Q12753 (HAA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional activator HAA1

Gene names

Name:	HAA1
Ordered Locus Names:	YPR008W
ORF Names:	LPZ8W, YP9531.01, YP9723.08

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	694 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulates the transcription of a set of genes, many of which encode membrane proteins. Among the genes regulated are YGR138C and YRO2. Does not seem to be dependent on copper. Ref.4
Subcellular location	Nucleus Ref.4.
Miscellaneous	Present with 339 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 copper-fist DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	Copper DNA-binding Metal-binding Zinc
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription from RNA polymerase II promoter in response to stress Inferred from mutant phenotype PubMed 16176797PubMed 16980434. Source: SGD response to acid Inferred from mutant phenotype PubMed 16176797PubMed 16980434. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 22842922. Source: SGD nucleus Inferred from direct assay Ref.4. Source: SGD
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW copper ion binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding RNA polymerase II transcription factor activity Inferred from direct assay PubMed 21586585. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 694	694	Transcriptional activator HAA1		PRO_0000194933
Regions
	DNA binding	1 – 40	40	Copper-fist
Sites
	Metal binding	11	1	Zinc By similarity
	Metal binding	14	1	Zinc By similarity
	Metal binding	23	1	Zinc By similarity
	Metal binding	25	1	Zinc By similarity
Amino acid modifications
	Modified residue	125	1	Phosphoserine Ref.8
	Modified residue	134	1	Phosphoserine Ref.8
	Modified residue	137	1	Phosphoserine Ref.8
	Modified residue	241	1	Phosphoserine Ref.8
	Modified residue	248	1	Phosphoserine Ref.8
	Modified residue	271	1	Phosphoserine Ref.8
	Modified residue	437	1	Phosphoserine Ref.6
	Modified residue	439	1	Phosphoserine Ref.6
	Modified residue	506	1	Phosphoserine Ref.7 Ref.8
	Modified residue	566	1	Phosphoserine Ref.8
	Modified residue	570	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q12753 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0A129F79B4E99CA8	FASTA	694	76,671
	10 20 30 40 50 60 MVLINGIKYA CERCIRGHRV TTCNHTDQPL MMIKPKGRPS TTCDYCKQLR KNKNANPEGV 70 80 90 100 110 120 CTCGRLEKKK LAQKAKEEAR AKAKEKQRKQ CTCGTDEVCK YHAQKRHLRK SPSSSQKKGR 130 140 150 160 170 180 SISRSQPMFE RVLSSTSLDS NMLSGHGALS DTSSILTSTF LDSEPGVGKI SKDYHHVPSL 190 200 210 220 230 240 ASISSLQSSQ SLDQNFSIPQ SPPLSSMSFN FLTGNINETN QNHSNHQHSK SGNNWQDSSV 250 260 270 280 290 300 SLPAKADSRL NMMDKNNSVG LDLLGHSKRI SPISNSRVGE VSVPLEEYIP SDIDGVGRVT 310 320 330 340 350 360 DKSSLVYDWP FDESIERNFS TTATAATGES KFDINDNCNR INSKSYSKTN SMNGNGMNNS 370 380 390 400 410 420 NNNNINSNGN DKNNNNSSRQ EHQGNGLFDM FTDSSSISTL SRANLLLQEK IGSQENSVKQ 430 440 450 460 470 480 ENYSKNPQLR HQLTSRSRSF IHHPANEYLK NTFGNSHSND IGKGVEVLSL TPSFMDIPEK 490 500 510 520 530 540 ERETERSPSS NYITDRPFTR KPRSSSIDVN HRYPPMAPTT VATSPGALNN AVASNLDDQL 550 560 570 580 590 600 SLTSLNSQPS SIANMMMDPS NLAEQSSIHS VPQSINSPRM PKTGSRQDKN IHTKKEERNP 610 620 630 640 650 660 LNNIHDLSQL ENVPDEMNQM FSPPLKSMNR PDAIRENSSS SNFIIQGNSM ISTPSGRNDL 670 680 690 PDTSPMSSIQ TASPPSQLLT DQGFADLDNF MSSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Haa1, a protein homologous to the copper-regulated transcription factor ace1, is a novel transcriptional activator." Keller G., Ray E., Brown P.O., Winge D.R. J. Biol. Chem. 276:38697-38702(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-437 AND SER-439, MASS SPECTROMETRY.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-506, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-125; SER-134; SER-137; SER-241; SER-248; SER-271; SER-506; SER-566 AND SER-570, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U31900 Genomic DNA. Translation: AAA97587.1. Z71255 Genomic DNA. Translation: CAA95048.1. Z48951 Genomic DNA. Translation: CAA88786.1. Z49919 Genomic DNA. Translation: CAA90152.1. AY692804 Genomic DNA. Translation: AAT92823.1. BK006949 Genomic DNA. Translation: DAA11435.1.
PIR	S59753.
RefSeq	NP_015333.1. NM_001184105.1.
3D structure databases
ProteinModelPortal	Q12753.
SMR	Q12753. Positions 1-42.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1462N.
IntAct	Q12753. 6 interactions.
MINT	MINT-402478.
STRING	4932.YPR008W.
Proteomic databases
PaxDb	Q12753.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPR008W; YPR008W; YPR008W.
GeneID	856117.
KEGG	sce:YPR008W.
Organism-specific databases
CYGD	YPR008w.
SGD	S000006212. HAA1.
Phylogenomic databases
eggNOG	NOG71500.
GeneTree	ENSGT00530000068810.
OMA	CTCGRLE.
OrthoDB	EOG4X0R35.
Gene expression databases
Genevestigator	Q12753.
GermOnline	YPR008W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.90.430.10. 1 hit.
InterPro	IPR001083. Cu_fist_DNA-bd. [Graphical view]
Pfam	PF00649. Copper-fist. 1 hit. [Graphical view]
PRINTS	PR00617. COPPERFIST.
SMART	SM00412. Cu_FIST. 1 hit. [Graphical view]
SUPFAM	SSF57879. Cu_fist_DNA_bd. 1 hit.
PROSITE	PS01119. COPPER_FIST_1. 1 hit. PS50073. COPPER_FIST_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981192.

Entry information

Entry name

HAA1_YEAST

Accession

Primary (citable) accession number: Q12753
Secondary accession number(s): D6W419

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program