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Q12644

- NDUS8_NEUCR

UniProt

Q12644 - NDUS8_NEUCR

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Protein
NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial
Gene
nuo21.3c, NCU05009
Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. May donate electrons to ubiquinone.UniRule annotation

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).UniRule annotation
NADH + acceptor = NAD+ + reduced acceptor.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi120 – 1201Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi123 – 1231Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi126 – 1261Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi130 – 1301Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi159 – 1591Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi162 – 1621Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi165 – 1651Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi169 – 1691Iron-sulfur 1 (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Protein family/group databases

TCDBi3.D.1.6.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-23kD
Short name:
CI-23kD
Gene namesi
Name:nuo21.3c
ORF Names:NCU05009
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 5, Linkage Group VI

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
  2. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 219NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrialUniRule annotationPRO_0000020010
Transit peptidei1 – ?MitochondrionUniRule annotation

Interactioni

Subunit structurei

Complex I is composed of about 40 different subunits.

Protein-protein interaction databases

STRINGi5141.NCU05009.1.

Structurei

3D structure databases

ProteinModelPortaliQ12644.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini111 – 140304Fe-4S ferredoxin-type 1
Add
BLAST
Domaini150 – 179304Fe-4S ferredoxin-type 2
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiCOG1143.
HOGENOMiHOG000228289.
KOiK03941.
OrthoDBiEOG7P5TB6.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12644-1 [UniParc]FASTAAdd to Basket

« Hide

MLTTTASSAA AVARQLTTRR VIAPSFVSQA IRTYATPAGP PPKGFRIPTP    50
KTWDQEEEHV LDKNGRYFLL TEMFRGMYVA MEQFFRPPYT IYYPFEKGPI 100
SPRFRGEHAL RRYPSGEERC IACKLCEAVC PAQAITIEAE ERADGSRRTT 150
RYDIDMTKCI YCGFCQESCP VDAIVESPNA EYATETREEL LYNKEKLLSN 200
GDKWEPELAA AIRADSPYR 219
Length:219
Mass (Da):24,902
Last modified:November 1, 1996 - v1
Checksum:i650E1CF3A7E61930
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X95547 mRNA. Translation: CAA64794.1.
CM002241 Genomic DNA. Translation: ESA42320.1.
CM002241 Genomic DNA. Translation: ESA42321.1.
RefSeqiXP_955936.1. XM_950843.2.
UniGeneiNcr.9031.

Genome annotation databases

EnsemblFungiiEFNCRT00000001567; EFNCRP00000001567; EFNCRG00000001565.
GeneIDi3872083.
KEGGincr:NCU05009.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X95547 mRNA. Translation: CAA64794.1 .
CM002241 Genomic DNA. Translation: ESA42320.1 .
CM002241 Genomic DNA. Translation: ESA42321.1 .
RefSeqi XP_955936.1. XM_950843.2.
UniGenei Ncr.9031.

3D structure databases

ProteinModelPortali Q12644.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5141.NCU05009.1.

Protein family/group databases

TCDBi 3.D.1.6.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFNCRT00000001567 ; EFNCRP00000001567 ; EFNCRG00000001565 .
GeneIDi 3872083.
KEGGi ncr:NCU05009.

Phylogenomic databases

eggNOGi COG1143.
HOGENOMi HOG000228289.
KOi K03941.
OrthoDBi EOG7P5TB6.

Family and domain databases

HAMAPi MF_01351. NDH1_NuoI.
InterProi IPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view ]
Pfami PF12838. Fer4_7. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01971. NuoI. 1 hit.
PROSITEi PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of a ferredoxin-like iron-sulfur subunit of complex I from Neurospora crassa."
    Duarte M., Finel M., Videira A.
    Biochim. Biophys. Acta 1275:151-153(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiNDUS8_NEUCR
AccessioniPrimary (citable) accession number: Q12644
Secondary accession number(s): Q7RV45, V5INI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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