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Q12729

- LAC1_PLEOS

UniProt

Q12729 - LAC1_PLEOS

Protein

Laccase-1

Gene

POX1

Organism
Pleurotus ostreatus (Oyster mushroom) (White-rot fungus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Lignin degradation and detoxification of lignin-derived products.By similarity

    Catalytic activityi

    4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

    Cofactori

    Binds 4 copper ions per monomer.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi96 – 961Copper 1; type 2By similarity
    Metal bindingi98 – 981Copper 2; type 3By similarity
    Metal bindingi141 – 1411Copper 2; type 3By similarity
    Metal bindingi143 – 1431Copper 3; type 3By similarity
    Metal bindingi425 – 4251Copper 4; type 1By similarity
    Metal bindingi428 – 4281Copper 1; type 2By similarity
    Metal bindingi430 – 4301Copper 3; type 3By similarity
    Metal bindingi481 – 4811Copper 3; type 3By similarity
    Metal bindingi482 – 4821Copper 4; type 1By similarity
    Metal bindingi483 – 4831Copper 2; type 3By similarity
    Metal bindingi487 – 4871Copper 4; type 1By similarity

    GO - Molecular functioni

    1. copper ion binding Source: InterPro
    2. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. lignin catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lignin degradation

    Keywords - Ligandi

    Copper, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Laccase-1 (EC:1.10.3.2)
    Alternative name(s):
    Benzenediol:oxygen oxidoreductase 1
    Diphenol oxidase 1
    Urishiol oxidase 1
    Gene namesi
    Name:POX1
    OrganismiPleurotus ostreatus (Oyster mushroom) (White-rot fungus)
    Taxonomic identifieri5322 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesPleurotaceaePleurotus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 529506Laccase-1PRO_0000002929Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi57 – 571N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi239 – 2391N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi282 – 2821N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi465 – 4651N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliQ12729.
    SMRiQ12729. Positions 24-525.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 159135Plastocyanin-like 1Add
    BLAST
    Domaini170 – 312143Plastocyanin-like 2Add
    BLAST
    Domaini380 – 499120Plastocyanin-like 3Add
    BLAST

    Sequence similaritiesi

    Belongs to the multicopper oxidase family.Curated
    Contains 3 plastocyanin-like domains.Curated

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    Gene3Di2.60.40.420. 3 hits.
    InterProiIPR001117. Cu-oxidase.
    IPR011706. Cu-oxidase_2.
    IPR011707. Cu-oxidase_3.
    IPR002355. Cu_oxidase_Cu_BS.
    IPR008972. Cupredoxin.
    [Graphical view]
    PfamiPF00394. Cu-oxidase. 1 hit.
    PF07731. Cu-oxidase_2. 1 hit.
    PF07732. Cu-oxidase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF49503. SSF49503. 3 hits.
    PROSITEiPS00079. MULTICOPPER_OXIDASE1. 2 hits.
    PS00080. MULTICOPPER_OXIDASE2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q12729-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFPGARILAT LTLALHLLHG THAAIGPTGD MYIVNEDVSP DGFTRSAVVA    50
    RSDPTTNGTS ETLTGVLVQG NKGDNFQLNV LNQLSDTTML KTTSIHWHGF 100
    FQSGSTWADG PAFVNQCPIA SGNSFLYDFN VPDQAGTFWY HSHLSTQYCD 150
    GLRGPFIVYD PSDPHLSLYD VDNADTIITL EDWYHVVAPQ NAVLPTADST 200
    LINGKGRFAG GPTSALAVIN VESNKRYRFR LISMSCDPNF TFSIDGHSLQ 250
    VIEADAVNIV PIVVDSIQIF AGQRYSFVLN ANQTVDNYWI RADPNLGSTG 300
    FDGGINSAIL RYAGATEDDP TTTSSTSTPL EETNLVPLEN PGAPGPAVPG 350
    GADININLAM AFDVTNFELT INGSPFKAPT APVLLQILSG ATTAASLLPS 400
    GSIYSLEANK VVEISIPALA VGGPHPFHLH GHTFDVIRSA GSTTYNFDTP 450
    ARRDVVNTGT DANDNVTIRF VTDNPGPWFL HCHIDWHLEI GLAVVFAEDV 500
    TSITAPPAAW DDLCPIYDAL SDSDKGGIA 529
    Length:529
    Mass (Da):56,580
    Last modified:November 1, 1996 - v1
    Checksum:i40110AFC4886882F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z34847 mRNA. Translation: CAA84356.1.
    Z22591 Genomic DNA. Translation: CAA80305.1.
    PIRiS49120.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z34847 mRNA. Translation: CAA84356.1 .
    Z22591 Genomic DNA. Translation: CAA80305.1 .
    PIRi S49120.

    3D structure databases

    ProteinModelPortali Q12729.
    SMRi Q12729. Positions 24-525.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.40.420. 3 hits.
    InterProi IPR001117. Cu-oxidase.
    IPR011706. Cu-oxidase_2.
    IPR011707. Cu-oxidase_3.
    IPR002355. Cu_oxidase_Cu_BS.
    IPR008972. Cupredoxin.
    [Graphical view ]
    Pfami PF00394. Cu-oxidase. 1 hit.
    PF07731. Cu-oxidase_2. 1 hit.
    PF07732. Cu-oxidase_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49503. SSF49503. 3 hits.
    PROSITEi PS00079. MULTICOPPER_OXIDASE1. 2 hits.
    PS00080. MULTICOPPER_OXIDASE2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of a laccase gene from the lignin-degrading basidiomycete Pleurotus ostreatus."
      Giardina P., Cannio R., Martirani L., Marzullo L., Palmieri G., Sannia G.
      Appl. Environ. Microbiol. 61:2408-2413(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: Florida.
      Tissue: Mycelium.

    Entry informationi

    Entry nameiLAC1_PLEOS
    AccessioniPrimary (citable) accession number: Q12729
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3