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Protein

Probable phospholipid-transporting ATPase DNF3

Gene

DNF3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids.Curated

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei566 – 56614-aspartylphosphate intermediateCurated

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • magnesium ion binding Source: InterPro
  • phospholipid-translocating ATPase activity Source: SGD

GO - Biological processi

  • intracellular protein transport Source: SGD
  • phospholipid translocation Source: SGD
  • response to pheromone involved in conjugation with cellular fusion Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32852-MONOMER.
BRENDAi3.6.3.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phospholipid-transporting ATPase DNF3 (EC:3.6.3.1)
Gene namesi
Name:DNF3
Ordered Locus Names:YMR162C
ORF Names:YM8520.11C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR162C.
SGDiS000004772. DNF3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 164164ExtracellularSequence analysisAdd
BLAST
Transmembranei165 – 18521HelicalSequence analysisAdd
BLAST
Topological domaini186 – 451266CytoplasmicSequence analysisAdd
BLAST
Transmembranei452 – 47221HelicalSequence analysisAdd
BLAST
Topological domaini473 – 49523ExtracellularSequence analysisAdd
BLAST
Transmembranei496 – 51621HelicalSequence analysisAdd
BLAST
Topological domaini517 – 1157641CytoplasmicSequence analysisAdd
BLAST
Transmembranei1158 – 117821HelicalSequence analysisAdd
BLAST
Topological domaini1179 – 1318140ExtracellularSequence analysisAdd
BLAST
Transmembranei1319 – 133921HelicalSequence analysisAdd
BLAST
Topological domaini1340 – 136526CytoplasmicSequence analysisAdd
BLAST
Transmembranei1366 – 138621HelicalSequence analysisAdd
BLAST
Topological domaini1387 – 13959ExtracellularSequence analysis
Transmembranei1396 – 141621HelicalSequence analysisAdd
BLAST
Topological domaini1417 – 143216CytoplasmicSequence analysisAdd
BLAST
Transmembranei1433 – 145321HelicalSequence analysisAdd
BLAST
Topological domaini1454 – 147320ExtracellularSequence analysisAdd
BLAST
Transmembranei1474 – 149421HelicalSequence analysisAdd
BLAST
Topological domaini1495 – 1656162CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mating projection tip membrane Source: SGD
  • trans-Golgi network Source: SGD
  • trans-Golgi network transport vesicle Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16561656Probable phospholipid-transporting ATPase DNF3PRO_0000046237Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei627 – 6271PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12674.

PTM databases

iPTMnetiQ12674.

Interactioni

Protein-protein interaction databases

BioGridi35339. 31 interactions.
IntActiQ12674. 6 interactions.
MINTiMINT-4498184.

Structurei

3D structure databases

ProteinModelPortaliQ12674.
SMRiQ12674. Positions 1079-1251.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00840000130944.
HOGENOMiHOG000190484.
InParanoidiQ12674.
KOiK01530.
OMAiCETRTAT.
OrthoDBiEOG092C0BQ4.

