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Q12644

- NDUS8_NEUCR

UniProt

Q12644 - NDUS8_NEUCR

Protein

NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial

Gene

nuo21.3c

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. May donate electrons to ubiquinone.

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi120 – 1201Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi123 – 1231Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi126 – 1261Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi130 – 1301Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi159 – 1591Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi162 – 1621Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi165 – 1651Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi169 – 1691Iron-sulfur 1 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Protein family/group databases

    TCDBi3.D.1.6.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    Complex I-23kD
    Short name:
    CI-23kD
    Gene namesi
    Name:nuo21.3c
    ORF Names:NCU05009
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 5, Linkage Group VI

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell
    2. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 219NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrialPRO_0000020010
    Transit peptidei1 – ?Mitochondrion

    Interactioni

    Subunit structurei

    Complex I is composed of about 40 different subunits.

    Protein-protein interaction databases

    STRINGi5141.NCU05009.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12644.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini111 – 140304Fe-4S ferredoxin-type 1Add
    BLAST
    Domaini150 – 179304Fe-4S ferredoxin-type 2Add
    BLAST

    Sequence similaritiesi

    Belongs to the complex I 23 kDa subunit family.Curated
    Contains 2 4Fe-4S ferredoxin-type domains.Curated

    Keywords - Domaini

    Repeat, Transit peptide

    Phylogenomic databases

    eggNOGiCOG1143.
    HOGENOMiHOG000228289.
    KOiK03941.
    OrthoDBiEOG7P5TB6.

    Family and domain databases

    HAMAPiMF_01351. NDH1_NuoI.
    InterProiIPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR010226. NADH_quinone_OxRdtase_chainI.
    [Graphical view]
    PfamiPF12838. Fer4_7. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01971. NuoI. 1 hit.
    PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q12644-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLTTTASSAA AVARQLTTRR VIAPSFVSQA IRTYATPAGP PPKGFRIPTP    50
    KTWDQEEEHV LDKNGRYFLL TEMFRGMYVA MEQFFRPPYT IYYPFEKGPI 100
    SPRFRGEHAL RRYPSGEERC IACKLCEAVC PAQAITIEAE ERADGSRRTT 150
    RYDIDMTKCI YCGFCQESCP VDAIVESPNA EYATETREEL LYNKEKLLSN 200
    GDKWEPELAA AIRADSPYR 219
    Length:219
    Mass (Da):24,902
    Last modified:November 1, 1996 - v1
    Checksum:i650E1CF3A7E61930
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X95547 mRNA. Translation: CAA64794.1.
    CM002241 Genomic DNA. Translation: ESA42320.1.
    CM002241 Genomic DNA. Translation: ESA42321.1.
    RefSeqiXP_955936.1. XM_950843.2.
    UniGeneiNcr.9031.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000001567; EFNCRP00000001567; EFNCRG00000001565.
    GeneIDi3872083.
    KEGGincr:NCU05009.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X95547 mRNA. Translation: CAA64794.1 .
    CM002241 Genomic DNA. Translation: ESA42320.1 .
    CM002241 Genomic DNA. Translation: ESA42321.1 .
    RefSeqi XP_955936.1. XM_950843.2.
    UniGenei Ncr.9031.

    3D structure databases

    ProteinModelPortali Q12644.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU05009.1.

    Protein family/group databases

    TCDBi 3.D.1.6.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000001567 ; EFNCRP00000001567 ; EFNCRG00000001565 .
    GeneIDi 3872083.
    KEGGi ncr:NCU05009.

    Phylogenomic databases

    eggNOGi COG1143.
    HOGENOMi HOG000228289.
    KOi K03941.
    OrthoDBi EOG7P5TB6.

    Family and domain databases

    HAMAPi MF_01351. NDH1_NuoI.
    InterProi IPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR010226. NADH_quinone_OxRdtase_chainI.
    [Graphical view ]
    Pfami PF12838. Fer4_7. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01971. NuoI. 1 hit.
    PROSITEi PS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of a ferredoxin-like iron-sulfur subunit of complex I from Neurospora crassa."
      Duarte M., Finel M., Videira A.
      Biochim. Biophys. Acta 1275:151-153(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

    Entry informationi

    Entry nameiNDUS8_NEUCR
    AccessioniPrimary (citable) accession number: Q12644
    Secondary accession number(s): Q7RV45, V5INI7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3