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Protein

Central kinetochore subunit CTF3

Gene

CTF3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation.

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: SGD
  • DNA replication initiation Source: SGD
  • establishment of mitotic sister chromatid cohesion Source: SGD
  • meiotic cell cycle Source: UniProtKB-KW
  • mitotic spindle assembly checkpoint Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-32447-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Central kinetochore subunit CTF3
Alternative name(s):
Chromosome loss protein 3
Chromosome transmission fidelity protein 3
Gene namesi
Name:CTF3
Synonyms:CHL3
Ordered Locus Names:YLR381W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR381W.
SGDiS000004373. CTF3.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome kinetochore Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000794932 – 733Central kinetochore subunit CTF3Add BLAST732

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ12748.
PRIDEiQ12748.

Interactioni

Subunit structurei

Heterotrimer. CTF3, MCM16 and MCM22 form the CTF3 kinetochore subcomplex. The CTF3 subcomplex is part of a larger central kinetochore CTF19 complex, which is composed of at least AME1, CHL4, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, NKP1, NKP2 and OKP1. Interacts with CHL4 and CTF19.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CTF19Q027323EBI-30457,EBI-5199
MCM16Q122623EBI-30457,EBI-31487
MCM22P471672EBI-30457,EBI-25691

Protein-protein interaction databases

BioGridi31640. 73 interactors.
DIPiDIP-4647N.
IntActiQ12748. 13 interactors.
MINTiMINT-544629.

Structurei

3D structure databases

ProteinModelPortaliQ12748.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000112042.
InParanoidiQ12748.
KOiK11501.
OMAiNYLWRNK.
OrthoDBiEOG092C33FE.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12748-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLILDDIIL SLTNANERTP PQALKTTLSL LYEKSKQYGL SSPQLQALVR
60 70 80 90 100
LLCETSIIDT VTKVYIVENC FLPDGYLTKE LLLEIINHLG TPTVFSRYRI
110 120 130 140 150
QTPPVLQSAL CKWLVHVYFL FPVHSEREHN ISSSIWLHLW QFSFLQKWIT
160 170 180 190 200
PLVIWQATTP VDVKPWKLSI IKRCAMHPGY RDAPGSATLI LQRFQCLVGA
210 220 230 240 250
SSQITESIIT INCNRKTLKS HRNLKLDAHF LSILKRILSR AHPANFPADT
260 270 280 290 300
VQNTIDMYLS EIHQLGADSI YPLRLQSLPE YVPSDSTVSL WDVTSLEQLA
310 320 330 340 350
QNWPQLHIPN DVDYMMKPSL NSNVLLPRKV MSRDSLKHLY SSIILIKNSR
360 370 380 390 400
DESSSPYEWC IWQLKRCFAH QIETPQEVIP IIISVSSMDN KLSSRIIQTF
410 420 430 440 450
CNLKYLKLDE LTLKKVCGGI LPLWKPELIS GTREFFVKFM ASIFMWSTRD
460 470 480 490 500
GHDNNCTFSE TCFYVLQMIT NWVLDDKLIA LGLTLLHDMQ SLLTLDKIFN
510 520 530 540 550
NATSNRFSTM AFISSLDILT QLSKQTKSDY AIQYLIVGPD IMNKVFSSDD
560 570 580 590 600
PLLLSAACRY LVATKNKLMQ YPSTNKFVRM QNQYIMDLTN YLYRNKVLSS
610 620 630 640 650
KSLFGVSPDF FKQILENLYI PTADFKNAKF FTITGIPALS YICIIILRRL
660 670 680 690 700
ETAENTKIKF TSGIINEETF NNFFRVHHDE IGQHGWIKGV NNIHDLRVKI
710 720 730
LMHLSNTANP YRDIAAFLFT YLKSLSKYSV QNS
Length:733
Mass (Da):84,252
Last modified:November 1, 1996 - v1
Checksum:i38DB89EE32C44AC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19104 Genomic DNA. Translation: AAB67274.1.
X05143 Genomic DNA. Translation: CAA28792.1.
BK006945 Genomic DNA. Translation: DAA09682.1.
PIRiS51468.
RefSeqiNP_013485.1. NM_001182270.1.

Genome annotation databases

EnsemblFungiiYLR381W; YLR381W; YLR381W.
GeneIDi851097.
KEGGisce:YLR381W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19104 Genomic DNA. Translation: AAB67274.1.
X05143 Genomic DNA. Translation: CAA28792.1.
BK006945 Genomic DNA. Translation: DAA09682.1.
PIRiS51468.
RefSeqiNP_013485.1. NM_001182270.1.

3D structure databases

ProteinModelPortaliQ12748.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31640. 73 interactors.
DIPiDIP-4647N.
IntActiQ12748. 13 interactors.
MINTiMINT-544629.

Proteomic databases

MaxQBiQ12748.
PRIDEiQ12748.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR381W; YLR381W; YLR381W.
GeneIDi851097.
KEGGisce:YLR381W.

Organism-specific databases

EuPathDBiFungiDB:YLR381W.
SGDiS000004373. CTF3.

Phylogenomic databases

HOGENOMiHOG000112042.
InParanoidiQ12748.
KOiK11501.
OMAiNYLWRNK.
OrthoDBiEOG092C33FE.

Enzyme and pathway databases

BioCyciYEAST:G3O-32447-MONOMER.

Miscellaneous databases

PROiQ12748.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiCTF3_YEAST
AccessioniPrimary (citable) accession number: Q12748
Secondary accession number(s): D6VZ16, Q7LHY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 319 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.