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Q12647 (GUNB_NEOPA) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase B

EC=3.2.1.4
Alternative name(s):
Cellulase B
Endo-1,4-beta-glucanase B
Gene names
Name:CELB
OrganismNeocallimastix patriciarum (Rumen fungus)
Taxonomic identifier4758 [NCBI]
Taxonomic lineageEukaryotaFungiNeocallimastigomycotaNeocallimastigomycetesNeocallimastigalesNeocallimastigaceaeNeocallimastix

Protein attributes

Sequence length473 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Rate of hydrolysis of cellulo-oligosaccharides increased with increasing chain length from cellotriose to cellopentaose.

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Contains 2 CBM10 (carbohydrate binding type-10) domains.

Ontologies

Keywords
   DomainRepeat
Signal
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 473456Endoglucanase B
PRO_0000007864

Regions

Domain391 – 42636CBM10 1
Domain437 – 47236CBM10 2
Region18 – 367350Catalytic
Region365 – 38723Linker
Compositional bias376 – 3816Poly-Thr

Sites

Active site1731Proton donor By similarity
Active site2951Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q12647 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: B11D9F171EA33199

FASTA47353,070
        10         20         30         40         50         60 
MKFLNTFSLL SLAIIGSKAM KNISSKELVK DLTIGWSLGN TLDATCFETL DYNKNQIASE 

        70         80         90        100        110        120 
TCWGNVKTTQ ELYYKLSDLG FNTFRIPTTW SGHFGNAPDY KINDQWMKRV HEIVDYAINT 

       130        140        150        160        170        180 
GGYAILNIHH ETWNHAFQKN LESAKKILVA IWKQIAAEFA DYDEHLIFEG MNEPRKVGDP 

       190        200        210        220        230        240 
AEWNGGDYEG WNFVNEMNDL FVKTIRATGG NNALRHLMIP TYAACINDGA INNFKFPSGD 

       250        260        270        280        290        300 
DKVIVSLHSY SPYNFALNNG AGAISNFYDG SEIDWAMNTI NSKFISRGIP VIIGEFGAMN 

       310        320        330        340        350        360 
RNNEDDRERW AEYYIKKATS IGVPCVIWDN GYFEGEGERF GLINRSTLQV VYPKLVNGLI 

       370        380        390        400        410        420 
KGLGNSIKTR TTIRRTTTTT TSQSQPTNND SCFSVNLGYS CCNGCEVEYT DSDGEWGVEN 

       430        440        450        460        470 
GNWCGIKSSC SNTSRICWSE KLGYPCCQNT SSVVYTDNDG KWGVENGNWC GIY 

« Hide

References

[1]"Intronless celB from the anaerobic fungus Neocallimastix patriciarum encodes a modular family A endoglucanase."
Zhou L., Xue G., Orpin C.G., Black G.W., Gilbert H.J., Hazlewood G.P.
Biochem. J. 297:359-364(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z31364 mRNA. Translation: CAA83238.1.
PIRS40507.

3D structure databases

ProteinModelPortalQ12647.
SMRQ12647. Positions 437-472.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH5. Glycoside Hydrolase Family 5.
mycoCLAPEGL5B_NEOPA.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 1 hit.
3.90.1220.10. 2 hits.
InterProIPR002883. CBM10/Dockerin_dom.
IPR009034. Dockerin_dom.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02013. CBM_10. 2 hits.
PF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
SSF64571. SSF64571. 2 hits.
PROSITEPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUNB_NEOPA
AccessionPrimary (citable) accession number: Q12647
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: December 11, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries