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Protein

Rho guanine nucleotide exchange factor 5

Gene

ARHGEF5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. GTP binding Source: ProtInc
  2. Rho guanyl-nucleotide exchange factor activity Source: InterPro

GO - Biological processi

  1. actin cytoskeleton organization Source: Ensembl
  2. intracellular signal transduction Source: InterPro
  3. myeloid dendritic cell chemotaxis Source: Ensembl
  4. positive regulation of podosome assembly Source: Ensembl
  5. regulation of GTPase activity Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Rho guanine nucleotide exchange factor 5
Alternative name(s):
Ephexin-3
Guanine nucleotide regulatory protein TIM
Oncogene TIM
Transforming immortalized mammary oncogene
p60 TIM
Gene namesi
Name:ARHGEF5
Synonyms:TIM
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:13209. ARHGEF5.

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

PharmGKBiPA24975.

Polymorphism and mutation databases

BioMutaiARHGEF5.
DMDMi290457679.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15971597Rho guanine nucleotide exchange factor 5PRO_0000080916Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei184 – 1841Phosphoserine1 Publication
Modified residuei445 – 4451Phosphoserine3 Publications
Modified residuei450 – 4501Phosphoserine4 Publications
Modified residuei1126 – 11261Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12774.
PaxDbiQ12774.
PRIDEiQ12774.

PTM databases

PhosphoSiteiQ12774.

Expressioni

Tissue specificityi

Mainly expressed in kidney, liver, pancreas, lung and placenta.

Gene expression databases

BgeeiQ12774.
CleanExiHS_ARHGEF5.
ExpressionAtlasiQ12774. baseline and differential.
GenevestigatoriQ12774.

Organism-specific databases

HPAiHPA020980.
HPA029504.
HPA044188.
HPA045619.
HPA045699.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CALCOCO2Q131373EBI-602199,EBI-739580
EFHC2Q5JST63EBI-602199,EBI-2349927
GRB2P629934EBI-602199,EBI-401755
HSPBAP1Q96EW23EBI-602199,EBI-720457
TRIM27P143733EBI-602199,EBI-719493
TRIM54Q9BYV23EBI-602199,EBI-2130429

Protein-protein interaction databases

BioGridi113696. 19 interactions.
IntActiQ12774. 18 interactions.
MINTiMINT-3974855.
STRINGi9606.ENSP00000056217.

