Q12705 (PP2B_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit
EC=3.1.3.16

Gene names

Name:	ppb1
ORF Names:	SPBC1346.01c, SPBP4H10.04

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Appears to be involved in cytokinesis, mating, transport, nuclear and spindle pole body positioning, and cell shape.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 Fe3+ ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Subunit structure	Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-2B subfamily.

Ontologies

Keywords
Biological process	Cell shape
Ligand	Calmodulin-binding Iron Manganese Metal-binding Zinc
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	calcium-mediated signaling Inferred from mutant phenotype PubMed 16928959. Source: PomBase cellular chloride ion homeostasis Inferred from mutant phenotype PubMed 9427748. Source: PomBase cellular response to salt stress Inferred from mutant phenotype PubMed 21811607PubMed 9427748. Source: PomBase cytosolic calcium ion homeostasis Inferred from mutant phenotype PubMed 21811607. Source: PomBase fungal-type cell wall organization or biogenesis Inferred from genetic interaction PubMed 1095095. Source: PomBase negative regulation of calcium ion transport into cytosol Inferred from mutant phenotype PubMed 21811607. Source: PomBase protein dephosphorylation Inferred by curator. Source: PomBase regulation of cell cycle cytokinesis Inferred from mutant phenotype Ref.1. Source: PomBase regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW regulation of conjugation with cellular fusion Inferred from mutant phenotype Ref.1. Source: PomBase regulation of establishment or maintenance of cell polarity regulating cell shape Inferred from mutant phenotype Ref.1. Source: PomBase spindle pole body localization Inferred from mutant phenotype PubMed 1668885. Source: PomBase
Cellular_component	calcineurin complex Inferred from sequence orthology. Source: PomBase cell division site Inferred from direct assay PubMed 16823372. Source: PomBase cytosol Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	calcium-dependent protein serine/threonine phosphatase activity Inferred from mutant phenotype PubMed 16928959. Source: PomBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 554	554	Serine/threonine-protein phosphatase 2B catalytic subunit		PRO_0000058835
Sites
	Active site	180	1	Proton donor By similarity
	Metal binding	119	1	Iron By similarity
	Metal binding	121	1	Iron By similarity
	Metal binding	147	1	Iron By similarity
	Metal binding	147	1	Zinc By similarity
	Metal binding	179	1	Zinc By similarity
	Metal binding	228	1	Zinc By similarity
	Metal binding	310	1	Zinc By similarity
Experimental info
	Sequence conflict	12	1	I → T in BAA06081. Ref.1
	Sequence conflict	129 – 130	2	KL → EI in BAA06081. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q12705 [UniParc]. Last modified August 14, 2001. Version 2. Checksum: D27ACA21AE2F3E19	FASTA	554	63,714
	10 20 30 40 50 60 MTSGPHNLED PIVRAIRQKN QAPSHDFTIF VQEDGSSVST LDRVVKNVQA PATYIPTDVE 70 80 90 100 110 120 FFDINEPDKP DLHFLRNHFI REGRLSEEQT LYIIKKATEI LKSEDNLIEV DAPVTVCGDI 130 140 150 160 170 180 HGQYYDLMKL FEVGGNPANT QYLFLGDYVD RGYFSIECLL YLWALKIWYP KTLWLLRGNH 190 200 210 220 230 240 ECAHLTDYFT FKLECTHKYN IKVYEACLQS FNALPLAAIM NKQFLCVHGG LSPELHTLND 250 260 270 280 290 300 IRMINRFCEP PTHGLMCDLL WSDPLEDFGS EKSNKHFIHN NVRGCSYFYS YQAVCTFLEN 310 320 330 340 350 360 NNLLSVIRAH EAQDVGYRMY RKTKTTGFPS LMTIFSAPNY LDVYNNKAAV LKYENNVMNI 370 380 390 400 410 420 RQFNCSPHPY WLPNFMDVFT WSLPFVGEKV SEMLISMLNI CSKEELYETD LKESAPTQHK 430 440 450 460 470 480 QPAPSENENK ADQEIDIEAR RQIIKNKIMA IGRISRVFSV LREERESVSE LKNVSGTQRL 490 500 510 520 530 540 PAGTLMLGAE GIKNAINSFD DARKLDIQNE RLPPSNSRRR STDLKAFEEV MNSSEDDTSI 550 DHLVERFADK KSSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A calcineurin-like gene ppb1+ in fission yeast: mutant defects in cytokinesis, cell polarity, mating and spindle pole body positioning." Yoshida T., Toda T., Yanagida M. J. Cell Sci. 107:1725-1735(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D28955 Genomic DNA. Translation: BAA06081.1. CU329671 Genomic DNA. Translation: CAB83162.1.
PIR	T45137. T50310.
RefSeq	NP_596178.1. NM_001022097.2.
3D structure databases
ProteinModelPortal	Q12705.
SMR	Q12705. Positions 38-401.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPBP4H10.04-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBP4H10.04.1; SPBP4H10.04.1:pep; SPBP4H10.04.
GeneID	2540024.
KEGG	spo:SPBP4H10.04.
Organism-specific databases
PomBase	SPBP4H10.04.
Phylogenomic databases
eggNOG	COG0639.
HOGENOM	HOG000172699.
KO	K04348.
OMA	FEDETHT.
OrthoDB	EOG45XC4G.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20801167.

Entry information

Entry name

PP2B_SCHPO

Accession

Primary (citable) accession number: Q12705
Secondary accession number(s): Q9P7E4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	August 14, 2001
Last modified:	May 1, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program