 1.
DR   Genevisible; Q12796; HS.
PE   1: Evidence at protein level;
KW   Activator; Alternative splicing; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..327
FT                   /note="Proline-rich nuclear receptor coactivator 1"
FT                   /id="PRO_0000058482"
FT   REGION          66..175
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          280..300
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           94..101
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000269|PubMed:10894149"
FT   MOTIF           285..291
FT                   /note="SH3-binding"
FT   COMPBIAS        111..125
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         102..180
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.2"
FT                   /id="VSP_055501"
FT   VARIANT         252
FT                   /note="P -> L (in dbSNP:rs2231277)"
FT                   /id="VAR_051284"
FT   MUTAGEN         287
FT                   /note="P->A: Abolishes the interaction with the nuclear
FT                   receptors; when associated with A-290."
FT                   /evidence="ECO:0000269|PubMed:10894149"
FT   MUTAGEN         290
FT                   /note="P->A: Abolishes the interaction with the nuclear
FT                   receptors; when associated with A-287."
FT                   /evidence="ECO:0000269|PubMed:10894149"
FT   CONFLICT        218
FT                   /note="Y -> N (in Ref. 2; AAP76184)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   327 AA;  35225 MW;  97E0D65C2E690763 CRC64;
     MTVVSVPQRE PLVLGGRLAP LGFSSRGYFG ALPMVTTAPP PLPRIPDPRA LPPTLFLPHF
     LGGDGPCLTP QPRAPAALPN RSLAVAGGTP RAAPKKRRKK KVRASPAGQL PSRFHQYQQH
     RPSLEGGRSP ATGPSGAQEV PGPAAALAPS PAAAAGTEGA SPDLAPLRPA APGQTPLRKE
     VLKSKMGKSE KIALPHGQLV HGIHLYEQPK INRQKSKYNL PLTKITSAKR NENNFWQDSV
     SSDRIQKQEK KPFKNTENIK NSHLKKSAFL TEVSQKENYA GAKFSDPPSP SVLPKPPSHW
     MGSTVENSNQ NRELMAVHLK TLLKVQT
//