Reviewed, UniProtKB/Swiss-Prot Q126F5 (ASPD_POLSJ)

Names and origin

Protein names

Recommended name:
    Probable L-aspartate dehydrogenase
    EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	Bpro_3686

Organism

Polaromonas sp. (strain JS666 / ATCC BAA-500) [Complete proteome] [HAMAP]

Taxonomic identifier

296591 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Polaromonas

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity. HAMAP MF_01265
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity. HAMAP MF_01265
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP NADP catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: HAMAP NADP or NADPH binding Inferred from electronic annotation. Source: HAMAP aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Probable L-aspartate dehydrogenase HAMAP MF_01265		PRO_1000067310
Sites
	Active site	220	1	By similarity
	Binding site	124	1	NAD; via amide nitrogen By similarity
	Binding site	190	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q126F5-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: F1000ACBA87EB262	FASTA	267	27,568
	10 20 30 40 50 60 MLKIAMIGCG AIGASVLELL HGDSDVVVDR VITVPEARDR TEIAVARWAP RARVLEVLAA 70 80 90 100 110 120 DDAPDLVVEC AGHGAIAAHV VPALERGIPC VVTSVGALSA PGMAQLLEQA ARRGKTQVQL 130 140 150 160 170 180 LSGAIGGIDA LAAARVGGLD SVVYTGRKPP MAWKGTPAEA VCDLDSLTVA HCIFDGSAEQ 190 200 210 220 230 240 AAQLYPKNAN VAATLSLAGL GLKRTQVQLF ADPGVSENVH HVAAHGAFGS FELTMRGRPL 250 260 AANPKTSALT VYSVVRALLN RGRALVI

References

[1]

"Complete sequence of chromosome of Polaromonas sp. JS666." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Munk A.C., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000316 Genomic DNA. Translation: ABE45587.1.
RefSeq	YP_550485.1.
3D structure databases
SMR	Q126F5. Positions 2-267.
ModBase	Search...
Protein-protein interaction databases
STRING	Q126F5.
Genome annotation databases
GeneID	4013636.
GenomeReviews	Gene locus Bpro_3686 in contig CP000316_GR.
KEGG	pol:Bpro_3686.
NMPDR	fig|296591.1.peg.1652.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1712.
HOGENOM	HBG649642.
OMA	ECAGHSA.
Enzyme and pathway databases
BioCyc	PSP296591:BPRO_3686-MONOMER.
Family and domain databases
HAMAP	MF_01265. NadX. [Tree]
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. IPR020626. Asp_DH_NAD_syn_prok. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASPD_POLSJ

Accession

Primary (citable) accession number: Q126F5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 22, 2006
Last modified:	January 19, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)