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Q12676 (FOLD_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Folylpolyglutamate synthase
EC=6.3.2.17
Alternative name(s):
Folylpoly-gamma-glutamate synthetase
Short name=FPGS
Tetrahydrofolylpolyglutamate synthase
Short name=Tetrahydrofolate synthase
Gene names
Name:FOL3
Ordered Locus Names:YMR113W
ORF Names:YM9718.12
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length427 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Conversion of folates to polyglutamate derivatives.

Catalytic activity

ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1).

Pathway

Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.

Subcellular location

Cytoplasm Ref.4.

Miscellaneous

Present with 2340 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the folylpolyglutamate synthase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processone-carbon metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from direct assay Ref.4. Source: SGD

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

dihydrofolate synthase activity

Inferred from mutant phenotype. Source: SGD

tetrahydrofolylpolyglutamate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 427427Folylpolyglutamate synthase
PRO_0000168308

Regions

Nucleotide binding30 – 367ATP Potential

Sequences

Sequence LengthMass (Da)Tools
Q12676 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: C3307CEF43BE1F30

FASTA42747,851
        10         20         30         40         50         60 
MAIELGLSRI TKLLEHLGNP QNSLRVLHIA GTNGKGSVCT YLSSVLQQKS YQIGKFTTPH 

        70         80         90        100        110        120 
LVHVTDSITI NNKPIPLERY QNIRLQLEAL NKSHSLKCTE FELLTCTAFK YFYDVQCQWC 

       130        140        150        160        170        180 
VIEVGLGGRL DATNVIPGAN KACCGITKIS LDHESFLGNT LSEISKEKAG IITEGVPFTV 

       190        200        210        220        230        240 
IDGTNEASVI NVVKERCKAL GSELSVTDSQ LNGNMIDTNS WGCFDLAKLP LNGEYQIFNL 

       250        260        270        280        290        300 
RVAMGMLDYL QMNELIDITK NEVSTRLAKV DWPGRLYRMD YRFDKVSNRT VPILMDGAHN 

       310        320        330        340        350        360 
GSAAVELVKY LRKEYGNQPL TFVMAVTHGK NLEPLLQPLL RPIDQVILTR FNNVEGMPWI 

       370        380        390        400        410        420 
HATDPEEIKD FILTQGYTKE IVIENDLHQV LPSLAHVSDE QRRPIVVCGS LYLCGELLRI 


HNSHLRN

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed: 9169872] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Polyglutamylation of folate coenzymes is necessary for methionine biosynthesis and maintenance of intact mitochondrial genome in Saccharomyces cerevisiae."
Cherest H., Thomas D., Surdin-Kerjan Y.
J. Biol. Chem. 275:14056-14063(2000) [PubMed: 10799479] [Abstract]
Cited for: CHARACTERIZATION.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed: 14562095] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z49702 Genomic DNA. Translation: CAA89750.1.
BK006946 Genomic DNA. Translation: DAA10010.1.
PIRS54574.
RefSeqNP_013831.1. NM_001182613.1.

3D structure databases

ProteinModelPortalQ12676.
SMRQ12676. Positions 2-422.
ModBaseSearch...

Protein-protein interaction databases

IntActQ12676. 2 interactions.
MINTMINT-2782471.
STRINGQ12676.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR113W; YMR113W; YMR113W.
GeneID855140.
KEGGsce:YMR113W.
NMPDRfig|4932.3.peg.4879.

Organism-specific databases

CYGDYMR113w.
SGDS000004719. FOL3.

Phylogenomic databases

eggNOGfuNOG08867.
GeneTreeEFGT00050000004649.
HOGENOMHBG744947.
OMANSSPRAM.
OrthoDBEOG4BZRBJ.

Gene expression databases

ArrayExpressQ12676.
GenevestigatorQ12676.
GermOnlineYMR113W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR001645. Folylpolyglutamate_synth.
IPR018109. Folylpolyglutamate_synth_CS.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
KOK01930.
PANTHERPTHR11136. Fpolygl_synthtse. 1 hit.
PfamPF08245. Mur_ligase_M. 1 hit.
[Graphical view]
PIRSFPIRSF001563. Folylpolyglu_synth. 1 hit.
SUPFAMSSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMsTIGR01499. FolC. 1 hit.
PROSITEPS01011. FOLYLPOLYGLU_SYNT_1. 1 hit.
PS01012. FOLYLPOLYGLU_SYNT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978528.

Entry information

Entry nameFOLD_YEAST
AccessionPrimary (citable) accession number: Q12676
Secondary accession number(s): D6VZT6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 25, 2012
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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