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Protein

Twinfilin-1

Gene

TWF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (By similarity).By similarity

GO - Molecular functioni

  • actin monomer binding Source: BHF-UCL
  • ATP binding Source: BHF-UCL
  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • phosphatidylinositol-4,5-bisphosphate binding Source: BHF-UCL

GO - Biological processi

  • barbed-end actin filament capping Source: Ensembl
  • negative regulation of actin filament polymerization Source: BHF-UCL
  • regulation of actin phosphorylation Source: BHF-UCL
  • sequestering of actin monomers Source: BHF-UCL
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000151239-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Twinfilin-1
Alternative name(s):
Protein A6
Protein tyrosine kinase 9
Gene namesi
Name:TWF1
Synonyms:PTK9
OrganismiHomo sapiens (Human)Imported
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:9620. TWF1.

Subcellular locationi

  • Cytoplasm
  • Cytoplasmcytoskeleton By similarity

  • Note: Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts (By similarity).By similarity

GO - Cellular componenti

  • actin cytoskeleton Source: UniProtKB
  • cell-cell adherens junction Source: BHF-UCL
  • cell-cell junction Source: BHF-UCL
  • cytoplasm Source: HPA
  • filopodium Source: BHF-UCL
  • focal adhesion Source: UniProtKB
  • myofibril Source: BHF-UCL
  • perinuclear region of cytoplasm Source: BHF-UCL
  • ruffle membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Organism-specific databases

DisGeNETi5756.
OpenTargetsiENSG00000151239.
PharmGKBiPA162407406.

Polymorphism and mutation databases

BioMutaiTWF1.
DMDMi259016376.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002149502 – 350Twinfilin-1Add BLAST349

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei143PhosphoserineCombined sources1
Modified residuei277PhosphoserineBy similarity1
Modified residuei309PhosphotyrosineCombined sources1
Modified residuei349PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylated on serine and threonine residues.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ12792.
PaxDbiQ12792.
PeptideAtlasiQ12792.
PRIDEiQ12792.

2D gel databases

OGPiQ12792.

PTM databases

iPTMnetiQ12792.
PhosphoSitePlusiQ12792.

Expressioni

Tissue specificityi

Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver.1 Publication

Gene expression databases

BgeeiENSG00000151239.
ExpressionAtlasiQ12792. baseline and differential.
GenevisibleiQ12792. HS.

Organism-specific databases

HPAiHPA018116.

Interactioni

Subunit structurei

Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated (By similarity).By similarity

GO - Molecular functioni

  • actin monomer binding Source: BHF-UCL
  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL

Protein-protein interaction databases

BioGridi111723. 58 interactors.
IntActiQ12792. 28 interactors.
MINTiMINT-3026525.
STRINGi9606.ENSP00000449428.

Structurei

3D structure databases

ProteinModelPortaliQ12792.
SMRiQ12792.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 139ADF-H 1PROSITE-ProRule annotationAdd BLAST138
Domaini175 – 313ADF-H 2PROSITE-ProRule annotationAdd BLAST139

Sequence similaritiesi

Contains 2 ADF-H domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1747. Eukaryota.
ENOG410XS1J. LUCA.
GeneTreeiENSGT00530000063868.
HOVERGENiHBG000848.
InParanoidiQ12792.
KOiK08870.
OMAiYDYLQFR.
OrthoDBiEOG091G0BWK.
PhylomeDBiQ12792.
TreeFamiTF352598.

Family and domain databases

Gene3Di3.40.20.10. 2 hits.
InterProiIPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR028458. Twinfilin.
[Graphical view]
PANTHERiPTHR13759. PTHR13759. 1 hit.
PfamiPF00241. Cofilin_ADF. 2 hits.
[Graphical view]
SMARTiSM00102. ADF. 2 hits.
[Graphical view]
PROSITEiPS51263. ADF_H. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1Curated (identifier: Q12792-2) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSHQTGIQAS EDVKEIFARA RNGKYRLLKI SIENEQLVIG SYSQPSDSWD
60 70 80 90 100
KDYDSFVLPL LEDKQPCYIL FRLDSQNAQG YEWIFIAWSP DHSHVRQKML
110 120 130 140 150
YAATRATLKK EFGGGHIKDE VFGTVKEDVS LHGYKKYLLS QSSPAPLTAA
160 170 180 190 200
EEELRQIKIN EVQTDVGVDT KHQTLQGVAF PISREAFQAL EKLNNRQLNY
210 220 230 240 250
VQLEIDIKNE IIILANTTNT ELKDLPKRIP KDSARYHFFL YKHSHEGDYL
260 270 280 290 300
ESIVFIYSMP GYTCSIRERM LYSSCKSRLL EIVERQLQMD VIRKIEIDNG
310 320 330 340 350
DELTADFLYE EVHPKQHAHK QSFAKPKGPA GKRGIRRLIR GPAETEATTD
Length:350
Mass (Da):40,283
Last modified:September 22, 2009 - v3
Checksum:i5F68A6946E969A80
GO
Isoform 3 (identifier: Q12792-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     161-161: E → ESPEDHIG

Show »
Length:357
Mass (Da):41,018
Checksum:i826E95A7D995033B
GO
Isoform 4 (identifier: Q12792-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-98: Missing.

