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Protein

Inner kinetochore subunit CTF3

Gene

CTF3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation.
Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.1 Publication

Miscellaneous

Present with 319 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: SGD
  • DNA replication initiation Source: SGD
  • establishment of mitotic sister chromatid cohesion Source: SGD
  • meiotic cell cycle Source: UniProtKB-KW
  • mitotic spindle assembly checkpoint Source: SGD

Keywordsi

Biological processCell cycle, Cell division, Meiosis, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-32447-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Inner kinetochore subunit CTF3Curated
Alternative name(s):
CENP-I homolog1 Publication
Chromosome loss protein 3
Chromosome transmission fidelity protein 3
Constitutive centromere-associated network protein CTF3Curated
Gene namesi
Name:CTF3
Synonyms:CHL3
Ordered Locus Names:YLR381W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR381W
SGDiS000004373 CTF3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000794932 – 733Inner kinetochore subunit CTF3Add BLAST732

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ12748
PaxDbiQ12748
PRIDEiQ12748

PTM databases

iPTMnetiQ12748

Interactioni

Subunit structurei

Component of the heterotrimeric kinetochore subcomplex CTF3, which consists of CTF3, MCM16 and MCM22 (PubMed:11782448). The CTF3 subcomplex is part of a larger constitutive centromere-associated network (CCAN) (also known as central kinetochore CTF19 complex in yeast), which is composed of at least AME1, CHL4, CNN1, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, MHF1, MHF2, MIF2, NKP1, NKP2, OKP1 and WIP1 (PubMed:12408861, PubMed:22561346). Interacts with CTF19 (PubMed:11782448, PubMed:12589047). Interacts with CHL4 (PubMed:12589047).4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31640, 145 interactors
DIPiDIP-4647N
IntActiQ12748, 13 interactors
MINTiQ12748
STRINGi4932.YLR381W

Structurei

3D structure databases

ProteinModelPortaliQ12748
SMRiQ12748
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CENP-I/CTF3 family.Curated

Phylogenomic databases

HOGENOMiHOG000112042
InParanoidiQ12748
KOiK11501
OMAiNYLWRNK
OrthoDBiEOG092C33FE

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12748-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLILDDIIL SLTNANERTP PQALKTTLSL LYEKSKQYGL SSPQLQALVR
60 70 80 90 100
LLCETSIIDT VTKVYIVENC FLPDGYLTKE LLLEIINHLG TPTVFSRYRI
110 120 130 140 150
QTPPVLQSAL CKWLVHVYFL FPVHSEREHN ISSSIWLHLW QFSFLQKWIT
160 170 180 190 200
PLVIWQATTP VDVKPWKLSI IKRCAMHPGY RDAPGSATLI LQRFQCLVGA
210 220 230 240 250
SSQITESIIT INCNRKTLKS HRNLKLDAHF LSILKRILSR AHPANFPADT
260 270 280 290 300
VQNTIDMYLS EIHQLGADSI YPLRLQSLPE YVPSDSTVSL WDVTSLEQLA
310 320 330 340 350
QNWPQLHIPN DVDYMMKPSL NSNVLLPRKV MSRDSLKHLY SSIILIKNSR
360 370 380 390 400
DESSSPYEWC IWQLKRCFAH QIETPQEVIP IIISVSSMDN KLSSRIIQTF
410 420 430 440 450
CNLKYLKLDE LTLKKVCGGI LPLWKPELIS GTREFFVKFM ASIFMWSTRD
460 470 480 490 500
GHDNNCTFSE TCFYVLQMIT NWVLDDKLIA LGLTLLHDMQ SLLTLDKIFN
510 520 530 540 550
NATSNRFSTM AFISSLDILT QLSKQTKSDY AIQYLIVGPD IMNKVFSSDD
560 570 580 590 600
PLLLSAACRY LVATKNKLMQ YPSTNKFVRM QNQYIMDLTN YLYRNKVLSS
610 620 630 640 650
KSLFGVSPDF FKQILENLYI PTADFKNAKF FTITGIPALS YICIIILRRL
660 670 680 690 700
ETAENTKIKF TSGIINEETF NNFFRVHHDE IGQHGWIKGV NNIHDLRVKI
710 720 730
LMHLSNTANP YRDIAAFLFT YLKSLSKYSV QNS
Length:733
Mass (Da):84,252
Last modified:November 1, 1996 - v1
Checksum:i38DB89EE32C44AC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19104 Genomic DNA Translation: AAB67274.1
X05143 Genomic DNA Translation: CAA28792.1
BK006945 Genomic DNA Translation: DAA09682.1
PIRiS51468
RefSeqiNP_013485.1, NM_001182270.1

Genome annotation databases

EnsemblFungiiYLR381W; YLR381W; YLR381W
GeneIDi851097
KEGGisce:YLR381W

Similar proteinsi

Entry informationi

Entry nameiCENPI_YEAST
AccessioniPrimary (citable) accession number: Q12748
Secondary accession number(s): D6VZ16, Q7LHY6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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