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Q12749

- SMC6_YEAST

UniProt

Q12749 - SMC6_YEAST

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Protein
Structural maintenance of chromosomes protein 6
Gene
SMC6, RHC18, YLR383W, L3502.2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks. Probably plays a role in structure.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi109 – 1168ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. damaged DNA binding Source: SGD
  3. protein binding Source: IntAct

GO - Biological processi

  1. DNA repair Source: SGD
  2. chromosome separation Source: SGD
  3. double-strand break repair via homologous recombination Source: SGD
  4. resolution of recombination intermediates Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32449-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Structural maintenance of chromosomes protein 6
Alternative name(s):
DNA repair protein RHC18
Rad18 homolog
Gene namesi
Name:SMC6
Synonyms:RHC18
Ordered Locus Names:YLR383W
ORF Names:L3502.2
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR383w.
SGDiS000004375. SMC6.

Subcellular locationi

GO - Cellular componenti

  1. Smc5-Smc6 complex Source: SGD
  2. nucleus Source: SGD
  3. site of double-strand break Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11141114Structural maintenance of chromosomes protein 6
PRO_0000119022Add
BLAST

Proteomic databases

MaxQBiQ12749.
PaxDbiQ12749.

Expressioni

Gene expression databases

GenevestigatoriQ12749.

Interactioni

Subunit structurei

Component of the Smc5-Smc6 complex which consists of KRE29, MMS21, NSE1, NSE3, NSE4, NSE5, SMC5 and SMC6.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NSE1Q079134EBI-15099,EBI-30144

Protein-protein interaction databases

BioGridi31642. 83 interactions.
DIPiDIP-6624N.
IntActiQ12749. 5 interactions.
MINTiMINT-675848.
STRINGi4932.YLR383W.

Structurei

3D structure databases

ProteinModelPortaliQ12749.
SMRiQ12749. Positions 93-123, 1014-1082.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni530 – 695166Flexible hinge
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili259 – 529271 Reviewed prediction
Add
BLAST
Coiled coili696 – 969274 Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi35 – 395Nuclear localization signal Reviewed prediction

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1019 – 105436Ala/Asp-rich (DA-box)
Add
BLAST

Domaini

The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC5, forming a V-shaped heterodimer By similarity.

Sequence similaritiesi

Belongs to the SMC family. SMC6 subfamily.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG1196.
GeneTreeiENSGT00550000074816.
HOGENOMiHOG000141857.
OMAiDSPFRAM.
OrthoDBiEOG7GXPKZ.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR027417. P-loop_NTPase.
IPR027132. SMC6.
[Graphical view]
PANTHERiPTHR19306:SF2. PTHR19306:SF2. 1 hit.
SUPFAMiSSF52540. SSF52540. 6 hits.

Sequencei

Sequence statusi: Complete.

Q12749-1 [UniParc]FASTAAdd to Basket

« Hide

MISTTISGKR PIEQVDDELL SLTAQQENEE QQQQRKRRRH QFAPMTQFNS     50
NTLDEDSGFR SSSDVATADQ DNFLEESPSG YIKKVILRNF MCHEHFELEL 100
GSRLNFIVGN NGSGKSAILT AITIGLGAKA SETNRGSSLK DLIREGCYSA 150
KIILHLDNSK YGAYQQGIFG NEIIVERIIK RDGPASFSLR SENGKEISNK 200
KKDIQTVVDY FSVPVSNPMC FLSQDAARSF LTASTSQDKY SHFMKGTLLQ 250
EITENLLYAS AIHDSAQENM ALHLENLKSL KAEYEDAKKL LRELNQTSDL 300
NERKMLLQAK SLWIDVAHNT DACKNLENEI SGIQQKVDEV TEKIRNRQEK 350
IERYTSDGTT IEAQIDAKVI YVNEKDSEHQ NARELLRDVK SRFEKEKSNQ 400
AEAQSNIDQG RKKVDALNKT IAHLEEELTK EMGGDKDQMR QELEQLEKAN 450
EKLREVNNSL VVSLQDVKNE ERDIQHERES ELRTISRSIQ NKKVELQNIA 500
KGNDTFLMNF DRNMDRLLRT IEQRKNEFET PAIGPLGSLV TIRKGFEKWT 550
RSIQRAISSS LNAFVVSNPK DNRLFRDIMR SCGIRSNIPI VTYCLSQFDY 600
SKGRAHGNYP TIVDALEFSK PEIECLFVDL SRIERIVLIE DKNEARNFLQ 650
RNPVNVNMAL SLRDRRSGFQ LSGGYRLDTV TYQDKIRLKV NSSSDNGTQY 700
LKDLIEQETK ELQNIRDRYE EKLSEVRSRL KEIDGRLKST KNEMRKTNFR 750
MTELKMNVGK VVDTGILNSK INERKNQEQA IASYEAAKEE LGLKIEQIAQ 800
EAQPIKEQYD STKLALVEAQ DELQQLKEDI NSRQSKIQKY KDDTIYYEDK 850
KKVYLENIKK IEVNVAALKE GIQRQIQNAC AFCSKERIEN VDLPDTQEEI 900
KRELDKVSRM IQKAEKSLGL SQEEVIALFE KCRNKYKEGQ KKYMEIDEAL 950
NRLHNSLKAR DQNYKNAEKG TCFDADMDFR ASLKVRKFSG NLSFIKDTKS 1000
LEIYILTTND EKARNVDTLS GGEKSFSQMA LLLATWKPMR SRIIALDEFD 1050
VFMDQVNRKI GTTLIVKKLK DIARTQTIII TPQDIGKIAD IDSSGVSIHR 1100
MRDPERQNNS NFYN 1114
Length:1,114
Mass (Da):128,009
Last modified:November 1, 1996 - v1
Checksum:i55C166C5B72CD957
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X80930 Genomic DNA. Translation: CAA56902.1.
U19104 Genomic DNA. Translation: AAB67273.1.
X05143 Genomic DNA. Translation: CAA28789.1.
BK006945 Genomic DNA. Translation: DAA09684.1.
PIRiS51470.
RefSeqiNP_013487.1. NM_001182272.1.

