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Protein

Probable phospholipid-transporting ATPase DNF3

Gene

DNF3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids (Probable). Forms a heteromeric phospholipid translocase (PLT) DNF3-CRF1, implicated in the translocation of phospholipids from the outer to the inner leaflet of membrane bilayers. Shares an essential function for cell growth with PLTs DRS2-CDC50 and DNF1/2-LEM3. May be involved in transport from early endosomes to the trans-Golgi network (TGN) (Probable).Curated

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei5664-aspartylphosphate intermediateCurated1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • magnesium ion binding Source: InterPro
  • phospholipid-translocating ATPase activity Source: SGD

GO - Biological processi

  • endocytic recycling Source: GO_Central
  • Golgi vesicle budding Source: GO_Central
  • intracellular protein transport Source: SGD
  • phospholipid translocation Source: SGD
  • post-Golgi vesicle-mediated transport Source: GO_Central
  • response to pheromone involved in conjugation with cellular fusion Source: SGD

Keywordsi

Molecular functionHydrolase
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32852-MONOMER.
BRENDAi3.6.3.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phospholipid-transporting ATPase DNF3 (EC:3.6.3.1)
Alternative name(s):
Aminophospholipid translocase
Short name:
APT
Phospholipid translocase1 Publication
Short name:
PLT
Gene namesi
Name:DNF3
Ordered Locus Names:YMR162C
ORF Names:YM8520.11C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR162C.
SGDiS000004772. DNF3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 164Lumenal1 PublicationAdd BLAST164
Transmembranei165 – 185HelicalSequence analysisAdd BLAST21
Topological domaini186 – 451CytoplasmicSequence analysisAdd BLAST266
Transmembranei452 – 472HelicalSequence analysisAdd BLAST21
Topological domaini473 – 495Lumenal1 PublicationAdd BLAST23
Transmembranei496 – 516HelicalSequence analysisAdd BLAST21
Topological domaini517 – 1157CytoplasmicSequence analysisAdd BLAST641
Transmembranei1158 – 1178HelicalSequence analysisAdd BLAST21
Topological domaini1179 – 1318Lumenal1 PublicationAdd BLAST140
Transmembranei1319 – 1339HelicalSequence analysisAdd BLAST21
Topological domaini1340 – 1365CytoplasmicSequence analysisAdd BLAST26
Transmembranei1366 – 1386HelicalSequence analysisAdd BLAST21
Topological domaini1387 – 1395Lumenal1 Publication9
Transmembranei1396 – 1416HelicalSequence analysisAdd BLAST21
Topological domaini1417 – 1432CytoplasmicSequence analysisAdd BLAST16
Transmembranei1433 – 1453HelicalSequence analysisAdd BLAST21
Topological domaini1454 – 1473Lumenal1 PublicationAdd BLAST20
Transmembranei1474 – 1494HelicalSequence analysisAdd BLAST21
Topological domaini1495 – 1656Cytoplasmic1 PublicationAdd BLAST162

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462371 – 1656Probable phospholipid-transporting ATPase DNF3Add BLAST1656

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei627PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12674.
PRIDEiQ12674.

PTM databases

iPTMnetiQ12674.

Interactioni

Subunit structurei

Interacts with YNR048W/CRF1; interaction is required for proper expression and endoplasmic reticulum (ER) export of either partner.2 Publications

Protein-protein interaction databases

BioGridi35339. 60 interactors.
IntActiQ12674. 7 interactors.
MINTiMINT-4498184.
STRINGi4932.YMR162C.

Structurei

3D structure databases

ProteinModelPortaliQ12674.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00900000143102.
HOGENOMiHOG000190484.
InParanoidiQ12674.
KOiK01530.
OMAiESWSLSM.
OrthoDBiEOG092C0BQ4.

