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Protein

NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial

Gene

nuo21.3c

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. May donate electrons to ubiquinone.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi120Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi123Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi126Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi130Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi159Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi162Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi165Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi169Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Protein family/group databases

TCDBi3.D.1.6.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-23kD
Short name:
CI-23kD
Gene namesi
Name:nuo21.3c
ORF Names:NCU05009
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 5, Linkage Group VI

Organism-specific databases

EuPathDBiFungiDB:NCU05009.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000020010? – 219NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial
Transit peptidei1 – ?Mitochondrion

Interactioni

Subunit structurei

Complex I is composed of about 40 different subunits.

Structurei

3D structure databases

ProteinModelPortaliQ12644.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini111 – 1404Fe-4S ferredoxin-type 1Add BLAST30
Domaini150 – 1794Fe-4S ferredoxin-type 2Add BLAST30

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated
Contains 2 4Fe-4S ferredoxin-type domains.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

HOGENOMiHOG000228289.
InParanoidiQ12644.
KOiK03941.
OrthoDBiEOG092C45DR.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12644-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTTTASSAA AVARQLTTRR VIAPSFVSQA IRTYATPAGP PPKGFRIPTP
60 70 80 90 100
KTWDQEEEHV LDKNGRYFLL TEMFRGMYVA MEQFFRPPYT IYYPFEKGPI
110 120 130 140 150
SPRFRGEHAL RRYPSGEERC IACKLCEAVC PAQAITIEAE ERADGSRRTT
160 170 180 190 200
RYDIDMTKCI YCGFCQESCP VDAIVESPNA EYATETREEL LYNKEKLLSN
210
GDKWEPELAA AIRADSPYR
Length:219
Mass (Da):24,902
Last modified:November 1, 1996 - v1
Checksum:i650E1CF3A7E61930
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95547 mRNA. Translation: CAA64794.1.
CM002241 Genomic DNA. Translation: ESA42320.1.
CM002241 Genomic DNA. Translation: ESA42321.1.
RefSeqiXP_011394933.1. XM_011396631.1.
XP_011394934.1. XM_011396632.1.

Genome annotation databases

EnsemblFungiiESA42320; ESA42320; NCU05009.
ESA42321; ESA42321; NCU05009.
GeneIDi3872083.
KEGGincr:NCU05009.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95547 mRNA. Translation: CAA64794.1.
CM002241 Genomic DNA. Translation: ESA42320.1.
CM002241 Genomic DNA. Translation: ESA42321.1.
RefSeqiXP_011394933.1. XM_011396631.1.
XP_011394934.1. XM_011396632.1.

3D structure databases

ProteinModelPortaliQ12644.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi3.D.1.6.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiESA42320; ESA42320; NCU05009.
ESA42321; ESA42321; NCU05009.
GeneIDi3872083.
KEGGincr:NCU05009.

Organism-specific databases

EuPathDBiFungiDB:NCU05009.

Phylogenomic databases

HOGENOMiHOG000228289.
InParanoidiQ12644.
KOiK03941.
OrthoDBiEOG092C45DR.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDUS8_NEUCR
AccessioniPrimary (citable) accession number: Q12644
Secondary accession number(s): Q7RV45, V5INI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.