SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q12647

- GUNB_NEOPA

UniProt

Q12647 - GUNB_NEOPA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Endoglucanase B
Gene
CELB
Organism
Neocallimastix patriciarum (Rumen fungus)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Rate of hydrolysis of cellulo-oligosaccharides increased with increasing chain length from cellotriose to cellopentaose.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei173 – 1731Proton donor By similarity
Active sitei295 – 2951Nucleophile By similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.
mycoCLAPiEGL5B_NEOPA.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase B (EC:3.2.1.4)
Alternative name(s):
Cellulase B
Endo-1,4-beta-glucanase B
Gene namesi
Name:CELB
OrganismiNeocallimastix patriciarum (Rumen fungus)
Taxonomic identifieri4758 [NCBI]
Taxonomic lineageiEukaryotaFungiNeocallimastigomycotaNeocallimastigomycetesNeocallimastigalesNeocallimastigaceaeNeocallimastix

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717 Reviewed prediction
Add
BLAST
Chaini18 – 473456Endoglucanase B
PRO_0000007864Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ12647.
SMRiQ12647. Positions 437-472.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini391 – 42636CBM10 1
Add
BLAST
Domaini437 – 47236CBM10 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni18 – 367350Catalytic
Add
BLAST
Regioni365 – 38723Linker
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi376 – 3816Poly-Thr

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.90.1220.10. 2 hits.
InterProiIPR002883. CBM10/Dockerin_dom.
IPR009034. Dockerin_dom_fun.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02013. CBM_10. 2 hits.
PF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF64571. SSF64571. 2 hits.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12647-1 [UniParc]FASTAAdd to Basket

« Hide

MKFLNTFSLL SLAIIGSKAM KNISSKELVK DLTIGWSLGN TLDATCFETL    50
DYNKNQIASE TCWGNVKTTQ ELYYKLSDLG FNTFRIPTTW SGHFGNAPDY 100
KINDQWMKRV HEIVDYAINT GGYAILNIHH ETWNHAFQKN LESAKKILVA 150
IWKQIAAEFA DYDEHLIFEG MNEPRKVGDP AEWNGGDYEG WNFVNEMNDL 200
FVKTIRATGG NNALRHLMIP TYAACINDGA INNFKFPSGD DKVIVSLHSY 250
SPYNFALNNG AGAISNFYDG SEIDWAMNTI NSKFISRGIP VIIGEFGAMN 300
RNNEDDRERW AEYYIKKATS IGVPCVIWDN GYFEGEGERF GLINRSTLQV 350
VYPKLVNGLI KGLGNSIKTR TTIRRTTTTT TSQSQPTNND SCFSVNLGYS 400
CCNGCEVEYT DSDGEWGVEN GNWCGIKSSC SNTSRICWSE KLGYPCCQNT 450
SSVVYTDNDG KWGVENGNWC GIY 473
Length:473
Mass (Da):53,070
Last modified:November 1, 1996 - v1
Checksum:iB11D9F171EA33199
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z31364 mRNA. Translation: CAA83238.1.
PIRiS40507.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z31364 mRNA. Translation: CAA83238.1 .
PIRi S40507.

3D structure databases

ProteinModelPortali Q12647.
SMRi Q12647. Positions 437-472.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH5. Glycoside Hydrolase Family 5.
mycoCLAPi EGL5B_NEOPA.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
3.90.1220.10. 2 hits.
InterProi IPR002883. CBM10/Dockerin_dom.
IPR009034. Dockerin_dom_fun.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02013. CBM_10. 2 hits.
PF00150. Cellulase. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF64571. SSF64571. 2 hits.
PROSITEi PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Intronless celB from the anaerobic fungus Neocallimastix patriciarum encodes a modular family A endoglucanase."
    Zhou L., Xue G., Orpin C.G., Black G.W., Gilbert H.J., Hazlewood G.P.
    Biochem. J. 297:359-364(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiGUNB_NEOPA
AccessioniPrimary (citable) accession number: Q12647
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi