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Q12753 (HAA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional activator HAA1
Gene names
Name:HAA1
Ordered Locus Names:YPR008W
ORF Names:LPZ8W, YP9531.01, YP9723.08
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length694 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates the transcription of a set of genes, many of which encode membrane proteins. Among the genes regulated are YGR138C and YRO2. Does not seem to be dependent on copper. Ref.4

Subcellular location

Nucleus Ref.4.

Miscellaneous

Present with 339 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 copper-fist DNA-binding domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

FUS1P117103EBI-8118,EBI-7179

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 694694Transcriptional activator HAA1
PRO_0000194933

Regions

DNA binding1 – 4040Copper-fist

Sites

Metal binding111Zinc By similarity
Metal binding141Zinc By similarity
Metal binding231Zinc By similarity
Metal binding251Zinc By similarity

Amino acid modifications

Modified residue1251Phosphoserine Ref.8
Modified residue2311Phosphoserine Ref.9
Modified residue2411Phosphoserine Ref.6 Ref.8
Modified residue2911Phosphoserine Ref.9

Sequences

Sequence LengthMass (Da)Tools
Q12753 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 0A129F79B4E99CA8

FASTA69476,671
        10         20         30         40         50         60 
MVLINGIKYA CERCIRGHRV TTCNHTDQPL MMIKPKGRPS TTCDYCKQLR KNKNANPEGV 

        70         80         90        100        110        120 
CTCGRLEKKK LAQKAKEEAR AKAKEKQRKQ CTCGTDEVCK YHAQKRHLRK SPSSSQKKGR 

       130        140        150        160        170        180 
SISRSQPMFE RVLSSTSLDS NMLSGHGALS DTSSILTSTF LDSEPGVGKI SKDYHHVPSL 

       190        200        210        220        230        240 
ASISSLQSSQ SLDQNFSIPQ SPPLSSMSFN FLTGNINETN QNHSNHQHSK SGNNWQDSSV 

       250        260        270        280        290        300 
SLPAKADSRL NMMDKNNSVG LDLLGHSKRI SPISNSRVGE VSVPLEEYIP SDIDGVGRVT 

       310        320        330        340        350        360 
DKSSLVYDWP FDESIERNFS TTATAATGES KFDINDNCNR INSKSYSKTN SMNGNGMNNS 

       370        380        390        400        410        420 
NNNNINSNGN DKNNNNSSRQ EHQGNGLFDM FTDSSSISTL SRANLLLQEK IGSQENSVKQ 

       430        440        450        460        470        480 
ENYSKNPQLR HQLTSRSRSF IHHPANEYLK NTFGNSHSND IGKGVEVLSL TPSFMDIPEK 

       490        500        510        520        530        540 
ERETERSPSS NYITDRPFTR KPRSSSIDVN HRYPPMAPTT VATSPGALNN AVASNLDDQL 

       550        560        570        580        590        600 
SLTSLNSQPS SIANMMMDPS NLAEQSSIHS VPQSINSPRM PKTGSRQDKN IHTKKEERNP 

       610        620        630        640        650        660 
LNNIHDLSQL ENVPDEMNQM FSPPLKSMNR PDAIRENSSS SNFIIQGNSM ISTPSGRNDL 

       670        680        690 
PDTSPMSSIQ TASPPSQLLT DQGFADLDNF MSSL 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Haa1, a protein homologous to the copper-regulated transcription factor ace1, is a novel transcriptional activator."
Keller G., Ray E., Brown P.O., Winge D.R.
J. Biol. Chem. 276:38697-38702(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-241, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-125 AND SER-241, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231 AND SER-291, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U31900 Genomic DNA. Translation: AAA97587.1.
Z71255 Genomic DNA. Translation: CAA95048.1.
Z48951 Genomic DNA. Translation: CAA88786.1.
Z49919 Genomic DNA. Translation: CAA90152.1.
AY692804 Genomic DNA. Translation: AAT92823.1.
BK006949 Genomic DNA. Translation: DAA11435.1.
PIRS59753.
RefSeqNP_015333.1. NM_001184105.1.

3D structure databases

ProteinModelPortalQ12753.
SMRQ12753. Positions 1-42.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36186. 37 interactions.
DIPDIP-1462N.
IntActQ12753. 7 interactions.
MINTMINT-402478.
STRING4932.YPR008W.

Proteomic databases

MaxQBQ12753.
PaxDbQ12753.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR008W; YPR008W; YPR008W.
GeneID856117.
KEGGsce:YPR008W.

Organism-specific databases

CYGDYPR008w.
SGDS000006212. HAA1.

Phylogenomic databases

eggNOGNOG71500.
GeneTreeENSGT00530000068810.
OMACTCGRLE.
OrthoDBEOG7J9W29.

Enzyme and pathway databases

BioCycYEAST:G3O-34170-MONOMER.

Gene expression databases

GenevestigatorQ12753.

Family and domain databases

Gene3D3.90.430.10. 1 hit.
InterProIPR001083. Cu_fist_DNA-bd.
[Graphical view]
PfamPF00649. Copper-fist. 1 hit.
[Graphical view]
PRINTSPR00617. COPPERFIST.
SMARTSM00412. Cu_FIST. 1 hit.
[Graphical view]
SUPFAMSSF57879. SSF57879. 1 hit.
PROSITEPS01119. COPPER_FIST_1. 1 hit.
PS50073. COPPER_FIST_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio981192.

Entry information

Entry nameHAA1_YEAST
AccessionPrimary (citable) accession number: Q12753
Secondary accession number(s): D6W419
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families