Q12723 (2NPD_CYBMR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Nitronate monooxygenase EC=1.13.12.16 Alternative name(s): 2-nitropropane dioxygenase Short name=2-NPD Nitroalkane oxidase
Organism	Cyberlindnera mrakii (Yeast) (Williopsis mrakii)
Taxonomic identifier	1004253 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Phaffomycetaceae › Cyberlindnera

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidation of alkyl nitronates to produce the corresponding carbonyl compounds and nitrites By similarity.
Catalytic activity	Ethylnitronate + O2 = acetaldehyde + nitrite + other products.
Cofactor	Binds 1 FMN per subunit. Ref.2
Sequence similarities	Belongs to the nitronate monooxygenase family.

Ontologies

Keywords
Ligand	FMN Flavoprotein
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Molecular_function	nitronate monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Nitronate monooxygenase		PRO_0000064361
Regions
	Nucleotide binding	22 – 24	3	FMN By similarity
	Nucleotide binding	238 – 240	3	FMN By similarity
	Nucleotide binding	261 – 262	2	FMN By similarity
Sites
	Active site	197	1	Proton acceptor Potential
	Binding site	197	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12723 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 63F82BA7453E43F0	FASTA	374	41,467
	10 20 30 40 50 60 MRSQIQSFLK TFEVRYPIIQ APMAGASTLE LAATVTRLGG IGSIPMGSLS EKCDAIETQL 70 80 90 100 110 120 ENFDELVGDS GRIVNLNFFA HKEPRSGRAD VNEEWLKKYD KIYGKAGIEF DKKELKLLYP 130 140 150 160 170 180 SFRSIVDPQH PTVRLLKNLK PKIVSFHFGL PHEAVIESLQ ASDIKIFVTV TNLQEFQQAY 190 200 210 220 230 240 ESKLDGVVLQ GWEAGGHRGN FKANDVEDGQ LKTLDLVSTI VDYIDSASIS NPPFIIAAGG 250 260 270 280 290 300 IHDDESIKEL LQFNIAAVQL GTVWLPSSQA TISPEHLKMF QSPKSDTMMT AAISGRNLRT 310 320 330 340 350 360 ISTPFLRDLH QSSPLASIPD YPLPYDSFKS LANDAKQSGK GPQYSAFLAG SNYHKSWKDT 370 RSTEEIFSIL VQDL

References

[1]	"Unique primary structure of 2-nitropropane dioxygenase from Hansenula mrakii." Tchorzewski M., Kurihara T., Esaki N., Soda K. Eur. J. Biochem. 226:841-846(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NBRC 0895 / NCYC 2251 / VTT C-01434.
[2]	"Nitronate monooxygenase, a model for anionic flavin semiquinone intermediates in oxidative catalysis." Gadda G., Francis K. Arch. Biochem. Biophys. 493:53-61(2010) [PubMed] [Europe PMC] [Abstract] Cited for: COFACTOR, SUBSTRATE SPECIFICITY, REACTION MECHANISM.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U13900 Genomic DNA. Translation: AAA64484.1.
PIR	S50891.
3D structure databases
ProteinModelPortal	Q12723.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-12527.
Family and domain databases
Gene3D	3.20.20.70. 2 hits.
InterPro	IPR004136. 2Npropane_dOase. IPR013785. Aldolase_TIM. [Graphical view]
Pfam	PF03060. NMO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

2NPD_CYBMR

Accession

Primary (citable) accession number: Q12723

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program