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Q12724 (PYRF_YARLI) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Orotidine 5'-phosphate decarboxylase

EC=4.1.1.23
Alternative name(s):
OMP decarboxylase
Short name=OMPDCase
Short name=OMPdecase
Uridine 5'-monophosphate synthase
Short name=UMP synthase
Gene names
Name:URA3
Ordered Locus Names:YALI0E26741g
OrganismYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica) [Reference proteome]
Taxonomic identifier284591 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Orotidine 5'-phosphate = UMP + CO2.

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.

Sequence similarities

Belongs to the OMP decarboxylase family.

Caution

Strain CLIB 122 / E 150 has a defective URA3 sequence (ura3-302) which is disrupted (positions 8 to 249) by S.cerevisiae SUC2.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 286286Orotidine 5'-phosphate decarboxylase
PRO_0000134691

Regions

Region57 – 593Substrate binding By similarity
Region89 – 9810Substrate binding By similarity

Sites

Active site911Proton donor By similarity
Binding site351Substrate By similarity
Binding site2391Substrate By similarity
Binding site2571Substrate By similarity

Experimental info

Sequence conflict104 – 1085KNGVY → RCH in CAC32856. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q12724 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 7E71BFE84C61F216

FASTA28631,630
        10         20         30         40         50         60 
MPSYEARANV HKSAFAARVL KLVAAKKTNL CASLDVTTTK ELIELADKVG PYVCMIKTHI 

        70         80         90        100        110        120 
DIIDDFTYAG TVLPLKELAL KHGFFLFEDR KFADIGNTVK HQYKNGVYRI AEWSDITNAH 

       130        140        150        160        170        180 
GVPGTGIIAG LRAGAEETVS EQKKEDVSDY ENSQYKEFLV PSPNEKLARG LLMLAELSCK 

       190        200        210        220        230        240 
GSLATGEYSK QTIELARSDP EFVVGFIAQN RPKGDSEDWL ILTPGVGLDD KGDALGQQYR 

       250        260        270        280 
TVEDVMSTGT DIIIVGRGLY GQNRDPIEEA KRYQKAGWEA YQKINC 

« Hide

References

« Hide 'large scale' references
[1]"Insertional mutagenesis in the n-alkane-assimilating yeast Yarrowia lipolytica: generation of tagged mutations in genes involved in hydrophobic substrate utilization."
Mauersberger S., Wang H., Gaillardin C., Barth G., Nicaud J.-M.
J. Bacteriol. 183:5102-5109(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 20460 / W29 / CBS 7504 / IFP29.
[2]Strick C.A., James L.C., O'Donnell M.M., Elsenboss L.A.
Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Genome evolution in yeasts."
Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. expand/collapse author list , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-7 AND 250-284.
Strain: CLIB 122 / E 150.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ306421 Genomic DNA. Translation: CAC32856.1.
U40564 Genomic DNA. Translation: AAA85392.1.
CR382131 Genomic DNA. No translation available.

3D structure databases

ProteinModelPortalQ12724.
SMRQ12724. Positions 2-283.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING4952.Q12724.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG0284.
OrthoDBEOG7CVQ76.

Enzyme and pathway databases

UniPathwayUPA00070; UER00120.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR014732. OMPdecase.
IPR018089. OMPdecase_AS.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMSSF51366. SSF51366. 2 hits.
TIGRFAMsTIGR01740. pyrF. 2 hits.
PROSITEPS00156. OMPDECASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRF_YARLI
AccessionPrimary (citable) accession number: Q12724
Secondary accession number(s): Q6C4H6, Q9C1U4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 13, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways