Reviewed, UniProtKB/Swiss-Prot Q12723 (2NPD_WILMR)

Names and origin

Protein names	Recommended name:     2-nitropropane dioxygenase       Short name=2-NPD     EC=1.13.11.32 Alternative name(s):     Nitroalkane oxidase
Organism	Williopsis mrakii (Yeast) (Hansenula mrakii)
Taxonomic identifier	4963 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Williopsis

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxygenative denitrification of various anionic nitroalkanes.
Catalytic activity	2 2-nitropropane + O2 = 2 acetone + 2 nitrite.
Cofactor	FAD.
Sequence similarities	Belongs to the 2-nitropropane dioxygenase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-nitropropane dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	2-nitropropane dioxygenase		PRO_0000064361

Sequences

	Sequence		Length	Mass (Da)
	Q12723-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 63F82BA7453E43F0	FASTA	374	41,467
	10 20 30 40 50 60 MRSQIQSFLK TFEVRYPIIQ APMAGASTLE LAATVTRLGG IGSIPMGSLS EKCDAIETQL 70 80 90 100 110 120 ENFDELVGDS GRIVNLNFFA HKEPRSGRAD VNEEWLKKYD KIYGKAGIEF DKKELKLLYP 130 140 150 160 170 180 SFRSIVDPQH PTVRLLKNLK PKIVSFHFGL PHEAVIESLQ ASDIKIFVTV TNLQEFQQAY 190 200 210 220 230 240 ESKLDGVVLQ GWEAGGHRGN FKANDVEDGQ LKTLDLVSTI VDYIDSASIS NPPFIIAAGG 250 260 270 280 290 300 IHDDESIKEL LQFNIAAVQL GTVWLPSSQA TISPEHLKMF QSPKSDTMMT AAISGRNLRT 310 320 330 340 350 360 ISTPFLRDLH QSSPLASIPD YPLPYDSFKS LANDAKQSGK GPQYSAFLAG SNYHKSWKDT 370 RSTEEIFSIL VQDL

References

[1]	"Unique primary structure of 2-nitropropane dioxygenase from Hansenula mrakii." Tchorzewski M., Kurihara T., Esaki N., Soda K. Eur. J. Biochem. 226:841-846(1994) [PubMed: 7813473] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IFO 0895.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U13900 Genomic DNA. Translation: AAA64484.1.
PIR	S50891.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-12527.
Family and domain databases
InterPro	IPR004136. 2Npropane_dOase. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF03060. NPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

2NPD_WILMR

Accession

Primary (citable) accession number: Q12723

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	March 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)