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Q12705

- PP2B_SCHPO

UniProt

Q12705 - PP2B_SCHPO

Protein

Serine/threonine-protein phosphatase 2B catalytic subunit

Gene

ppb1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (14 Aug 2001)
      Previous versions | rss
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    Functioni

    Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Appears to be involved in cytokinesis, mating, transport, nuclear and spindle pole body positioning, and cell shape.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 1 Fe3+ ion per subunit.By similarity
    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi119 – 1191IronBy similarity
    Metal bindingi121 – 1211IronBy similarity
    Metal bindingi147 – 1471IronBy similarity
    Metal bindingi147 – 1471ZincBy similarity
    Metal bindingi179 – 1791ZincBy similarity
    Active sitei180 – 1801Proton donorBy similarity
    Metal bindingi228 – 2281ZincBy similarity
    Metal bindingi310 – 3101ZincBy similarity

    GO - Molecular functioni

    1. calcium-dependent protein serine/threonine phosphatase activity Source: PomBase
    2. metal ion binding Source: UniProtKB-KW
    3. phosphatase activity Source: PomBase

    GO - Biological processi

    1. calcium-mediated signaling Source: PomBase
    2. cellular chloride ion homeostasis Source: PomBase
    3. cellular response to salt stress Source: PomBase
    4. cytosolic calcium ion homeostasis Source: PomBase
    5. fungal-type cell wall organization or biogenesis Source: PomBase
    6. negative regulation of calcium ion transport into cytosol Source: PomBase
    7. protein dephosphorylation Source: PomBase
    8. regulation of cell shape Source: UniProtKB-KW
    9. regulation of conjugation with cellular fusion Source: PomBase
    10. regulation of establishment or maintenance of cell polarity regulating cell shape Source: PomBase
    11. regulation of mitotic cytokinesis Source: PomBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell shape

    Keywords - Ligandi

    Calmodulin-binding, Iron, Manganese, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_188228. Ca2+ pathway.
    REACT_205140. FCERI mediated Ca+2 mobilization.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 2B catalytic subunit (EC:3.1.3.16)
    Gene namesi
    Name:ppb1
    ORF Names:SPBC1346.01c, SPBP4H10.04
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBP4H10.04.

    Subcellular locationi

    GO - Cellular componenti

    1. calcineurin complex Source: PomBase
    2. cell division site Source: PomBase
    3. cytoplasm Source: PomBase
    4. cytosol Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 554554Serine/threonine-protein phosphatase 2B catalytic subunitPRO_0000058835Add
    BLAST

    Proteomic databases

    MaxQBiQ12705.

    Interactioni

    Subunit structurei

    Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

    Protein-protein interaction databases

    BioGridi276568. 39 interactions.
    MINTiMINT-4700029.
    STRINGi4896.SPBP4H10.04-1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12705.
    SMRiQ12705. Positions 38-401.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-2B subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    HOGENOMiHOG000172699.
    KOiK04348.
    OMAiRDECECK.
    OrthoDBiEOG77M8X9.
    PhylomeDBiQ12705.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12705-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSGPHNLED PIVRAIRQKN QAPSHDFTIF VQEDGSSVST LDRVVKNVQA    50
    PATYIPTDVE FFDINEPDKP DLHFLRNHFI REGRLSEEQT LYIIKKATEI 100
    LKSEDNLIEV DAPVTVCGDI HGQYYDLMKL FEVGGNPANT QYLFLGDYVD 150
    RGYFSIECLL YLWALKIWYP KTLWLLRGNH ECAHLTDYFT FKLECTHKYN 200
    IKVYEACLQS FNALPLAAIM NKQFLCVHGG LSPELHTLND IRMINRFCEP 250
    PTHGLMCDLL WSDPLEDFGS EKSNKHFIHN NVRGCSYFYS YQAVCTFLEN 300
    NNLLSVIRAH EAQDVGYRMY RKTKTTGFPS LMTIFSAPNY LDVYNNKAAV 350
    LKYENNVMNI RQFNCSPHPY WLPNFMDVFT WSLPFVGEKV SEMLISMLNI 400
    CSKEELYETD LKESAPTQHK QPAPSENENK ADQEIDIEAR RQIIKNKIMA 450
    IGRISRVFSV LREERESVSE LKNVSGTQRL PAGTLMLGAE GIKNAINSFD 500
    DARKLDIQNE RLPPSNSRRR STDLKAFEEV MNSSEDDTSI DHLVERFADK 550
    KSSL 554
    Length:554
    Mass (Da):63,714
    Last modified:August 14, 2001 - v2
    Checksum:iD27ACA21AE2F3E19
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 121I → T in BAA06081. (PubMed:7983142)Curated
    Sequence conflicti129 – 1302KL → EI in BAA06081. (PubMed:7983142)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D28955 Genomic DNA. Translation: BAA06081.1.
    CU329671 Genomic DNA. Translation: CAB83162.1.
    PIRiT45137.
    T50310.
    RefSeqiNP_596178.1. NM_001022097.2.

    Genome annotation databases

    EnsemblFungiiSPBP4H10.04.1; SPBP4H10.04.1:pep; SPBP4H10.04.
    GeneIDi2540024.
    KEGGispo:SPBP4H10.04.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D28955 Genomic DNA. Translation: BAA06081.1 .
    CU329671 Genomic DNA. Translation: CAB83162.1 .
    PIRi T45137.
    T50310.
    RefSeqi NP_596178.1. NM_001022097.2.

    3D structure databases

    ProteinModelPortali Q12705.
    SMRi Q12705. Positions 38-401.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 276568. 39 interactions.
    MINTi MINT-4700029.
    STRINGi 4896.SPBP4H10.04-1.

    Proteomic databases

    MaxQBi Q12705.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBP4H10.04.1 ; SPBP4H10.04.1:pep ; SPBP4H10.04 .
    GeneIDi 2540024.
    KEGGi spo:SPBP4H10.04.

    Organism-specific databases

    PomBasei SPBP4H10.04.

    Phylogenomic databases

    eggNOGi COG0639.
    HOGENOMi HOG000172699.
    KOi K04348.
    OMAi RDECECK.
    OrthoDBi EOG77M8X9.
    PhylomeDBi Q12705.

    Enzyme and pathway databases

    Reactomei REACT_188228. Ca2+ pathway.
    REACT_205140. FCERI mediated Ca+2 mobilization.

    Miscellaneous databases

    NextBioi 20801167.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A calcineurin-like gene ppb1+ in fission yeast: mutant defects in cytokinesis, cell polarity, mating and spindle pole body positioning."
      Yoshida T., Toda T., Yanagida M.
      J. Cell Sci. 107:1725-1735(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.

    Entry informationi

    Entry nameiPP2B_SCHPO
    AccessioniPrimary (citable) accession number: Q12705
    Secondary accession number(s): Q9P7E4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: August 14, 2001
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3