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Q12774 (ARHG5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rho guanine nucleotide exchange factor 5
Alternative name(s):
Ephexin-3
Guanine nucleotide regulatory protein TIM
Oncogene TIM
Transforming immortalized mammary oncogene
p60 TIM
Gene names
Name:ARHGEF5
Synonyms:TIM
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1597 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Tissue specificity

Mainly expressed in kidney, liver, pancreas, lung and placenta.

Sequence similarities

Contains 1 DH (DBL-homology) domain.

Contains 1 PH domain.

Contains 1 SH3 domain.

Sequence caution

The sequence BAD18708.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

GRB2P629934EBI-602199,EBI-401755

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q12774-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q12774-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1078: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15971597Rho guanine nucleotide exchange factor 5
PRO_0000080916

Regions

Domain1174 – 1358185DH
Domain1390 – 1502113PH
Domain1513 – 156957SH3

Amino acid modifications

Modified residue1841Phosphoserine Ref.8
Modified residue4451Phosphoserine Ref.7 Ref.8 Ref.9
Modified residue4501Phosphoserine Ref.7 Ref.8 Ref.9
Modified residue11261Phosphoserine Ref.8 Ref.9

Natural variations

Alternative sequence1 – 10781078Missing in isoform 2.
VSP_035175

Experimental info

Sequence conflict4871E → G in BAD18708. Ref.2
Sequence conflict5751P → Q in BAD18708. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 2, 2010. Version 3.
Checksum: 93EC1B1D7E1198C5

FASTA1,597176,799
        10         20         30         40         50         60 
MEAEEAQRGA SPPISAIEEF SIIPEAPMRS SQVSALGLEA QEDEDPSYKW REEHRLSATQ 

        70         80         90        100        110        120 
QSELRDVCDY AIETMPSFPK EGSADVEPNQ ESLVAEACDT PEHWEAVPQS LAGRQARTLA 

       130        140        150        160        170        180 
PPELWACPIQ SEHLDMAPFS SDLGSEEEEV EFWPGLTSLT LGSGQAEEEE ETSSDNSGQT 

       190        200        210        220        230        240 
RYYSPCEEHP AETNQNEGSE SGTIRQGEEL PPEELQESQG LLHPQEVQVL EEQGQQEAGF 

       250        260        270        280        290        300 
RGEGTLREDV CADGLLGEEQ MIEQVNDEKG EQKQKQEQVQ DVMLGRQGER MGLTGEPEGL 

       310        320        330        340        350        360 
NDGEWEQEDM ERKAQGQGGP EQGEERKREL QVPEENRADS QDEKSQTFLG KSEEVTGKQE 

       370        380        390        400        410        420 
DHGIKEKGVP VSGQEAKEPE SWDGGRLGAV GRARSREEEN EHHGPSMPAL IAPEDSPHCD 

       430        440        450        460        470        480 
LFPGASYLMT QIPGTQTESR AEELSPAALS PSLEPIRCSH QPISLLGSFL TEESPDKEID 

       490        500        510        520        530        540 
QNSQQEESRL RKGTVSSQGT EVVFASASVT PPRTPDSAPP SPAEAYPITP ASVSARPPVA 

       550        560        570        580        590        600 
FPRRETSCAA RAPETASAPL SMDDPSPCGT SEMCPAALYG FPSTGTSPPR PPANSTGTVQ 

       610        620        630        640        650        660 
HLRSDSFPGS HRTEQTPDLV GMLLSYSHSE LPQRPPKPAI YSSVTPRRDR RSGRDYSTVS 

       670        680        690        700        710        720 
ASPTALSTLK QDSQESISNL ERPSSPPSIQ PWVSPHNPAF ATESPAYGSS PSFVSMEDVR 

       730        740        750        760        770        780 
IHEPLPPPPP QRRDTHPSVV ETDGHARVVV PTLKQHSHPP PLALGSGLHA PHKGPLPQAS 

       790        800        810        820        830        840 
DPAVARQHRP LPSTPDSSHH AQATPRWRYN KPLPPTPDLP QPHLPPISAP GSSRIYRPLP 

       850        860        870        880        890        900 
PLPIIDPPTE PPPLPPKSRG RSRSTRGGHM NSGGHAKTRP ACQDWTVPLP ASAGRTSWPP 

       910        920        930        940        950        960 
ATARSTESFT STSRSKSEVS PGMAFSNMTN FLCPSSPTTP WTPELQGPTS KDEAGVSEHP 

       970        980        990       1000       1010       1020 
EAPAREPLRR TTPQQGASGP GRSPVGQARQ PEKPSHLHLE KASSWPHRRD SGRPPGDSSG 

      1030       1040       1050       1060       1070       1080 
QAVAPSEGAN KHKGWSRQGL RRPSILPEGS SDSRGPAVEK HPGPSDTVVF REKKPKEVMG 

      1090       1100       1110       1120       1130       1140 
GFSRRCSKLI NSSQLLYQEY SDVVLNKEIQ SQQRLESLSE TPGPSSPRQP RKALVSSESY 

