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Protein

Vacuolar cation-transporting ATPase YPK9

Gene

YPK9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Vacuolar transporter which plays a role in sequestration of divalent heavy metal ions.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei7814-aspartylphosphate intermediateBy similarity1
Metal bindingi1187MagnesiumBy similarity1
Metal bindingi1191MagnesiumBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cation-transporting ATPase activity Source: GO_Central
  • cupric ion binding Source: ParkinsonsUK-UCL
  • manganese ion binding Source: ParkinsonsUK-UCL
  • zinc ion binding Source: ParkinsonsUK-UCL

GO - Biological processi

  • cellular calcium ion homeostasis Source: GO_Central
  • cellular manganese ion homeostasis Source: ParkinsonsUK-UCL
  • cellular zinc ion homeostasis Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33776-MONOMER.
ReactomeiR-SCE-936837. Ion transport by P-type ATPases.

Protein family/group databases

TCDBi3.A.3.10.8. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar cation-transporting ATPase YPK9 (EC:3.6.3.-)
Alternative name(s):
PARK9 homolog
Gene namesi
Name:YPK9
Ordered Locus Names:YOR291W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR291W.
SGDiS000005817. YPK9.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 293CytoplasmicSequence analysisAdd BLAST293
Transmembranei294 – 315HelicalSequence analysisAdd BLAST22
Topological domaini316 – 321VacuolarSequence analysis6
Transmembranei322 – 344HelicalSequence analysisAdd BLAST23
Topological domaini345 – 488CytoplasmicSequence analysisAdd BLAST144
Transmembranei489 – 511HelicalSequence analysisAdd BLAST23
Topological domaini512 – 514VacuolarSequence analysis3
Transmembranei515 – 533HelicalSequence analysisAdd BLAST19
Topological domaini534 – 693CytoplasmicSequence analysisAdd BLAST160
Transmembranei694 – 713HelicalSequence analysisAdd BLAST20
Topological domaini714 – 726VacuolarSequence analysisAdd BLAST13
Transmembranei727 – 748HelicalSequence analysisAdd BLAST22
Topological domaini749 – 1244CytoplasmicSequence analysisAdd BLAST496
Transmembranei1245 – 1264HelicalSequence analysisAdd BLAST20
Topological domaini1265 – 1271VacuolarSequence analysis7
Transmembranei1272 – 1289HelicalSequence analysisAdd BLAST18
Topological domaini1290 – 1307CytoplasmicSequence analysisAdd BLAST18
Transmembranei1308 – 1331HelicalSequence analysisAdd BLAST24
Topological domaini1332 – 1351VacuolarSequence analysisAdd BLAST20
Transmembranei1352 – 1374HelicalSequence analysisAdd BLAST23
Topological domaini1375 – 1387CytoplasmicSequence analysisAdd BLAST13
Transmembranei1388 – 1407HelicalSequence analysisAdd BLAST20
Topological domaini1408 – 1423VacuolarSequence analysisAdd BLAST16
Transmembranei1424 – 1446HelicalSequence analysisAdd BLAST23
Topological domaini1447 – 1472CytoplasmicSequence analysisAdd BLAST26

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • integral component of plasma membrane Source: GO_Central
  • vacuole-mitochondrion membrane contact site Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Disruption phenotypei

Confers sensitivity for growth for cadmium, manganese, nickel or selenium.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000463511 – 1472Vacuolar cation-transporting ATPase YPK9Add BLAST1472

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei95PhosphothreonineCombined sources1
Modified residuei108PhosphoserineCombined sources1
Modified residuei1117PhosphoserineCombined sources1
Modified residuei1120PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ12697.
PRIDEiQ12697.

PTM databases

iPTMnetiQ12697.

Interactioni

Protein-protein interaction databases

BioGridi34679. 76 interactors.
MINTiMINT-4503690.

