Reviewed, UniProtKB/Swiss-Prot Q12669 (KPYK_ASPNG)

Names and origin

Protein names

Recommended name:
    Pyruvate kinase
      Short name=PK
    EC=2.7.1.40

Gene names

Name:

pkiA

Organism

Aspergillus niger

Taxonomic identifier

5061 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 526	526	Pyruvate kinase		PRO_0000112108
Sites
	Active site	254	1	By similarity
	Metal binding	256	1	Magnesium Potential
	Metal binding	277	1	Magnesium Potential
	Metal binding	278	1	Magnesium Potential
	Binding site	351	1	ADP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q12669-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8B7ED999C46D780C	FASTA	526	58,134
	10 20 30 40 50 60 MAASSSLDHL SNRMKLEWHS KLNTEMVPSK NFRRTSIIGT IGPKTNSVEK INSLRTAGLN 70 80 90 100 110 120 VVRMNFSHGS YQYHQSVIDN AREAAKTQVG RPLAIALDTK GPEIRTGNTP DDKDIPIKQG 130 140 150 160 170 180 HELNITTDEQ YATASDDKNM YLDYKNITKV ISPGKLIYVD DGILSFEVLE VVDDKTIRVR 190 200 210 220 230 240 CLNNGNISSR KGVNLPGTDV DLPALSEKDI ADLKFGVRNK VDMVFASFIR RGSDIRHIRE 250 260 270 280 290 300 VLGEEGKEIQ IIAKIENQQG VNNFDEILEE TDGVMVARGD LGIEIPAPKV FIAQKMMIAK 310 320 330 340 350 360 CNIKGKPVIC ATQMLESMTY NPRPTRAEVS DVANAVLDGA DCVMLSGETA KGNYPNEAVK 370 380 390 400 410 420 MMSETCLLAE VAIPHFNVFD ELRNLAPRPT DTVESIAMAA VSASLELNAG AIVVLTTSGK 430 440 450 460 470 480 TARYLSKYRP VCPIVMVTRN PAASRYSHLY RGVWPFLFPE KKPDFNVKVW QEDVDRRLKW 490 500 510 520 GINHALKLGI INKGDNIVCV QGWRGGMGHT NTVRVVPAEE NLGLAE

References

[1]	"Isolation and characterization of the Aspergillus niger pyruvate kinase gene." de Graaff L., van den Broeck H., Visser J. Curr. Genet. 22:21-27(1992) [PubMed: 1611667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 9089 / N402.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S38698 Genomic DNA. Translation: AAB22392.1.
PIR	S26869.
3D structure databases
HSSP	HSSP built from PDB template 1E0T based on UniProtKB P14178.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.40. 277.
Family and domain databases
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit. G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
ProDom	PD001009. Pyruvate_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_ASPNG

Accession

Primary (citable) accession number: Q12669

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)