Q12754 (RRP12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA-processing protein 12

Gene names

Name:	RRP12
Ordered Locus Names:	YPL012W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1228 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	In association with GSP1, required for nuclear export of both pre-40S and pre-60S ribosomal subunits. Required for the late maturation of the 18S and 5.8S rRNA of the pre-40S ribosomes and for maturation of the 25S and 5.8S rRNA of the pre-60S ribosomes. Ref.6
Subunit structure	Interacts with GSP1. Ref.6
Subcellular location	Cytoplasm. Nucleus › nucleolus Ref.3 Ref.4 Ref.6.
Miscellaneous	Present with 8170 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the RRP12 family.

Ontologies

Keywords
Biological process	Ribosome biogenesis
Cellular component	Cytoplasm Nucleus
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Traceable author statement PubMed 12067653. Source: SGD
Cellular_component	90S preribosome Inferred from direct assay PubMed 12150911. Source: SGD nucleolus Inferred from direct assay PubMed 22842922. Source: SGD ribosome Traceable author statement PubMed 12067653. Source: SGD
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EBP2	P36049	1	EBI-30678,EBI-6289

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1228	1228	Ribosomal RNA-processing protein 12		PRO_0000270563
Amino acid modifications
	Modified residue	1067	1	Phosphoserine Ref.7 Ref.9
	Modified residue	1186	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q12754 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 92333BCD53CB095F	FASTA	1,228	137,509
	10 20 30 40 50 60 MDQDKVAFLL ELEDKLAKIR SQVNSKLENQ KHIAIILTAV EENIAGQATN DVSKNIVNYI 70 80 90 100 110 120 ISFMSLLDQA VDPSTHEIKD IQLASSSTYL LDLIFHYSPK VLLRSKFSEI LTKIAPCITA 130 140 150 160 170 180 EKANAPLIRA AIGCLESLLI AQDAQAWNNT YDLNVTPKRG LQGILELSLD VRPKVRKRAL 190 200 210 220 230 240 DAVHAVLLNP PVAPTAEHVA AVFVADFCDK QLAGILNDLS NLSNKQLKAQ KTKEDINASV 250 260 270 280 290 300 MRSLRLITSV VSTGQWPSSQ IEPLCDVLLG VTKSSEQYLV SASFECFESM FKTMAETTIS 310 320 330 340 350 360 SGLAENKYLR VLDTIFALKP SNVDTLLTKS WIAVVIKGMS TYATHQPLKA LRKIPGVFHI 370 380 390 400 410 420 MCTYLASETP EVYQAASQCL ISILSESVKD DLLLYTPSVD EKVFKNVDEI ISQIAKTFID 430 440 450 460 470 480 FLSIRYSHCS REILKILVAA FNKFRYRSNP HFLKSLKIVD TWRVNEEQFM DLRNEIELVI 490 500 510 520 530 540 GASISAMGPE MILAEAPLNL DNPSSERPGR AWLLPLIRDY TKNANLATFQ NELAPYIKSF 550 560 570 580 590 600 QSKFDKVPEE SIQLRVFQTI VDQIWSTLPR FCELPMDLRE SFTDEFASEL SSLLYSEVEL 610 620 630 640 650 660 RTTICHALKV LAESNVSYAE ESSSHNVLLL QRFPISEAQK NIEYLSTKST NLLAVLFNVY 670 680 690 700 710 720 TQTTPNARSY ILETIDQYLK ITSKEDLEKT FNNVCGLLKN SMNEESSGNV NKEKKKPQLT 730 740 750 760 770 780 ATLLDLIICM ITYLPVSSYS ALFSMFSLTV NSADALIQKR AYRIITKLSE LKSGSTAVAQ 790 800 810 820 830 840 FISDIENVMV DSASSVQTSA KAARLTAIKT IVELLPLDHL DFIVRTVAEV ILSTKDVNEK 850 860 870 880 890 900 SRETAFDTLI CMGRKMNEPN GIIKLFQIPG YDPTTPDQSS SISEFFKIIS AGLIGESQHM 910 920 930 940 950 960 VSSSITGYAC LVFEFKNELD SGILMDIYDT IELYLTSNSR EIVKSAIGFT KVCVLGLPEE 970 980 990 1000 1010 1020 LMRPKVPELL LKLLRWSHEH TGHFKAKVKH IIERLIRRFG YDYIEANFPE EDRRLLTNIR 1030 1040 1050 1060 1070 1080 KMRNRNKRKD EEVTTGVSDV AATKGSRFMS AFDEAVYGSD EENDNGSDQE ENVAGGKMKN 1090 1100 1110 1120 1130 1140 GAKQFIVESG DNPLDLLDSQ TLAHISSTRP KKFNKNQNRA RFNDDAFNFD SEGKLVVKGQ 1150 1160 1170 1180 1190 1200 PKPSTNVDDP LSAVTSGINA YLEAVKSGPV RGQRNKLKFR KNGKDSDEFG DDDDGEKDSR 1210 1220 LMRGRVNQGN KIGKHNKKGP KFKSRKKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes." Schaefer T., Strauss D., Petfalski E., Tollervey D., Hurt E. EMBO J. 22:1370-1380(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A pre-ribosome-associated HEAT-repeat protein is required for export of both ribosomal subunits." Oeffinger M., Dlakic M., Tollervey D. Genes Dev. 18:196-209(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH GSP1, SUBCELLULAR LOCATION.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1067, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1186, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1067, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U33335 Genomic DNA. Translation: AAB68093.1. Z71255 Genomic DNA. Translation: CAA95029.1. Z48483 Genomic DNA. Translation: CAA88374.1. BK006949 Genomic DNA. Translation: DAA11416.1.
PIR	S59681.
RefSeq	NP_015313.1. NM_001183826.1.
3D structure databases
ProteinModelPortal	Q12754.
SMR	Q12754. Positions 86-188, 355-385, 821-853, 927-958.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6497N.
IntAct	Q12754. 52 interactions.
MINT	MINT-639557.
STRING	4932.YPL012W.
Proteomic databases
PaxDb	Q12754.
PeptideAtlas	Q12754.
PRIDE	Q12754.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL012W; YPL012W; YPL012W.
GeneID	856095.
KEGG	sce:YPL012W.
Organism-specific databases
CYGD	YPL012w.
SGD	S000005933. RRP12.
Phylogenomic databases
eggNOG	NOG149372.
GeneTree	ENSGT00390000013106.
HOGENOM	HOG000164239.
KO	K14794.
OMA	LLCVFFE.
OrthoDB	EOG4WQ49N.
Enzyme and pathway databases
BioCyc	YEAST:G3O-33931-MONOMER.
Gene expression databases
Genevestigator	Q12754.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR012978. Uncharacterised_NUC173. [Graphical view]
Pfam	PF08161. NUC173. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
ProtoNet	Search...
Other
NextBio	981131.

Entry information

Entry name

RRP12_YEAST

Accession

Primary (citable) accession number: Q12754
Secondary accession number(s): D6W400, Q7LH12

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program