Family and domain databases

Gene3Di3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiView protein in InterPro
IPR023299. ATPase_P-typ_cyto_dom_N.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom_sf.
IPR008250. ATPase_P-typ_transduc_dom_A_sf.
IPR036412. HAD-like_sf.
IPR023214. HAD_sf.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
PANTHERiPTHR24092. PTHR24092. 1 hit.
PfamiView protein in Pfam
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81653. SSF81653. 2 hits.
SSF81660. SSF81660. 1 hit.
SSF81665. SSF81665. 3 hits.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiView protein in PROSITE
PS00154. ATPASE_E1_E2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12674-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIADGQRRR SSSLRTQMFN KHLYDKYRGR TDDEIELEDI NESKTFSGSD
60 70 80 90 100
NNDKDDRDET SGNYAAEEDY EMEEYGSPDV SYSIITKILD TILDRRRTFH
110 120 130 140 150
SKDGRHIPII LDHNAIEYKQ AATKRDGHLI DERFNKPYCD NRITSSRYTF
160 170 180 190 200
YSFLPRQLYA QFSKLANTYF FIVAVLQMIP GWSTTGTYTT IIPLCVFMGI
210 220 230 240 250
SMTREAWDDF RRHRLDKEEN NKPVGVLVKD GNNDAQEVYT LPSSVVSSTA
260 270 280 290 300
YLTKSAAAEN NPPLNDDRNS SQGHFLDTHF NNFELLKNKY NVHIHQKKWE
310 320 330 340 350
KLRVGDFVLL TQDDWVPADL LLLTCDGENS ECFVETMALD GETNLKSKQP
360 370 380 390 400
HPELNKLTKA ASGLANINAQ VTVEDPNIDL YNFEGNLELK NHRNDTIMKY
410 420 430 440 450
PLGPDNVIYR GSILRNTQNV VGMVIFSGEE TKIRMNALKN PRTKAPKLQR
460 470 480 490 500
KINMIIVFMV FVVATISLFS YLGHVLHKKK YIDQNKAWYL FQADAGVAPT
510 520 530 540 550
IMSFIIMYNT VIPLSLYVTM EIIKVVQSKM MEWDIDMYHA ETNTPCESRT
560 570 580 590 600
ATILEELGQV SYIFSDKTGT LTDNKMIFRK FSLCGSSWLH NVDLGNSEDN
610 620 630 640 650
FEDNRDNTNS LRLPPKAHNG SSIDVVSIGD QNVLDRLGFS DAPIEKGHRP
660 670 680 690 700
SLDNFPKSRN SIEYKGNSSA IYTGRPSMRS LFGKDNSHLS KQASVISPSE
710 720 730 740 750
TFSENIKSSF DLIQFIQRYP TALFSQKAKF FFLSLALCHS CLPKKTHNES
760 770 780 790 800
IGEDSIEYQS SSPDELALVT AARDLGYIVL NRNAQILTIK TFPDGFDGEA
810 820 830 840 850
KLENYEILNY IDFNSQRKRM SVLVRMPNQP NQVLLICKGA DNVIMERLHD
860 870 880 890 900
RELAAKKMAD ICTSTKERKD AEAELVLQQR KSLERMVDEE AMARTSLRNS
910 920 930 940 950
LSSVPRASLS LQAVRKSLSM KNSRTRDPEK QIDSIDQFLE TVKKSDQEIG
960 970 980 990 1000
SVVNKSRKSL HKQQIEKYGP RISIDGTHFP NNNVPIDTRK EGLQHDYDTE
1010 1020 1030 1040 1050
ILEHIGSDEL ILNEEYVIER TLQAIDEFST EGLRTLVYAY KWIDIGQYEN
1060 1070 1080 1090 1100
WNKRYHQAKT SLTDRKIKVD EAGAEIEDGL NLLGVTAIED KLQDGVSEAI
1110 1120 1130 1140 1150
EKIRRAGIKM WMLTGDKRET AINIGYSCML IKDYSTVVIL TTTDENIISK
1160 1170 1180 1190 1200
MNAVSQEVDS GNIAHCVVVI DGATMAMFEG NPTYMSVFVE LCTKTDSVIC
1210 1220 1230 1240 1250
CRASPSQKAL MVSNIRNTDP NLVTLAIGDG ANDIAMIQSA DIGVGIAGKE
1260 1270 1280 1290 1300
GLQASRVSDY SIGQFRFLLK LLFVHGRYNY IRTSKFMLCT FYKEITFYFT
1310 1320 1330 1340 1350
QLIYQRYTMF SGSSLYEPWS LSMFNTLFTS LPVLCIGMFE KDLKPMTLLT
1360 1370 1380 1390 1400
VPELYSYGRL SQGFNWLIFM EWVILATTNS LIITFLNVVM WGMSSLSDNT
1410 1420 1430 1440 1450
MYPLGLINFT AIVALINVKS QFVEMHNRNW LAFTSVVLSC GGWLVWCCAL
1460 1470 1480 1490 1500
PILNNTDQIY DVAYGFYNHF GKDITFWCTS LVLALLPITL DIVYKTFKVM
1510 1520 1530 1540 1550
IWPSDSDIFA ELEQKSDIRK KLELGAYSEM RQGWTWDKDP STFTRYTDKV
1560 1570 1580 1590 1600
LSRPRTNSRA SAKTHNSSIY SMSNGNVDHS SKKNFFGNSS KKSSERYEVL
1610 1620 1630 1640 1650
PSGKLIKRPS LKTQSSKDSI GGNITTKLTK KLKLPSRNVE DEDVNQIIQA

RLKDLE
Length:1,656
Mass (Da):188,319
Last modified:November 1, 1997 - v1
Checksum:iA20A823BEB401184
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49705 Genomic DNA. Translation: CAA89798.1.
BK006946 Genomic DNA. Translation: DAA10058.1.
PIRiS54520.
RefSeqiNP_013885.1. NM_001182666.1.

Genome annotation databases

EnsemblFungiiYMR162C; YMR162C; YMR162C.
GeneIDi855197.
KEGGisce:YMR162C.

Similar proteinsi

Entry informationi

Entry nameiATC8_YEAST
AccessioniPrimary (citable) accession number: Q12674
Secondary accession number(s): D6VZY4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 22, 2017
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names