Structurei

3D structure databases

ProteinModelPortaliQ12697.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000063001.
InParanoidiQ12697.
KOiK14951.
OMAiNIRTIMC.
OrthoDBiEOG092C0IZK.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 2 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006544. P-type_TPase_V.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF12409. P5-ATPase. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
TIGRFAMsiTIGR01494. ATPase_P-type. 2 hits.
TIGR01657. P-ATPase-V. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12697-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIPSSNQIQ HGQRSERNRR MPRASFSSTA TTSTAATLTS AMVLDQNNSE
60 70 80 90 100
PYAGATFEAV PSSIVSFHHP HSFQSSNLPS PHSSGNLEQR GRRLTESEPL
110 120 130 140 150
VLSSAEQSRS SSRNPSHFRF FTQEQISNAE GASTLENTDY DMAWDATPAY
160 170 180 190 200
EQDRIYGTGL SSRRSSIRSF SRASSLSNAK SYGSFSKRGR SGSRAPQRLG
210 220 230 240 250
ENSDTGFVYH SATHSSSSLS RYTTRERIPI ELESQTDEIL EDESSTHSLE
260 270 280 290 300
SSDSRRSASE NNRGSFSGHD DVHNQHSEYL KPDYHEKFYP QYAPNLHYQR
310 320 330 340 350
FYIAEEDLVI GIAAYQTSKF WYIIYNLCCF LTFGLVYLLT RWLPHLKVKL
360 370 380 390 400
YGVKVPLAKA EWVVIENEFG EFVIQPIDRQ WYNRPLSTVL PFENYPNPSY
410 420 430 440 450
EPNDINLSHH HANEINPNVP ILITFEYRYI KFIYSPLDDL FKTNNNWIDP
460 470 480 490 500
DWVDLSTVSN GLTKGVQEDR ELAFGKNQIN LRMKTTSEIL FNEVLHPFYV
510 520 530 540 550
FQVFSIILWG IDEYYYYAAC IFLISVLSIF DSLNEQKKVS RNLAEMSHFH
560 570 580 590 600
CDVRVLRDKF WTTISSSELV PGDIYEVSDP NITILPCDSI LLSSDCIVNE
610 620 630 640 650
SMLTGESVPV SKFPATEETM YQLCDDFQST QISSFVSKSF LYNGTNIIRA
660 670 680 690 700
RIAPGQTAAL AMVVRTGFST TKGSLVRSMV FPKPTGFKFY RDSFKYIGFM
710 720 730 740 750
SLIAIFGFCV SCVQFIKLGL DKKTMILRAL DIITIVVPPA LPATLTIGTN
760 770 780 790 800
FALSRLKEKG IFCISPTRLN ISGKIDVMCF DKTGTLTEDG LDVLGVQISE
810 820 830 840 850
PNGVRGQKFG ELLSDIRQVF PKFSLNDCSS PLDFKSRNFF MSLLTCHSLR
860 870 880 890 900
SVDGNLLGDP LDFKMFQFTG WSFEEDFQKR AFHSLYEGRH EDDVFPENSE
910 920 930 940 950
IIPAVVHPDS NNRENTFTDN DPHNFLGVVR SFEFLSELRR MSVIVKTNND
960 970 980 990 1000
DVYWSFTKGA PEVISEICNK STLPADFEEV LRCYTHNGYR VIACAGKTLP
1010 1020 1030 1040 1050
KRTWLYSQKV SREEVESNLE FLGFIIFQNK LKKETSETLK SLQDANIRTI
1060 1070 1080 1090 1100
MCTGDNILTA ISVGREAGLI QCSRVYVPSI NDTPLHGEPV IVWRDVNEPD
1110 1120 1130 1140 1150
KILDTKTLKP VKLGNNSVES LRECNYTLAV SGDVFRLLFR DENEIPEEYL
1160 1170 1180 1190 1200
NEILLNSSIY ARMSPDEKHE LMIQLQKLDY TVGFCGDGAN DCGALKAADV
1210 1220 1230 1240 1250
GISLSEAEAS VAAPFTSKIF NISCVLDVIR EGRAALVTSF ACFQYMSLYS
1260 1270 1280 1290 1300
AIQFITITIL YSRGSNLGDF QFLYIDLLLI VPIAICMSWS KSYEKIDKKR
1310 1320 1330 1340 1350
PSANLVSPKI LVPLLISVFL VFLFQFIPWI IVQKMSWYIK PIVGGDDAVQ
1360 1370 1380 1390 1400
SSDNTVLFFV SNFQYILTAI VLSVGPPYRE PMSKNFEFIV DITVSIGASL
1410 1420 1430 1440 1450
LLMTLDTESY LGKMLQLTPI SNSFTMFIIV WVILNYYAQL YIPPSIKGWL
1460 1470
KKKKSSKKYK LLIQEEMKLK EV
Length:1,472
Mass (Da):166,750
Last modified:November 1, 1997 - v1
Checksum:i6738AC22E561A4A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75199 Genomic DNA. Translation: CAA99518.1.
BK006948 Genomic DNA. Translation: DAA11055.1.
PIRiS67195.
RefSeqiNP_014934.1. NM_001183710.1.

Genome annotation databases

EnsemblFungiiYOR291W; YOR291W; YOR291W.
GeneIDi854466.
KEGGisce:YOR291W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75199 Genomic DNA. Translation: CAA99518.1.
BK006948 Genomic DNA. Translation: DAA11055.1.
PIRiS67195.
RefSeqiNP_014934.1. NM_001183710.1.

3D structure databases

ProteinModelPortaliQ12697.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34679. 76 interactors.
MINTiMINT-4503690.

Protein family/group databases

TCDBi3.A.3.10.8. the p-type atpase (p-atpase) superfamily.

PTM databases

iPTMnetiQ12697.

Proteomic databases

MaxQBiQ12697.
PRIDEiQ12697.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR291W; YOR291W; YOR291W.
GeneIDi854466.
KEGGisce:YOR291W.

Organism-specific databases

EuPathDBiFungiDB:YOR291W.
SGDiS000005817. YPK9.

Phylogenomic databases

GeneTreeiENSGT00530000063001.
InParanoidiQ12697.
KOiK14951.
OMAiNIRTIMC.
OrthoDBiEOG092C0IZK.

Enzyme and pathway databases

BioCyciYEAST:G3O-33776-MONOMER.
ReactomeiR-SCE-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiQ12697.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 2 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006544. P-type_TPase_V.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF12409. P5-ATPase. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
TIGRFAMsiTIGR01494. ATPase_P-type. 2 hits.
TIGR01657. P-ATPase-V. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYPK9_YEAST
AccessioniPrimary (citable) accession number: Q12697
Secondary accession number(s): D6W2Y9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 5, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.