Reviewed, UniProtKB/Swiss-Prot Q12659 (ARO1_PNECA)

Names and origin

Protein names

Recommended name:
    Pentafunctional AROM polypeptide
Including the following 5 domains:
    1- Recommended name:
            3-dehydroquinate synthase
              EC=4.2.3.4
    2- Recommended name:
            3-phosphoshikimate 1-carboxyvinyltransferase
              EC=2.5.1.19
        Alternative name(s):
            5-enolpyruvylshikimate-3-phosphate synthase
              Short name=EPSP synthase
              Short name=EPSPS
    3- Recommended name:
            Shikimate kinase
              EC=2.7.1.71
    4- Recommended name:
            3-dehydroquinate dehydratase
                Short name=3-dehydroquinase
              EC=4.2.1.10
    5- Recommended name:
            Shikimate dehydrogenase
              EC=1.1.1.25

Gene names

Name:

AROM

Organism

Pneumocystis carinii

Taxonomic identifier

4754 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Pneumocystidomycetes › Pneumocystidaceae › Pneumocystis

Protein attributes

Sequence length	1581 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis.
Catalytic activity	3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate = 3-dehydroquinate + phosphate. 3-dehydroquinate = 3-dehydroshikimate + H(2)O. Shikimate + NADP(+) = 3-dehydroshikimate + NADPH. ATP + shikimate = ADP + shikimate 3-phosphate. Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 2/7. Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 3/7. Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 4/7. Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 5/7. Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 6/7.
Subcellular location	Cytoplasm.
Sequence similarities	In the N-terminal section; belongs to the dehydroquinate synthase family. In the 2nd section; belongs to the EPSP synthase family. In the 3rd section; belongs to the shikimate kinase family. In the C-terminal section; belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Kinase Lyase Oxidoreductase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: InterPro 3-dehydroquinate synthase activity Inferred from electronic annotation. Source: InterPro 3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: InterPro shikimate kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1581	1581	Pentafunctional AROM polypeptide		PRO_0000140860
Regions
	Nucleotide binding	875 – 882	8	ATP Potential
	Region	1 – 383	383	3-dehydroquinate synthase
	Region	395 – 840	446	EPSP synthase
	Region	867 – 1042	176	Shikimate kinase
	Region	1043 – 1284	242	3-dehydroquinase
	Region	1297 – 1581	285	Shikimate dehydrogenase
Sites
	Active site	827	1	Potential
	Active site	1189	1	Proton acceptor By similarity
	Active site	1217	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12659-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 417A84356061170F	FASTA	1,581	178,045
	10 20 30 40 50 60 MKEIIKLSIL GKDSIHIGLH LWPHITNELF TCIFSPTYVI ITDSNIETLY IPSFKTYFIS 70 80 90 100 110 120 MAKQRSINSR LLFFTIPPGE KSKSRKTKAL IEDWLLSEKC TRDTVIIAIG GGVIGDLVGY 130 140 150 160 170 180 VSATFMRGVR FIQIPTTLLA MVDSSIGGKN SINTSYGKNA IGTIWQPERI FIDFTFLETL 190 200 210 220 230 240 TEKEFINGIA ELIKTIIIWD ESEFASLENI SEKIAKTVRS MSLTSNKHSK FNEIIKDLKR 250 260 270 280 290 300 YIISSIKIKA HIVSIDEKEK DLRTLLNFGH SIGHAIETVL APYILHGESI SIGMVKEAEL 310 320 330 340 350 360 SRHLGILNPN VVSRLIKCLN TWGLPTSFKD RRFKEVILGK KHLIEDILEI MSIDKKNDSN 370 380 390 400 410 420 NKKIVLLSAI GKTYEKKASS VSDDDIRTIL SQNILLYGIP LNAFQKHTTI TLPGSKSISN 430 440 450 460 470 480 RALILASLSN GICYLKNFLH SDDTYYMLSA LEKLNAAEFK WEQDGDVLVV KGKSGYLENP 490 500 510 520 530 540 QMELYLGNSG TTARFLTSIC TLVQPNSREN HLILTGSNRM KQRPIGPLVD ALKNNGCCIE 550 560 570 580 590 600 YLELENCLPL LIKPKEIGLY GGNINLSATV SSQYVSSILM CSPYAKTQVT LSLIGGKPIS 610 620 630 640 650 660 QPYIDMTISM MSSFGIKVTR SHSKENTYYI PKGCYTCPSE YIIEGDATSA TYPLAIAAIT 670 680 690 700 710 720 GGSCTISNVG SASLQGDSKF SEYILKPMGC EVVQSPTTTY IKGPPKGKLK SLGSINMESM 730 740 750 760 770 780 TDTFLTAAVL ASVAYEESKP YVTKITGISN QRIKECNRIN AMVCELKKFG IEAGELPDGI 790 800 810 820 830 840 YVKALNTSNL PYSVEGINCY NDHRIAMSFS VLACISSKPT TILDKACVNK TWPYWWDILN 850 860 870 880 890 900 STFKVQMKGI EFDLNPTINS SILHHPSECT IFLIGMRGAG KTTLGQLAAN FLGREFIDLD 910 920 930 940 950 960 SIIEEDIKTT ILEFIQKYSW DVFRRKELHF LKTLLTEKKE NYIISCGGGI IETEEARDLL 970 980 990 1000 1010 1020 ISYIEANGIV IHIHRNIQDI IKYLNTDKTR APYQDNIMHV WERRKRWYNL CSSHQFHMTS 1030 1040 1050 1060 1070 1080 TDPTEFKKNI PLNLKSSFNN FLRTITGKNN IFSLILKKKR SYFLSLAFSD LENIFSLLDI 1090 1100 1110 1120 1130 1140 ITAGCDAIEI RIDLFQKPEE IDKYPSLEYI AEKIFLLRQK TSLPLIYTLR TTNHGGSFLS 1150 1160 1170 1180 1190 1200 SEKKLAKEYI LHGAKWGFEF LDIELDIASE LFKTINNSWP YTKIIASYHN IEKPISCDDF 1210 1220 1230 1240 1250 1260 EWIQKYKEAQ HYGHIIKLVG TSSSIEDNFF LEEFKSKFIN KKVPSIIINT GIKGQLSRIM 1270 1280 1290 1300 1310 1320 NTFMTPVTHP SLPSKIAPGQ LSIKEINTAL HIMGLLPEKK YFLFGKPIKH SQSPNIHNLG 1330 1340 1350 1360 1370 1380 FEILGLPYKY QLFETDSISE LKEILHLEEF GGASVTIPLK TNISILLDEI SDHAALIGSV 1390 1400 1410 1420 1430 1440 NTITRTYNNG QYILKGENTD WQGIIKAIKN FNKFEKSFEN FSGFIIGAGG ASRAAIYALL 1450 1460 1470 1480 1490 1500 SLGISPIYLI NRSKDKLNKL YHFFNTNHII PITEYHELNN INFDIRIGIS TIPTDNPIDP 1510 1520 1530 1540 1550 1560 SVLEIAKIFF NLKRKSSEGI FLDMAYGSNT TDLTIIAKAC NWKIIHGLEI LLEQGSEQFL 1570 1580 LWTETYIPYN QVKYAILGPN K

