Reviewed, UniProtKB/Swiss-Prot Q12676 (FOLD_YEAST)

Names and origin

Protein names

Recommended name:
    Folylpolyglutamate synthase
    EC=6.3.2.17
Alternative name(s):
    Folylpoly-gamma-glutamate synthetase
      Short name=FPGS
    Tetrahydrofolylpolyglutamate synthase
      Short name=Tetrahydrofolate synthase

Gene names

Name:	FOL3
Ordered Locus Names:	YMR113W
ORF Names:	YM9718.12

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Conversion of folates to polyglutamate derivatives.
Catalytic activity	ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1).
Pathway	Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.
Subcellular location	Cytoplasm. Ref.3
Miscellaneous	Present with 2340 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the folylpolyglutamate synthase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from mutant phenotype. Source: SGD one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Ref.3 Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dihydrofolate synthase activity Inferred from mutant phenotype. Source: SGD tetrahydrofolylpolyglutamate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Folylpolyglutamate synthase		PRO_0000168308
Regions
	Nucleotide binding	30 – 36	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q12676-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: C3307CEF43BE1F30	FASTA	427	47,851
	10 20 30 40 50 60 MAIELGLSRI TKLLEHLGNP QNSLRVLHIA GTNGKGSVCT YLSSVLQQKS YQIGKFTTPH 70 80 90 100 110 120 LVHVTDSITI NNKPIPLERY QNIRLQLEAL NKSHSLKCTE FELLTCTAFK YFYDVQCQWC 130 140 150 160 170 180 VIEVGLGGRL DATNVIPGAN KACCGITKIS LDHESFLGNT LSEISKEKAG IITEGVPFTV 190 200 210 220 230 240 IDGTNEASVI NVVKERCKAL GSELSVTDSQ LNGNMIDTNS WGCFDLAKLP LNGEYQIFNL 250 260 270 280 290 300 RVAMGMLDYL QMNELIDITK NEVSTRLAKV DWPGRLYRMD YRFDKVSNRT VPILMDGAHN 310 320 330 340 350 360 GSAAVELVKY LRKEYGNQPL TFVMAVTHGK NLEPLLQPLL RPIDQVILTR FNNVEGMPWI 370 380 390 400 410 420 HATDPEEIKD FILTQGYTKE IVIENDLHQV LPSLAHVSDE QRRPIVVCGS LYLCGELLRI HNSHLRN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Polyglutamylation of folate coenzymes is necessary for methionine biosynthesis and maintenance of intact mitochondrial genome in Saccharomyces cerevisiae." Cherest H., Thomas D., Surdin-Kerjan Y. J. Biol. Chem. 275:14056-14063(2000) [PubMed: 10799479] [Abstract] Cited for: CHARACTERIZATION.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49702 Genomic DNA. Translation: CAA89750.1.
PIR	S54574.
RefSeq	NP_013831.1.
3D structure databases
HSSP	HSSP built from PDB template 1FGS based on UniProtKB P15925.
ModBase	Search...
Genome annotation databases
Ensembl	YMR113W. Saccharomyces cerevisiae. [Contig view]
GeneID	855140.
GenomeReviews	Gene locus YMR113W in contig Z71257_GR.
KEGG	sce:YMR113W.
NMPDR	fig|4932.3.peg.4879.
Organism-specific databases
CYGD	YMR113w.
SGD	S000004719. FOL3.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q12676.
OMA	Q12676. HPYTHAV.
Enzyme and pathway databases
BRENDA	6.3.2.17. 250.
Gene expression databases
ArrayExpress	Q12676.
GermOnline	YMR113W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018109. Folylpolyglutamate_synth_CS. IPR001645. Fpolygl_synthtse. IPR013221. Mur_ligase_cen. [Graphical view]
Gene3D	G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
PANTHER	PTHR11136. Fpolygl_synthtse. 1 hit.
Pfam	PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01499. folC. 1 hit.
PROSITE	PS01011. FOLYLPOLYGLU_SYNT_1. 1 hit. PS01012. FOLYLPOLYGLU_SYNT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	978528.

Entry information

Entry name

FOLD_YEAST

Accession

Primary (citable) accession number: Q12676

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)