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Q12774

- ARHG5_HUMAN

UniProt

Q12774 - ARHG5_HUMAN

Protein

Rho guanine nucleotide exchange factor 5

Gene

ARHGEF5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 3 (02 Mar 2010)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. GTP binding Source: ProtInc
    2. protein binding Source: IntAct
    3. Rho guanyl-nucleotide exchange factor activity Source: InterPro

    GO - Biological processi

    1. actin cytoskeleton organization Source: Ensembl
    2. intracellular signal transduction Source: InterPro
    3. myeloid dendritic cell chemotaxis Source: Ensembl
    4. positive regulation of podosome assembly Source: Ensembl
    5. regulation of Rho GTPase activity Source: MGI

    Keywords - Molecular functioni

    Guanine-nucleotide releasing factor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rho guanine nucleotide exchange factor 5
    Alternative name(s):
    Ephexin-3
    Guanine nucleotide regulatory protein TIM
    Oncogene TIM
    Transforming immortalized mammary oncogene
    p60 TIM
    Gene namesi
    Name:ARHGEF5
    Synonyms:TIM
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:13209. ARHGEF5.

    Pathology & Biotechi

    Keywords - Diseasei

    Proto-oncogene

    Organism-specific databases

    PharmGKBiPA24975.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15971597Rho guanine nucleotide exchange factor 5PRO_0000080916Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei184 – 1841Phosphoserine1 Publication
    Modified residuei445 – 4451Phosphoserine3 Publications
    Modified residuei450 – 4501Phosphoserine3 Publications
    Modified residuei1126 – 11261Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ12774.
    PaxDbiQ12774.
    PRIDEiQ12774.

    PTM databases

    PhosphoSiteiQ12774.

    Expressioni

    Tissue specificityi

    Mainly expressed in kidney, liver, pancreas, lung and placenta.

    Gene expression databases

    ArrayExpressiQ12774.
    BgeeiQ12774.
    CleanExiHS_ARHGEF5.
    GenevestigatoriQ12774.

    Organism-specific databases

    HPAiHPA020980.
    HPA029504.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GRB2P629934EBI-602199,EBI-401755

    Protein-protein interaction databases

    BioGridi113696. 14 interactions.
    IntActiQ12774. 13 interactions.
    MINTiMINT-3974855.
    STRINGi9606.ENSP00000056217.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12774.
    SMRiQ12774. Positions 1168-1506, 1513-1572.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1174 – 1358185DHPROSITE-ProRule annotationAdd
    BLAST
    Domaini1390 – 1502113PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini1513 – 156957SH3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain

    Phylogenomic databases

    eggNOGiCOG5422.
    HOGENOMiHOG000170375.
    HOVERGENiHBG060842.
    InParanoidiQ12774.
    OMAiQARQPEK.
    OrthoDBiEOG7NPFSZ.
    PhylomeDBiQ12774.
    TreeFamiTF342609.

