Reviewed, UniProtKB/Swiss-Prot Q12719 (LAC4_TRAVE)

Names and origin

Protein names

Recommended name:
    Laccase-4
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 4
    Urishiol oxidase 4
    Diphenol oxidase 4

Gene names

Name:	LCC4
Synonyms:	LCC1

Organism

Trametes versicolor (White-rot fungus) (Coriolus versicolor)

Taxonomic identifier

5325 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Aphyllophorales › Trametes

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products Probable.
Catalytic activity	4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Secreted
Domain	Repeat Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: InterPro laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 520	498	Laccase-4		PRO_0000002941
Regions
	Domain	24 – 149	126	Plastocyanin-like 1
	Domain	161 – 303	143	Plastocyanin-like 2
	Domain	370 – 491	122	Plastocyanin-like 3
Sites
	Metal binding	86	1	Copper 1; type 2 By similarity
	Metal binding	88	1	Copper 2; type 3 By similarity
	Metal binding	131	1	Copper 2; type 3 By similarity
	Metal binding	133	1	Copper 3; type 3 By similarity
	Metal binding	418	1	Copper 4; type 1 By similarity
	Metal binding	421	1	Copper 1; type 2 By similarity
	Metal binding	423	1	Copper 3; type 3 By similarity
	Metal binding	473	1	Copper 3; type 3 By similarity
	Metal binding	474	1	Copper 4; type 1 By similarity
	Metal binding	475	1	Copper 2; type 3 By similarity
	Metal binding	479	1	Copper 4; type 1 By similarity
Amino acid modifications
	Glycosylation	73	1	N-linked (GlcNAc...) Potential
	Glycosylation	76	1	N-linked (GlcNAc...) Potential
	Glycosylation	239	1	N-linked (GlcNAc...) Potential
	Glycosylation	399	1	N-linked (GlcNAc...) Potential
	Glycosylation	457	1	N-linked (GlcNAc...) Potential
	Glycosylation	497	1	N-linked (GlcNAc...) Potential
	Disulfide bond	107 ↔ 509		Probable
	Disulfide bond	139 ↔ 227		Probable

Sequences

	Sequence		Length	Mass (Da)
	Q12719-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FC1032400E9DDFA4	FASTA	520	55,644
	10 20 30 40 50 60 MGRFSSLCAL TAVIHSFGRV SAAIGPVTDL TISNADVSPD GFTRAAVLAN GVFPGPLITG 70 80 90 100 110 120 NKGDNFQINV IDNLSNETML KSTSIHWHGF FQKGTNWADG AAFVNQCPIA TGNSFLYDFT 130 140 150 160 170 180 ATDQAGTFWY HSHLSTQYCD GLRGPMVVYD PSDPHADLYD VDDETTIITL SDWYHTAASL 190 200 210 220 230 240 GAAFPIGSDS TLINGLGRFA GGDSTDLAVI TVEQGKRYRM RLLSLSCDPN YVFSIDGHNM 250 260 270 280 290 300 TIIEADAVNH EPLTVDSIQI YAGQRYSFVL TADQDIDNYF IRALPSAGTT SFDGGINSAI 310 320 330 340 350 360 LRYSGASEVD PTTTETTSVL PLDEANLVPL DSPAAPGDPN IGGVDYALNL DFNFDGTNFF 370 380 390 400 410 420 INDVSFVSPT VPVLLQILSG TTSAADLLPS GSLFALPSNS TIEISFPITA TNAPGAPHPF 430 440 450 460 470 480 HLHGHTFSIV RTAGSTDTNF VNPVRRDVVN TGTAGDNVTI RFTTDNPGPW FLHCHIDFHL 490 500 510 520 EAGFAIVFSE DTADVSNTTT PSTAWEDLCP TYNALDSSDL

References

[1]

"Characterization of a laccase gene from the white-rot fungus Trametes versicolor and structural features of basidiomycete laccases." Joensson L.J., Sjoestroem K., Haeggstroem I., Nyman P.O. Biochim. Biophys. Acta 1251:210-215(1995) [PubMed: 7669813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PRL 572.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X84683 Genomic DNA. Translation: CAA59161.1.
PIR	S59533.
3D structure databases
HSSP	HSSP built from PDB template 1KYA based on UniProtKB Q96UT7.
SMR	Q12719. Positions 23-519.
ModBase	Search...
Family and domain databases
InterPro	IPR001117. Cu-oxidase. IPR011706. Cu-oxidase_2. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 3 hits.
Pfam	PF00394. Cu-oxidase. 1 hit. PF07731. Cu-oxidase_2. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view]
PROSITE	PS00079. MULTICOPPER_OXIDASE1. 1 hit. PS00080. MULTICOPPER_OXIDASE2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC4_TRAVE

Accession

Primary (citable) accession number: Q12719

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)