Genome annotation databases

EnsemblFungiiYLR383W; YLR383W; YLR383W.
GeneIDi851099.
KEGGisce:YLR383W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X80930 Genomic DNA. Translation: CAA56902.1 .
U19104 Genomic DNA. Translation: AAB67273.1 .
X05143 Genomic DNA. Translation: CAA28789.1 .
BK006945 Genomic DNA. Translation: DAA09684.1 .
PIRi S51470.
RefSeqi NP_013487.1. NM_001182272.1.

3D structure databases

ProteinModelPortali Q12749.
SMRi Q12749. Positions 93-123, 1014-1082.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31642. 83 interactions.
DIPi DIP-6624N.
IntActi Q12749. 5 interactions.
MINTi MINT-675848.
STRINGi 4932.YLR383W.

Proteomic databases

MaxQBi Q12749.
PaxDbi Q12749.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR383W ; YLR383W ; YLR383W .
GeneIDi 851099.
KEGGi sce:YLR383W.

Organism-specific databases

CYGDi YLR383w.
SGDi S000004375. SMC6.

Phylogenomic databases

eggNOGi COG1196.
GeneTreei ENSGT00550000074816.
HOGENOMi HOG000141857.
OMAi DSPFRAM.
OrthoDBi EOG7GXPKZ.

Enzyme and pathway databases

BioCyci YEAST:G3O-32449-MONOMER.

Miscellaneous databases

NextBioi 967785.
PROi Q12749.

Gene expression databases

Genevestigatori Q12749.

Family and domain databases

Gene3Di 3.40.50.300. 3 hits.
InterProi IPR027417. P-loop_NTPase.
IPR027132. SMC6.
[Graphical view ]
PANTHERi PTHR19306:SF2. PTHR19306:SF2. 1 hit.
SUPFAMi SSF52540. SSF52540. 6 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The rad18 gene of Schizosaccharomyces pombe defines a new subgroup of the SMC superfamily involved in DNA repair."
    Lehmann A.R., Walicka M., Griffiths D.J.F., Murray J.M., Watts F.Z., McCready S., Carr A.M.
    Mol. Cell. Biol. 15:7067-7080(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Three suppressor mutations which cure a mitochondrial RNA maturase deficiency occur at the same codon in the open reading frame of the nuclear NAM2 gene."
    Labouesse M., Herbert C.J., Dujardin G., Slonimski P.P.
    EMBO J. 6:713-721(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-184.
    Strain: AB1-4A/8/55.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "SMC6 is required for MMS-induced interchromosomal and sister chromatid recombinations in Saccharomyces cerevisiae."
    Onoda F., Takeda M., Seki M., Maeda D., Tajima J., Ui A., Yagi H., Enomoto T.
    DNA Repair 3:429-439(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization."
    Zhao X., Blobel G.
    Proc. Natl. Acad. Sci. U.S.A. 102:4777-4782(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.

Entry informationi

Entry nameiSMC6_YEAST
AccessioniPrimary (citable) accession number: Q12749
Secondary accession number(s): D6VZ18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 339 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

Similar proteinsi