Reviewed,
UniProtKB/Swiss-Prot Q12649 (HMDH_PHYBL)
Last modified
November 25, 2008.
Version 57.
History...
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: 3-hydroxy-3-methylglutaryl-coenzyme A reductase Short name=HMG-CoA reductase EC=1.1.1.34 | ||
| Gene names |
| ||
| Organism | Phycomyces blakesleeanus | ||
| Taxonomic identifier | 4837 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Fungi › Fungi incertae sedis › Basal fungal lineages › Mucoromycotina › Mucorales › Mucoraceae › Phycomyces |
Protein attributes
| Sequence length | 1176 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of the sterol biosynthesis. |
| Catalytic activity | (R)-mevalonate + CoA + 2 NADP(+) = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH. |
| Pathway | |
| Subcellular location | Endoplasmic reticulum membrane; Multi-pass membrane protein. |
| Sequence similarities | Belongs to the HMG-CoA reductase family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis |
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Transmembrane |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| PTM | Glycoprotein |
Gene Ontology (GO) | |
| Biological process | cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW coenzyme A metabolic processInferred from electronic annotation. Source: InterPro isoprenoid biosynthetic processInferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: InterPro |
| Molecular function | NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1176 | 1176 | 3-hydroxy-3-methylglutaryl-coenzyme A reductase | PRO_0000114453 | |||||
Regions | |||||||||
| Transmembrane | 35 – 55 | 21 | Potential | ||||||
| Transmembrane | 300 – 320 | 21 | Potential | ||||||
| Transmembrane | 331 – 351 | 21 | Potential | ||||||
| Transmembrane | 623 – 643 | 21 | Potential | ||||||
| Region | 647 – 744 | 98 | Linker By similarity | ||||||
| Region | 745 – 1176 | 432 | Catalytic By similarity | ||||||
Sites | |||||||||
| Active site | 841 | 1 | Charge relay system By similarity | ||||||
| Active site | 972 | 1 | Charge relay system By similarity | ||||||
| Active site | 1048 | 1 | Charge relay system By similarity | ||||||
| Active site | 1146 | 1 | Proton donor By similarity | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 224 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 238 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 553 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 596 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 670 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 904 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 1013 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 1066 | 1 | N-linked (GlcNAc...) Potential | ||||||
Sequences
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References
| [1] | "Genes for the metabolism of 3-hydroxy-3-methylglutaryl coenzyme A in the fungus Phycomyces." Ruiz-Albert J., Cerda-Olmedo E., Corrochano L.M. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NRRL 1555. |
| [2] | "Cloning a segment of the gene encoding 3-hydroxy-3-methyglutaryl coenzyme A reductase in Phycomyces blakesleeanus and Gibberella fujikuroi." Corrochano L.M., Avalos J. Exp. Mycol. 16:167-171(1992) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 836-940. Strain: NRRL 1555. |
Cross-references
Sequence databases | |
|---|---|
| X58371 Genomic DNA. Translation: CAB97179.1. | |
| PIR | S17345. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1HWI based on UniProtKB P04035. |
| ModBase | Search... |
Family and domain databases | |
| InterPro | IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. IPR000731. SSD_5TM. [Graphical view] |
| Gene3D | G3DSA:3.90.770.10. HMG-CoA_red. 1 hit. |
| PANTHER | PTHR10572. HMG-CoA_red. 1 hit. |
| Pfam | PF00368. HMG-CoA_red. 1 hit. [Graphical view] |
| PRINTS | PR00071. HMGCOARDTASE. |
| TIGRFAMs | TIGR00533. HMG_CoA_R_NADP. 1 hit. |
| PROSITE | PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. PS50156. SSD. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | HMDH_PHYBL | ||||||||
| Accession | Primary (citable) accession number: Q12649 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