    Family and domain databases

    Gene3Di1.20.900.10. 1 hit.
    2.30.29.30. 1 hit.
    InterProiIPR029212. ARHGEF5/35_N.
    IPR000219. DH-domain.
    IPR001331. GDS_CDC24_CS.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF15441. ARHGEF5_35. 1 hit.
    PF00621. RhoGEF. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view]
    SMARTiSM00233. PH. 1 hit.
    SM00325. RhoGEF. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF48065. SSF48065. 1 hit.
    SSF50044. SSF50044. 1 hit.
    PROSITEiPS00741. DH_1. 1 hit.
    PS50010. DH_2. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q12774-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEAEEAQRGA SPPISAIEEF SIIPEAPMRS SQVSALGLEA QEDEDPSYKW     50
    REEHRLSATQ QSELRDVCDY AIETMPSFPK EGSADVEPNQ ESLVAEACDT 100
    PEHWEAVPQS LAGRQARTLA PPELWACPIQ SEHLDMAPFS SDLGSEEEEV 150
    EFWPGLTSLT LGSGQAEEEE ETSSDNSGQT RYYSPCEEHP AETNQNEGSE 200
    SGTIRQGEEL PPEELQESQG LLHPQEVQVL EEQGQQEAGF RGEGTLREDV 250
    CADGLLGEEQ MIEQVNDEKG EQKQKQEQVQ DVMLGRQGER MGLTGEPEGL 300
    NDGEWEQEDM ERKAQGQGGP EQGEERKREL QVPEENRADS QDEKSQTFLG 350
    KSEEVTGKQE DHGIKEKGVP VSGQEAKEPE SWDGGRLGAV GRARSREEEN 400
    EHHGPSMPAL IAPEDSPHCD LFPGASYLMT QIPGTQTESR AEELSPAALS 450
    PSLEPIRCSH QPISLLGSFL TEESPDKEID QNSQQEESRL RKGTVSSQGT 500
    EVVFASASVT PPRTPDSAPP SPAEAYPITP ASVSARPPVA FPRRETSCAA 550
    RAPETASAPL SMDDPSPCGT SEMCPAALYG FPSTGTSPPR PPANSTGTVQ 600
    HLRSDSFPGS HRTEQTPDLV GMLLSYSHSE LPQRPPKPAI YSSVTPRRDR 650
    RSGRDYSTVS ASPTALSTLK QDSQESISNL ERPSSPPSIQ PWVSPHNPAF 700
    ATESPAYGSS PSFVSMEDVR IHEPLPPPPP QRRDTHPSVV ETDGHARVVV 750
    PTLKQHSHPP PLALGSGLHA PHKGPLPQAS DPAVARQHRP LPSTPDSSHH 800
    AQATPRWRYN KPLPPTPDLP QPHLPPISAP GSSRIYRPLP PLPIIDPPTE 850
    PPPLPPKSRG RSRSTRGGHM NSGGHAKTRP ACQDWTVPLP ASAGRTSWPP 900
    ATARSTESFT STSRSKSEVS PGMAFSNMTN FLCPSSPTTP WTPELQGPTS 950
    KDEAGVSEHP EAPAREPLRR TTPQQGASGP GRSPVGQARQ PEKPSHLHLE 1000
    KASSWPHRRD SGRPPGDSSG QAVAPSEGAN KHKGWSRQGL RRPSILPEGS 1050
    SDSRGPAVEK HPGPSDTVVF REKKPKEVMG GFSRRCSKLI NSSQLLYQEY 1100
    SDVVLNKEIQ SQQRLESLSE TPGPSSPRQP RKALVSSESY LQRLSMASSG 1150
    SLWQEIPVVR NSTVLLSMTH EDQKLQEVKF ELIVSEASYL RSLNIAVDHF 1200
    QLSTSLRATL SNQEHQWLFS RLQDVRDVSA TFLSDLEENF ENNIFSFQVC 1250
    DVVLNHAPDF RRVYLPYVTN QTYQERTFQS LMNSNSNFRE VLEKLESDPV 1300
    CQRLSLKSFL ILPFQRITRL KLLLQNILKR TQPGSSEEAE ATKAHHALEQ 1350
    LIRDCNNNVQ SMRRTEELIY LSQKIEFECK IFPLISQSRW LVKSGELTAL 1400
    EFSASPGLRR KLNTRPVHLH LFNDCLLLSR PREGSRFLVF DHAPFSSIRG 1450
    EKCEMKLHGP HKNLFRLFLR QNTQGAQAEF LFRTETQSEK LRWISALAMP 1500
    REELDLLECY NSPQVQCLRA YKPRENDELA LEKADVVMVT QQSSDGWLEG 1550
    VRLSDGERGW FPVQQVEFIS NPEVRAQNLK EAHRVKTAKL QLVEQQA 1597
    Length:1,597
    Mass (Da):176,799
    Last modified:March 2, 2010 - v3
    Checksum:i93EC1B1D7E1198C5
    GO
    Isoform 2 (identifier: Q12774-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1078: Missing.

    Show »
    Length:519
    Mass (Da):60,056
    Checksum:i2EE2C16B3F42C053
    GO

    Sequence cautioni

    The sequence BAD18708.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti487 – 4871E → G in BAD18708. 1 PublicationCurated
    Sequence conflicti575 – 5751P → Q in BAD18708. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 10781078Missing in isoform 2. 2 PublicationsVSP_035175Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U02082 mRNA. Translation: AAA18010.1.
    AK160365 mRNA. Translation: BAD18708.1. Different initiation.
    AC004534 Genomic DNA. Translation: AAC12958.1.
    CH471146 Genomic DNA. Translation: EAW80096.1.
    BC010046 mRNA. Translation: AAH10046.1.
    BC011839 mRNA. Translation: AAH11839.1.
    BC014555 mRNA. Translation: AAH14555.1.
    CCDSiCCDS34771.1. [Q12774-1]
    PIRiI38402.
    RefSeqiNP_005426.2. NM_005435.3. [Q12774-1]
    XP_006716190.1. XM_006716127.1. [Q12774-1]
    UniGeneiHs.334.
    Hs.534621.

