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Protein

Protein transport protein SEC39

Gene

SEC39

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for protein transport between the Golgi and the endoplasmic reticulum. May contribute to tethering of coatomer-coated retrograde transport vesicles to the ER membrane through interaction with and stabilization of the SNARE complex.2 Publications

GO - Biological processi

  • ER-dependent peroxisome organization Source: SGD
  • protein transport Source: UniProtKB-KW
  • retrograde vesicle-mediated transport, Golgi to ER Source: SGD
  • vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32496-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein transport protein SEC39
Alternative name(s):
Dependent on SLY1-20 protein 3
Gene namesi
Name:SEC39Imported
Synonyms:DSL3
Ordered Locus Names:YLR440C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR440C.
SGDiS000004432. SEC39.

Subcellular locationi

GO - Cellular componenti

  • Dsl1p complex Source: SGD
  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: SGD
  • nuclear envelope Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 709709Protein transport protein SEC39PRO_0000234109Add
BLAST

Proteomic databases

MaxQBiQ12745.
PeptideAtlasiQ12745.

Interactioni

Subunit structurei

Component of a peripheral membrane protein complex consisting of DSL1, SEC39/DSL3 and TIP20. Bound to a SNARE complex consisting of UFE1, USE1, SEC20 and SEC22 or YKT6 through direct interaction of TIP20 with SEC20. Interacts with TIP20 and DSL1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DSL1P538478EBI-31898,EBI-29249
TIP20P338914EBI-31898,EBI-19396
USE1P531463EBI-31898,EBI-23881

Protein-protein interaction databases

BioGridi31699. 36 interactions.
DIPiDIP-941N.
IntActiQ12745. 16 interactions.
MINTiMINT-487138.

Structurei

Secondary structure

1
709
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi33 – 4210Combined sources
Helixi49 – 535Combined sources
Helixi54 – 607Combined sources
Helixi102 – 1043Combined sources
Helixi105 – 1117Combined sources
Turni112 – 1143Combined sources
Helixi120 – 13213Combined sources
Turni139 – 1424Combined sources
Helixi143 – 1475Combined sources
Helixi156 – 1649Combined sources
Helixi166 – 17510Combined sources
Helixi182 – 1876Combined sources
Helixi190 – 1978Combined sources
Helixi212 – 2165Combined sources
Helixi218 – 2258Combined sources
Helixi228 – 2358Combined sources
Turni238 – 2403Combined sources
Helixi246 – 26116Combined sources
Helixi270 – 28920Combined sources
Helixi294 – 30512Combined sources
Beta strandi310 – 3145Combined sources
Turni315 – 3173Combined sources
Beta strandi318 – 3214Combined sources
Helixi322 – 33413Combined sources
Helixi341 – 3488Combined sources
Helixi352 – 36716Combined sources
Helixi373 – 38210Combined sources
Turni383 – 3853Combined sources
Beta strandi392 – 3943Combined sources
Helixi399 – 41214Combined sources
Helixi416 – 42510Combined sources
Helixi432 – 44817Combined sources
Helixi457 – 47721Combined sources
Helixi479 – 49416Combined sources
Helixi515 – 5206Combined sources
Turni521 – 5233Combined sources
Helixi526 – 53510Combined sources
Helixi539 – 5413Combined sources
Helixi543 – 55614Combined sources
Helixi565 – 58218Combined sources
Helixi586 – 60318Combined sources
Helixi605 – 62420Combined sources
Helixi631 – 6333Combined sources
Helixi637 – 65317Combined sources
Helixi656 – 6583Combined sources
Helixi659 – 67315Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3K8PX-ray2.60D1-709[»]
ProteinModelPortaliQ12745.
SMRiQ12745. Positions 30-684.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12745.

Family & Domainsi

Sequence similaritiesi

Belongs to the SEC39 family.Curated

Phylogenomic databases

InParanoidiQ12745.
OMAiIFQVGKY.
OrthoDBiEOG7CRTZJ.

