Q12691 (ATN5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium transport ATPase 5
EC=3.6.3.7

Gene names

Name:	ENA5
Ordered Locus Names:	YDR038C
ORF Names:	YD9673.10c

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1091 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of sodium or lithium ions to allow salt tolerance By similarity.
Catalytic activity	ATP + H2O + Na+(In) = ADP + phosphate + Na+(Out).
Subcellular location	Cell membrane; Multi-pass membrane protein Ref.3.
Miscellaneous	Present with 606 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IID subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Sodium transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Magnesium Nucleotide-binding Sodium
Molecular function	Hydrolase
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane enriched fraction Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: InterPro sodium-exporting ATPase activity, phosphorylative mechanism Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1091	1091	Sodium transport ATPase 5		PRO_0000046244
Regions
	Topological domain	1 – 63	63	Cytoplasmic Potential
	Transmembrane	64 – 84	21	Helical; Potential
	Topological domain	85 – 90	6	Extracellular Potential
	Transmembrane	91 – 111	21	Helical; Potential
	Topological domain	112 – 282	171	Cytoplasmic Potential
	Transmembrane	283 – 303	21	Helical; Potential
	Topological domain	304 – 312	9	Extracellular Potential
	Transmembrane	313 – 333	21	Helical; Potential
	Topological domain	334 – 815	482	Cytoplasmic Potential
	Transmembrane	816 – 836	21	Helical; Potential
	Topological domain	837 – 848	12	Extracellular Potential
	Transmembrane	849 – 869	21	Helical; Potential
	Topological domain	870 – 885	16	Cytoplasmic Potential
	Transmembrane	886 – 906	21	Helical; Potential
	Topological domain	907 – 943	37	Extracellular Potential
	Transmembrane	944 – 964	21	Helical; Potential
	Topological domain	965 – 991	27	Cytoplasmic Potential
	Transmembrane	992 – 1012	21	Helical; Potential
	Topological domain	1013 – 1021	9	Extracellular Potential
	Transmembrane	1022 – 1042	21	Helical; Potential
	Topological domain	1043 – 1091	49	Cytoplasmic Potential
Sites
	Active site	369	1	4-aspartylphosphate intermediate By similarity
Amino acid modifications
	Modified residue	177	1	Phosphoserine Ref.7
	Cross-link	196		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q12691 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 66CEF9D7FE2549F1	FASTA	1,091	120,296
	10 20 30 40 50 60 MSEGTVKENN NEEFNAYHTL TTEEAAEFIG TSLTEGLTQD ESLRRLKAVG ENTLGDDTKI 70 80 90 100 110 120 DYKAMVLHQV CNAMIMVLVI SMAISFAVRD WITGGVISFV IAVNVLIGLV QEYKATKTMN 130 140 150 160 170 180 SLKNLSSPNA HVIRNGKSET INSKDVVPGD ICLVKVGDTI PADLRLIETK NFDTDESLLT 190 200 210 220 230 240 GESLPVSKDA NLVFGKEEET SVGDRLNLAF SSSAVVKGRA KGIVIKTALN SEIGKIAKSL 250 260 270 280 290 300 QGDSGLISRD PSKSWLQNTW ISTKKVTGAF LGTNVGTPLH RKLSKLAVLL FWIAVLFAII 310 320 330 340 350 360 VMASQKFDVD KRVAIYAICV ALSMIPSSLV VVLTITMSVG AAVMVSRNVI VRKLDSLEAL 370 380 390 400 410 420 GAVNDICSDK TGTLTQGKML ARQIWIPRFG TITISNSDDP FNPNEGNVSL IPRFSPYEYS 430 440 450 460 470 480 HNEDGDVGIL QNFKDRLYEK DLPEDIDMDL FQKWLETATL ANIATVFKDD ATDCWKAHGD 490 500 510 520 530 540 PTEIAIQVFA TKMDLPHNAL TGEKSTNQSN ENDQSSLSQH NEKPGSAQFE HIAEFPFDST 550 560 570 580 590 600 VKRMSSVYYN NHNETYNIYG KGAFESIISC CSSWYGKDGV KITPLTDCDV ETIRKNVYSL 610 620 630 640 650 660 SNEGLRVLGF ASKSFTKDQV NDDQLKNITS NRATAESDLV FLGLIGIYDP PRNETAGAVK 670 680 690 700 710 720 KFHQAGINVH MLTGDFVGTA KAIAQEVGIL PTNLYHYSQE IVDSMVMTGS QFDGLSEEEV 730 740 750 760 770 780 DDLPVLPLVI ARCSPQTKVR MIEALHRRKK FCAMTGDGVN DSPSLKMANV GIAMGINGSD 790 800 810 820 830 840 VSKEASDIVL SDDNFASILN AVEEGRRMTD NIQKFVLQLL AENVAQALYL IIGLVFRDEN 850 860 870 880 890 900 GKSVFPLSPV EVLWIIVVTS CFPAMGLGLE KAAPDLMDRP PNDSEVGIFT WEVIIDTFAY 910 920 930 940 950 960 GIIMTGSCMA SFTGSLYGIN SGRLGHDCDG TYNSSCRDVY RSRSAAFATM TWCALILAWE 970 980 990 1000 1010 1020 VVDMRRSFFR MHPDTDSPVK EFFRSIWGNQ FLFWSIIFGF VSAFPVVYIP VINDKVFLHK 1030 1040 1050 1060 1070 1080 PIGAEWGLAI AFTIAFWIGA ELYKCGKRRY FKTQRAHNSE NDLERSSKHD PFEAYSTSTT 1090 LQSEINISVK H

