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Q12746 (PGA3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Plasma membrane-associated coenzyme Q6 reductase PGA3

EC=1.6.2.2
Alternative name(s):
Processing of GAS1 and ALP protein 3
Gene names
Name:PGA3
Synonyms:NQR1
Ordered Locus Names:YML125C
ORF Names:YM4987.10C, YM7056.01C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length312 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

NADH-dependent cytochrome b5 reductase that reduces coenzyme Q6 at the plasma membrane and mediates lifespan extension by calorie restriction by shifting fermentative to respiratory metabolism, probably through modulating the NAD+/NADH ratio. Ref.5 Ref.7

Catalytic activity

NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5. Ref.7

Cofactor

FAD By similarity.

Enzyme regulation

Inhibited by diphenylene iodonium (DPI). Ref.7

Subcellular location

Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.3 Ref.7.

Induction

By calorie restriction. Ref.7

Miscellaneous

Present with 29000 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Contains 1 FAD-binding FR-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 312312Plasma membrane-associated coenzyme Q6 reductase PGA3
PRO_0000167628

Regions

Topological domain1 – 1515Extracellular Potential
Transmembrane16 – 3621Helical; Potential
Topological domain37 – 393Cytoplasmic Potential
Transmembrane40 – 6021Helical; Potential
Topological domain61 – 179119Extracellular Potential
Transmembrane180 – 20021Helical; Potential
Topological domain201 – 312112Cytoplasmic Potential
Domain70 – 173104FAD-binding FR-type
Nucleotide binding153 – 16816FAD By similarity
Nucleotide binding179 – 21133FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q12746 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 84C77D091F2FCA9B

FASTA31235,287
        10         20         30         40         50         60 
MSKEDIEGTN ILDEPVHGIY IPAALFVVGV AITTYMSGEL KILWSLPILF MIIFVRAYSA 

        70         80         90        100        110        120 
YKRRRSLYPD RWTSLELEDQ TIISKNTALY RFKLKTRLES LDIPAGHHVA VRVPIDGKQE 

       130        140        150        160        170        180 
VRYYNPISSK LESGYLDLVV KAYVDGKVSK YFAGLNSGDT VDFKGPIGTL NYEPNSSKHL 

       190        200        210        220        230        240 
GIVAGGSGIT PVLQILNEII TVPEDLTKVS LLYANETEND ILLKDELDEM AEKYPHFQVH 

       250        260        270        280        290        300 
YVVHYPSDRW TGDVGYITKD QMNRYLPEYS EDNRLLICGP DGMNNLALQY AKELGWKVNS 

       310 
TRSSGDDQVF VF 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"A survey of essential gene function in the yeast cell division cycle."
Yu L., Pena Castillo L., Mnaimneh S., Hughes T.R., Brown G.W.
Mol. Biol. Cell 17:4736-4747(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[7]"NQR1 controls lifespan by regulating the promotion of respiratory metabolism in yeast."
Jimenez-Hidalgo M., Santos-Ocana C., Padilla S., Villalba J.M., Lopez-Lluch G., Martin-Montalvo A., Minor R.K., Sinclair D.A., de Cabo R., Navas P.
Aging Cell 8:140-151(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, SUBCELLULAR LOCATION, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49218 Genomic DNA. Translation: CAA89155.1.
Z50178 Genomic DNA. Translation: CAA90558.1.
BK006946 Genomic DNA. Translation: DAA09774.1.
PIRS54059.
RefSeqNP_013581.1. NM_001182488.1.

3D structure databases

ProteinModelPortalQ12746.
SMRQ12746. Positions 62-296.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35080. 216 interactions.
DIPDIP-4519N.
IntActQ12746. 3 interactions.
MINTMINT-521623.
STRING4932.YML125C.

Proteomic databases

PaxDbQ12746.
PeptideAtlasQ12746.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML125C; YML125C; YML125C.
GeneID854914.
KEGGsce:YML125C.

Organism-specific databases

CYGDYML125c.
SGDS000004594. PGA3.

Phylogenomic databases

eggNOGCOG0543.
GeneTreeENSGT00390000008881.
OMANETKNDI.
OrthoDBEOG7N63XM.

Enzyme and pathway databases

BioCycYEAST:G3O-32704-MONOMER.

Gene expression databases

GenevestigatorQ12746.

Family and domain databases

InterProIPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR001834. NADH-Cyt_B5_reductase.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamPF00970. FAD_binding_6. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSPR00406. CYTB5RDTASE.
PR00371. FPNCR.
SUPFAMSSF63380. SSF63380. 1 hit.
PROSITEPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977917.

Entry information

Entry namePGA3_YEAST
AccessionPrimary (citable) accession number: Q12746
Secondary accession number(s): D6W0G0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 19, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families