Family and domain databases

InterProiIPR013244. Sec39_domain.
[Graphical view]
PfamiPF08314. Sec39. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12745-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEEQLYLLA CIFASRADTR NIKKLSTRLG SQSKYLEILC VLWPELDDPK
60 70 80 90 100
NLLFLRELEE EVQSPEGEET TDEDVIVELL ESDSSLIPLI ESDTTTRSNR
110 120 130 140 150
YHELQEFISK KLNNKTLENF EEWLRERILI CNEMIPETPL LYSVLWETAK
160 170 180 190 200
SKVLSTKFIG WVEGVLKPLD HLNKRLHLIF KINEWEKMPD SELFKIIFDG
210 220 230 240 250
VEDMQGYIGI ADVIEDELAP TLSYGKKWET FITEFFNKQQ FSLKSDTNYQ
260 270 280 290 300
LFIKLYYSLE KGVKDNSEAS RKLQSNVVDI LFHNSENLFN LSSLTHKLDE
310 320 330 340 350
LWSILSGFPD EITIEEQKTI TALEMKQFME FFIKCSTKFS FKEIFAITQE
360 370 380 390 400
EESAQLAHFS SLCHEEFNKA NEISSFLQAM YETVLDISKD DKIFTRISMD
410 420 430 440 450
EKLYSILEIL LQMNEFAYIE AIIERFDYSN NTQIYELLVK FFWHFFNNAS
460 470 480 490 500
NGLRKEPEMK KASQTLQIIQ KHMSQRAGTN LTKLEVLLEI SDKLSHYSIN
510 520 530 540 550
LNKSHNGARD TAFKPSNILE YRDCPLDIIS NLLELNPRLY KDLPTTKSLL
560 570 580 590 600
FGIYDSLSIN REGQTGKVEV DLMVLHIDYA LVNLDFGTAY ELGKQVFEIC
610 620 630 640 650
QEAGQHMMKA LGDEHWLTFY QMGKFVDPNW VDNEIPTEII VLQMSILGRL
660 670 680 690 700
LEVCPLEEVE IVTSQWSTLE LELSARDLVK DKYALDGQND NKSKVGGIAR

EIFHNVTNF
Length:709
Mass (Da):82,396
Last modified:November 1, 1996 - v1
Checksum:i8ABD59328A006863
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21094 Genomic DNA. Translation: AAB67520.1.
Z30582 Genomic DNA. Translation: CAA83058.1.
BK006945 Genomic DNA. Translation: DAA09741.1.
PIRiS59408.
RefSeqiNP_013545.3. NM_001182328.3.

Genome annotation databases

EnsemblFungiiYLR440C; YLR440C; YLR440C.
GeneIDi851161.
KEGGisce:YLR440C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21094 Genomic DNA. Translation: AAB67520.1.
Z30582 Genomic DNA. Translation: CAA83058.1.
BK006945 Genomic DNA. Translation: DAA09741.1.
PIRiS59408.
RefSeqiNP_013545.3. NM_001182328.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3K8PX-ray2.60D1-709[»]
ProteinModelPortaliQ12745.
SMRiQ12745. Positions 30-684.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31699. 36 interactions.
DIPiDIP-941N.
IntActiQ12745. 16 interactions.
MINTiMINT-487138.

Proteomic databases

MaxQBiQ12745.
PeptideAtlasiQ12745.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR440C; YLR440C; YLR440C.
GeneIDi851161.
KEGGisce:YLR440C.

Organism-specific databases

EuPathDBiFungiDB:YLR440C.
SGDiS000004432. SEC39.

Phylogenomic databases

InParanoidiQ12745.
OMAiIFQVGKY.
OrthoDBiEOG7CRTZJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-32496-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ12745.
NextBioi967954.
PROiQ12745.

Family and domain databases

InterProiIPR013244. Sec39_domain.
[Graphical view]
PfamiPF08314. Sec39. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Gene MRP-L4, encoding mitochondrial ribosomal protein YmL4, is indispensable for proper non-respiratory cell functions in yeast."
    Graack H.-R., Grohmann L., Kitakawa M., Goldschmidt-Reisin S.
    Gene 152:107-112(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 548-709.
    Strain: 07173Imported.
  4. Cited for: FUNCTION.
  5. "Isolation of Saccharomyces cerevisiae RNase T1 hypersensitive (rns) mutants and genetic analysis of the RNS1/DSL1 gene."
    Ishikawa T., Unno K., Nonaka G., Nakajima H., Kitamoto K.
    J. Gen. Appl. Microbiol. 51:73-82(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TIP20.
  6. "Dsl1p, Tip20p, and the novel Dsl3(Sec39) protein are required for the stability of the Q/t-SNARE complex at the endoplasmic reticulum in yeast."
    Kraynack B.A., Chan A., Rosenthal E., Essid M., Umansky B., Waters M.G., Schmitt H.D.
    Mol. Biol. Cell 16:3963-3977(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN A COMPLEX WITH DSL1; TIP20; SEC20; USE1 AND UFE1, SUBCELLULAR LOCATION.
  7. Cited for: IDENTIFICATION IN A COMPLEX WITH DSL1; TIP20; SEC20; UFE1; USE1 AND SEC22.

Entry informationi

Entry nameiSEC39_YEAST
AccessioniPrimary (citable) accession number: Q12745
Secondary accession number(s): D6VZ75, Q7LHI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Cells with a reduced level of SEC39 show a defect in post-translational processing of PRC1/CPY.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.