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Q12739

- LAC2_PLEOS

UniProt

Q12739 - LAC2_PLEOS

Protein

Laccase-2

Gene

POX2

Organism
Pleurotus ostreatus (Oyster mushroom) (White-rot fungus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Lignin degradation and detoxification of lignin-derived products.By similarity

    Catalytic activityi

    4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

    Cofactori

    Binds 4 copper ions per monomer.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi98 – 981Copper 1; type 2By similarity
    Metal bindingi100 – 1001Copper 2; type 3By similarity
    Metal bindingi143 – 1431Copper 2; type 3By similarity
    Metal bindingi145 – 1451Copper 3; type 3By similarity
    Metal bindingi427 – 4271Copper 4; type 1By similarity
    Metal bindingi430 – 4301Copper 1; type 2By similarity
    Metal bindingi432 – 4321Copper 3; type 3By similarity
    Metal bindingi483 – 4831Copper 3; type 3By similarity
    Metal bindingi484 – 4841Copper 4; type 1By similarity
    Metal bindingi485 – 4851Copper 2; type 3By similarity
    Metal bindingi489 – 4891Copper 4; type 1By similarity

    GO - Molecular functioni

    1. copper ion binding Source: InterPro
    2. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. lignin catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lignin degradation

    Keywords - Ligandi

    Copper, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Laccase-2 (EC:1.10.3.2)
    Alternative name(s):
    Benzenediol:oxygen oxidoreductase 2
    Diphenol oxidase 2
    Urishiol oxidase 2
    Gene namesi
    Name:POX2
    OrganismiPleurotus ostreatus (Oyster mushroom) (White-rot fungus)
    Taxonomic identifieri5322 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesPleurotaceaePleurotus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Add
    BLAST
    Chaini24 – 533510Laccase-2PRO_0000002930Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi467 – 4671N-linked (GlcNAc...) (high mannose)1 Publication

    Post-translational modificationi

    N-glycosylated at Asn-467; contains a high-mannose glycan with a varying number of mannose residues.1 Publication

    Keywords - PTMi

    Glycoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliQ12739.
    SMRiQ12739. Positions 24-527.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 171147Plastocyanin-like 1Add
    BLAST
    Domaini173 – 336164Plastocyanin-like 2Add
    BLAST
    Domaini382 – 501120Plastocyanin-like 3Add
    BLAST

    Sequence similaritiesi

    Belongs to the multicopper oxidase family.Curated
    Contains 3 plastocyanin-like domains.Curated

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    Gene3Di2.60.40.420. 3 hits.
    InterProiIPR001117. Cu-oxidase.
    IPR011706. Cu-oxidase_2.
    IPR011707. Cu-oxidase_3.
    IPR002355. Cu_oxidase_Cu_BS.
    IPR008972. Cupredoxin.
    [Graphical view]
    PfamiPF00394. Cu-oxidase. 1 hit.
    PF07731. Cu-oxidase_2. 1 hit.
    PF07732. Cu-oxidase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF49503. SSF49503. 3 hits.
    PROSITEiPS00079. MULTICOPPER_OXIDASE1. 2 hits.
    PS00080. MULTICOPPER_OXIDASE2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q12739-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFPGARILAT LTLALHLLHG AHAAIGPAGN MYIVNEDVSP DGFARSAVVA    50
    RSVPATDPTP ATASIPGVLV QGNKGDNFQL NVVNQLSDTT MLKTTSIHWH 100
    GFFQAGSSWA DGPAFVTQCP VASGDSFLYN FNVPDQAGTF WYHSHLSTQY 150
    CDGLRGPFVV YDPSDPHLSL YDIDNADTVI TLEDWYHIVA PQNAAIPTPD 200
    STLINGKGRY AGGPTSPLAI INVESNKRYR FRLVSMSCDP NFTFSIDGHS 250
    LLVIEADAVN IVPITVDSIQ IFAGQRYSFV LTANQAVDNY WIRANPNLGS 300
    TGFVGGINSA ILRYAGATED DPTTTSSTST PLLETNLVPL ENPGAPGPPV 350
    PGGADININL AMAFDFTTFE LTINGVPFLP PTAPVLLQIL SGASTAASLL 400
    PSGSIYELEA NKVVEISMPA LAVGGPHPFH LHGHTFDVIR SAGSTTYNFD 450
    TPARRDVVNT GTGANDNVTI RFVTDNPGPW FLHCHIDWHL EIGLAVVFAE 500
    DVTSISAPPA AWDDLCPIYN ALSDNDKGGI VPS 533
    Length:533
    Mass (Da):56,767
    Last modified:November 1, 1996 - v1
    Checksum:i7233C41D47E19AE6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49075 Genomic DNA. Translation: CAA88895.1.
    Z34848 mRNA. Translation: CAA84357.1.
    PIRiS62371.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49075 Genomic DNA. Translation: CAA88895.1 .
    Z34848 mRNA. Translation: CAA84357.1 .
    PIRi S62371.

    3D structure databases

    ProteinModelPortali Q12739.
    SMRi Q12739. Positions 24-527.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.40.420. 3 hits.
    InterProi IPR001117. Cu-oxidase.
    IPR011706. Cu-oxidase_2.
    IPR011707. Cu-oxidase_3.
    IPR002355. Cu_oxidase_Cu_BS.
    IPR008972. Cupredoxin.
    [Graphical view ]
    Pfami PF00394. Cu-oxidase. 1 hit.
    PF07731. Cu-oxidase_2. 1 hit.
    PF07732. Cu-oxidase_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49503. SSF49503. 3 hits.
    PROSITEi PS00079. MULTICOPPER_OXIDASE1. 2 hits.
    PS00080. MULTICOPPER_OXIDASE2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The gene, protein and glycan structures of laccase from Pleurotus ostreatus."
      Giardina P., Aurilia V., Cannio R., Marzullo L., Amoresano A., Siciliano R., Pucci P., Sannia G.
      Eur. J. Biochem. 235:508-515(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], GLYCOSYLATION AT ASN-467, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Mycelium.
    2. "Stability and activity of a phenol oxidase from the ligninolytic fungus Pleurotus ostreatus."
      Palmieri G., Giardina P., Marzullo L., Desiderio B., Nitti G., Cannio R., Sannia G.
      Appl. Microbiol. Biotechnol. 39:632-636(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: Florida.
      Tissue: Mycelium.

    Entry informationi

    Entry nameiLAC2_PLEOS
    AccessioniPrimary (citable) accession number: Q12739
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    POX2 isozyme is the most abundant laccase of P.ostreatus under various growth conditions.

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3