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Protein

Serine/threonine-protein kinase ksg1

Gene

ksg1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the control of sexual development and cell growth under stressed conditions. Phosphorylates AGC kinase gad8 at 'Thr-387', activating gad8 kinase activity and promoting sexual development (PubMed:12805221). Phosphorylates AGC kinase psk1 at 'Ser-248', activating psk1 kinase activity and promoting phosphorylation of ribosomal protein S6 (PubMed:22976295).3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei128 – 1281ATPBy similarity
Binding sitei184 – 1841ATPBy similarity
Active sitei223 – 2231Proton acceptorPROSITE-ProRule annotation
Binding sitei227 – 2271ATP; via carbonyl oxygenBy similarity
Binding sitei241 – 2411ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi109 – 1113ATPBy similarity
Nucleotide bindingi178 – 1803ATPBy similarity

GO - Molecular functioni

  • 3-phosphoinositide-dependent protein kinase activity Source: PomBase
  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: PomBase
  • protein serine/threonine kinase activity Source: PomBase

GO - Biological processi

  • fungal-type cell wall organization or biogenesis Source: PomBase
  • peptidyl-serine phosphorylation Source: PomBase
  • peptidyl-threonine phosphorylation Source: PomBase
  • protein autophosphorylation Source: PomBase
  • signal transduction Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 5613.
ReactomeiR-SPO-114604. GPVI-mediated activation cascade.
R-SPO-1257604. PIP3 activates AKT signaling.
R-SPO-165158. Activation of AKT2.
R-SPO-202424. Downstream TCR signaling.
R-SPO-2730905. Role of LAT2/NTAL/LAB on calcium mobilization.
R-SPO-389357. CD28 dependent PI3K/Akt signaling.
R-SPO-389513. CTLA4 inhibitory signaling.
R-SPO-392451. G beta:gamma signalling through PI3Kgamma.
R-SPO-5218920. VEGFR2 mediated vascular permeability.
R-SPO-5218921. VEGFR2 mediated cell proliferation.
R-SPO-5625740. RHO GTPases activate PKNs.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase ksg11 Publication (EC:2.7.11.12 Publications)
Alternative name(s):
PDK1-like kinase ksg11 Publication
Gene namesi
Name:ksg11 Publication
ORF Names:SPCC576.15cImported
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC576.15c.
PomBaseiSPCC576.15c. ksg1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 592592Serine/threonine-protein kinase ksg1PRO_0000086226Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei64 – 641Phosphoserine1 Publication
Modified residuei69 – 691Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12701.

PTM databases

iPTMnetiQ12701.

Interactioni

Protein-protein interaction databases

BioGridi276079. 12 interactions.
MINTiMINT-4699978.

Structurei

3D structure databases

ProteinModelPortaliQ12701.
SMRiQ12701. Positions 91-383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini99 – 366268Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini461 – 572112PHAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni130 – 17546PIF-pocketBy similarityAdd
BLAST

Domaini

The PIF-pocket is a small lobe in the catalytic domain required by the enzyme for the binding to the hydrophobic motif of its substrates. It is an allosteric regulatory site that can accommodate small compounds acting as allosteric inhibitors.By similarity

