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Q12647

- GUNB_NEOPA

UniProt

Q12647 - GUNB_NEOPA

Protein

Endoglucanase B

Gene

CELB

Organism
Neocallimastix patriciarum (Rumen fungus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    • Comment

    Functioni

    Rate of hydrolysis of cellulo-oligosaccharides increased with increasing chain length from cellotriose to cellopentaose.

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei173 – 1731Proton donorBy similarity
    Active sitei295 – 2951NucleophileBy similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH5. Glycoside Hydrolase Family 5.
    mycoCLAPiEGL5B_NEOPA.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase B (EC:3.2.1.4)
    Alternative name(s):
    Cellulase B
    Endo-1,4-beta-glucanase B
    Gene namesi
    Name:CELB
    OrganismiNeocallimastix patriciarum (Rumen fungus)
    Taxonomic identifieri4758 [NCBI]
    Taxonomic lineageiEukaryotaFungiNeocallimastigomycotaNeocallimastigomycetesNeocallimastigalesNeocallimastigaceaeNeocallimastix

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Chaini18 – 473456Endoglucanase BPRO_0000007864Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ12647.
    SMRiQ12647. Positions 437-472.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini391 – 42636CBM10 1Add
    BLAST
    Domaini437 – 47236CBM10 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni18 – 367350CatalyticAdd
    BLAST
    Regioni365 – 38723LinkerAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi376 – 3816Poly-Thr

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.90.1220.10. 2 hits.
    InterProiIPR002883. CBM10/Dockerin_dom.
    IPR009034. Dockerin_dom_fun.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02013. CBM_10. 2 hits.
    PF00150. Cellulase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF64571. SSF64571. 2 hits.
    PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q12647-1 [UniParc]FASTAAdd to Basket

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    MKFLNTFSLL SLAIIGSKAM KNISSKELVK DLTIGWSLGN TLDATCFETL    50
    DYNKNQIASE TCWGNVKTTQ ELYYKLSDLG FNTFRIPTTW SGHFGNAPDY 100
    KINDQWMKRV HEIVDYAINT GGYAILNIHH ETWNHAFQKN LESAKKILVA 150
    IWKQIAAEFA DYDEHLIFEG MNEPRKVGDP AEWNGGDYEG WNFVNEMNDL 200
    FVKTIRATGG NNALRHLMIP TYAACINDGA INNFKFPSGD DKVIVSLHSY 250
    SPYNFALNNG AGAISNFYDG SEIDWAMNTI NSKFISRGIP VIIGEFGAMN 300
    RNNEDDRERW AEYYIKKATS IGVPCVIWDN GYFEGEGERF GLINRSTLQV 350
    VYPKLVNGLI KGLGNSIKTR TTIRRTTTTT TSQSQPTNND SCFSVNLGYS 400
    CCNGCEVEYT DSDGEWGVEN GNWCGIKSSC SNTSRICWSE KLGYPCCQNT 450
    SSVVYTDNDG KWGVENGNWC GIY 473
    Length:473
    Mass (Da):53,070
    Last modified:November 1, 1996 - v1
    Checksum:iB11D9F171EA33199
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z31364 mRNA. Translation: CAA83238.1.
    PIRiS40507.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z31364 mRNA. Translation: CAA83238.1 .
    PIRi S40507.

    3D structure databases

    ProteinModelPortali Q12647.
    SMRi Q12647. Positions 437-472.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH5. Glycoside Hydrolase Family 5.
    mycoCLAPi EGL5B_NEOPA.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    3.90.1220.10. 2 hits.
    InterProi IPR002883. CBM10/Dockerin_dom.
    IPR009034. Dockerin_dom_fun.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02013. CBM_10. 2 hits.
    PF00150. Cellulase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF64571. SSF64571. 2 hits.
    PROSITEi PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Intronless celB from the anaerobic fungus Neocallimastix patriciarum encodes a modular family A endoglucanase."
      Zhou L., Xue G., Orpin C.G., Black G.W., Gilbert H.J., Hazlewood G.P.
      Biochem. J. 297:359-364(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiGUNB_NEOPA
    AccessioniPrimary (citable) accession number: Q12647
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3