Q12704 (DPOG_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase gamma
EC=2.7.7.7

Alternative name(s):
Mitochondrial DNA polymerase catalytic subunit

Gene names

Name:	mip1
ORF Names:	SPCC24B10.22, SPCPB16A4.01

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	1018 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the replication of mitochondrial DNA.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Cofactor	Magnesium.
Subcellular location	Mitochondrion.
Miscellaneous	In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.
Sequence similarities	Belongs to the DNA polymerase type-A family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Mitochondrion
Ligand	DNA-binding Magnesium
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial DNA replication Inferred from sequence orthology. Source: PomBase
Cellular_component	gamma DNA polymerase complex Inferred from electronic annotation. Source: InterPro mitochondrion Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	DNA binding Inferred from sequence or structural similarity. Source: PomBase DNA-directed DNA polymerase activity Inferred from sequence orthology. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1018	1018	DNA polymerase gamma		PRO_0000101277
Experimental info
	Sequence conflict	563	1	G → C in CAA88012. Ref.1
	Sequence conflict	571 – 572	2	QR → HA in CAA88012. Ref.1
	Sequence conflict	832	1	T → Q in CAA88012. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q12704 [UniParc]. Last modified March 5, 2002. Version 2. Checksum: 9B114BC6FBEE63CA	FASTA	1,018	116,045
	10 20 30 40 50 60 MFYKACPSTL TCSKWIHSII KTKKFLYCRH YSSKSFIDNA PLRINPVGVQ YLSPALQNQV 70 80 90 100 110 120 FPQQNTQISQ LHLDLAKFHL AKHQLLNKET IKLPSFNFRL PPLQGKTISE HFYNIGLEFA 130 140 150 160 170 180 EPHLSKAIKF SKIDTPVQPK TWKRQPGWTK YAKDGSISCV PYPDSDCMVF DVEVLYKVSP 190 200 210 220 230 240 FAVVATAVSE DAWYCWLSPW LLGKSENDRQ LIPSNPKGAL FVGHNVSFDR QRIREEYNIK 250 260 270 280 290 300 SSRNVFLDTM SLHVATHGMC SRQKPTWFKA RKAYIRSQST ETSEDDDSSS FDDDYQNYLK 310 320 330 340 350 360 QEPWLAHSSV NSLKDVAKFH CNITLDKSKR DDFASLEKEP ILQKLNELIT YCAHDTYSTH 370 380 390 400 410 420 QVFKKVFPQF LEVCPHPATF SAMLSLGSVF LPVNHSWTRY INGVEEQYQQ MIQLVDQKLS 430 440 450 460 470 480 QYAEKAKDLI NTKDTVLKDP WLRQLDWTPC NLYRKLKKAT QEVPVVPKWY KKAYCKTEKR 490 500 510 520 530 540 AVITAKSRLA PILLRLKWKK HPLAWSDTYG WVFSVERTSK DEIEMLLDQG LVPCSREEDT 550 560 570 580 590 600 KLDYNNYIFF KVPHKDGPEA RCGSPLSKSY QRYFEEGILQ SDYEVAKKAL EMSASCSYWS 610 620 630 640 650 660 SARDRIRSQM VVWDKDAELG VPSSVDGFGI ILPCIIPMGT VTRRAVENTW LTASNSKKNR 670 680 690 700 710 720 LGSELKAMIR APDGYTFVGA DVDSEELWIV ALMGDSQFRL HGATALGMMT LEGKKSEGTD 730 740 750 760 770 780 LHSKTAAILG VSRDSAKVFN YGRLYGAGLK HTTLLLMQMN PTLKTAEAKE LAKKLYASTK 790 800 810 820 830 840 GVKSKMSKRL QEMGLPKLTF WSQGTESFVF NKLEAMAQLP SPRTPVLDAG ITQALSSKNL 850 860 870 880 890 900 SKNSFMTSRV NWAIQSSAVD YLHLLLVSMN HLIKKYYLEA RLSLTVHDEV RYLSSDKDKY 910 920 930 940 950 960 RVAFALQVAN LWTRAFFCQR LGINELPQSV AFFSSVDIDH VLRKDVKMDC VTPSNKVPIP 970 980 990 1000 1010 PGEELTIESV LEKLEQSGQS LEPLEQIQCF VDVKATTSAE ITEEDKKNIA YLKAQAFY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a new DNA polymerase from Schizosaccharomyces pombe: a probable homologue of the Saccharomyces cerevisiae DNA polymerase gamma." Ropp P.A., Copeland W.C. Gene 165:103-107(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SP808.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z47976 Genomic DNA. Translation: CAA88012.1. CU329672 Genomic DNA. Translation: CAB76231.1.
PIR	JC4375. T50429.
RefSeq	NP_588025.2. NM_001023016.2.
3D structure databases
ProteinModelPortal	Q12704.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPCC24B10.22-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPCC24B10.22.1; SPCC24B10.22.1:pep; SPCC24B10.22.
GeneID	2539099.
KEGG	spo:SPCC24B10.22.
Organism-specific databases
PomBase	SPCC24B10.22.
Phylogenomic databases
eggNOG	COG0749.
HOGENOM	HOG000176668.
KO	K02332.
OMA	DIPGCWF.
OrthoDB	EOG4CZFQ1.
Gene expression databases
GermOnline	SPCC24B10.22. Schizosaccharomyces pombe.
Family and domain databases
InterPro	IPR002297. DNA-dir_DNA_pol_A_mt. IPR001098. DNA-dir_DNA_pol_A_palm_dom. IPR012337. RNaseH-like_dom. [Graphical view]
PANTHER	PTHR10267. PTHR10267. 1 hit.
Pfam	PF00476. DNA_pol_A. 1 hit. [Graphical view]
PRINTS	PR00867. DNAPOLG.
SMART	SM00482. POLAc. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
PROSITE	PS00447. DNA_POLYMERASE_A. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	20800271.

Entry information

Entry name

DPOG_SCHPO

Accession

Primary (citable) accession number: Q12704
Secondary accession number(s): Q96WV3, Q9P7I4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	March 5, 2002
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program