Reviewed, UniProtKB/Swiss-Prot Q12649 (HMDH_PHYBL)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase
      Short name=HMG-CoA reductase
    EC=1.1.1.34

Gene names

Name:

hmgA

Organism

Phycomyces blakesleeanus

Taxonomic identifier

4837 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Fungi incertae sedis › Basal fungal lineages › Mucoromycotina › Mucorales › Mucoraceae › Phycomyces

Protein attributes

Sequence length	1176 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of the sterol biosynthesis.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1176	1176	3-hydroxy-3-methylglutaryl-coenzyme A reductase		PRO_0000114453
Regions
	Transmembrane	35 – 55	21	Potential
	Transmembrane	300 – 320	21	Potential
	Transmembrane	331 – 351	21	Potential
	Transmembrane	623 – 643	21	Potential
	Region	647 – 744	98	Linker By similarity
	Region	745 – 1176	432	Catalytic By similarity
Sites
	Active site	841	1	Charge relay system By similarity
	Active site	972	1	Charge relay system By similarity
	Active site	1048	1	Charge relay system By similarity
	Active site	1146	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	224	1	N-linked (GlcNAc...) Potential
	Glycosylation	238	1	N-linked (GlcNAc...) Potential
	Glycosylation	553	1	N-linked (GlcNAc...) Potential
	Glycosylation	596	1	N-linked (GlcNAc...) Potential
	Glycosylation	670	1	N-linked (GlcNAc...) Potential
	Glycosylation	904	1	N-linked (GlcNAc...) Potential
	Glycosylation	1013	1	N-linked (GlcNAc...) Potential
	Glycosylation	1066	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q12649-1 [UniParc]. Last modified August 29, 2001. Version 2. Checksum: 6BA5C23E828DF35F	FASTA	1,176	127,819
	10 20 30 40 50 60 MSLPNHSGSS AFKSFSYIVG TGIKRAAKLS TRNPIEMIVV VLILSSFSYF YLFNLARTSD 70 80 90 100 110 120 IFSGTVTRLY PTSVYAPTKD HSFSVVDRTA DSTIANNAVK VHLHQIVVSD PKHGVLSRQT 130 140 150 160 170 180 LASVLRFQQM AENEIYVPDS TAVNRFAFNK DLCYKTTLPS SYSSHSSDSN SNSNLNSKTS 190 200 210 220 230 240 PCFAHSPADI WQDEATLLAD KNIRSTIEAN LDTAKNVFGD LQLNATYASS VTLSYAFNTT 250 260 270 280 290 300 GDYREHLADM WKHKVATLPP ADLVSLSNIG QQENVFAWLF IVTRNVIFRV KELIDLADNI 310 320 330 340 350 360 DIIVILVGYI MMIATFISLY VNMRAMGSRY TLATAVVFNG FFSFMLALLT VRALGVDVYP 370 380 390 400 410 420 VVLAEAIPFL AVTIGFERPF KLTKRVFQFS KETPLTKQEI RTTIMRAVDT VALPIARDCF 430 440 450 460 470 480 MEIIVLVLGA KSGISGLEEF CLLSAILLAY DFIIMFTWYT AVLALKLELL RIREINGISA 490 500 510 520 530 540 DDIKKGTKKS TGYIRRTVIK AFSDDHAAGA NTANQKADGP IIGRVKLLMI VGFVVMHIFK 550 560 570 580 590 600 FCSAFQSVGP QVNITEPSIA VVLDQLLEQH KASSQASLPL FVQVFPAMPF HVATVNKSFV 610 620 630 640 650 660 PDAITRPLEA LFDTYAVYIQ HPVISKWLTI ALFVSLFLNT YLFNVAKQPK QIVEQVNQDK 670 680 690 700 710 720 KITNAIESTN NTHIEVTEKQ KPTIQSPGPV VSSAVVMSPN HKRSHNHHHS HSHSHNHHSN 730 740 750 760 770 780 HHQSDIVRPI DECVALVRTP EMLNDEEVIS LVENGKMASY ALEKVLGDLQ RAVGIRRALI 790 800 810 820 830 840 SRASITKTLE ASALPLENYH YDKVMGACCE NVIGYMPIPV GVAGPMNIDG DLIHIPMATT 850 860 870 880 890 900 EGCLVASTAR GCKAINAGGG ASTIVIADGM TRGPCVEFPT ILRAAACKLW IENEGNDIVT 910 920 930 940 950 960 NAFNSTSRFA RLRKLKIALA GKLVFIRFST TTGDAMGMNM ISKGCEKALS IITEHFPDMQ 970 980 990 1000 1010 1020 IISLSGNYCT DKKPAAINWI EGRGKSVVTE AVIPGAIVEK VLKTTVAALV ELNISKNLIG 1030 1040 1050 1060 1070 1080 SAMAGSVGGF NAHAANILTA IYLATGQDPA QNVESSNCIT LMKAVNDTKD LHISCTMPSI 1090 1100 1110 1120 1130 1140 EVGTIGGGTI LPPQQSMLDM LGVRGPHPTE PGKNAQRLAR IICAAVMAGE LSLCAALAAG 1150 1160 1170 HLVKAHMAHN RGTQAPTITS GPAPSTGTEP GTCIKS

References

[1]	"Genes for the metabolism of 3-hydroxy-3-methylglutaryl coenzyme A in the fungus Phycomyces." Ruiz-Albert J., Cerda-Olmedo E., Corrochano L.M. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NRRL 1555.
[2]	"Cloning a segment of the gene encoding 3-hydroxy-3-methyglutaryl coenzyme A reductase in Phycomyces blakesleeanus and Gibberella fujikuroi." Corrochano L.M., Avalos J. Exp. Mycol. 16:167-171(1992) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 836-940. Strain: NRRL 1555.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X58371 Genomic DNA. Translation: CAB97179.1.
PIR	S17345.
3D structure databases
SMR	Q12649. Positions 743-1150.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.34. 278.
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. IPR009023. HMG_CoA_Rdtase_NAD(P)-bd. IPR009029. HMG_CoA_Rdtase_sub-bd. IPR000731. SSD_5TM. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. PS50156. SSD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH_PHYBL

Accession

Primary (citable) accession number: Q12649

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	August 29, 2001
Last modified:	February 9, 2010
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)