Q12679 (GUNA_ASPKW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase A
EC=3.2.1.4

Alternative name(s):
CMCase-I
Carboxymethylcellulase
Cellulase A
Endo-1,4-beta-glucanase A

Gene names

Name:	cekA
ORF Names:	AKAW_05147

Organism

Aspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus awamori var. kawachi) [Complete proteome]

Taxonomic identifier

1033177 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus kawachii

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Has carboxylmethylcellulase activity.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 12 (cellulase H) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 239	223	Endoglucanase A		PRO_0000008019
Experimental info
	Sequence conflict	217	1	F → C in BAA02297. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q12679 [UniParc]. Last modified March 21, 2012. Version 2. Checksum: E3B0304A13827066	FASTA	239	25,813
	10 20 30 40 50 60 MKLSMTLSLF AATAMGQTMC SQYDSASSPP YSVNQNLWGE YQGTGSQCVY VDKLSSSGAS 70 80 90 100 110 120 WHTKWTWSGG EGTVKSYSNS GLTFDKKLVS DVSSIPTSVT WSQDDTNVQA DVSYDLFTAA 130 140 150 160 170 180 NADHATSSGD YELMIWLARY GSVQPIGKQI ATATVGGKSW EVWYGTSTQA GAEQKTYSFV 190 200 210 220 230 AGSPINSWSG DIKDFFNYLT QNQGFPASSQ HLITLQFGTE PFTGGPATFT VDNWTASVN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of cellulase cDNA from Aspergillus kawachii and its expression in Saccharomyces cerevisiae." Sakamoto S., Tamura G., Ito K., Ishikawa T., Iwano K., Nishiya N. Curr. Genet. 27:435-439(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: NBRC 4308.
[2]	"Genome sequence of the white koji mold Aspergillus kawachii IFO 4308, used for brewing the Japanese distilled spirit shochu." Futagami T., Mori K., Yamashita A., Wada S., Kajiwara Y., Takashita H., Omori T., Takegawa K., Tashiro K., Kuhara S., Goto M. Eukaryot. Cell 10:1586-1587(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NBRC 4308.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D12901 mRNA. Translation: BAA02297.1. DF126458 Genomic DNA. Translation: GAA87033.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH12. Glycoside Hydrolase Family 12.
mycoCLAP	EGL12A_ASPKA.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.60.120.180. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013319. Glyco_hydro_11/12. IPR002594. Glyco_hydro_12. [Graphical view]
Pfam	PF01670. Glyco_hydro_12. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GUNA_ASPKW

Accession

Primary (citable) accession number: Q12679
Secondary accession number(s): G7XJV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	March 21, 2012
Last modified:	April 3, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program