Family and domain databases

Gene3Di2.70.150.10. 3 hits.
3.40.1110.10. 3 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 4 hits.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12674-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIADGQRRR SSSLRTQMFN KHLYDKYRGR TDDEIELEDI NESKTFSGSD
60 70 80 90 100
NNDKDDRDET SGNYAAEEDY EMEEYGSPDV SYSIITKILD TILDRRRTFH
110 120 130 140 150
SKDGRHIPII LDHNAIEYKQ AATKRDGHLI DERFNKPYCD NRITSSRYTF
160 170 180 190 200
YSFLPRQLYA QFSKLANTYF FIVAVLQMIP GWSTTGTYTT IIPLCVFMGI
210 220 230 240 250
SMTREAWDDF RRHRLDKEEN NKPVGVLVKD GNNDAQEVYT LPSSVVSSTA
260 270 280 290 300
YLTKSAAAEN NPPLNDDRNS SQGHFLDTHF NNFELLKNKY NVHIHQKKWE
310 320 330 340 350
KLRVGDFVLL TQDDWVPADL LLLTCDGENS ECFVETMALD GETNLKSKQP
360 370 380 390 400
HPELNKLTKA ASGLANINAQ VTVEDPNIDL YNFEGNLELK NHRNDTIMKY
410 420 430 440 450
PLGPDNVIYR GSILRNTQNV VGMVIFSGEE TKIRMNALKN PRTKAPKLQR
460 470 480 490 500
KINMIIVFMV FVVATISLFS YLGHVLHKKK YIDQNKAWYL FQADAGVAPT
510 520 530 540 550
IMSFIIMYNT VIPLSLYVTM EIIKVVQSKM MEWDIDMYHA ETNTPCESRT
560 570 580 590 600
ATILEELGQV SYIFSDKTGT LTDNKMIFRK FSLCGSSWLH NVDLGNSEDN
610 620 630 640 650
FEDNRDNTNS LRLPPKAHNG SSIDVVSIGD QNVLDRLGFS DAPIEKGHRP
660 670 680 690 700
SLDNFPKSRN SIEYKGNSSA IYTGRPSMRS LFGKDNSHLS KQASVISPSE
710 720 730 740 750
TFSENIKSSF DLIQFIQRYP TALFSQKAKF FFLSLALCHS CLPKKTHNES
760 770 780 790 800
IGEDSIEYQS SSPDELALVT AARDLGYIVL NRNAQILTIK TFPDGFDGEA
810 820 830 840 850
KLENYEILNY IDFNSQRKRM SVLVRMPNQP NQVLLICKGA DNVIMERLHD
860 870 880 890 900
RELAAKKMAD ICTSTKERKD AEAELVLQQR KSLERMVDEE AMARTSLRNS
910 920 930 940 950
LSSVPRASLS LQAVRKSLSM KNSRTRDPEK QIDSIDQFLE TVKKSDQEIG
960 970 980 990 1000
SVVNKSRKSL HKQQIEKYGP RISIDGTHFP NNNVPIDTRK EGLQHDYDTE
1010 1020 1030 1040 1050
ILEHIGSDEL ILNEEYVIER TLQAIDEFST EGLRTLVYAY KWIDIGQYEN
1060 1070 1080 1090 1100
WNKRYHQAKT SLTDRKIKVD EAGAEIEDGL NLLGVTAIED KLQDGVSEAI
1110 1120 1130 1140 1150
EKIRRAGIKM WMLTGDKRET AINIGYSCML IKDYSTVVIL TTTDENIISK
1160 1170 1180 1190 1200
MNAVSQEVDS GNIAHCVVVI DGATMAMFEG NPTYMSVFVE LCTKTDSVIC
1210 1220 1230 1240 1250
CRASPSQKAL MVSNIRNTDP NLVTLAIGDG ANDIAMIQSA DIGVGIAGKE
1260 1270 1280 1290 1300
GLQASRVSDY SIGQFRFLLK LLFVHGRYNY IRTSKFMLCT FYKEITFYFT
1310 1320 1330 1340 1350
QLIYQRYTMF SGSSLYEPWS LSMFNTLFTS LPVLCIGMFE KDLKPMTLLT
1360 1370 1380 1390 1400
VPELYSYGRL SQGFNWLIFM EWVILATTNS LIITFLNVVM WGMSSLSDNT
1410 1420 1430 1440 1450
MYPLGLINFT AIVALINVKS QFVEMHNRNW LAFTSVVLSC GGWLVWCCAL
1460 1470 1480 1490 1500
PILNNTDQIY DVAYGFYNHF GKDITFWCTS LVLALLPITL DIVYKTFKVM
1510 1520 1530 1540 1550
IWPSDSDIFA ELEQKSDIRK KLELGAYSEM RQGWTWDKDP STFTRYTDKV
1560 1570 1580 1590 1600
LSRPRTNSRA SAKTHNSSIY SMSNGNVDHS SKKNFFGNSS KKSSERYEVL
1610 1620 1630 1640 1650
PSGKLIKRPS LKTQSSKDSI GGNITTKLTK KLKLPSRNVE DEDVNQIIQA

RLKDLE
Length:1,656
Mass (Da):188,319
Last modified:November 1, 1997 - v1
Checksum:iA20A823BEB401184
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49705 Genomic DNA. Translation: CAA89798.1.
BK006946 Genomic DNA. Translation: DAA10058.1.
PIRiS54520.
RefSeqiNP_013885.1. NM_001182666.1.

Genome annotation databases

EnsemblFungiiYMR162C; YMR162C; YMR162C.
GeneIDi855197.
KEGGisce:YMR162C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49705 Genomic DNA. Translation: CAA89798.1.
BK006946 Genomic DNA. Translation: DAA10058.1.
PIRiS54520.
RefSeqiNP_013885.1. NM_001182666.1.

3D structure databases

ProteinModelPortaliQ12674.
SMRiQ12674. Positions 1079-1251.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35339. 31 interactions.
IntActiQ12674. 6 interactions.
MINTiMINT-4498184.

PTM databases

iPTMnetiQ12674.

Proteomic databases

MaxQBiQ12674.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR162C; YMR162C; YMR162C.
GeneIDi855197.
KEGGisce:YMR162C.

Organism-specific databases

EuPathDBiFungiDB:YMR162C.
SGDiS000004772. DNF3.

Phylogenomic databases

GeneTreeiENSGT00840000130944.
HOGENOMiHOG000190484.
InParanoidiQ12674.
KOiK01530.
OMAiCETRTAT.
OrthoDBiEOG092C0BQ4.

Enzyme and pathway databases

BioCyciYEAST:G3O-32852-MONOMER.
BRENDAi3.6.3.1. 984.

Miscellaneous databases

PROiQ12674.

Family and domain databases

Gene3Di2.70.150.10. 3 hits.
3.40.1110.10. 3 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 4 hits.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATC8_YEAST
AccessioniPrimary (citable) accession number: Q12674
Secondary accession number(s): D6VZY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.