Structurei

3D structure databases

ProteinModelPortaliQ12774.
SMRiQ12774. Positions 1168-1506, 1513-1572.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1174 – 1358185DHPROSITE-ProRule annotationAdd
BLAST
Domaini1390 – 1502113PHPROSITE-ProRule annotationAdd
BLAST
Domaini1513 – 156957SH3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiCOG5422.
GeneTreeiENSGT00550000074493.
HOGENOMiHOG000170375.
HOVERGENiHBG060842.
InParanoidiQ12774.
OMAiQARQPEK.
OrthoDBiEOG7NPFSZ.
PhylomeDBiQ12774.
TreeFamiTF342609.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR029212. ARHGEF5/35_N.
IPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF15441. ARHGEF5_35. 1 hit.
PF00621. RhoGEF. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q12774-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAEEAQRGA SPPISAIEEF SIIPEAPMRS SQVSALGLEA QEDEDPSYKW
60 70 80 90 100
REEHRLSATQ QSELRDVCDY AIETMPSFPK EGSADVEPNQ ESLVAEACDT
110 120 130 140 150
PEHWEAVPQS LAGRQARTLA PPELWACPIQ SEHLDMAPFS SDLGSEEEEV
160 170 180 190 200
EFWPGLTSLT LGSGQAEEEE ETSSDNSGQT RYYSPCEEHP AETNQNEGSE
210 220 230 240 250
SGTIRQGEEL PPEELQESQG LLHPQEVQVL EEQGQQEAGF RGEGTLREDV
260 270 280 290 300
CADGLLGEEQ MIEQVNDEKG EQKQKQEQVQ DVMLGRQGER MGLTGEPEGL
310 320 330 340 350
NDGEWEQEDM ERKAQGQGGP EQGEERKREL QVPEENRADS QDEKSQTFLG
360 370 380 390 400
KSEEVTGKQE DHGIKEKGVP VSGQEAKEPE SWDGGRLGAV GRARSREEEN
410 420 430 440 450
EHHGPSMPAL IAPEDSPHCD LFPGASYLMT QIPGTQTESR AEELSPAALS
460 470 480 490 500
PSLEPIRCSH QPISLLGSFL TEESPDKEID QNSQQEESRL RKGTVSSQGT
510 520 530 540 550
EVVFASASVT PPRTPDSAPP SPAEAYPITP ASVSARPPVA FPRRETSCAA
560 570 580 590 600
RAPETASAPL SMDDPSPCGT SEMCPAALYG FPSTGTSPPR PPANSTGTVQ
610 620 630 640 650
HLRSDSFPGS HRTEQTPDLV GMLLSYSHSE LPQRPPKPAI YSSVTPRRDR
660 670 680 690 700
RSGRDYSTVS ASPTALSTLK QDSQESISNL ERPSSPPSIQ PWVSPHNPAF
710 720 730 740 750
ATESPAYGSS PSFVSMEDVR IHEPLPPPPP QRRDTHPSVV ETDGHARVVV
760 770 780 790 800
PTLKQHSHPP PLALGSGLHA PHKGPLPQAS DPAVARQHRP LPSTPDSSHH
810 820 830 840 850
AQATPRWRYN KPLPPTPDLP QPHLPPISAP GSSRIYRPLP PLPIIDPPTE
860 870 880 890 900
PPPLPPKSRG RSRSTRGGHM NSGGHAKTRP ACQDWTVPLP ASAGRTSWPP
910 920 930 940 950
ATARSTESFT STSRSKSEVS PGMAFSNMTN FLCPSSPTTP WTPELQGPTS
960 970 980 990 1000
KDEAGVSEHP EAPAREPLRR TTPQQGASGP GRSPVGQARQ PEKPSHLHLE
1010 1020 1030 1040 1050
KASSWPHRRD SGRPPGDSSG QAVAPSEGAN KHKGWSRQGL RRPSILPEGS
1060 1070 1080 1090 1100
SDSRGPAVEK HPGPSDTVVF REKKPKEVMG GFSRRCSKLI NSSQLLYQEY
1110 1120 1130 1140 1150
SDVVLNKEIQ SQQRLESLSE TPGPSSPRQP RKALVSSESY LQRLSMASSG
1160 1170 1180 1190 1200
SLWQEIPVVR NSTVLLSMTH EDQKLQEVKF ELIVSEASYL RSLNIAVDHF
1210 1220 1230 1240 1250
QLSTSLRATL SNQEHQWLFS RLQDVRDVSA TFLSDLEENF ENNIFSFQVC
1260 1270 1280 1290 1300
DVVLNHAPDF RRVYLPYVTN QTYQERTFQS LMNSNSNFRE VLEKLESDPV
1310 1320 1330 1340 1350
CQRLSLKSFL ILPFQRITRL KLLLQNILKR TQPGSSEEAE ATKAHHALEQ
1360 1370 1380 1390 1400
LIRDCNNNVQ SMRRTEELIY LSQKIEFECK IFPLISQSRW LVKSGELTAL
1410 1420 1430 1440 1450
EFSASPGLRR KLNTRPVHLH LFNDCLLLSR PREGSRFLVF DHAPFSSIRG
1460 1470 1480 1490 1500
EKCEMKLHGP HKNLFRLFLR QNTQGAQAEF LFRTETQSEK LRWISALAMP
1510 1520 1530 1540 1550
REELDLLECY NSPQVQCLRA YKPRENDELA LEKADVVMVT QQSSDGWLEG
1560 1570 1580 1590
VRLSDGERGW FPVQQVEFIS NPEVRAQNLK EAHRVKTAKL QLVEQQA
Length:1,597
Mass (Da):176,799
Last modified:March 2, 2010 - v3
Checksum:i93EC1B1D7E1198C5
GO
Isoform 2 (identifier: Q12774-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1078: Missing.

