Q12708 (PGLR1_SCLSC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endo-polygalacturonase
Short name=PG
EC=3.2.1.15

Alternative name(s):
Pectinase

Gene names

Name:

PG1

Organism

Sclerotinia sclerotiorum (White mold) (Whetzelinia sclerotiorum)

Taxonomic identifier

5180 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Leotiomycetes › Helotiales › Sclerotiniaceae › Sclerotinia

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 6 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Cleavage on pair of basic residues Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 42	25	Potential		PRO_0000024795
	Chain	43 – 380	338	Endo-polygalacturonase		PRO_0000024796
Sites
	Active site	222	1	Proton donor By similarity
	Active site	244	1	By similarity
Amino acid modifications
	Glycosylation	361	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q12708 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 66F7D362A6477B7D	FASTA	380	37,916
	10 20 30 40 50 60 MVHILSSALS LLRLGAAVSA APAPAPTAAP NVADALAAVE KRAGSCTFSD PAVPLPAIKS 70 80 90 100 110 120 KASCATIVIS AVAVPSGTTL DLTGLKSGTH VVFEGTTTFG YEEWSGPLVS VSGTDITVTG 130 140 150 160 170 180 ASGSVLDGNG AKYWDGKGTN GGKTKPKFFY AHSLKGKSSI NNVKILNSPV QVFSINSASG 190 200 210 220 230 240 LTLSGITIDN SAGNSLGHNT DAFDVGSSTD ITISGANVQN QDDCLAINSG TGITFTGGTC 250 260 270 280 290 300 SGGHGLSIGS VGGRSDNVVS DVIIESSTVK NSANGVRIKT VLGATGSVSG VTYKDITLSG 310 320 330 340 350 360 ITSYGVVIEQ DYENGSPTGK PTSGVPITGV TLSNVHGTVS SSATNVYVLC AKCSGWTWDV 370 380 NVTGGKTSTK CAGLPSGVKC

References

[1]	"Cloning and sequence analysis of a polygalacturonase-encoding gene from the phytopathogenic fungus Sclerotinia sclerotiorum." Reymond P., Deleage G., Rascle C., Fevre M. Gene 146:233-237(1994) [PubMed: 8076824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SS5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L12023 Genomic DNA. Translation: AAC14466.1.
3D structure databases
ProteinModelPortal	Q12708.
SMR	Q12708. Positions 45-377.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 6 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR1_SCLSC

Accession

Primary (citable) accession number: Q12708

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 31, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program