References

[1]	"The cloning and characterization of the arom gene of Pneumocystis carinii." Banerji S., Wakefield A.E., Allen A.G., Maskell D.J., Peters S.E., Hopkin J.M. J. Gen. Microbiol. 139:2901-2914(1993) [PubMed: 8126418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L18918 Unassigned DNA. Translation: AAA17839.1.
PIR	T30832.
3D structure databases
HSSP	HSSP built from PDB template 1NVE based on UniProtKB P07547.
ModBase	Search...
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR002658. DHQ_synth_AroB. IPR016037. DHQ_synth_AroB_sub. IPR001381. DHquinase_I. IPR001986. EPSP_synthase_core. IPR006264. EPSP_synthase_subgroup. IPR008289. Pentafunct_AroM. IPR010110. Shik_DHase_AROM. IPR000623. Shik_kinase. IPR013708. Shikimate_DHase-bd_N. IPR006151. Shikm_DHase/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. G3DSA:3.65.10.10. EPSP_synthase. 1 hit.
PANTHER	PTHR21090:SF1. DHQ_synth_AroB. 1 hit.
Pfam	PF01761. DHQ_synthase. 1 hit. PF01487. DHquinase_I. 1 hit. PF00275. EPSP_synthase. 1 hit. PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. PF01202. SKI. 1 hit. [Graphical view]
PIRSF	PIRSF000514. Pentafunct_AroM. 1 hit.
PRINTS	PR01100. SHIKIMTKNASE.
ProDom	PD005337. DHquinase_I. 1 hit. PD001867. EPSP_synth. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01356. aroA. 1 hit. TIGR01357. aroB. 1 hit. TIGR01093. aroD. 1 hit. TIGR01809. Shik-DH-AROM. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. 1 hit. PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. PS01128. SHIKIMATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARO1_PNECA

Accession

Primary (citable) accession number: Q12659

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 25, 2008
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)