      1150       1160       1170       1180       1190       1200 
LQRLSMASSG SLWQEIPVVR NSTVLLSMTH EDQKLQEVKF ELIVSEASYL RSLNIAVDHF 

      1210       1220       1230       1240       1250       1260 
QLSTSLRATL SNQEHQWLFS RLQDVRDVSA TFLSDLEENF ENNIFSFQVC DVVLNHAPDF 

      1270       1280       1290       1300       1310       1320 
RRVYLPYVTN QTYQERTFQS LMNSNSNFRE VLEKLESDPV CQRLSLKSFL ILPFQRITRL 

      1330       1340       1350       1360       1370       1380 
KLLLQNILKR TQPGSSEEAE ATKAHHALEQ LIRDCNNNVQ SMRRTEELIY LSQKIEFECK 

      1390       1400       1410       1420       1430       1440 
IFPLISQSRW LVKSGELTAL EFSASPGLRR KLNTRPVHLH LFNDCLLLSR PREGSRFLVF 

      1450       1460       1470       1480       1490       1500 
DHAPFSSIRG EKCEMKLHGP HKNLFRLFLR QNTQGAQAEF LFRTETQSEK LRWISALAMP 

      1510       1520       1530       1540       1550       1560 
REELDLLECY NSPQVQCLRA YKPRENDELA LEKADVVMVT QQSSDGWLEG VRLSDGERGW 

      1570       1580       1590 
FPVQQVEFIS NPEVRAQNLK EAHRVKTAKL QLVEQQA 

« Hide

Isoform 2 [UniParc].

Checksum: 2EE2C16B3F42C053
Show »

FASTA51960,056

References

« Hide 'large scale' references
[1]"Expression cDNA cloning of a novel oncogene with sequence similarity to regulators of small GTP-binding proteins."
Chan A.M.-L., McGovern E.S., Catalano G., Fleming T.P., Miki T.
Oncogene 9:1057-1063(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Epithelium.
[2]"The nucleotide sequence of a long cDNA clone isolated from human spleen."
Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O.
Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Spleen.
[3]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Placenta and Skin.
[6]"Eph-dependent tyrosine phosphorylation of ephexin1 modulates growth cone collapse."
Sahin M., Greer P.L., Lin M.Z., Poucher H., Eberhart J., Schmidt S., Wright T.M., Shamah S.M., O'connell S., Cowan C.W., Hu L., Goldberg J.L., Debant A., Corfas G., Krull C.E., Greenberg M.E.
Neuron 46:191-204(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
[7]"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-445 AND SER-450, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-184; SER-445; SER-450 AND SER-1126, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-445; SER-450 AND SER-1126, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U02082 mRNA. Translation: AAA18010.1.
AK160365 mRNA. Translation: BAD18708.1. Different initiation.
AC004534 Genomic DNA. Translation: AAC12958.1.
CH471146 Genomic DNA. Translation: EAW80096.1.
BC010046 mRNA. Translation: AAH10046.1.
BC011839 mRNA. Translation: AAH11839.1.
BC014555 mRNA. Translation: AAH14555.1.
PIRI38402.
RefSeqNP_005426.2. NM_005435.3.
UniGeneHs.334.
Hs.534621.

3D structure databases

ProteinModelPortalQ12774.
SMRQ12774. Positions 1168-1506, 1513-1572.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113696. 14 interactions.
IntActQ12774. 13 interactions.
MINTMINT-3974855.
STRING9606.ENSP00000056217.

PTM databases

PhosphoSiteQ12774.

Polymorphism databases

DMDM290457679.

Proteomic databases

PaxDbQ12774.
PRIDEQ12774.

Protocols and materials databases

DNASU7984.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000056217; ENSP00000056217; ENSG00000050327. [Q12774-1]
ENST00000471847; ENSP00000418227; ENSG00000050327. [Q12774-2]
GeneID7984.
KEGGhsa:7984.
UCSCuc003wek.3. human. [Q12774-1]

Organism-specific databases

CTD7984.
GeneCardsGC07P144052.
HGNCHGNC:13209. ARHGEF5.
HPAHPA020980.
HPA029504.
MIM600888. gene.
neXtProtNX_Q12774.
PharmGKBPA24975.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5422.
HOGENOMHOG000170375.
HOVERGENHBG060842.
InParanoidQ12774.
OMAQARQPEK.
OrthoDBEOG7NPFSZ.
PhylomeDBQ12774.
TreeFamTF342609.

Gene expression databases

ArrayExpressQ12774.
BgeeQ12774.
CleanExHS_ARHGEF5.
GenevestigatorQ12774.

Family and domain databases

Gene3D1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProIPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR001452. SH3_domain.
[Graphical view]
PfamPF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF48065. SSF48065. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEPS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiARHGEF5.
GenomeRNAi7984.
NextBio30518.
PROQ12774.
SOURCESearch...

Entry information

Entry nameARHG5_HUMAN
AccessionPrimary (citable) accession number: Q12774
Secondary accession number(s): A6NNJ2, Q6ZML7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: March 2, 2010
Last modified: April 16, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM