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Q12705

- PP2B_SCHPO

UniProt

Q12705 - PP2B_SCHPO

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Protein

Serine/threonine-protein phosphatase 2B catalytic subunit

Gene

ppb1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Appears to be involved in cytokinesis, mating, transport, nuclear and spindle pole body positioning, and cell shape.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe3+By similarityNote: Binds 1 Fe(3+) ion per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi119 – 1191IronBy similarity
Metal bindingi121 – 1211IronBy similarity
Metal bindingi147 – 1471IronBy similarity
Metal bindingi147 – 1471ZincBy similarity
Metal bindingi179 – 1791ZincBy similarity
Active sitei180 – 1801Proton donorBy similarity
Metal bindingi228 – 2281ZincBy similarity
Metal bindingi310 – 3101ZincBy similarity

GO - Molecular functioni

  1. calcium-dependent protein serine/threonine phosphatase activity Source: PomBase
  2. metal ion binding Source: UniProtKB-KW
  3. phosphatase activity Source: PomBase

GO - Biological processi

  1. calcium-mediated signaling Source: PomBase
  2. cellular chloride ion homeostasis Source: PomBase
  3. cellular response to salt stress Source: PomBase
  4. cytosolic calcium ion homeostasis Source: PomBase
  5. fungal-type cell wall organization or biogenesis Source: PomBase
  6. negative regulation of calcium ion transport into cytosol Source: PomBase
  7. protein dephosphorylation Source: PomBase
  8. regulation of cell shape Source: UniProtKB-KW
  9. regulation of conjugation with cellular fusion Source: PomBase
  10. regulation of mitotic cytokinesis Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell shape

Keywords - Ligandi

Calmodulin-binding, Iron, Manganese, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_188228. Ca2+ pathway.
REACT_258728. DARPP-32 events.
REACT_268922. FCERI mediated Ca+2 mobilization.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit (EC:3.1.3.16)
Gene namesi
Name:ppb1
ORF Names:SPBC1346.01c, SPBP4H10.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBP4H10.04.

Subcellular locationi

GO - Cellular componenti

  1. calcineurin complex Source: PomBase
  2. cell division site Source: PomBase
  3. cytoplasm Source: PomBase
  4. cytosol Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 554554Serine/threonine-protein phosphatase 2B catalytic subunitPRO_0000058835Add
BLAST

Proteomic databases

MaxQBiQ12705.

Interactioni

Subunit structurei

Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

Protein-protein interaction databases

BioGridi276568. 40 interactions.
MINTiMINT-4700029.
STRINGi4896.SPBP4H10.04-1.

Structurei

3D structure databases

ProteinModelPortaliQ12705.
SMRiQ12705. Positions 38-401.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2B subfamily.Curated

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172699.
InParanoidiQ12705.
KOiK04348.
OMAiRDECECK.
OrthoDBiEOG77M8X9.
PhylomeDBiQ12705.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12705-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSGPHNLED PIVRAIRQKN QAPSHDFTIF VQEDGSSVST LDRVVKNVQA
60 70 80 90 100
PATYIPTDVE FFDINEPDKP DLHFLRNHFI REGRLSEEQT LYIIKKATEI
110 120 130 140 150
LKSEDNLIEV DAPVTVCGDI HGQYYDLMKL FEVGGNPANT QYLFLGDYVD
160 170 180 190 200
RGYFSIECLL YLWALKIWYP KTLWLLRGNH ECAHLTDYFT FKLECTHKYN
210 220 230 240 250
IKVYEACLQS FNALPLAAIM NKQFLCVHGG LSPELHTLND IRMINRFCEP
260 270 280 290 300
PTHGLMCDLL WSDPLEDFGS EKSNKHFIHN NVRGCSYFYS YQAVCTFLEN
310 320 330 340 350
NNLLSVIRAH EAQDVGYRMY RKTKTTGFPS LMTIFSAPNY LDVYNNKAAV
360 370 380 390 400
LKYENNVMNI RQFNCSPHPY WLPNFMDVFT WSLPFVGEKV SEMLISMLNI
410 420 430 440 450
CSKEELYETD LKESAPTQHK QPAPSENENK ADQEIDIEAR RQIIKNKIMA
460 470 480 490 500
IGRISRVFSV LREERESVSE LKNVSGTQRL PAGTLMLGAE GIKNAINSFD
510 520 530 540 550
DARKLDIQNE RLPPSNSRRR STDLKAFEEV MNSSEDDTSI DHLVERFADK

KSSL
Length:554
Mass (Da):63,714
Last modified:August 14, 2001 - v2
Checksum:iD27ACA21AE2F3E19
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121I → T in BAA06081. (PubMed:7983142)Curated
Sequence conflicti129 – 1302KL → EI in BAA06081. (PubMed:7983142)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28955 Genomic DNA. Translation: BAA06081.1.
CU329671 Genomic DNA. Translation: CAB83162.1.
PIRiT45137.
T50310.
RefSeqiNP_596178.1. NM_001022097.2.

Genome annotation databases

EnsemblFungiiSPBP4H10.04.1; SPBP4H10.04.1:pep; SPBP4H10.04.
GeneIDi2540024.
KEGGispo:SPBP4H10.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28955 Genomic DNA. Translation: BAA06081.1 .
CU329671 Genomic DNA. Translation: CAB83162.1 .
PIRi T45137.
T50310.
RefSeqi NP_596178.1. NM_001022097.2.

3D structure databases

ProteinModelPortali Q12705.
SMRi Q12705. Positions 38-401.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 276568. 40 interactions.
MINTi MINT-4700029.
STRINGi 4896.SPBP4H10.04-1.

Proteomic databases

MaxQBi Q12705.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBP4H10.04.1 ; SPBP4H10.04.1:pep ; SPBP4H10.04 .
GeneIDi 2540024.
KEGGi spo:SPBP4H10.04.

Organism-specific databases

PomBasei SPBP4H10.04.

Phylogenomic databases

eggNOGi COG0639.
HOGENOMi HOG000172699.
InParanoidi Q12705.
KOi K04348.
OMAi RDECECK.
OrthoDBi EOG77M8X9.
PhylomeDBi Q12705.

Enzyme and pathway databases

Reactomei REACT_188228. Ca2+ pathway.
REACT_258728. DARPP-32 events.
REACT_268922. FCERI mediated Ca+2 mobilization.

Miscellaneous databases

NextBioi 20801167.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A calcineurin-like gene ppb1+ in fission yeast: mutant defects in cytokinesis, cell polarity, mating and spindle pole body positioning."
    Yoshida T., Toda T., Yanagida M.
    J. Cell Sci. 107:1725-1735(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiPP2B_SCHPO
AccessioniPrimary (citable) accession number: Q12705
Secondary accession number(s): Q9P7E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: August 14, 2001
Last modified: November 26, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3