Q12723 (2NPD_CYBMR) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 55.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Nitronate monooxygenase EC=1.13.12.16 Alternative name(s): 2-nitropropane dioxygenase Short name=2-NPD Nitroalkane oxidase |
| Organism | Cyberlindnera mrakii (Yeast) (Williopsis mrakii) |
| Taxonomic identifier | 1004253 [NCBI] |
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Phaffomycetaceae › Cyberlindnera![]() |
Protein attributes
| Sequence length | 374 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Catalyzes the oxidation of alkyl nitronates to produce the corresponding carbonyl compounds and nitrites By similarity. |
| Catalytic activity | Ethylnitronate + O2 = acetaldehyde + nitrite + other products. |
| Cofactor | Binds 1 FMN per subunit. Ref.2 |
| Sequence similarities | Belongs to the nitronate monooxygenase family. |
Ontologies
| Keywords | |
|---|---|
| Ligand | FMN Flavoprotein |
| Molecular function | Monooxygenase Oxidoreductase |
| Gene Ontology (GO) | |
| Molecular_function | nitronate monooxygenase activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 374 | 374 | Nitronate monooxygenase | PRO_0000064361 | |||||
Regions | |||||||||
| Nucleotide binding | 22 – 24 | 3 | FMN By similarity | ||||||
| Nucleotide binding | 238 – 240 | 3 | FMN By similarity | ||||||
| Nucleotide binding | 261 – 262 | 2 | FMN By similarity | ||||||
Sites | |||||||||
| Active site | 197 | 1 | Proton acceptor Potential | ||||||
| Binding site | 197 | 1 | Substrate By similarity | ||||||
Sequences
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References
| [1] | "Unique primary structure of 2-nitropropane dioxygenase from Hansenula mrakii." Tchorzewski M., Kurihara T., Esaki N., Soda K. Eur. J. Biochem. 226:841-846(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NBRC 0895 / NCYC 2251 / VTT C-01434. |
| [2] | "Nitronate monooxygenase, a model for anionic flavin semiquinone intermediates in oxidative catalysis." Gadda G., Francis K. Arch. Biochem. Biophys. 493:53-61(2010) [PubMed] [Europe PMC] [Abstract] Cited for: COFACTOR, SUBSTRATE SPECIFICITY, REACTION MECHANISM. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U13900 Genomic DNA. Translation: AAA64484.1. |
| PIR | S50891. |
3D structure databases | |
| ProteinModelPortal | Q12723. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:MONOMER-12527. |
Family and domain databases | |
| Gene3D | 3.20.20.70. 2 hits. |
| InterPro | IPR004136. 2Npropane_dOase. IPR013785. Aldolase_TIM. [Graphical view] |
| Pfam | PF03060. NMO. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | 2NPD_CYBMR | ||||||||
| Accession | Primary (citable) accession number: Q12723 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

Clusters with
