Q12664 (CP51_PENIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Eburicol 14-alpha-demethylase
EC=1.14.13.70

Alternative name(s):
CYPLI
Cytochrome P450 51
Cytochrome P450-14DM
Cytochrome P450-LIA1
Sterol 14-alpha demethylase

Gene names

Name:

CYP51

Organism

Penicillium italicum

Taxonomic identifier

40296 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Penicillium

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Obtusifoliol + 3 O2 + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP+ + 4 H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane Potential. Microsome membrane Potential.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sterol 14-demethylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 515	515	Eburicol 14-alpha-demethylase		PRO_0000052008
Regions
	Transmembrane	11 – 31	21	Helical; Potential
Sites
	Metal binding	459	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12664 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 13679B56F8769255	FASTA	515	58,137
	10 20 30 40 50 60 MDLVPLVTGQ ILGIAYYTTG LFLVSIVLNV IKQLIFYNRK EPPVVFHWIP FIGSTIAYGM 70 80 90 100 110 120 DPYQFFFASR AKYGDIFTFI LLGKKTTVYL GVEGNEFILN GKLKDVNAEE VYGKLTTPVF 130 140 150 160 170 180 GSDVVYDCPN SKLMEQKKFI KYGLSQEALE SYVPLIADET NAYIKSSPNF KGQSGTIDLA 190 200 210 220 230 240 AAMAEITIFT AARTLQGEEV RSKLTSEFAD LFHDLDLGFS PINFMLPWAP LPHNASAIKH 250 260 270 280 290 300 TTYARDLSGN YPSATGSWRR RQRRRQDKSK GTDMISNLMR CVYRDGTPIP DKEIAHMMIT 310 320 330 340 350 360 LLMAGQHSSS AISCWILLRL ASQPEMAEKL HAEQIKNLGA DLPPLQYKDM DKLPLLRNVI 370 380 390 400 410 420 KETLRLHSSI HTLMRKVKNP MPVPGTDFVV PPSHTLLSSP GVTARDERHF RDPLRWDPHR 430 440 450 460 470 480 WESRVEVEDS SDTVDYGYGA VSKGTRSPYL PFGAGRHRCI GEKFAYLNLE VIVATLVREF 490 500 510 RFFNPEGMEG VPDTDYSSLF SRPVQPATVR WEVRS

References

[1]

"Isolation and molecular characterisation of the gene encoding eburicol 14 alpha-demethylase (cYP51) from Penicillium italicum." van Nistelrooy J.G.M., van den Brink J.M., van Kan J.A.L., van Gorcom R.F.M., de Waard M.A. Mol. Gen. Genet. 250:725-733(1996) [PubMed: 8628233] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: W5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49750 Genomic DNA. Translation: CAA89824.1.
PIR	S65578.
3D structure databases
ProteinModelPortal	Q12664.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP51_PENIT

Accession

Primary (citable) accession number: Q12664

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	September 21, 2011
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program