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Protein

Amidophosphoribosyltransferase

Gene

ADE4

Organism
Lachancea kluyveri (Yeast) (Saccharomyces kluyveri)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per subunit.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (ADE4)
  2. no protein annotated in this organism
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2NucleophilePROSITE-ProRule annotation1
Metal bindingi373MagnesiumBy similarity1
Metal bindingi374MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Gene namesi
Name:ADE4
OrganismiLachancea kluyveri (Yeast) (Saccharomyces kluyveri)
Taxonomic identifieri4934 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeLachancea

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001396452 – 510AmidophosphoribosyltransferaseAdd BLAST509

Structurei

3D structure databases

ProteinModelPortaliQ12698.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 239Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST238

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF. 1 hit.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12698-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGILGIALA DQSSVVAPEL FDGSLFLQHR GQDAAGMATC GERGRLYQCK
60 70 80 90 100
GNGMARDVFT QHRMSGLVGS MGIAHLRYPT AGSCANSEAQ PFYVNSPYGI
110 120 130 140 150
CLSHNGTLVN TLSLRSYLDE VVHRHINTDS DSELLLNVFA AELERHNKYR
160 170 180 190 200
VNNDDIFHAL EGVYRQCRGG YACVGMLAGY SLFGFRDPNG IRPLLFGERV
210 220 230 240 250
NPDGTKDYML ASESVVLKAH NFNKFRDLKP GEAVIIPKDC NKQEPEFRQV
260 270 280 290 300
VPTNSYRPDL FEYVYFARPD SVLDGISVYH TRLQMGIKLA ENVKKVVDPD
310 320 330 340 350
EIDVVVSVPD TARTCALQCA NHLNKPYREA FVKNRYVGRT FIMPNQKERV
360 370 380 390 400
SSVRRKLNPM DFEFKDKRVL IVDDSIVRGT TSKEIINMAK ESGATKVYFA
410 420 430 440 450
SAAPAIRFNH IYGIDLADTK QLVAYNRTTD EVAQELGCEK VIYQSLEDLV
460 470 480 490 500
DCCKTDKIDK FEVGVFTGNY VTGVEDGYLQ ELERVRALNK AKLTTAKAEV
510
DIGLYNSADY
Length:510
Mass (Da):57,012
Last modified:November 1, 1996 - v1
Checksum:iC3B3580B3F460378
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32992 Genomic DNA. Translation: AAA75450.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32992 Genomic DNA. Translation: AAA75450.1.

3D structure databases

ProteinModelPortaliQ12698.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC44.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF. 1 hit.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUR1_LACKL
AccessioniPrimary (citable) accession number: Q12698
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.