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Protein

Laccase-1

Gene

POX1

Organism
Pleurotus ostreatus (Oyster mushroom) (White-rot fungus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Lignin degradation and detoxification of lignin-derived products.By similarity

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

Cofactori

Cu cationBy similarityNote: Binds 4 Cu cations per monomer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi96 – 961Copper 1; type 2By similarity
Metal bindingi98 – 981Copper 2; type 3By similarity
Metal bindingi141 – 1411Copper 2; type 3By similarity
Metal bindingi143 – 1431Copper 3; type 3By similarity
Metal bindingi425 – 4251Copper 4; type 1By similarity
Metal bindingi428 – 4281Copper 1; type 2By similarity
Metal bindingi430 – 4301Copper 3; type 3By similarity
Metal bindingi481 – 4811Copper 3; type 3By similarity
Metal bindingi482 – 4821Copper 4; type 1By similarity
Metal bindingi483 – 4831Copper 2; type 3By similarity
Metal bindingi487 – 4871Copper 4; type 1By similarity

GO - Molecular functioni

  1. copper ion binding Source: InterPro
  2. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB-EC

GO - Biological processi

  1. lignin catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lignin degradation

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-1 (EC:1.10.3.2)
Alternative name(s):
Benzenediol:oxygen oxidoreductase 1
Diphenol oxidase 1
Urishiol oxidase 1
Gene namesi
Name:POX1
OrganismiPleurotus ostreatus (Oyster mushroom) (White-rot fungus)
Taxonomic identifieri5322 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesPleurotaceaePleurotus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 529506Laccase-1PRO_0000002929Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...)Sequence Analysis
Glycosylationi239 – 2391N-linked (GlcNAc...)Sequence Analysis
Glycosylationi282 – 2821N-linked (GlcNAc...)Sequence Analysis
Glycosylationi465 – 4651N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ12729.
SMRiQ12729. Positions 24-525.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 159135Plastocyanin-like 1Add
BLAST
Domaini170 – 312143Plastocyanin-like 2Add
BLAST
Domaini380 – 499120Plastocyanin-like 3Add
BLAST

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated
Contains 3 plastocyanin-like domains.Curated

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 3 hits.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 2 hits.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12729-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFPGARILAT LTLALHLLHG THAAIGPTGD MYIVNEDVSP DGFTRSAVVA
60 70 80 90 100
RSDPTTNGTS ETLTGVLVQG NKGDNFQLNV LNQLSDTTML KTTSIHWHGF
110 120 130 140 150
FQSGSTWADG PAFVNQCPIA SGNSFLYDFN VPDQAGTFWY HSHLSTQYCD
160 170 180 190 200
GLRGPFIVYD PSDPHLSLYD VDNADTIITL EDWYHVVAPQ NAVLPTADST
210 220 230 240 250
LINGKGRFAG GPTSALAVIN VESNKRYRFR LISMSCDPNF TFSIDGHSLQ
260 270 280 290 300
VIEADAVNIV PIVVDSIQIF AGQRYSFVLN ANQTVDNYWI RADPNLGSTG
310 320 330 340 350
FDGGINSAIL RYAGATEDDP TTTSSTSTPL EETNLVPLEN PGAPGPAVPG
360 370 380 390 400
GADININLAM AFDVTNFELT INGSPFKAPT APVLLQILSG ATTAASLLPS
410 420 430 440 450
GSIYSLEANK VVEISIPALA VGGPHPFHLH GHTFDVIRSA GSTTYNFDTP
460 470 480 490 500
ARRDVVNTGT DANDNVTIRF VTDNPGPWFL HCHIDWHLEI GLAVVFAEDV
510 520
TSITAPPAAW DDLCPIYDAL SDSDKGGIA
Length:529
Mass (Da):56,580
Last modified:November 1, 1996 - v1
Checksum:i40110AFC4886882F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34847 mRNA. Translation: CAA84356.1.
Z22591 Genomic DNA. Translation: CAA80305.1.
PIRiS49120.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34847 mRNA. Translation: CAA84356.1.
Z22591 Genomic DNA. Translation: CAA80305.1.
PIRiS49120.

3D structure databases

ProteinModelPortaliQ12729.
SMRiQ12729. Positions 24-525.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 3 hits.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 2 hits.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequencing of a laccase gene from the lignin-degrading basidiomycete Pleurotus ostreatus."
    Giardina P., Cannio R., Martirani L., Marzullo L., Palmieri G., Sannia G.
    Appl. Environ. Microbiol. 61:2408-2413(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: Florida.
    Tissue: Mycelium.

Entry informationi

Entry nameiLAC1_PLEOS
AccessioniPrimary (citable) accession number: Q12729
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.