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Protein

Dihydrofolate synthetase

Gene

FOL3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Glutamate-adding enzyme which catalyzes the binding of the first glutamyl side chain to dihydropteroate. Leads to the de nove synthesis of tetrahydrofolate.1 Publication

Catalytic activityi

ATP + 7,8-dihydropteroate + L-glutamate = ADP + phosphate + 7,8-dihydropteroylglutamate.

Pathwayi: tetrahydrofolylpolyglutamate biosynthesis

This protein is involved in the pathway tetrahydrofolylpolyglutamate biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolylpolyglutamate biosynthesis and in Cofactor biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi30 – 367ATPSequence analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • dihydrofolate synthase activity Source: SGD
  • tetrahydrofolylpolyglutamate synthase activity Source: InterPro

GO - Biological processi

  • dihydrofolate biosynthetic process Source: GOC
  • folic acid-containing compound biosynthetic process Source: SGD
  • one-carbon metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YMR113W-MONOMER.
UniPathwayiUPA00850.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate synthetase (EC:6.3.2.12)
Short name:
DHFS
Gene namesi
Name:FOL3
Ordered Locus Names:YMR113W
ORF Names:YM9718.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR113W.
SGDiS000004719. FOL3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427Dihydrofolate synthetasePRO_0000168308Add
BLAST

Proteomic databases

MaxQBiQ12676.

Interactioni

Protein-protein interaction databases

BioGridi35289. 6 interactions.
IntActiQ12676. 2 interactions.
MINTiMINT-2782471.

Structurei

3D structure databases

ProteinModelPortaliQ12676.
SMRiQ12676. Positions 3-413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the folylpolyglutamate synthase family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000075841.
HOGENOMiHOG000019981.
InParanoidiQ12676.
KOiK01930.
OMAiMLSTKDH.
OrthoDBiEOG7NKKX2.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.90.190.20. 1 hit.
InterProiIPR001645. Folylpolyglutamate_synth.
IPR018109. Folylpolyglutamate_synth_CS.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
[Graphical view]
PANTHERiPTHR11136. PTHR11136. 1 hit.
PfamiPF08245. Mur_ligase_M. 1 hit.
[Graphical view]
PIRSFiPIRSF001563. Folylpolyglu_synth. 1 hit.
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01499. folC. 1 hit.
PROSITEiPS01011. FOLYLPOLYGLU_SYNT_1. 1 hit.
PS01012. FOLYLPOLYGLU_SYNT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12676-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIELGLSRI TKLLEHLGNP QNSLRVLHIA GTNGKGSVCT YLSSVLQQKS
60 70 80 90 100
YQIGKFTTPH LVHVTDSITI NNKPIPLERY QNIRLQLEAL NKSHSLKCTE
110 120 130 140 150
FELLTCTAFK YFYDVQCQWC VIEVGLGGRL DATNVIPGAN KACCGITKIS
160 170 180 190 200
LDHESFLGNT LSEISKEKAG IITEGVPFTV IDGTNEASVI NVVKERCKAL
210 220 230 240 250
GSELSVTDSQ LNGNMIDTNS WGCFDLAKLP LNGEYQIFNL RVAMGMLDYL
260 270 280 290 300
QMNELIDITK NEVSTRLAKV DWPGRLYRMD YRFDKVSNRT VPILMDGAHN
310 320 330 340 350
GSAAVELVKY LRKEYGNQPL TFVMAVTHGK NLEPLLQPLL RPIDQVILTR
360 370 380 390 400
FNNVEGMPWI HATDPEEIKD FILTQGYTKE IVIENDLHQV LPSLAHVSDE
410 420
QRRPIVVCGS LYLCGELLRI HNSHLRN
Length:427
Mass (Da):47,851
Last modified:November 1, 1997 - v1
Checksum:iC3307CEF43BE1F30
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49702 Genomic DNA. Translation: CAA89750.1.
BK006946 Genomic DNA. Translation: DAA10010.1.
PIRiS54574.
RefSeqiNP_013831.1. NM_001182613.1.

Genome annotation databases

EnsemblFungiiYMR113W; YMR113W; YMR113W.
GeneIDi855140.
KEGGisce:YMR113W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49702 Genomic DNA. Translation: CAA89750.1.
BK006946 Genomic DNA. Translation: DAA10010.1.
PIRiS54574.
RefSeqiNP_013831.1. NM_001182613.1.

3D structure databases

ProteinModelPortaliQ12676.
SMRiQ12676. Positions 3-413.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35289. 6 interactions.
IntActiQ12676. 2 interactions.
MINTiMINT-2782471.

Proteomic databases

MaxQBiQ12676.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR113W; YMR113W; YMR113W.
GeneIDi855140.
KEGGisce:YMR113W.

Organism-specific databases

EuPathDBiFungiDB:YMR113W.
SGDiS000004719. FOL3.

Phylogenomic databases

GeneTreeiENSGT00550000075841.
HOGENOMiHOG000019981.
InParanoidiQ12676.
KOiK01930.
OMAiMLSTKDH.
OrthoDBiEOG7NKKX2.

Enzyme and pathway databases

UniPathwayiUPA00850.
BioCyciYEAST:YMR113W-MONOMER.

Miscellaneous databases

NextBioi978528.
PROiQ12676.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.90.190.20. 1 hit.
InterProiIPR001645. Folylpolyglutamate_synth.
IPR018109. Folylpolyglutamate_synth_CS.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
[Graphical view]
PANTHERiPTHR11136. PTHR11136. 1 hit.
PfamiPF08245. Mur_ligase_M. 1 hit.
[Graphical view]
PIRSFiPIRSF001563. Folylpolyglu_synth. 1 hit.
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01499. folC. 1 hit.
PROSITEiPS01011. FOLYLPOLYGLU_SYNT_1. 1 hit.
PS01012. FOLYLPOLYGLU_SYNT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Polyglutamylation of folate coenzymes is necessary for methionine biosynthesis and maintenance of intact mitochondrial genome in Saccharomyces cerevisiae."
    Cherest H., Thomas D., Surdin-Kerjan Y.
    J. Biol. Chem. 275:14056-14063(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "Folic acid utilisation related to sulfa drug resistance in Saccharomyces cerevisiae."
    Bayly A.M., Berglez J.M., Patel O., Castelli L.A., Hankins E.G., Coloe P., Hopkins Sibley C., Macreadie I.G.
    FEMS Microbiol. Lett. 204:387-390(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFOLD_YEAST
AccessioniPrimary (citable) accession number: Q12676
Secondary accession number(s): D6VZT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 11, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2340 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.