Reviewed, UniProtKB/Swiss-Prot Q126P7 (T23O_POLSJ)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase
      Short name=TDO
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase
      Short name=Tryptophanase
    Tryptophan oxygenase
      Short name=TRPO
      Short name=TO
    Tryptamin 2,3-dioxygenase

Gene names

Name:	kynA
Ordered Locus Names:	Bpro_3590

Organism

Polaromonas sp. (strain JS666 / ATCC BAA-500) [Complete proteome] [HAMAP]

Taxonomic identifier

296591 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Polaromonas

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Tryptophan 2,3-dioxygenase		PRO_0000360122
Regions
	Region	38 – 42	5	Substrate binding By similarity
	Region	63 – 67	5	Substrate binding By similarity
Sites
	Metal binding	252	1	Iron (heme axial ligand) By similarity
	Binding site	125	1	Substrate By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	136	1	Heme By similarity
	Binding site	266	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q126P7-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: EBDCD547F2BB5BF8	FASTA	294	33,527
	10 20 30 40 50 60 MCPHAHPPAK PDHGATTTPE RIVHDEKAQL DFSRDMSYGD YLQLDAILNA QKPLSPAHDE 70 80 90 100 110 120 MLFIIQHQTS ELWMKLMLHE LRAAIANVAA DELGSAFKML ARVSRIMEQL VHAWDVLATM 130 140 150 160 170 180 TPPEYSAMRP YLGASSGFQS YQYRCIEFAL GNKNAAMLQP HAHRSDLLAQ VQAAYEAPSL 190 200 210 220 230 240 YDEALRLLAR RGLAVPASHT ERDWTQPYLE SAAVEQAWLT VYRDPKQYWD LYQLGEELTD 250 260 270 280 290 LEDAFRLWRF RHVTTVERII GFKPGTGGTS GVSYLRKMLD VVLFPEIWKL RTDL

References

[1]

"Complete sequence of chromosome of Polaromonas sp. JS666." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Munk A.C., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000316 Genomic DNA. Translation: ABE45495.1.
RefSeq	YP_550393.1.
3D structure databases
SMR	Q126P7. Positions 34-294.
ModBase	Search...
Protein-protein interaction databases
STRING	Q126P7.
Genome annotation databases
GeneID	4013736.
GenomeReviews	Gene locus Bpro_3590 in contig CP000316_GR.
KEGG	pol:Bpro_3590.
NMPDR	fig|296591.1.peg.5182.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q126P7.
OMA	QWSVLAT.
Enzyme and pathway databases
BioCyc	PSP296591:BPRO_3590-MON.
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03036. trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_POLSJ

Accession

Primary (citable) accession number: Q126P7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	August 22, 2006
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)