Show »
Length:252
Mass (Da):28,823
Checksum:iCCE0A5A82BBE109D
GO

Sequence cautioni

The sequence AAH43148 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH68548 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAD92539 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12D → Y in CAG46561 (Ref. 3) Curated1
Sequence conflicti246E → V in BAG54588 (PubMed:14702039).Curated1
Sequence conflicti269R → W in CAG46536 (Ref. 3) Curated1
Sequence conflicti302E → G in BAF83912 (PubMed:14702039).Curated1
Sequence conflicti329P → H in BAG59691 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0380751 – 98Missing in isoform 4. 2 PublicationsAdd BLAST98
Alternative sequenceiVSP_017899161E → ESPEDHIG in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02680 mRNA. Translation: AAC50062.1.
AK127868 mRNA. Translation: BAG54588.1.
AK291223 mRNA. Translation: BAF83912.1.
AK297206 mRNA. Translation: BAG59691.1.
CR541736 mRNA. Translation: CAG46536.1.
CR541761 mRNA. Translation: CAG46561.1.
BT019691 mRNA. Translation: AAV38497.1.
AB209302 mRNA. Translation: BAD92539.1. Different initiation.
BC022344 mRNA. Translation: AAH22344.1.
BC043148 mRNA. Translation: AAH43148.2. Different initiation.
BC068548 mRNA. Translation: AAH68548.1. Different initiation.
CCDSiCCDS31780.2. [Q12792-2]
CCDS55818.1. [Q12792-3]
PIRiA55922.
RefSeqiNP_001229326.1. NM_001242397.1. [Q12792-3]
NP_002813.3. NM_002822.4. [Q12792-2]
UniGeneiHs.189075.
Hs.313056.

Genome annotation databases

EnsembliENST00000395510; ENSP00000378886; ENSG00000151239. [Q12792-2]
ENST00000548315; ENSP00000449428; ENSG00000151239. [Q12792-3]
ENST00000552521; ENSP00000448750; ENSG00000151239. [Q12792-4]
GeneIDi5756.
KEGGihsa:5756.
UCSCiuc001roa.4. human. [Q12792-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Protein Spotlight

Molecular embrace - Issue 73 of August 2006

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02680 mRNA. Translation: AAC50062.1.
AK127868 mRNA. Translation: BAG54588.1.
AK291223 mRNA. Translation: BAF83912.1.
AK297206 mRNA. Translation: BAG59691.1.
CR541736 mRNA. Translation: CAG46536.1.
CR541761 mRNA. Translation: CAG46561.1.
BT019691 mRNA. Translation: AAV38497.1.
AB209302 mRNA. Translation: BAD92539.1. Different initiation.
BC022344 mRNA. Translation: AAH22344.1.
BC043148 mRNA. Translation: AAH43148.2. Different initiation.
BC068548 mRNA. Translation: AAH68548.1. Different initiation.
CCDSiCCDS31780.2. [Q12792-2]
CCDS55818.1. [Q12792-3]
PIRiA55922.
RefSeqiNP_001229326.1. NM_001242397.1. [Q12792-3]
NP_002813.3. NM_002822.4. [Q12792-2]
UniGeneiHs.189075.
Hs.313056.

3D structure databases

ProteinModelPortaliQ12792.
SMRiQ12792.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111723. 58 interactors.
IntActiQ12792. 28 interactors.
MINTiMINT-3026525.
STRINGi9606.ENSP00000449428.

PTM databases

iPTMnetiQ12792.
PhosphoSitePlusiQ12792.

Polymorphism and mutation databases

BioMutaiTWF1.
DMDMi259016376.

2D gel databases

OGPiQ12792.

Proteomic databases

EPDiQ12792.
PaxDbiQ12792.
PeptideAtlasiQ12792.
PRIDEiQ12792.

Protocols and materials databases

DNASUi5756.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000395510; ENSP00000378886; ENSG00000151239. [Q12792-2]
ENST00000548315; ENSP00000449428; ENSG00000151239. [Q12792-3]
ENST00000552521; ENSP00000448750; ENSG00000151239. [Q12792-4]
GeneIDi5756.
KEGGihsa:5756.
UCSCiuc001roa.4. human. [Q12792-2]

Organism-specific databases

CTDi5756.
DisGeNETi5756.
GeneCardsiTWF1.
HGNCiHGNC:9620. TWF1.
HPAiHPA018116.
MIMi610932. gene.
neXtProtiNX_Q12792.
OpenTargetsiENSG00000151239.
PharmGKBiPA162407406.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1747. Eukaryota.
ENOG410XS1J. LUCA.
GeneTreeiENSGT00530000063868.
HOVERGENiHBG000848.
InParanoidiQ12792.
KOiK08870.
OMAiYDYLQFR.
OrthoDBiEOG091G0BWK.
PhylomeDBiQ12792.
TreeFamiTF352598.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000151239-MONOMER.

Miscellaneous databases

GeneWikiiTWF1.
GenomeRNAii5756.
PROiQ12792.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000151239.
ExpressionAtlasiQ12792. baseline and differential.
GenevisibleiQ12792. HS.

Family and domain databases

Gene3Di3.40.20.10. 2 hits.
InterProiIPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR028458. Twinfilin.
[Graphical view]
PANTHERiPTHR13759. PTHR13759. 1 hit.
PfamiPF00241. Cofilin_ADF. 2 hits.
[Graphical view]
SMARTiSM00102. ADF. 2 hits.
[Graphical view]
PROSITEiPS51263. ADF_H. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTWF1_HUMAN
AccessioniPrimary (citable) accession number: Q12792
Secondary accession number(s): A8K5A8
, B3KXS6, B4DLX9, Q59G07, Q5U0B1, Q6FHJ1, Q6FHL6, Q6NUK9, Q86XL6, Q8TCD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: September 22, 2009
Last modified: November 2, 2016
This is version 153 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was originally thought to have protein tyrosine kinase activity.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.