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Q12749

- SMC6_YEAST

UniProt

Q12749 - SMC6_YEAST

Protein

Structural maintenance of chromosomes protein 6

Gene

SMC6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks. Probably plays a role in structure.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi109 – 1168ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. damaged DNA binding Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. chromosome separation Source: SGD
    2. DNA repair Source: SGD
    3. double-strand break repair via homologous recombination Source: SGD
    4. resolution of recombination intermediates Source: SGD

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32449-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Structural maintenance of chromosomes protein 6
    Alternative name(s):
    DNA repair protein RHC18
    Rad18 homolog
    Gene namesi
    Name:SMC6
    Synonyms:RHC18
    Ordered Locus Names:YLR383W
    ORF Names:L3502.2
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR383w.
    SGDiS000004375. SMC6.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: SGD
    2. site of double-strand break Source: SGD
    3. Smc5-Smc6 complex Source: SGD

    Keywords - Cellular componenti

    Chromosome, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11141114Structural maintenance of chromosomes protein 6PRO_0000119022Add
    BLAST

    Proteomic databases

    MaxQBiQ12749.
    PaxDbiQ12749.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12749.

    Interactioni

    Subunit structurei

    Component of the Smc5-Smc6 complex which consists of KRE29, MMS21, NSE1, NSE3, NSE4, NSE5, SMC5 and SMC6.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    NSE1Q079134EBI-15099,EBI-30144

    Protein-protein interaction databases

    BioGridi31642. 83 interactions.
    DIPiDIP-6624N.
    IntActiQ12749. 5 interactions.
    MINTiMINT-675848.
    STRINGi4932.YLR383W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12749.
    SMRiQ12749. Positions 93-123, 1014-1082.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni530 – 695166Flexible hingeAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili259 – 529271Sequence AnalysisAdd
    BLAST
    Coiled coili696 – 969274Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi35 – 395Nuclear localization signalSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1019 – 105436Ala/Asp-rich (DA-box)Add
    BLAST

    Domaini

    The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC5, forming a V-shaped heterodimer.By similarity

    Sequence similaritiesi

    Belongs to the SMC family. SMC6 subfamily.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG1196.
    GeneTreeiENSGT00550000074816.
    HOGENOMiHOG000141857.
    OMAiDSPFRAM.
    OrthoDBiEOG7GXPKZ.

    Family and domain databases

    Gene3Di3.40.50.300. 3 hits.
    InterProiIPR027417. P-loop_NTPase.
    IPR027132. SMC6.
    [Graphical view]
    PANTHERiPTHR19306:SF2. PTHR19306:SF2. 1 hit.
    SUPFAMiSSF52540. SSF52540. 6 hits.

    Sequencei

    Sequence statusi: Complete.

    Q12749-1 [UniParc]FASTAAdd to Basket

    « Hide

    MISTTISGKR PIEQVDDELL SLTAQQENEE QQQQRKRRRH QFAPMTQFNS     50
    NTLDEDSGFR SSSDVATADQ DNFLEESPSG YIKKVILRNF MCHEHFELEL 100
    GSRLNFIVGN NGSGKSAILT AITIGLGAKA SETNRGSSLK DLIREGCYSA 150
    KIILHLDNSK YGAYQQGIFG NEIIVERIIK RDGPASFSLR SENGKEISNK 200
    KKDIQTVVDY FSVPVSNPMC FLSQDAARSF LTASTSQDKY SHFMKGTLLQ 250
    EITENLLYAS AIHDSAQENM ALHLENLKSL KAEYEDAKKL LRELNQTSDL 300
    NERKMLLQAK SLWIDVAHNT DACKNLENEI SGIQQKVDEV TEKIRNRQEK 350
    IERYTSDGTT IEAQIDAKVI YVNEKDSEHQ NARELLRDVK SRFEKEKSNQ 400
    AEAQSNIDQG RKKVDALNKT IAHLEEELTK EMGGDKDQMR QELEQLEKAN 450
    EKLREVNNSL VVSLQDVKNE ERDIQHERES ELRTISRSIQ NKKVELQNIA 500
    KGNDTFLMNF DRNMDRLLRT IEQRKNEFET PAIGPLGSLV TIRKGFEKWT 550
    RSIQRAISSS LNAFVVSNPK DNRLFRDIMR SCGIRSNIPI VTYCLSQFDY 600
    SKGRAHGNYP TIVDALEFSK PEIECLFVDL SRIERIVLIE DKNEARNFLQ 650
    RNPVNVNMAL SLRDRRSGFQ LSGGYRLDTV TYQDKIRLKV NSSSDNGTQY 700
    LKDLIEQETK ELQNIRDRYE EKLSEVRSRL KEIDGRLKST KNEMRKTNFR 750
    MTELKMNVGK VVDTGILNSK INERKNQEQA IASYEAAKEE LGLKIEQIAQ 800
    EAQPIKEQYD STKLALVEAQ DELQQLKEDI NSRQSKIQKY KDDTIYYEDK 850
    KKVYLENIKK IEVNVAALKE GIQRQIQNAC AFCSKERIEN VDLPDTQEEI 900
    KRELDKVSRM IQKAEKSLGL SQEEVIALFE KCRNKYKEGQ KKYMEIDEAL 950
    NRLHNSLKAR DQNYKNAEKG TCFDADMDFR ASLKVRKFSG NLSFIKDTKS 1000
    LEIYILTTND EKARNVDTLS GGEKSFSQMA LLLATWKPMR SRIIALDEFD 1050
    VFMDQVNRKI GTTLIVKKLK DIARTQTIII TPQDIGKIAD IDSSGVSIHR 1100
    MRDPERQNNS NFYN 1114
    Length:1,114
    Mass (Da):128,009
    Last modified:November 1, 1996 - v1
    Checksum:i55C166C5B72CD957
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80930 Genomic DNA. Translation: CAA56902.1.
    U19104 Genomic DNA. Translation: AAB67273.1.
    X05143 Genomic DNA. Translation: CAA28789.1.
    BK006945 Genomic DNA. Translation: DAA09684.1.
    PIRiS51470.
    RefSeqiNP_013487.1. NM_001182272.1.

