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Protein

Plasma membrane-associated coenzyme Q6 reductase PGA3

Gene

PGA3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NADH-dependent cytochrome b5 reductase that reduces coenzyme Q6 at the plasma membrane and mediates lifespan extension by calorie restriction by shifting fermentative to respiratory metabolism, probably through modulating the NAD+/NADH ratio.2 Publications

Catalytic activityi

NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.1 Publication

Cofactori

FADBy similarity

Enzyme regulationi

Inhibited by diphenylene iodonium (DPI).1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi153 – 16816FADBy similarityAdd
BLAST
Nucleotide bindingi179 – 21133FADBy similarityAdd
BLAST

GO - Molecular functioni

  1. cytochrome-b5 reductase activity, acting on NAD(P)H Source: SGD

GO - Biological processi

  1. chronological cell aging Source: SGD
  2. protein transport Source: SGD
  3. replicative cell aging Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciYEAST:G3O-32704-MONOMER.
ReactomeiREACT_244882. Vitamin C (ascorbate) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane-associated coenzyme Q6 reductase PGA3 (EC:1.6.2.2)
Alternative name(s):
Processing of GAS1 and ALP protein 3
Gene namesi
Name:PGA3
Synonyms:NQR1
Ordered Locus Names:YML125C
ORF Names:YM4987.10C, YM7056.01C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML125c.
SGDiS000004594. PGA3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515ExtracellularSequence AnalysisAdd
BLAST
Transmembranei16 – 3621HelicalSequence AnalysisAdd
BLAST
Topological domaini37 – 393CytoplasmicSequence Analysis
Transmembranei40 – 6021HelicalSequence AnalysisAdd
BLAST
Topological domaini61 – 179119ExtracellularSequence AnalysisAdd
BLAST
Transmembranei180 – 20021HelicalSequence AnalysisAdd
BLAST
Topological domaini201 – 312112CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 312312Plasma membrane-associated coenzyme Q6 reductase PGA3PRO_0000167628Add
BLAST

Proteomic databases

MaxQBiQ12746.
PaxDbiQ12746.
PeptideAtlasiQ12746.

Expressioni

Inductioni

By calorie restriction.1 Publication

Gene expression databases

GenevestigatoriQ12746.

Interactioni

Protein-protein interaction databases

BioGridi35080. 216 interactions.
DIPiDIP-4519N.
IntActiQ12746. 3 interactions.
MINTiMINT-521623.
STRINGi4932.YML125C.

Structurei

3D structure databases

ProteinModelPortaliQ12746.
SMRiQ12746. Positions 62-296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini70 – 173104FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0543.
GeneTreeiENSGT00390000008881.
InParanoidiQ12746.
OMAiNETKNDI.
OrthoDBiEOG7N63XM.

Family and domain databases

InterProiIPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR001834. NADH-Cyt_B5_reductase.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00406. CYTB5RDTASE.
PR00371. FPNCR.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12746-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKEDIEGTN ILDEPVHGIY IPAALFVVGV AITTYMSGEL KILWSLPILF
60 70 80 90 100
MIIFVRAYSA YKRRRSLYPD RWTSLELEDQ TIISKNTALY RFKLKTRLES
110 120 130 140 150
LDIPAGHHVA VRVPIDGKQE VRYYNPISSK LESGYLDLVV KAYVDGKVSK
160 170 180 190 200
YFAGLNSGDT VDFKGPIGTL NYEPNSSKHL GIVAGGSGIT PVLQILNEII
210 220 230 240 250
TVPEDLTKVS LLYANETEND ILLKDELDEM AEKYPHFQVH YVVHYPSDRW
260 270 280 290 300
TGDVGYITKD QMNRYLPEYS EDNRLLICGP DGMNNLALQY AKELGWKVNS
310
TRSSGDDQVF VF
Length:312
Mass (Da):35,287
Last modified:November 1, 1997 - v1
Checksum:i84C77D091F2FCA9B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49218 Genomic DNA. Translation: CAA89155.1.
Z50178 Genomic DNA. Translation: CAA90558.1.
BK006946 Genomic DNA. Translation: DAA09774.1.
PIRiS54059.
RefSeqiNP_013581.1. NM_001182488.1.

Genome annotation databases

EnsemblFungiiYML125C; YML125C; YML125C.
GeneIDi854914.
KEGGisce:YML125C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49218 Genomic DNA. Translation: CAA89155.1.
Z50178 Genomic DNA. Translation: CAA90558.1.
BK006946 Genomic DNA. Translation: DAA09774.1.
PIRiS54059.
RefSeqiNP_013581.1. NM_001182488.1.

3D structure databases

ProteinModelPortaliQ12746.
SMRiQ12746. Positions 62-296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35080. 216 interactions.
DIPiDIP-4519N.
IntActiQ12746. 3 interactions.
MINTiMINT-521623.
STRINGi4932.YML125C.

Proteomic databases

MaxQBiQ12746.
PaxDbiQ12746.
PeptideAtlasiQ12746.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML125C; YML125C; YML125C.
GeneIDi854914.
KEGGisce:YML125C.

Organism-specific databases

CYGDiYML125c.
SGDiS000004594. PGA3.

Phylogenomic databases

eggNOGiCOG0543.
GeneTreeiENSGT00390000008881.
InParanoidiQ12746.
OMAiNETKNDI.
OrthoDBiEOG7N63XM.

Enzyme and pathway databases

BioCyciYEAST:G3O-32704-MONOMER.
ReactomeiREACT_244882. Vitamin C (ascorbate) metabolism.

Miscellaneous databases

NextBioi977917.

Gene expression databases

GenevestigatoriQ12746.

Family and domain databases

InterProiIPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR001834. NADH-Cyt_B5_reductase.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00406. CYTB5RDTASE.
PR00371. FPNCR.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "A survey of essential gene function in the yeast cell division cycle."
    Yu L., Pena Castillo L., Mnaimneh S., Hughes T.R., Brown G.W.
    Mol. Biol. Cell 17:4736-4747(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  7. Cited for: INDUCTION, SUBCELLULAR LOCATION, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION.

Entry informationi

Entry nameiPGA3_YEAST
AccessioniPrimary (citable) accession number: Q12746
Secondary accession number(s): D6W0G0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 29000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.