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Q12754 (RRP12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal RNA-processing protein 12
Gene names
Name:RRP12
Ordered Locus Names:YPL012W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1228 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

In association with GSP1, required for nuclear export of both pre-40S and pre-60S ribosomal subunits. Required for the late maturation of the 18S and 5.8S rRNA of the pre-40S ribosomes and for maturation of the 25S and 5.8S rRNA of the pre-60S ribosomes. Ref.6

Subunit structure

Interacts with GSP1. Ref.6

Subcellular location

Cytoplasm. Nucleusnucleolus Ref.3 Ref.4 Ref.6.

Miscellaneous

Present with 8170 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the RRP12 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

EBP2P360491EBI-30678,EBI-6289

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12281228Ribosomal RNA-processing protein 12
PRO_0000270563

Amino acid modifications

Modified residue10591Phosphoserine Ref.9
Modified residue10671Phosphoserine Ref.7 Ref.8 Ref.9

Sequences

Sequence LengthMass (Da)Tools
Q12754 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 92333BCD53CB095F

FASTA1,228137,509
        10         20         30         40         50         60 
MDQDKVAFLL ELEDKLAKIR SQVNSKLENQ KHIAIILTAV EENIAGQATN DVSKNIVNYI 

        70         80         90        100        110        120 
ISFMSLLDQA VDPSTHEIKD IQLASSSTYL LDLIFHYSPK VLLRSKFSEI LTKIAPCITA 

       130        140        150        160        170        180 
EKANAPLIRA AIGCLESLLI AQDAQAWNNT YDLNVTPKRG LQGILELSLD VRPKVRKRAL 

       190        200        210        220        230        240 
DAVHAVLLNP PVAPTAEHVA AVFVADFCDK QLAGILNDLS NLSNKQLKAQ KTKEDINASV 

       250        260        270        280        290        300 
MRSLRLITSV VSTGQWPSSQ IEPLCDVLLG VTKSSEQYLV SASFECFESM FKTMAETTIS 

       310        320        330        340        350        360 
SGLAENKYLR VLDTIFALKP SNVDTLLTKS WIAVVIKGMS TYATHQPLKA LRKIPGVFHI 

       370        380        390        400        410        420 
MCTYLASETP EVYQAASQCL ISILSESVKD DLLLYTPSVD EKVFKNVDEI ISQIAKTFID 

       430        440        450        460        470        480 
FLSIRYSHCS REILKILVAA FNKFRYRSNP HFLKSLKIVD TWRVNEEQFM DLRNEIELVI 

       490        500        510        520        530        540 
GASISAMGPE MILAEAPLNL DNPSSERPGR AWLLPLIRDY TKNANLATFQ NELAPYIKSF 

       550        560        570        580        590        600 
QSKFDKVPEE SIQLRVFQTI VDQIWSTLPR FCELPMDLRE SFTDEFASEL SSLLYSEVEL 

       610        620        630        640        650        660 
RTTICHALKV LAESNVSYAE ESSSHNVLLL QRFPISEAQK NIEYLSTKST NLLAVLFNVY 

       670        680        690        700        710        720 
TQTTPNARSY ILETIDQYLK ITSKEDLEKT FNNVCGLLKN SMNEESSGNV NKEKKKPQLT 

       730        740        750        760        770        780 
ATLLDLIICM ITYLPVSSYS ALFSMFSLTV NSADALIQKR AYRIITKLSE LKSGSTAVAQ 

       790        800        810        820        830        840 
FISDIENVMV DSASSVQTSA KAARLTAIKT IVELLPLDHL DFIVRTVAEV ILSTKDVNEK 

       850        860        870        880        890        900 
SRETAFDTLI CMGRKMNEPN GIIKLFQIPG YDPTTPDQSS SISEFFKIIS AGLIGESQHM 

       910        920        930        940        950        960 
VSSSITGYAC LVFEFKNELD SGILMDIYDT IELYLTSNSR EIVKSAIGFT KVCVLGLPEE 

       970        980        990       1000       1010       1020 
LMRPKVPELL LKLLRWSHEH TGHFKAKVKH IIERLIRRFG YDYIEANFPE EDRRLLTNIR 

      1030       1040       1050       1060       1070       1080 
KMRNRNKRKD EEVTTGVSDV AATKGSRFMS AFDEAVYGSD EENDNGSDQE ENVAGGKMKN 

      1090       1100       1110       1120       1130       1140 
GAKQFIVESG DNPLDLLDSQ TLAHISSTRP KKFNKNQNRA RFNDDAFNFD SEGKLVVKGQ 

      1150       1160       1170       1180       1190       1200 
PKPSTNVDDP LSAVTSGINA YLEAVKSGPV RGQRNKLKFR KNGKDSDEFG DDDDGEKDSR 

      1210       1220 
LMRGRVNQGN KIGKHNKKGP KFKSRKKL 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes."
Schaefer T., Strauss D., Petfalski E., Tollervey D., Hurt E.
EMBO J. 22:1370-1380(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A pre-ribosome-associated HEAT-repeat protein is required for export of both ribosomal subunits."
Oeffinger M., Dlakic M., Tollervey D.
Genes Dev. 18:196-209(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH GSP1, SUBCELLULAR LOCATION.
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1067, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1067, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1059 AND SER-1067, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U33335 Genomic DNA. Translation: AAB68093.1.
Z71255 Genomic DNA. Translation: CAA95029.1.
Z48483 Genomic DNA. Translation: CAA88374.1.
BK006949 Genomic DNA. Translation: DAA11416.1.
PIRS59681.
RefSeqNP_015313.1. NM_001183826.1.

3D structure databases

ProteinModelPortalQ12754.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36165. 115 interactions.
DIPDIP-6497N.
IntActQ12754. 49 interactions.
MINTMINT-639557.
STRING4932.YPL012W.

Proteomic databases

PaxDbQ12754.
PeptideAtlasQ12754.
PRIDEQ12754.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL012W; YPL012W; YPL012W.
GeneID856095.
KEGGsce:YPL012W.

Organism-specific databases

CYGDYPL012w.
SGDS000005933. RRP12.

Phylogenomic databases

eggNOGNOG149372.
GeneTreeENSGT00390000013106.
HOGENOMHOG000164239.
KOK14794.
OMANTNLAHF.
OrthoDBEOG7WDN9V.

Enzyme and pathway databases

BioCycYEAST:G3O-33931-MONOMER.

Gene expression databases

GenevestigatorQ12754.

Family and domain databases

InterProIPR016024. ARM-type_fold.
IPR012978. Uncharacterised_NUC173.
[Graphical view]
PfamPF08161. NUC173. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 2 hits.
ProtoNetSearch...

Other

NextBio981131.
PROQ12754.

Entry information

Entry nameRRP12_YEAST
AccessionPrimary (citable) accession number: Q12754
Secondary accession number(s): D6W400, Q7LH12
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families