    Genome annotation databases

    EnsembliENST00000056217; ENSP00000056217; ENSG00000050327. [Q12774-1]
    ENST00000471847; ENSP00000418227; ENSG00000050327. [Q12774-2]
    GeneIDi7984.
    KEGGihsa:7984.
    UCSCiuc003wek.3. human. [Q12774-1]

    Polymorphism databases

    DMDMi290457679.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U02082 mRNA. Translation: AAA18010.1 .
    AK160365 mRNA. Translation: BAD18708.1 . Different initiation.
    AC004534 Genomic DNA. Translation: AAC12958.1 .
    CH471146 Genomic DNA. Translation: EAW80096.1 .
    BC010046 mRNA. Translation: AAH10046.1 .
    BC011839 mRNA. Translation: AAH11839.1 .
    BC014555 mRNA. Translation: AAH14555.1 .
    CCDSi CCDS34771.1. [Q12774-1 ]
    PIRi I38402.
    RefSeqi NP_005426.2. NM_005435.3. [Q12774-1 ]
    XP_006716190.1. XM_006716127.1. [Q12774-1 ]
    UniGenei Hs.334.
    Hs.534621.

    3D structure databases

    ProteinModelPortali Q12774.
    SMRi Q12774. Positions 1168-1506, 1513-1572.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113696. 14 interactions.
    IntActi Q12774. 13 interactions.
    MINTi MINT-3974855.
    STRINGi 9606.ENSP00000056217.

    PTM databases

    PhosphoSitei Q12774.

    Polymorphism databases

    DMDMi 290457679.

    Proteomic databases

    MaxQBi Q12774.
    PaxDbi Q12774.
    PRIDEi Q12774.

    Protocols and materials databases

    DNASUi 7984.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000056217 ; ENSP00000056217 ; ENSG00000050327 . [Q12774-1 ]
    ENST00000471847 ; ENSP00000418227 ; ENSG00000050327 . [Q12774-2 ]
    GeneIDi 7984.
    KEGGi hsa:7984.
    UCSCi uc003wek.3. human. [Q12774-1 ]

    Organism-specific databases

    CTDi 7984.
    GeneCardsi GC07P144052.
    HGNCi HGNC:13209. ARHGEF5.
    HPAi HPA020980.
    HPA029504.
    MIMi 600888. gene.
    neXtProti NX_Q12774.
    PharmGKBi PA24975.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5422.
    HOGENOMi HOG000170375.
    HOVERGENi HBG060842.
    InParanoidi Q12774.
    OMAi QARQPEK.
    OrthoDBi EOG7NPFSZ.
    PhylomeDBi Q12774.
    TreeFami TF342609.

    Miscellaneous databases

    GeneWikii ARHGEF5.
    GenomeRNAii 7984.
    NextBioi 30518.
    PROi Q12774.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q12774.
    Bgeei Q12774.
    CleanExi HS_ARHGEF5.
    Genevestigatori Q12774.

    Family and domain databases

    Gene3Di 1.20.900.10. 1 hit.
    2.30.29.30. 1 hit.
    InterProi IPR029212. ARHGEF5/35_N.
    IPR000219. DH-domain.
    IPR001331. GDS_CDC24_CS.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF15441. ARHGEF5_35. 1 hit.
    PF00621. RhoGEF. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view ]
    SMARTi SM00233. PH. 1 hit.
    SM00325. RhoGEF. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48065. SSF48065. 1 hit.
    SSF50044. SSF50044. 1 hit.
    PROSITEi PS00741. DH_1. 1 hit.
    PS50010. DH_2. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression cDNA cloning of a novel oncogene with sequence similarity to regulators of small GTP-binding proteins."
      Chan A.M.-L., McGovern E.S., Catalano G., Fleming T.P., Miki T.
      Oncogene 9:1057-1063(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Epithelium.
    2. "The nucleotide sequence of a long cDNA clone isolated from human spleen."
      Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O.
      Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Spleen.
    3. "The DNA sequence of human chromosome 7."
      Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
      , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
      Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Placenta and Skin.
    6. Cited for: NOMENCLATURE.
    7. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
      Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
      Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-445 AND SER-450, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-184; SER-445; SER-450 AND SER-1126, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-445; SER-450 AND SER-1126, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiARHG5_HUMAN
    AccessioniPrimary (citable) accession number: Q12774
    Secondary accession number(s): A6NNJ2, Q6ZML7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: March 2, 2010
    Last modified: October 1, 2014
    This is version 132 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3