Plasma membrane-associated coenzyme Q6 reductase PGA3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q12746 (PGA3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Plasma membrane-associated coenzyme Q6 reductase PGA3
EC=1.6.-.-

Alternative name(s):
Processing of GAS1 and ALP protein 3

Gene names

Name:	PGA3
Synonyms:	NQR1
Ordered Locus Names:	YML125C
ORF Names:	YM4987.10C, YM7056.01C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	NADH-dependent cytochrome b5 reductase that reduces coenzyme Q6 at the plasma membrane and mediates lifespan extension by calorie restriction by shifting fermentative to respiratory metabolism, probably through modulating the NAD⁺/NADH ratio. Ref.5 Ref.7
Cofactor	FAD By similarity.
Enzyme regulation	Inhibited by diphenylene iodonium (DPI). Ref.7
Subcellular location	Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.3 Ref.7.
Induction	By calorie restriction. Ref.7
Miscellaneous	Present with 29000 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Cell membrane Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chronological cell aging Inferred from mutant phenotype Ref.7. Source: SGD protein transport Inferred from mutant phenotype Ref.5. Source: SGD replicative cell aging Inferred from mutant phenotype Ref.7. Source: SGD
Cellular_component	endoplasmic reticulum Inferred from direct assay Ref.3. Source: SGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay Ref.7. Source: SGD
Molecular_function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 312

312

Plasma membrane-associated coenzyme Q6 reductase PGA3

PRO_0000167628

Regions

Topological domain

1 – 15

Extracellular Potential

Transmembrane

16 – 36

Helical; Potential

Topological domain

37 – 39

Cytoplasmic Potential

Transmembrane

40 – 60

Helical; Potential

Topological domain

61 – 179

119

Extracellular Potential

Transmembrane

180 – 200

Helical; Potential

Topological domain

201 – 312

112

Cytoplasmic Potential

Domain

70 – 173

104

FAD-binding FR-type

Nucleotide binding

153 – 168

FAD By similarity

Nucleotide binding

179 – 211

FAD By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q12746 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 84C77D091F2FCA9B
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FASTA

312

35,287

        10         20         30         40         50         60 
MSKEDIEGTN ILDEPVHGIY IPAALFVVGV AITTYMSGEL KILWSLPILF MIIFVRAYSA 

        70         80         90        100        110        120 
YKRRRSLYPD RWTSLELEDQ TIISKNTALY RFKLKTRLES LDIPAGHHVA VRVPIDGKQE 

       130        140        150        160        170        180 
VRYYNPISSK LESGYLDLVV KAYVDGKVSK YFAGLNSGDT VDFKGPIGTL NYEPNSSKHL 

       190        200        210        220        230        240 
GIVAGGSGIT PVLQILNEII TVPEDLTKVS LLYANETEND ILLKDELDEM AEKYPHFQVH 

       250        260        270        280        290        300 
YVVHYPSDRW TGDVGYITKD QMNRYLPEYS EDNRLLICGP DGMNNLALQY AKELGWKVNS 

       310 
TRSSGDDQVF VF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A survey of essential gene function in the yeast cell division cycle." Yu L., Pena Castillo L., Mnaimneh S., Hughes T.R., Brown G.W. Mol. Biol. Cell 17:4736-4747(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[7]	"NQR1 controls lifespan by regulating the promotion of respiratory metabolism in yeast." Jimenez-Hidalgo M., Santos-Ocana C., Padilla S., Villalba J.M., Lopez-Lluch G., Martin-Montalvo A., Minor R.K., Sinclair D.A., de Cabo R., Navas P. Aging Cell 8:140-151(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION, SUBCELLULAR LOCATION, FUNCTION, ENZYME REGULATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49218 Genomic DNA. Translation: CAA89155.1. Z50178 Genomic DNA. Translation: CAA90558.1. BK006946 Genomic DNA. Translation: DAA09774.1.
PIR	S54059.
RefSeq	NP_013581.1. NM_001182488.1.
3D structure databases
ProteinModelPortal	Q12746.
SMR	Q12746. Positions 62-296.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4519N.
IntAct	Q12746. 3 interactions.
MINT	MINT-521623.
STRING	4932.YML125C.
Proteomic databases
PaxDb	Q12746.
PeptideAtlas	Q12746.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML125C; YML125C; YML125C.
GeneID	854914.
KEGG	sce:YML125C.
Organism-specific databases
CYGD	YML125c.
SGD	S000004594. PGA3.
Phylogenomic databases
eggNOG	COG0543.
GeneTree	ENSGT00390000008881.
OMA	YRFKLKT.
OrthoDB	EOG4BCHX2.
Gene expression databases
Genevestigator	Q12746.
GermOnline	YML125C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977917.

Entry information

Entry name

PGA3_YEAST

Accession

Primary (citable) accession number: Q12746
Secondary accession number(s): D6W0G0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents