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Q12753

- HAA1_YEAST

UniProt

Q12753 - HAA1_YEAST

Protein

Transcriptional activator HAA1

Gene

HAA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Regulates the transcription of a set of genes, many of which encode membrane proteins. Among the genes regulated are YGR138C and YRO2. Does not seem to be dependent on copper.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi11 – 111ZincPROSITE-ProRule annotation
    Metal bindingi14 – 141ZincPROSITE-ProRule annotation
    Metal bindingi23 – 231ZincPROSITE-ProRule annotation
    Metal bindingi25 – 251ZincPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi1 – 4040Copper-fistPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. copper ion binding Source: InterPro
    2. DNA binding Source: UniProtKB-KW
    3. protein binding Source: IntAct
    4. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: SGD

    GO - Biological processi

    1. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    2. positive regulation of transcription from RNA polymerase II promoter in response to stress Source: SGD
    3. response to acid chemical Source: SGD
    4. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Copper, DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34170-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional activator HAA1
    Gene namesi
    Name:HAA1
    Ordered Locus Names:YPR008W
    ORF Names:LPZ8W, YP9531.01, YP9723.08
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR008w.
    SGDiS000006212. HAA1.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 694694Transcriptional activator HAA1PRO_0000194933Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei125 – 1251Phosphoserine1 Publication
    Modified residuei231 – 2311Phosphoserine1 Publication
    Modified residuei241 – 2411Phosphoserine2 Publications
    Modified residuei291 – 2911Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ12753.
    PaxDbiQ12753.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12753.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    FUS1P117103EBI-8118,EBI-7179

    Protein-protein interaction databases

    BioGridi36186. 37 interactions.
    DIPiDIP-1462N.
    IntActiQ12753. 7 interactions.
    MINTiMINT-402478.
    STRINGi4932.YPR008W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12753.
    SMRiQ12753. Positions 1-42.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 copper-fist DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG71500.
    GeneTreeiENSGT00530000068810.
    OMAiCTCGRLE.
    OrthoDBiEOG7J9W29.

    Family and domain databases

    Gene3Di3.90.430.10. 1 hit.
    InterProiIPR001083. Cu_fist_DNA-bd.
    [Graphical view]
    PfamiPF00649. Copper-fist. 1 hit.
    [Graphical view]
    PRINTSiPR00617. COPPERFIST.
    SMARTiSM00412. Cu_FIST. 1 hit.
    [Graphical view]
    SUPFAMiSSF57879. SSF57879. 1 hit.
    PROSITEiPS01119. COPPER_FIST_1. 1 hit.
    PS50073. COPPER_FIST_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12753-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVLINGIKYA CERCIRGHRV TTCNHTDQPL MMIKPKGRPS TTCDYCKQLR    50
    KNKNANPEGV CTCGRLEKKK LAQKAKEEAR AKAKEKQRKQ CTCGTDEVCK 100
    YHAQKRHLRK SPSSSQKKGR SISRSQPMFE RVLSSTSLDS NMLSGHGALS 150
    DTSSILTSTF LDSEPGVGKI SKDYHHVPSL ASISSLQSSQ SLDQNFSIPQ 200
    SPPLSSMSFN FLTGNINETN QNHSNHQHSK SGNNWQDSSV SLPAKADSRL 250
    NMMDKNNSVG LDLLGHSKRI SPISNSRVGE VSVPLEEYIP SDIDGVGRVT 300
    DKSSLVYDWP FDESIERNFS TTATAATGES KFDINDNCNR INSKSYSKTN 350
    SMNGNGMNNS NNNNINSNGN DKNNNNSSRQ EHQGNGLFDM FTDSSSISTL 400
    SRANLLLQEK IGSQENSVKQ ENYSKNPQLR HQLTSRSRSF IHHPANEYLK 450
    NTFGNSHSND IGKGVEVLSL TPSFMDIPEK ERETERSPSS NYITDRPFTR 500
    KPRSSSIDVN HRYPPMAPTT VATSPGALNN AVASNLDDQL SLTSLNSQPS 550
    SIANMMMDPS NLAEQSSIHS VPQSINSPRM PKTGSRQDKN IHTKKEERNP 600
    LNNIHDLSQL ENVPDEMNQM FSPPLKSMNR PDAIRENSSS SNFIIQGNSM 650
    ISTPSGRNDL PDTSPMSSIQ TASPPSQLLT DQGFADLDNF MSSL 694
    Length:694
    Mass (Da):76,671
    Last modified:November 1, 1996 - v1
    Checksum:i0A129F79B4E99CA8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U31900 Genomic DNA. Translation: AAA97587.1.
    Z71255 Genomic DNA. Translation: CAA95048.1.
    Z48951 Genomic DNA. Translation: CAA88786.1.
    Z49919 Genomic DNA. Translation: CAA90152.1.
    AY692804 Genomic DNA. Translation: AAT92823.1.
    BK006949 Genomic DNA. Translation: DAA11435.1.
    PIRiS59753.
    RefSeqiNP_015333.1. NM_001184105.1.

    Genome annotation databases

    EnsemblFungiiYPR008W; YPR008W; YPR008W.
    GeneIDi856117.
    KEGGisce:YPR008W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U31900 Genomic DNA. Translation: AAA97587.1 .
    Z71255 Genomic DNA. Translation: CAA95048.1 .
    Z48951 Genomic DNA. Translation: CAA88786.1 .
    Z49919 Genomic DNA. Translation: CAA90152.1 .
    AY692804 Genomic DNA. Translation: AAT92823.1 .
    BK006949 Genomic DNA. Translation: DAA11435.1 .
    PIRi S59753.
    RefSeqi NP_015333.1. NM_001184105.1.

    3D structure databases

    ProteinModelPortali Q12753.
    SMRi Q12753. Positions 1-42.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36186. 37 interactions.
    DIPi DIP-1462N.
    IntActi Q12753. 7 interactions.
    MINTi MINT-402478.
    STRINGi 4932.YPR008W.

    Proteomic databases

    MaxQBi Q12753.
    PaxDbi Q12753.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR008W ; YPR008W ; YPR008W .
    GeneIDi 856117.
    KEGGi sce:YPR008W.

    Organism-specific databases

    CYGDi YPR008w.
    SGDi S000006212. HAA1.

    Phylogenomic databases

    eggNOGi NOG71500.
    GeneTreei ENSGT00530000068810.
    OMAi CTCGRLE.
    OrthoDBi EOG7J9W29.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34170-MONOMER.

    Miscellaneous databases

    NextBioi 981192.

    Gene expression databases

    Genevestigatori Q12753.

    Family and domain databases

    Gene3Di 3.90.430.10. 1 hit.
    InterProi IPR001083. Cu_fist_DNA-bd.
    [Graphical view ]
    Pfami PF00649. Copper-fist. 1 hit.
    [Graphical view ]
    PRINTSi PR00617. COPPERFIST.
    SMARTi SM00412. Cu_FIST. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57879. SSF57879. 1 hit.
    PROSITEi PS01119. COPPER_FIST_1. 1 hit.
    PS50073. COPPER_FIST_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Haa1, a protein homologous to the copper-regulated transcription factor ace1, is a novel transcriptional activator."
      Keller G., Ray E., Brown P.O., Winge D.R.
      J. Biol. Chem. 276:38697-38702(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-241, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-125 AND SER-241, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231 AND SER-291, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHAA1_YEAST
    AccessioniPrimary (citable) accession number: Q12753
    Secondary accession number(s): D6W419
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 16, 2001
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 339 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3