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Q12647

- GUNB_NEOPA

UniProt

Q12647 - GUNB_NEOPA

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Protein

Endoglucanase B

Gene

CELB

Organism
Neocallimastix patriciarum (Rumen fungus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Rate of hydrolysis of cellulo-oligosaccharides increased with increasing chain length from cellotriose to cellopentaose.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei173 – 1731Proton donorBy similarity
Active sitei295 – 2951NucleophileBy similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.
mycoCLAPiEGL5B_NEOPA.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase B (EC:3.2.1.4)
Alternative name(s):
Cellulase B
Endo-1,4-beta-glucanase B
Gene namesi
Name:CELB
OrganismiNeocallimastix patriciarum (Rumen fungus)
Taxonomic identifieri4758 [NCBI]
Taxonomic lineageiEukaryotaFungiNeocallimastigomycotaNeocallimastigomycetesNeocallimastigalesNeocallimastigaceaeNeocallimastix

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Chaini18 – 473456Endoglucanase BPRO_0000007864Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ12647.
SMRiQ12647. Positions 437-472.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini391 – 42636CBM10 1Add
BLAST
Domaini437 – 47236CBM10 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni18 – 367350CatalyticAdd
BLAST
Regioni365 – 38723LinkerAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi376 – 3816Poly-Thr

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.90.1220.10. 2 hits.
InterProiIPR002883. CBM10/Dockerin_dom.
IPR009034. Dockerin_dom_fun.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02013. CBM_10. 2 hits.
PF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF64571. SSF64571. 2 hits.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12647-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFLNTFSLL SLAIIGSKAM KNISSKELVK DLTIGWSLGN TLDATCFETL
60 70 80 90 100
DYNKNQIASE TCWGNVKTTQ ELYYKLSDLG FNTFRIPTTW SGHFGNAPDY
110 120 130 140 150
KINDQWMKRV HEIVDYAINT GGYAILNIHH ETWNHAFQKN LESAKKILVA
160 170 180 190 200
IWKQIAAEFA DYDEHLIFEG MNEPRKVGDP AEWNGGDYEG WNFVNEMNDL
210 220 230 240 250
FVKTIRATGG NNALRHLMIP TYAACINDGA INNFKFPSGD DKVIVSLHSY
260 270 280 290 300
SPYNFALNNG AGAISNFYDG SEIDWAMNTI NSKFISRGIP VIIGEFGAMN
310 320 330 340 350
RNNEDDRERW AEYYIKKATS IGVPCVIWDN GYFEGEGERF GLINRSTLQV
360 370 380 390 400
VYPKLVNGLI KGLGNSIKTR TTIRRTTTTT TSQSQPTNND SCFSVNLGYS
410 420 430 440 450
CCNGCEVEYT DSDGEWGVEN GNWCGIKSSC SNTSRICWSE KLGYPCCQNT
460 470
SSVVYTDNDG KWGVENGNWC GIY
Length:473
Mass (Da):53,070
Last modified:November 1, 1996 - v1
Checksum:iB11D9F171EA33199
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z31364 mRNA. Translation: CAA83238.1.
PIRiS40507.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z31364 mRNA. Translation: CAA83238.1 .
PIRi S40507.

3D structure databases

ProteinModelPortali Q12647.
SMRi Q12647. Positions 437-472.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH5. Glycoside Hydrolase Family 5.
mycoCLAPi EGL5B_NEOPA.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
3.90.1220.10. 2 hits.
InterProi IPR002883. CBM10/Dockerin_dom.
IPR009034. Dockerin_dom_fun.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02013. CBM_10. 2 hits.
PF00150. Cellulase. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF64571. SSF64571. 2 hits.
PROSITEi PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Intronless celB from the anaerobic fungus Neocallimastix patriciarum encodes a modular family A endoglucanase."
    Zhou L., Xue G., Orpin C.G., Black G.W., Gilbert H.J., Hazlewood G.P.
    Biochem. J. 297:359-364(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiGUNB_NEOPA
AccessioniPrimary (citable) accession number: Q12647
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 1, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3