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Q12746

- PGA3_YEAST

UniProt

Q12746 - PGA3_YEAST

Protein

Plasma membrane-associated coenzyme Q6 reductase PGA3

Gene

PGA3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    NADH-dependent cytochrome b5 reductase that reduces coenzyme Q6 at the plasma membrane and mediates lifespan extension by calorie restriction by shifting fermentative to respiratory metabolism, probably through modulating the NAD+/NADH ratio.2 Publications

    Catalytic activityi

    NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.1 Publication

    Cofactori

    FAD.By similarity

    Enzyme regulationi

    Inhibited by diphenylene iodonium (DPI).1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi153 – 16816FADBy similarityAdd
    BLAST
    Nucleotide bindingi179 – 21133FADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. cytochrome-b5 reductase activity, acting on NAD(P)H Source: SGD

    GO - Biological processi

    1. chronological cell aging Source: SGD
    2. protein transport Source: SGD
    3. replicative cell aging Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NAD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32704-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Plasma membrane-associated coenzyme Q6 reductase PGA3 (EC:1.6.2.2)
    Alternative name(s):
    Processing of GAS1 and ALP protein 3
    Gene namesi
    Name:PGA3
    Synonyms:NQR1
    Ordered Locus Names:YML125C
    ORF Names:YM4987.10C, YM7056.01C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML125c.
    SGDiS000004594. PGA3.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 312312Plasma membrane-associated coenzyme Q6 reductase PGA3PRO_0000167628Add
    BLAST

    Proteomic databases

    MaxQBiQ12746.
    PaxDbiQ12746.
    PeptideAtlasiQ12746.

    Expressioni

    Inductioni

    By calorie restriction.1 Publication

    Gene expression databases

    GenevestigatoriQ12746.

    Interactioni

    Protein-protein interaction databases

    BioGridi35080. 216 interactions.
    DIPiDIP-4519N.
    IntActiQ12746. 3 interactions.
    MINTiMINT-521623.
    STRINGi4932.YML125C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12746.
    SMRiQ12746. Positions 62-296.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1515ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini37 – 393CytoplasmicSequence Analysis
    Topological domaini61 – 179119ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini201 – 312112CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei16 – 3621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei40 – 6021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei180 – 20021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini70 – 173104FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0543.
    GeneTreeiENSGT00390000008881.
    OMAiGRIDKEY.
    OrthoDBiEOG7N63XM.

    Family and domain databases

    InterProiIPR017927. Fd_Rdtase_FAD-bd.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR001834. NADH-Cyt_B5_reductase.
    IPR008333. OxRdtase_FAD-bd_dom.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00970. FAD_binding_6. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view]
    PRINTSiPR00406. CYTB5RDTASE.
    PR00371. FPNCR.
    SUPFAMiSSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12746-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKEDIEGTN ILDEPVHGIY IPAALFVVGV AITTYMSGEL KILWSLPILF    50
    MIIFVRAYSA YKRRRSLYPD RWTSLELEDQ TIISKNTALY RFKLKTRLES 100
    LDIPAGHHVA VRVPIDGKQE VRYYNPISSK LESGYLDLVV KAYVDGKVSK 150
    YFAGLNSGDT VDFKGPIGTL NYEPNSSKHL GIVAGGSGIT PVLQILNEII 200
    TVPEDLTKVS LLYANETEND ILLKDELDEM AEKYPHFQVH YVVHYPSDRW 250
    TGDVGYITKD QMNRYLPEYS EDNRLLICGP DGMNNLALQY AKELGWKVNS 300
    TRSSGDDQVF VF 312
    Length:312
    Mass (Da):35,287
    Last modified:November 1, 1997 - v1
    Checksum:i84C77D091F2FCA9B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49218 Genomic DNA. Translation: CAA89155.1.
    Z50178 Genomic DNA. Translation: CAA90558.1.
    BK006946 Genomic DNA. Translation: DAA09774.1.
    PIRiS54059.
    RefSeqiNP_013581.1. NM_001182488.1.

    Genome annotation databases

    EnsemblFungiiYML125C; YML125C; YML125C.
    GeneIDi854914.
    KEGGisce:YML125C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49218 Genomic DNA. Translation: CAA89155.1 .
    Z50178 Genomic DNA. Translation: CAA90558.1 .
    BK006946 Genomic DNA. Translation: DAA09774.1 .
    PIRi S54059.
    RefSeqi NP_013581.1. NM_001182488.1.

    3D structure databases

    ProteinModelPortali Q12746.
    SMRi Q12746. Positions 62-296.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35080. 216 interactions.
    DIPi DIP-4519N.
    IntActi Q12746. 3 interactions.
    MINTi MINT-521623.
    STRINGi 4932.YML125C.

    Proteomic databases

    MaxQBi Q12746.
    PaxDbi Q12746.
    PeptideAtlasi Q12746.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML125C ; YML125C ; YML125C .
    GeneIDi 854914.
    KEGGi sce:YML125C.

    Organism-specific databases

    CYGDi YML125c.
    SGDi S000004594. PGA3.

    Phylogenomic databases

    eggNOGi COG0543.
    GeneTreei ENSGT00390000008881.
    OMAi GRIDKEY.
    OrthoDBi EOG7N63XM.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32704-MONOMER.

    Miscellaneous databases

    NextBioi 977917.

    Gene expression databases

    Genevestigatori Q12746.

    Family and domain databases

    InterProi IPR017927. Fd_Rdtase_FAD-bd.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR001834. NADH-Cyt_B5_reductase.
    IPR008333. OxRdtase_FAD-bd_dom.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00970. FAD_binding_6. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00406. CYTB5RDTASE.
    PR00371. FPNCR.
    SUPFAMi SSF63380. SSF63380. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "A survey of essential gene function in the yeast cell division cycle."
      Yu L., Pena Castillo L., Mnaimneh S., Hughes T.R., Brown G.W.
      Mol. Biol. Cell 17:4736-4747(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    7. Cited for: INDUCTION, SUBCELLULAR LOCATION, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION.

    Entry informationi

    Entry nameiPGA3_YEAST
    AccessioniPrimary (citable) accession number: Q12746
    Secondary accession number(s): D6W0G0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 29000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3