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Q12644 (NDUS8_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial

EC=1.6.5.3
EC=1.6.99.3
Alternative name(s):
Complex I-23kD
Short name=CI-23kD
Gene names
Name:nuo21.3c
ORF Names:NCU05009
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora

Protein attributes

Sequence length219 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. May donate electrons to ubiquinone. HAMAP-Rule MF_01351

Catalytic activity

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out). HAMAP-Rule MF_01351

NADH + acceptor = NAD+ + reduced acceptor. HAMAP-Rule MF_01351

Cofactor

Binds 2 4Fe-4S clusters per subunit By similarity. HAMAP-Rule MF_01351

Subunit structure

Complex I is composed of about 40 different subunits.

Subcellular location

Mitochondrion HAMAP-Rule MF_01351.

Sequence similarities

Belongs to the complex I 23 kDa subunit family.

Contains 2 4Fe-4S ferredoxin-type domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion HAMAP-Rule MF_01351
Chain? – 219NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial HAMAP-Rule MF_01351PRO_0000020010

Regions

Domain111 – 140304Fe-4S ferredoxin-type 1
Domain150 – 179304Fe-4S ferredoxin-type 2

Sites

Metal binding1201Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1231Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1261Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1301Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1591Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1621Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1651Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1691Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q12644 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 650E1CF3A7E61930

FASTA21924,902
        10         20         30         40         50         60 
MLTTTASSAA AVARQLTTRR VIAPSFVSQA IRTYATPAGP PPKGFRIPTP KTWDQEEEHV 

        70         80         90        100        110        120 
LDKNGRYFLL TEMFRGMYVA MEQFFRPPYT IYYPFEKGPI SPRFRGEHAL RRYPSGEERC 

       130        140        150        160        170        180 
IACKLCEAVC PAQAITIEAE ERADGSRRTT RYDIDMTKCI YCGFCQESCP VDAIVESPNA 

       190        200        210 
EYATETREEL LYNKEKLLSN GDKWEPELAA AIRADSPYR 

« Hide

References

« Hide 'large scale' references
[1]"Primary structure of a ferredoxin-like iron-sulfur subunit of complex I from Neurospora crassa."
Duarte M., Finel M., Videira A.
Biochim. Biophys. Acta 1275:151-153(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X95547 mRNA. Translation: CAA64794.1.
CM002241 Genomic DNA. Translation: ESA42320.1.
CM002241 Genomic DNA. Translation: ESA42321.1.
RefSeqXP_955936.1. XM_950843.2.
UniGeneNcr.9031.

3D structure databases

ProteinModelPortalQ12644.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5141.NCU05009.1.

Protein family/group databases

TCDB3.D.1.6.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000001567; EFNCRP00000001567; EFNCRG00000001565.
GeneID3872083.
KEGGncr:NCU05009.

Phylogenomic databases

eggNOGCOG1143.
HOGENOMHOG000228289.
KOK03941.
OrthoDBEOG7P5TB6.

Family and domain databases

HAMAPMF_01351. NDH1_NuoI.
InterProIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsTIGR01971. NuoI. 1 hit.
PROSITEPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNDUS8_NEUCR
AccessionPrimary (citable) accession number: Q12644
Secondary accession number(s): Q7RV45, V5INI7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families