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Q12669

- KPYK_ASPNG

UniProt

Q12669 - KPYK_ASPNG

Protein

Pyruvate kinase

Gene

pkiA

Organism
Aspergillus niger
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.By similarity
    Potassium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei63 – 631SubstrateBy similarity
    Metal bindingi65 – 651PotassiumBy similarity
    Metal bindingi67 – 671PotassiumBy similarity
    Metal bindingi98 – 981PotassiumBy similarity
    Metal bindingi99 – 991Potassium; via carbonyl oxygenBy similarity
    Sitei254 – 2541Transition state stabilizerBy similarity
    Metal bindingi256 – 2561MagnesiumSequence Analysis
    Binding sitei279 – 2791Substrate; via amide nitrogenBy similarity
    Metal bindingi280 – 2801MagnesiumBy similarity
    Binding sitei280 – 2801Substrate; via amide nitrogenBy similarity
    Binding sitei312 – 3121SubstrateBy similarity
    Binding sitei351 – 3511ADPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:pkiA
    OrganismiAspergillus niger
    Taxonomic identifieri5061 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 526526Pyruvate kinasePRO_0000112108Add
    BLAST

    Proteomic databases

    PRIDEiQ12669.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ12669.
    SMRiQ12669. Positions 31-515.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12669-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAASSSLDHL SNRMKLEWHS KLNTEMVPSK NFRRTSIIGT IGPKTNSVEK    50
    INSLRTAGLN VVRMNFSHGS YQYHQSVIDN AREAAKTQVG RPLAIALDTK 100
    GPEIRTGNTP DDKDIPIKQG HELNITTDEQ YATASDDKNM YLDYKNITKV 150
    ISPGKLIYVD DGILSFEVLE VVDDKTIRVR CLNNGNISSR KGVNLPGTDV 200
    DLPALSEKDI ADLKFGVRNK VDMVFASFIR RGSDIRHIRE VLGEEGKEIQ 250
    IIAKIENQQG VNNFDEILEE TDGVMVARGD LGIEIPAPKV FIAQKMMIAK 300
    CNIKGKPVIC ATQMLESMTY NPRPTRAEVS DVANAVLDGA DCVMLSGETA 350
    KGNYPNEAVK MMSETCLLAE VAIPHFNVFD ELRNLAPRPT DTVESIAMAA 400
    VSASLELNAG AIVVLTTSGK TARYLSKYRP VCPIVMVTRN PAASRYSHLY 450
    RGVWPFLFPE KKPDFNVKVW QEDVDRRLKW GINHALKLGI INKGDNIVCV 500
    QGWRGGMGHT NTVRVVPAEE NLGLAE 526
    Length:526
    Mass (Da):58,134
    Last modified:November 1, 1996 - v1
    Checksum:i8B7ED999C46D780C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S38698 Genomic DNA. Translation: AAB22392.1.
    PIRiS26869.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S38698 Genomic DNA. Translation: AAB22392.1 .
    PIRi S26869.

    3D structure databases

    ProteinModelPortali Q12669.
    SMRi Q12669. Positions 31-515.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q12669.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG0469.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of the Aspergillus niger pyruvate kinase gene."
      de Graaff L., van den Broeck H., Visser J.
      Curr. Genet. 22:21-27(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 9089 / N402.

    Entry informationi

    Entry nameiKPYK_ASPNG
    AccessioniPrimary (citable) accession number: Q12669
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3