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Protein

Amidophosphoribosyltransferase

Gene

ADE4

Organism
Lachancea kluyveri (Yeast) (Saccharomyces kluyveri)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per subunit.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (ADE4)
  2. no protein annotated in this organism
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2NucleophilePROSITE-ProRule annotation1
Metal bindingi373MagnesiumBy similarity1
Metal bindingi374MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124

Protein family/group databases

MEROPSiC44.001

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Gene namesi
Name:ADE4
OrganismiLachancea kluyveri (Yeast) (Saccharomyces kluyveri)
Taxonomic identifieri4934 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeLachancea

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001396452 – 510AmidophosphoribosyltransferaseAdd BLAST509

Structurei

3D structure databases

ProteinModelPortaliQ12698
SMRiQ12698
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 239Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST238

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

CDDicd00715 GPATase_N, 1 hit
cd06223 PRTases_typeI, 1 hit
Gene3Di3.60.20.10, 2 hits
HAMAPiMF_01931 PurF, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
IPR005854 PurF
IPR035584 PurF_N
PfamiView protein in Pfam
PF00156 Pribosyltran, 1 hit
PIRSFiPIRSF000485 Amd_phspho_trans, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
SSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01134 purF, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
PS00103 PUR_PYR_PR_TRANSFER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12698-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGILGIALA DQSSVVAPEL FDGSLFLQHR GQDAAGMATC GERGRLYQCK
60 70 80 90 100
GNGMARDVFT QHRMSGLVGS MGIAHLRYPT AGSCANSEAQ PFYVNSPYGI
110 120 130 140 150
CLSHNGTLVN TLSLRSYLDE VVHRHINTDS DSELLLNVFA AELERHNKYR
160 170 180 190 200
VNNDDIFHAL EGVYRQCRGG YACVGMLAGY SLFGFRDPNG IRPLLFGERV
210 220 230 240 250
NPDGTKDYML ASESVVLKAH NFNKFRDLKP GEAVIIPKDC NKQEPEFRQV
260 270 280 290 300
VPTNSYRPDL FEYVYFARPD SVLDGISVYH TRLQMGIKLA ENVKKVVDPD
310 320 330 340 350
EIDVVVSVPD TARTCALQCA NHLNKPYREA FVKNRYVGRT FIMPNQKERV
360 370 380 390 400
SSVRRKLNPM DFEFKDKRVL IVDDSIVRGT TSKEIINMAK ESGATKVYFA
410 420 430 440 450
SAAPAIRFNH IYGIDLADTK QLVAYNRTTD EVAQELGCEK VIYQSLEDLV
460 470 480 490 500
DCCKTDKIDK FEVGVFTGNY VTGVEDGYLQ ELERVRALNK AKLTTAKAEV
510
DIGLYNSADY
Length:510
Mass (Da):57,012
Last modified:November 1, 1996 - v1
Checksum:iC3B3580B3F460378
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32992 Genomic DNA Translation: AAA75450.1

Similar proteinsi

Entry informationi

Entry nameiPUR1_LACKL
AccessioniPrimary (citable) accession number: Q12698
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: February 28, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health