Sequence similaritiesi

Contains 1 PH domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000233026.
InParanoidiQ12701.
KOiK06276.
OMAiGINGNDA.
OrthoDBiEOG76MKKG.
PhylomeDBiQ12701.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12701-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNTHNPNET EASEDAENDT QSESDLSFDH GSSEKLNRAS LPKTQNSAIP
60 70 80 90 100
QSNALNTTPN ESTSQIDSSP KIPSAVPHIS TPNPSSGAST PNIKRVSDFK
110 120 130 140 150
FGEILGEGSY STVLTATENS TKREYAIKVL DKRHIIKEKK EKYVNIEKEA
160 170 180 190 200
LCILSKHPGF IKLFYTFQDA HNLYFVLSLA RNGELLDYIN KLGRFNEICA
210 220 230 240 250
QYYAALIVDS IDYMHGRGVI HRDLKPENIL LDDNMRTKIT DFGSAKILNS
260 270 280 290 300
SHGSHEEDTH HADKPQAHSR SFVGTARYVS PEVLSDKIAG TASDIWAFGC
310 320 330 340 350
ILFQMLAGKP PFVAGNEYLT FQSILHLSYE IPPDISDVAS DLIKKLLVLD
360 370 380 390 400
PKDRLTVDEI HQHPFFNGIK FDNTLWELPP PRLKPFGHTS VLSLSVPNAS
410 420 430 440 450
NKHENGDLTS PLGVPSMVSA STNAAPSPVG TFNRGTLLPC QSNLEEENKE
460 470 480 490 500
WSSILQDDEK ISKIGTLNVY SMSGINGNDA FRFFSSLFRK RKPRTFILTN
510 520 530 540 550
FGRYLCVASD GEGRKTVKEE IPIKSVGMRC RMVKNNEHGW VVETPTKSWS
560 570 580 590
FEDPNGPASA WVELLDKASS ISLPFGNHSV TSFSRSIARS AV
Length:592
Mass (Da):65,662
Last modified:November 1, 1998 - v2
Checksum:iB9A857D1989F2C61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99280 Genomic DNA. Translation: CAA67672.1.
CU329672 Genomic DNA. Translation: CAA21194.1.
PIRiT43402.
RefSeqiNP_588442.1. NM_001023433.2.

Genome annotation databases

EnsemblFungiiSPCC576.15c.1; SPCC576.15c.1:pep; SPCC576.15c.
GeneIDi2539517.
KEGGispo:SPCC576.15c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99280 Genomic DNA. Translation: CAA67672.1.
CU329672 Genomic DNA. Translation: CAA21194.1.
PIRiT43402.
RefSeqiNP_588442.1. NM_001023433.2.

3D structure databases

ProteinModelPortaliQ12701.
SMRiQ12701. Positions 91-383.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276079. 12 interactions.
MINTiMINT-4699978.

PTM databases

iPTMnetiQ12701.

Proteomic databases

MaxQBiQ12701.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC576.15c.1; SPCC576.15c.1:pep; SPCC576.15c.
GeneIDi2539517.
KEGGispo:SPCC576.15c.

Organism-specific databases

EuPathDBiFungiDB:SPCC576.15c.
PomBaseiSPCC576.15c. ksg1.

Phylogenomic databases

HOGENOMiHOG000233026.
InParanoidiQ12701.
KOiK06276.
OMAiGINGNDA.
OrthoDBiEOG76MKKG.
PhylomeDBiQ12701.

Enzyme and pathway databases

BRENDAi2.7.11.1. 5613.
ReactomeiR-SPO-114604. GPVI-mediated activation cascade.
R-SPO-1257604. PIP3 activates AKT signaling.
R-SPO-165158. Activation of AKT2.
R-SPO-202424. Downstream TCR signaling.
R-SPO-2730905. Role of LAT2/NTAL/LAB on calcium mobilization.
R-SPO-389357. CD28 dependent PI3K/Akt signaling.
R-SPO-389513. CTLA4 inhibitory signaling.
R-SPO-392451. G beta:gamma signalling through PI3Kgamma.
R-SPO-5218920. VEGFR2 mediated vascular permeability.
R-SPO-5218921. VEGFR2 mediated cell proliferation.
R-SPO-5625740. RHO GTPases activate PKNs.

Miscellaneous databases

NextBioi20800678.
PROiQ12701.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A Schizosaccharomyces pombe gene, ksg1, that shows structural homology to the human phosphoinositide-dependent protein kinase PDK1, is essential for growth, mating and sporulation."
    Niederberger C., Schweingruber M.E.
    Mol. Gen. Genet. 261:177-183(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: ATCC 38364 / 968.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Schizosaccharomyces pombe AGC family kinase Gad8p forms a conserved signaling module with TOR and PDK1-like kinases."
    Matsuo T., Kubo Y., Watanabe Y., Yamamoto M.
    EMBO J. 22:3073-3083(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
  4. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64 AND SER-69, IDENTIFICATION BY MASS SPECTROMETRY.
  6. "Psk1, an AGC kinase family member in fission yeast, is directly phosphorylated and controlled by TORC1 and functions as S6 kinase."
    Nakashima A., Otsubo Y., Yamashita A., Sato T., Yamamoto M., Tamanoi F.
    J. Cell Sci. 125:5840-5849(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiKSG1_SCHPO
AccessioniPrimary (citable) accession number: Q12701
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: November 1, 1998
Last modified: May 11, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.