    Genome annotation databases

    EnsemblFungiiYLR383W; YLR383W; YLR383W.
    GeneIDi851099.
    KEGGisce:YLR383W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80930 Genomic DNA. Translation: CAA56902.1 .
    U19104 Genomic DNA. Translation: AAB67273.1 .
    X05143 Genomic DNA. Translation: CAA28789.1 .
    BK006945 Genomic DNA. Translation: DAA09684.1 .
    PIRi S51470.
    RefSeqi NP_013487.1. NM_001182272.1.

    3D structure databases

    ProteinModelPortali Q12749.
    SMRi Q12749. Positions 93-123, 1014-1082.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31642. 83 interactions.
    DIPi DIP-6624N.
    IntActi Q12749. 5 interactions.
    MINTi MINT-675848.
    STRINGi 4932.YLR383W.

    Proteomic databases

    MaxQBi Q12749.
    PaxDbi Q12749.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR383W ; YLR383W ; YLR383W .
    GeneIDi 851099.
    KEGGi sce:YLR383W.

    Organism-specific databases

    CYGDi YLR383w.
    SGDi S000004375. SMC6.

    Phylogenomic databases

    eggNOGi COG1196.
    GeneTreei ENSGT00550000074816.
    HOGENOMi HOG000141857.
    OMAi DSPFRAM.
    OrthoDBi EOG7GXPKZ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32449-MONOMER.

    Miscellaneous databases

    NextBioi 967785.
    PROi Q12749.

    Gene expression databases

    Genevestigatori Q12749.

    Family and domain databases

    Gene3Di 3.40.50.300. 3 hits.
    InterProi IPR027417. P-loop_NTPase.
    IPR027132. SMC6.
    [Graphical view ]
    PANTHERi PTHR19306:SF2. PTHR19306:SF2. 1 hit.
    SUPFAMi SSF52540. SSF52540. 6 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The rad18 gene of Schizosaccharomyces pombe defines a new subgroup of the SMC superfamily involved in DNA repair."
      Lehmann A.R., Walicka M., Griffiths D.J.F., Murray J.M., Watts F.Z., McCready S., Carr A.M.
      Mol. Cell. Biol. 15:7067-7080(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Three suppressor mutations which cure a mitochondrial RNA maturase deficiency occur at the same codon in the open reading frame of the nuclear NAM2 gene."
      Labouesse M., Herbert C.J., Dujardin G., Slonimski P.P.
      EMBO J. 6:713-721(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-184.
      Strain: AB1-4A/8/55.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "SMC6 is required for MMS-induced interchromosomal and sister chromatid recombinations in Saccharomyces cerevisiae."
      Onoda F., Takeda M., Seki M., Maeda D., Tajima J., Ui A., Yagi H., Enomoto T.
      DNA Repair 3:429-439(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization."
      Zhao X., Blobel G.
      Proc. Natl. Acad. Sci. U.S.A. 102:4777-4782(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.

    Entry informationi

    Entry nameiSMC6_YEAST
    AccessioniPrimary (citable) accession number: Q12749
    Secondary accession number(s): D6VZ18
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 339 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3