Q12698 (PUR1_LACKL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Amidophosphoribosyltransferase
Short name=ATase
EC=2.4.2.14

Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase

Gene names

Name:

ADE4

Organism

Lachancea kluyveri (Yeast) (Saccharomyces kluyveri)

Taxonomic identifier

4934 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Lachancea

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.
Cofactor	Binds 1 magnesium ion per subunit.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.
Sequence similarities	In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	Magnesium Metal-binding
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW nucleoside metabolic process Inferred from electronic annotation. Source: InterPro purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	amidophosphoribosyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Amidophosphoribosyltransferase		PRO_0000139645
Regions
	Domain	2 – 239	238	Glutamine amidotransferase type-2
Sites
	Active site	2	1	For GATase activity By similarity
	Metal binding	373	1	Magnesium By similarity
	Metal binding	374	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12698 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C3B3580B3F460378	FASTA	510	57,012
	10 20 30 40 50 60 MCGILGIALA DQSSVVAPEL FDGSLFLQHR GQDAAGMATC GERGRLYQCK GNGMARDVFT 70 80 90 100 110 120 QHRMSGLVGS MGIAHLRYPT AGSCANSEAQ PFYVNSPYGI CLSHNGTLVN TLSLRSYLDE 130 140 150 160 170 180 VVHRHINTDS DSELLLNVFA AELERHNKYR VNNDDIFHAL EGVYRQCRGG YACVGMLAGY 190 200 210 220 230 240 SLFGFRDPNG IRPLLFGERV NPDGTKDYML ASESVVLKAH NFNKFRDLKP GEAVIIPKDC 250 260 270 280 290 300 NKQEPEFRQV VPTNSYRPDL FEYVYFARPD SVLDGISVYH TRLQMGIKLA ENVKKVVDPD 310 320 330 340 350 360 EIDVVVSVPD TARTCALQCA NHLNKPYREA FVKNRYVGRT FIMPNQKERV SSVRRKLNPM 370 380 390 400 410 420 DFEFKDKRVL IVDDSIVRGT TSKEIINMAK ESGATKVYFA SAAPAIRFNH IYGIDLADTK 430 440 450 460 470 480 QLVAYNRTTD EVAQELGCEK VIYQSLEDLV DCCKTDKIDK FEVGVFTGNY VTGVEDGYLQ 490 500 510 ELERVRALNK AKLTTAKAEV DIGLYNSADY

References

[1]	Marsh L., Branda S. Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IH404.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U32992 Genomic DNA. Translation: AAA75450.1.
3D structure databases
ProteinModelPortal	Q12698.
SMR	Q12698. Positions 2-485.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00074; UER00124.
Family and domain databases
InterPro	IPR005854. Amd_phspho_trans. IPR017932. GATase_2_dom. IPR000583. GATase_dom. IPR000836. PRibTrfase_dom. [Graphical view]
Pfam	PF00310. GATase_2. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
PIRSF	PIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMs	TIGR01134. purF. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR1_LACKL

Accession

Primary (citable) accession number: Q12698

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	October 31, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program