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery." Hitchcock A.L., Auld K., Gygi S.P., Silver P.A. Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed: 14557538] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-196, MASS SPECTROMETRY.
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-177, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z68196 Genomic DNA. Translation: CAA92377.1. Z74334 Genomic DNA. Translation: CAA98864.1. Z74335 Genomic DNA. Translation: CAA98865.1. BK006938 Genomic DNA. Translation: DAA11886.1.
PIR	S67852.
RefSeq	NP_010323.1. NM_001180346.1.
3D structure databases
ProteinModelPortal	Q12691.
SMR	Q12691. Positions 117-244, 478-649, 737-803.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5253N.
IntAct	Q12691. 1 interaction.
MINT	MINT-547436.
STRING	Q12691.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR038C; YDR038C; YDR038C.
GeneID	851608.
KEGG	sce:YDR038C.
Organism-specific databases
CYGD	YDR038c.
SGD	S000002445. ENA5.
Phylogenomic databases
eggNOG	fuNOG05186.
HOGENOM	HBG456486.
OrthoDB	EOG46X2HG.
Gene expression databases
ArrayExpress	Q12691.
Genevestigator	Q12691.
GermOnline	YDR038C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR023306. ATPase_cation_domN. IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR023300. ATPase_P-typ_cyto_domA. IPR023299. ATPase_P-typ_cyto_domN. IPR001757. ATPase_P-typ_ion-transptr. IPR006414. ATPase_P-typ_K/Na-efflux_fun. IPR018303. ATPase_P-typ_P_site. IPR023298. ATPase_P-typ_TM_dom. IPR005834. Dehalogen-like_hydro. IPR023214. HAD-like_dom. [Graphical view]
Gene3D	G3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 1 hit. G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 2 hits. G3DSA:1.20.1110.10. ATPase_P-typ_TM_dom. 1 hit.
PANTHER	PTHR24093:SF83. PTHR24093:SF83. 1 hit.
Pfam	PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
SMART	SM00831. Cation_ATPase_N. 1 hit. [Graphical view]
SUPFAM	SSF81660. ATPase_cation_domN. 1 hit. SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01523. ATPase-IID_K-Na. 1 hit. TIGR01494. ATPase_P-type. 3 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	969118.

Entry information

Entry name

ATN5_YEAST

Accession

Primary (citable) accession number: Q12691
Secondary accession number(s): D6VS26, E9PAF4, P89901

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program