Show »
Length:519
Mass (Da):60,056
Checksum:i2EE2C16B3F42C053
GO

Sequence cautioni

The sequence BAD18708.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti487 – 4871E → G in BAD18708 (Ref. 2) Curated
Sequence conflicti575 – 5751P → Q in BAD18708 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 10781078Missing in isoform 2. 2 PublicationsVSP_035175Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02082 mRNA. Translation: AAA18010.1.
AK160365 mRNA. Translation: BAD18708.1. Different initiation.
AC004534 Genomic DNA. Translation: AAC12958.1.
CH471146 Genomic DNA. Translation: EAW80096.1.
BC010046 mRNA. Translation: AAH10046.1.
BC011839 mRNA. Translation: AAH11839.1.
BC014555 mRNA. Translation: AAH14555.1.
CCDSiCCDS34771.1. [Q12774-1]
PIRiI38402.
RefSeqiNP_005426.2. NM_005435.3. [Q12774-1]
UniGeneiHs.334.
Hs.534621.

Genome annotation databases

EnsembliENST00000056217; ENSP00000056217; ENSG00000050327. [Q12774-1]
ENST00000471847; ENSP00000418227; ENSG00000050327. [Q12774-2]
GeneIDi7984.
KEGGihsa:7984.
UCSCiuc003wek.3. human. [Q12774-1]

Polymorphism and mutation databases

BioMutaiARHGEF5.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02082 mRNA. Translation: AAA18010.1.
AK160365 mRNA. Translation: BAD18708.1. Different initiation.
AC004534 Genomic DNA. Translation: AAC12958.1.
CH471146 Genomic DNA. Translation: EAW80096.1.
BC010046 mRNA. Translation: AAH10046.1.
BC011839 mRNA. Translation: AAH11839.1.
BC014555 mRNA. Translation: AAH14555.1.
CCDSiCCDS34771.1. [Q12774-1]
PIRiI38402.
RefSeqiNP_005426.2. NM_005435.3. [Q12774-1]
UniGeneiHs.334.
Hs.534621.

3D structure databases

ProteinModelPortaliQ12774.
SMRiQ12774. Positions 1168-1506, 1513-1572.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113696. 19 interactions.
IntActiQ12774. 18 interactions.
MINTiMINT-3974855.
STRINGi9606.ENSP00000056217.

PTM databases

PhosphoSiteiQ12774.

Polymorphism and mutation databases

BioMutaiARHGEF5.
DMDMi290457679.

Proteomic databases

MaxQBiQ12774.
PaxDbiQ12774.
PRIDEiQ12774.

Protocols and materials databases

DNASUi7984.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000056217; ENSP00000056217; ENSG00000050327. [Q12774-1]
ENST00000471847; ENSP00000418227; ENSG00000050327. [Q12774-2]
GeneIDi7984.
KEGGihsa:7984.
UCSCiuc003wek.3. human. [Q12774-1]

Organism-specific databases

CTDi7984.
GeneCardsiGC07P144052.
HGNCiHGNC:13209. ARHGEF5.
HPAiHPA020980.
HPA029504.
HPA044188.
HPA045619.
HPA045699.
MIMi600888. gene.
neXtProtiNX_Q12774.
PharmGKBiPA24975.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5422.
GeneTreeiENSGT00550000074493.
HOGENOMiHOG000170375.
HOVERGENiHBG060842.
InParanoidiQ12774.
OMAiQARQPEK.
OrthoDBiEOG7NPFSZ.
PhylomeDBiQ12774.
TreeFamiTF342609.

Miscellaneous databases

GeneWikiiARHGEF5.
GenomeRNAii7984.
NextBioi30518.
PROiQ12774.
SOURCEiSearch...

Gene expression databases

BgeeiQ12774.
CleanExiHS_ARHGEF5.
ExpressionAtlasiQ12774. baseline and differential.
GenevestigatoriQ12774.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR029212. ARHGEF5/35_N.
IPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF15441. ARHGEF5_35. 1 hit.
PF00621. RhoGEF. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression cDNA cloning of a novel oncogene with sequence similarity to regulators of small GTP-binding proteins."
    Chan A.M.-L., McGovern E.S., Catalano G., Fleming T.P., Miki T.
    Oncogene 9:1057-1063(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Epithelium.
  2. "The nucleotide sequence of a long cDNA clone isolated from human spleen."
    Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O.
    Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Spleen.
  3. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Placenta and Skin.
  6. Cited for: NOMENCLATURE.
  7. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
    Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
    Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-445 AND SER-450, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-184; SER-445; SER-450 AND SER-1126, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-445; SER-450 AND SER-1126, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-450, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiARHG5_HUMAN
AccessioniPrimary (citable) accession number: Q12774
Secondary accession number(s): A6NNJ2, Q6ZML7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: March 2, 2010
Last modified: April 29, 2015
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.