Reviewed, UniProtKB/Swiss-Prot Q12732 (AVNA_ASPPA)

Names and origin

Protein names

Recommended name:
    Averantin oxidoreductase
    EC=1.14.-.-
Alternative name(s):
    Cytochrome P450 60A1

Gene names

Name:	avnA
Synonyms:	aflG, cyp60a1, ord-1, ord1

Organism

Aspergillus parasiticus

Taxonomic identifier

5067 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the hydroxylation of averantin to averufin.
Cofactor	Heme group By similarity.
Pathway	Mycotoxin biosynthesis; aflatoxin biosynthesis.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 495	495	Averantin oxidoreductase		PRO_0000052047
Sites
	Metal binding	436	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	168 – 170	3	PLD → SG in AAB52228. Ref.3
	Sequence conflict	168 – 170	3	PLD → SG in AAA87598. Ref.3
	Sequence conflict	307	1	G → I in AAB52228. Ref.3
	Sequence conflict	307	1	G → I in AAA87598. Ref.3
	Sequence conflict	484 – 495	12	KPLWV…EPRNE → NRCGLRWNLAMSSSS Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q12732-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 9060CC6A38FBB904	FASTA	495	56,345
	10 20 30 40 50 60 MGGDGWPSDG HILLLIVLTV LTPPSLALYR LWIHPLRSYP GPRWWAIWRG PYILSNIRGN 70 80 90 100 110 120 LVRDLQRLHQ QFGPVVRIAP NELSFIVPEA ASPIYTSNPE FPKDPMHLPP FHNGTPGILA 130 140 150 160 170 180 ADHAHHRRYR RLLAFSFSDK GLRHERSLIE RSIDLLITQL HENCGQGPLD LALWFNWATF 190 200 210 220 230 240 DIIGDLAFGD SFGCLENVQT HPWIASIQGN VKLIPILNAF RRYRLDGLLR LLGSRKLLEQ 250 260 270 280 290 300 RRRNAQFTTD QVDRRLKNSS TPRGDIWDAV LAQKPDGEPP MTRDEMISNA SAIVLAGSET 310 320 330 340 350 360 SATLLSGCTW LLLKNPSHLH QLTSRIRSQF THASEIDSQS VSRVEGLQAV LEESLRLYPP 370 380 390 400 410 420 VPMQSNRIVP QAGAYIAGGW VPGGTSVGLQ QFVACRSSSN FHRPDEFLPE RWQGQGEFAH 430 440 450 460 470 480 DRREVSQPFS IGPRNCIGRQ LAYVEMRLIL VKLLWHFDLR LDTTRMKDTD WLAEQGIWIL 490 WDKKPLWVTL EPRNE

References

[1]	"avnA, a gene encoding a cytochrome P-450 monooxygenase, is involved in the conversion of averantin to averufin in aflatoxin biosynthesis in Aspergillus parasiticus." Yu J., Chang P.-K., Cary J.W., Bhatnagar D., Cleveland T.E. Appl. Environ. Microbiol. 63:1349-1356(1997) [PubMed: 9097431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, CHARACTERIZATION. Strain: ATCC 56775 / NRRL 5862 / Su-1 / SRRC 143.
[2]	"Completed sequence of aflatoxin pathway gene cluster in Aspergillus parasiticus." Yu J., Bhatnagar D., Cleveland T.E. FEBS Lett. 564:126-130(2004) [PubMed: 15094053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 56775 / NRRL 5862 / Su-1 / SRRC 143.
[3]	"Comparative mapping of aflatoxin pathway gene clusters in Aspergillus parasiticus and Aspergillus flavus." Yu J., Chang P.-K., Cary J.W., Wright M., Bhatnagar D., Cleveland T.E., Payne G.A., Linz J.E. Appl. Environ. Microbiol. 61:2365-2371(1995) [PubMed: 7793957] [Abstract] Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [MRNA] OF 106-495. Strain: ATCC 56775 / NRRL 5862 / Su-1 / SRRC 143.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY371490 Genomic DNA. Translation: AAS66008.1. L40839 mRNA. Translation: AAB52228.1. L41149 mRNA. Translation: AAA87598.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-14028.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AVNA_ASPPA

Accession

Primary (citable) accession number: Q12732
Secondary accession number(s): O43104

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 15, 1998
Last modified:	June 16, 2009
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)