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Reviewed, UniProtKB/Swiss-Prot Q12729 (LAC1_PLEOS)

Last modified November 25, 2008. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Laccase-1
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 1
    Urishiol oxidase 1
    Diphenol oxidase 1
Gene names
Name: POX1
OrganismPleurotus ostreatus (Oyster mushroom) (White-rot fungus)
Taxonomic identifier5322 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Pleurotaceae › Pleurotus

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Protein attributes

Sequence length529 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Lignin degradation and detoxification of lignin-derived products By similarity.

Catalytic activity

4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O.

Cofactor

Binds 4 copper ions per monomer By similarity.

Subcellular location

Secreted.

Sequence similarities

Belongs to the multicopper oxidase family.

Contains 3 plastocyanin-like domains.

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Ontologies

Keywords

   Biological processLignin degradation
   Cellular componentSecreted
   DomainRepeat
Signal
   LigandCopper
Metal-binding
   Molecular functionOxidoreductase
   PTMGlycoprotein

Gene Ontology (GO)

   Biological processlignin catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functioncopper ion binding

Inferred from electronic annotation. Source: InterPro

laccase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 529506Laccase-1
PRO_0000002929

Regions

Domain25 – 159135Plastocyanin-like 1
Domain170 – 312143Plastocyanin-like 2
Domain380 – 499120Plastocyanin-like 3

Sites

Metal binding961Copper 1; type 2 By similarity
Metal binding981Copper 2; type 3 By similarity
Metal binding1411Copper 2; type 3 By similarity
Metal binding1431Copper 3; type 3 By similarity
Metal binding4251Copper 4; type 1 By similarity
Metal binding4281Copper 1; type 2 By similarity
Metal binding4301Copper 3; type 3 By similarity
Metal binding4811Copper 3; type 3 By similarity
Metal binding4821Copper 4; type 1 By similarity
Metal binding4831Copper 2; type 3 By similarity
Metal binding4871Copper 4; type 1 By similarity

Amino acid modifications

Glycosylation571N-linked (GlcNAc...) Potential
Glycosylation2391N-linked (GlcNAc...) Potential
Glycosylation2821N-linked (GlcNAc...) Potential
Glycosylation4651N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
Q12729-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 40110AFC4886882F

FASTA52956,580
        10         20         30         40         50         60 
MFPGARILAT LTLALHLLHG THAAIGPTGD MYIVNEDVSP DGFTRSAVVA RSDPTTNGTS 

        70         80         90        100        110        120 
ETLTGVLVQG NKGDNFQLNV LNQLSDTTML KTTSIHWHGF FQSGSTWADG PAFVNQCPIA 

       130        140        150        160        170        180 
SGNSFLYDFN VPDQAGTFWY HSHLSTQYCD GLRGPFIVYD PSDPHLSLYD VDNADTIITL 

       190        200        210        220        230        240 
EDWYHVVAPQ NAVLPTADST LINGKGRFAG GPTSALAVIN VESNKRYRFR LISMSCDPNF 

       250        260        270        280        290        300 
TFSIDGHSLQ VIEADAVNIV PIVVDSIQIF AGQRYSFVLN ANQTVDNYWI RADPNLGSTG 

       310        320        330        340        350        360 
FDGGINSAIL RYAGATEDDP TTTSSTSTPL EETNLVPLEN PGAPGPAVPG GADININLAM 

       370        380        390        400        410        420 
AFDVTNFELT INGSPFKAPT APVLLQILSG ATTAASLLPS GSIYSLEANK VVEISIPALA 

       430        440        450        460        470        480 
VGGPHPFHLH GHTFDVIRSA GSTTYNFDTP ARRDVVNTGT DANDNVTIRF VTDNPGPWFL 

       490        500        510        520 
HCHIDWHLEI GLAVVFAEDV TSITAPPAAW DDLCPIYDAL SDSDKGGIA

« Hide

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References

[1]"Cloning and sequencing of a laccase gene from the lignin-degrading basidiomycete Pleurotus ostreatus."
Giardina P., Cannio R., Martirani L., Marzullo L., Palmieri G., Sannia G.
Appl. Environ. Microbiol. 61:2408-2413(1995) [PubMed: 7793961] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: Florida.
Tissue: Mycelium.

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Cross-references

Sequence databases

Z34847 mRNA. Translation: CAA84356.1.
Z22591 Genomic DNA. Translation: CAA80305.1.
PIRS49120.

3D structure databases

HSSPHSSP built from PDB template 1GYC based on UniProtKB Q12718.
ModBaseSearch...

Family and domain databases

InterProIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
[Graphical view]
Gene3DG3DSA:2.60.40.420. Cupredoxin. 3 hits.
PfamPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
PROSITEPS00079. MULTICOPPER_OXIDASE1. 2 hits.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameLAC1_PLEOS
AccessionPrimary (citable) accession number: Q12729
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 25, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents